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Citations for GE Life Sciences :
451 - 500 of 731 citations for L Aspartic Acid N T Boc B Bz Ester 13C4 97 99%; 15N 97 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Membranes were blocked in non-fat 4% milk in TBS/T and incubated with primary and HRP-conjugated secondary (GE Healthcare) antibodies ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The membranes were washed three times with TBS-T and protein bands were visualized using the CDP-star detection reagent (GE Healthcare). Intensity was detected and quantitatively analyzed by the Fluor-S MAX multi-imager system (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... All blots were washed 4X with PBS-T and then developed with a Amersham ECL Western Blotting Detection Kit (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... the wells were washed with PBS containing 0.05 % Tween®20 (PBS-T) and incubated with HRP-conjugated antibodies (GE healthcare) for 3 hours at RT ...
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bioRxiv - Biochemistry 2022Quote: ... The membrane was washed twice in PBS-T and three times in PBS and readout was enabled using the ECL Select Western Blotting Detection Reagent (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... The membrane was washed twice in PBS-T before being visualized on a Typhoon Trio Scanner (GE Healthcare, Piscataway, NJ, USA) and quantified with ImageJ (Abramoff et al. ...
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bioRxiv - Immunology 2022Quote: ... RPAPGST(GalNAc)APPAHG and RPAPGS(GalNAc)T(GalNAc)APPAHG with immobilized antibody 14A and 16A were determined by surface plasmon resonance on a Biacore T200 (GE Healthcare) instrument ...
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bioRxiv - Immunology 2022Quote: ... Membranes were then incubated with the corresponding HRP-tagged secondary antibodies in BSA (5% w/v) PBS-T for 1 hour before visualisation using Amersham ECL prime detection reagent (GE healthcare) and a G:Box Chemi XX6 system (Syngene) ...
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bioRxiv - Molecular Biology 2022Quote: ... diluted 1:10,000 in TBS-T with 5% powdered milk and finally washed 3x in TBS-T for 10 min before adding ECL substrate (Amersham) for detection of HRP activity using a CCD camera (ImageQuant, GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... and revealed manually on X-Ray films (Fuji; FOMA LP-T and FOMAFIX) or using a digital Amersham Imager 680 (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated for 1 h at room temperature with secondary antibody (anti-mouse IgG, 1:10,000 in 3% BSA/TBS-T; GE Healthcare, #RPN4201) linked to horseradish peroxidase ...
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bioRxiv - Immunology 2023Quote: ... The membrane was then washed several times in 0.05% TBS-T and proteins were detected using enhanced chemiluminescence (ECL) reagent (GE Healthcare; RPN2106). Antibodies used include ICAM-1 (Cell Signaling Technologies ...
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bioRxiv - Immunology 2023Quote: ... the blot was washed three times every 5 min using 1X TBS-T before incubation with HRP-conjugated goat-anti-rabbit (GE Healthcare) or goat-anti-mouse (GE health care ...
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bioRxiv - Systems Biology 2023Quote: ... After two additional washing steps with TBS-T and one with TBS the blots were developed by incubation with Amersham ECL Western Blotting Detection Reagents (GE Healthcare) for 2 min and detected on an ImageQuant (GE Healthcare) ...
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bioRxiv - Neuroscience 2023Quote: ... the rabbits were anesthetized via 2% isoflurane inhalation and imaged consecutively with clinical SPECT/CT scanner (Siemens symbia T16 SPECT/CT) and a 3.0 T clinical MRI scanner (Signa HDxt, GE Healthcare, USA) at 6 and 36 h post-injection ...
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bioRxiv - Neuroscience 2023Quote: ... the thoracoabdominal aorta of the rabbits was examined by MRI fast spin-echo (FSE) T2W imaging using a 3.0 T MR scanner (Signa HDxt, GE Healthcare, USA) and an 8-channel phased abdominal coil ...
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bioRxiv - Immunology 2020Quote: ... flow channels were activated for 7 minutes with a 1-to-1 mixture of 0.1 M N-hydroxysuccinimide (GE healthcare) and 0.1 M 3-(N,N-dimethylamino ...
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bioRxiv - Biochemistry 2020Quote: ... 100 pmoles of DNA samples were blotted on a positively charged nylon membrane (Amersham Hybond-N+, GE Healthcare Life Science). The DNA was then UV crosslinked to the membrane (254 nm ...
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bioRxiv - Microbiology 2020Quote: ... The VSGsur N-terminal domain was purified by gel filtration on a HiLoad 16/600 Superdex 200pg column (GE Healthcare) in 10 mM Tris.Cl ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 μg of total RNA from each sample was analysed through 1.2% agarose-formaldehyde gels and transferred to HybondTM-N+ membrane (GE Healthcare) by neutral transfer ...
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bioRxiv - Immunology 2022Quote: ... pooled plasma (n=14-15 per pool) was injected onto a Superose 6 HR 10/300 GL column (GE Healthcare) and eluted at a constant flow rate of 500 μL/min ...
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bioRxiv - Microbiology 2020Quote: ... The GST tag of N-terminal GST-6xHis-RhoGAP domain was removed by addition of PreScission protease (GE healthcare Lifesciences) directly to the glutathione beads followed by incubation overnight at 4°C to obtain His-tagged RhoGAP ...
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bioRxiv - Biochemistry 2022Quote: ... Small RNAs were separated on a 15% sequencing gel and transferred to Amersham Hybond-N+ membranes (GE Healthcare, cat# RPN303B) using a Trans-blot SD semi-dry electrophoretic transfer cell (Bio-Rad ...
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bioRxiv - Genetics 2022Quote: ... Gels were washed for 15 min in 0.25 N HCl before an overnight transfer to a charged nylon membrane (Hybond-XL, GE Healthcare) in 0.4 N NaOH ...
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bioRxiv - Plant Biology 2022Quote: ... DNA-protein complexes were resolved in a 6% polyacrylamide gel and transferred to a charged Hybond-N+ membrane (GE Healthcare). The membrane was cross-linked by UV (1200 mJ) ...
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bioRxiv - Pathology 2022Quote: ... High-molecular weight RNAs were separated in 1 % formaldehyde agarose gels and transferred to Hybond-N+ membranes (GE Healthcare, UK) for RNA hybridization in membranes ...
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bioRxiv - Plant Biology 2022Quote: ... HinfI-restricted genomic DNA was separated on a 1.2 % agarose gel and transferred onto Hybond-N+ nylon membranes (GE Healthcare) by alkaline transfer ...
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bioRxiv - Genomics 2023Quote: ... 2 µL of gDNA (containing indicated amounts of DNA) was denatured with 0.5 N NaOH for 15 minutes then spotted on a nitrocellulose blotting membrane (GE Healthcare) and cross-linked at 120,000 microjoules for 20 min using a Stratalinker® UV Crosslinker 1800 (Stratagene) ...
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bioRxiv - Microbiology 2023Quote: ... The transfer of the RNA from the agarose gel to the nylon membrane (Amersham Hyband™-N+, GE Healthcare, #RPN303B) was done as via capillary transfer of RNA from the denaturing agarose gel to the nylon membrane (Sambrook ...
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bioRxiv - Cell Biology 2023Quote: ... Antibodies were affinity purified against DLI-1(1-368) coupled to a 1-mL HiTrap N-hydroxysuccinimide column (GE Healthcare) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... The fragments were separated on 0.8% agarose gel and transferred by capillarity to the Amersham Hybond N+ membrane (GE Healthcare). DNA hybridization was performed with the Amersham AlkPhos Direct Hybridization Buffer ...
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bioRxiv - Genomics 2023Quote: ... 50 ml of sea water was filtered through a 1.2 μm pore-size acrodisc (FP 30/1.2 CA-S cat N° 10462260 Whatman GE Healthcare Sciences) and used to inoculate culture flasks with 10 ml of filtrate each ...
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bioRxiv - Cell Biology 2021Quote: ... Knockdowns of CDK1 was performed by using SMARTpool reagents (L-003224-00-0005; GE Healthcare) and ON-TARGETplus nontargeting siRNA (GE Healthcare ...
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bioRxiv - Bioengineering 2020Quote: ... CHO supernatants were flowed through columns packed with Protein L or Protein A (GE Healthcare) in 50mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... coli M55244 cells (ATCC) and purified using Protein L resin (GE Healthcare Cat no. 17547802) with gravity flow affinity chromatography ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was loaded on a His-Trap FF column (GE Healthcare, Chicago, I L) equilibrated with lysis buffer and washed with 3 CV lysis buffer containing 20 mM imidazole and washed again with 3 CV lysis buffer containing 50 mM imidazole ...
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bioRxiv - Cancer Biology 2019Quote: ... The fluorophore conjugated CMP-sialic acids were then purified on a HiTrap® Q HP (GE Healthcare) column and eluted with a 0-100% gradient of NaCl elution buffer (150 NaCl ...
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bioRxiv - Microbiology 2021Quote: ... Nucleic acid contaminants were removed by directly binding CPSF4-YTH onto a 5 ml heparin (GE healthcare) and eluting by a 40 ml step NaCl gradient (150 mM to 2M ...
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bioRxiv - Cell Biology 2022Quote: ... URA3: CaUra3pXhoI and CaURA3m81) and Amersham ECL Direct Nucleic Acid Labelling and Detection System kit (GE Healthcare UK Limited ...
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bioRxiv - Biophysics 2022Quote: ... The filtered supernatant included NMnPP protein was applied to a nickel-nitrilotriacetic acid (Ni-NTA, GE Healthcare) affinity column pre-equilibrated with buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... diluted two-fold against fresh buffer B and then manually applied at room temperature to a 5 mL HiTrap Heparin cation exchange column (GE Healthcare # 17040701) that had been pre-equilibrated in buffer B ...
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bioRxiv - Microbiology 2020Quote: ... 300 ml NaCl and eluted via a gradient of increasing imidazole (0-500 mM, Buffer B) using a ÄKTAPurifier™ System (GE Healthcare). After this first purification ...
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bioRxiv - Plant Biology 2022Quote: ... induced for 3 h induction by IPTG (isopropyl b-D-thiogalactopyranoside) and soluble extracts were purified using GST-sepharose 4B (GE Healthcare, Sweden) as described elsewhere (Geisler et al. ...
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bioRxiv - Immunology 2021Quote: ... iPSC-moDC and MDM were washed with T cell expansion medium (RPMI supplemented with 10% HyClone FBS (GE Healthcare, Chicago, IL, USA), 1000 U/mL Penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were further washed three times for five minutes in PBS-T before being visualised using ECL kit (GE Healthcare Cat. #RPN2232) and ChemiDoc Imaging System (BioRad) ...
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bioRxiv - Microbiology 2020Quote: 3D structured illumination microscopy of fixed T cells cells was performed with a commercial Deltavision OMXv4.0 BLAZE microscope (GE Healthcare, Amersham, UK) using a 60x ...
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bioRxiv - Cell Biology 2019Quote: ... Membranes were then washed again for 3 × 15 min in PBS-T and signals were detected with ECL Western Blotting Detection Reagent (GE Healthcare, #RPN2106) and acquired with Chemidoc MP Imaging System (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Before revelation membranes were washed again 3 times in TBS-T for 5min and then incubated in ECL™ Western Blotting Detection Reagent (RPN2209, GE Healthcare) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... they were soaked in TBS-t for 30 min at room temperature with HRP-conjugated anti-mouse (GE Healthcare, Chicago, IL, USA) or anti-rabbit antibody (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... the following HRP conjugated secondary antibodies were used: for Cas9 - anti-mouse (1:10,000 in 1% milk PBS-T, GE Healthcare, code NA931V); for γ-H2A - anti-rabbit (1:2000 in 1% milk/PBS-T ...