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Citations for GE Life Sciences :
451 - 500 of 5990 citations for Elongator complex protein 1 ELP1 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The proteins were further incubated with protein A/G-sepharose beads (GE Healthcare) for 2 hours ...
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bioRxiv - Biochemistry 2022Quote: ... Fc-fused ACE2 proteins were purified on HiTrap Protein A column (GE Healthcare) and eluted with 50 mM Tris pH 7.2 ...
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bioRxiv - Immunology 2022Quote: ... fusion protein was purified from cell supernatant using protein A column (GE healthcare). After digestion with TEV protease ...
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bioRxiv - Neuroscience 2021Quote: ... placed in 5% milk PBST plus 1:2500 mouse specific HRP antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Neuroscience 2021Quote: ... anti-mouse or anti-rat secondary antibodies (1:4000 in blocking buffer, GE Healthcare) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... and IgG anti-rabbit horseradish peroxidase–conjugated secondary antibody (1:10,000; GE Healthcare UK). For whole cerebellum and cerebellar slices ...
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bioRxiv - Cancer Biology 2020Quote: ... horseradish peroxidase (HRP)-conjugated anti-mouse antibody (NA931, GE Healthcare Life Sciences, 1:10,000) or HRP-conjugated anti-rabbit antibody (NA934V ...
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bioRxiv - Plant Biology 2020Quote: ... we used a peroxidase-labeled anti-rabbit antibody (NIF824, GE Healthcare; 1:5000 dilution). Immunodetection was performed by incubating the membranes in the Western BLoT Quant HRP Substrate (Takara ...
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bioRxiv - Microbiology 2020Quote: ... a 1:10000 dilution of HRP-conjugated donkey anti-rabbit antibody (GE Healthcare, NA934), and ECL detection reagent ...
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bioRxiv - Biophysics 2020Quote: ... and a secondary anti-mouse IgG HRP-conjugated antibody (1:1000, #NA931, GE Healthcare). The soluble fraction was found to contain more AβM(E22G)-mCherry than the insoluble fraction (Supplementary Figure 5. ...
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bioRxiv - Cell Biology 2021Quote: ... followed by the corresponding Horseradish Peroxidase (HRP) conjugated secondary antibodies (1:5000, GE Healthcare) and visualized using the ECL Plus Western Blotting detection system (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... followed by anti-rabbit and mouse antibodies coupled to HRP (1:2000, GE Healthcare).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 50 μl of a 1:1000 dilution of anti-rabbit HRP antibody (GE Healthcare) was added per well ...
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bioRxiv - Biophysics 2020Quote: ... and a secondary anti-mouse IgG HRP-conjugated antibody (1:1000, #NA931, GE Healthcare). The membrane was dried and reprobed with an anti-mCherry antibody (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... Primary antibody labelling was done for 1 hour using mouse anti-BrdU (GE Healthcare) and rat anti-HA (Roche ...
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Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneuronsbioRxiv - Neuroscience 2022Quote: ... Secondary antibodies used: anti-mouse-IgG-Horseradish Peroxidase (GE Healthcare, Buckinghamshire, UK, 1:4000).
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bioRxiv - Neuroscience 2024Quote: ... The secondary antibody was goat anti-rabbit IgG at 1:5000 dilution (GE Healthcare). Biotinylated proteins were blotted using HRP- conjugated Streptavidin at 1:1000 dilution (Thermo ...
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bioRxiv - Molecular Biology 2024Quote: ... Anti-GST primary antibody was used at 1:1,000 (GE Healthcare 27-4577-01) and secondary antibody was conjugate to Alexa 680 nm dye at 1:10,000 ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies used were HRP-linked donkey anti-mouse IgG (1:5000, GE Healthcare) and HRP-linked donkey anti-rabbit (1:5000 ...
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bioRxiv - Plant Biology 2024Quote: ... and ECL anti-mouse IgG horseradish peroxidase-linked whole antibody (1:1,000; GE Healthcare).
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bioRxiv - Biophysics 2020Quote: ... Proteins were purified using an AKTA FPLC with 1 mL nickel-charged HisTrap columns (GE Healthcare Life Sciences) for affinity chromatography of the His-tagged proteins ...
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bioRxiv - Immunology 2021Quote: ... Fab and partially cleaved IgG were applied on 1-mL HiTrap Protein L column (GE Healthcare Life Science). After loading ...
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bioRxiv - Bioengineering 2020Quote: ... Supernatants were collected by centrifugation and purified using a 1 ml HiTrap Protein A HP column (GE Healthcare) for IgG expression and 5 ml kappa-select column (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM DTT with USP7 proteins purified as described previously 62 using Glutathione Sepharose 4B beads (GE Healthcare). After elution the GST tag was removed under dialysis using 3C protease and the sample fractionated by PorosXQ anion exchange (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... and the soluble MED25 ACID protein was purified on 1 ml beads loaded with Ni-NTA (GE Healthcare). The bound protein was washed extensively with loading buffer containing 25 mM imidazole and eluted with a 250 mM imidazole pH 8.
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bioRxiv - Cell Biology 2021Quote: ... DNA fragments encoding APC proteins were inserted into pGEX-5X-1 (GE Healthcare Life Sciences, Pittsburgh, PA, USA). For in vitro transcription and translation reaction ...
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bioRxiv - Immunology 2020Quote: ... run at a flow rate of 0.5-1 mL/min on Äkta Start protein purification system (GE Healthcare). Antibodies were eluted using 0.1M glycine pH 2 ...
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bioRxiv - Plant Biology 2020Quote: ... Desalting columns (NAP-5 and PD-10) and N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)proprionamide (HPDP-biotin) were purchased from GE Healthcare and Pierce ...
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bioRxiv - Biophysics 2020Quote: ... 3’ biotinylated RNA constructs (linked via an extended TEG spacer arm) were purchased from Integrated DNA technologies (Baulkham Hills, NSW) and immobilised on a Biotin CAPture chip (GE Healthcare) in 10 mM sodium acetate pH 4.8 ...
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bioRxiv - Biochemistry 2021Quote: ... The click-reaction was performed by incubation for 30 min at 20 °C in the dark with vigorous intermittent shaking and the free biotin was removed from the clicked extracts using PD MiniTrap G-25 column (GE Healthcare) equilibrated with IPD buffer ...
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bioRxiv - Immunology 2020Quote: ... The antibodies were biotinylated using EZ-Link NHS-LC-LC-biotin (Pierce) and conjugated to one of several fluorophores - Cy5 Monoreactive dye (GE Healthcare), AlexaFluor 405 or AlexaFluor 647 NHS esters (ThermoFisher ...
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bioRxiv - Biophysics 2024Quote: ... NCX1 was eluted in buffer A supplemented with 50 mM biotin and further purified by size-exclusion chromatography on a Superdex200 10/300 GL column (GE Healthcare). For the generation of NCX1-Fab 2E4 complex ...
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bioRxiv - Molecular Biology 2021Quote: ... 300 µg of the prepared lysate was incubated with the appropriate antibody (3 µg) and pulled down using protein A Sepharose 6MB (GE healthcare # 17-0469-01). All primary antibodies used in this study are listed in table T3 ...
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bioRxiv - Biophysics 2021Quote: ... Amplified phage particles at 10-fold dilution were assayed against 20 nM biotinylated membrane proteins in nanodiscs using horse radish peroxidase (HRP)-conjugated anti-M13 monoclonal antibody (GE Healthcare, #27-9421-01). Assays were performed in library sorting buffer (200 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: The real time protein-protein interaction of protease and PDZ domain proteins of PfHtrA2was analysed by SPR using Biacore T200 instrument (GE Healthcare, Uppsala, Sweden) as described previously [7] ...
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bioRxiv - Immunology 2022Quote: ... The antibody and RBD affinity ability were estimated employing a 1:1 binding fit model in Biacore Evaluation software (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... were used for immunoblotting along with sheep anti-mouse-HRP NXA931V secondary antibody (GE Healthcare, 1:5,000 or 1:10,000, respectively). All yeast growth and Western blot data shown are representative of at least two biological replicates.
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated for 1 h at room temperature with secondary antibody (anti-mouse IgG, 1:10,000 in 3% BSA/TBS-T; GE Healthcare, #RPN4201) linked to horseradish peroxidase ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were washed three times with TBS-Tween 1 % and incubated for 1 h at room temperature with a secondary antibody anti-Mouse conjugated to horseradish peroxidase (GE Healthcare, NA9310 ...
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bioRxiv - Molecular Biology 2024Quote: ... and horseradish peroxidase-conjugated anti-rat (NA935V [1:1000]) and anti-mouse (NA931V [1:1000]) IgG antibodies (GE Healthcare, IL, USA). Signals were detected using WesternSure Premium Chemiluminescent Substrate (#926-95010 ...
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bioRxiv - Developmental Biology 2021Quote: ... Extracted proteins were immunoprecipitated with Protein A Sepharose CL-4B beads (GE Healthcare, USA) and desired primary antibodies ...
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bioRxiv - Cell Biology 2021Quote: Protein purification was carried out with the AKTA-prime protein purification system (GE Healthcare). First ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified from supernatants by protein A affinity chromatography using ÄKTA (GE Healthcare) or NGC (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... S1 -Fc proteins were purified using Protein A-Sepharose beads (GE Healthcare Life sciences) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Fc-tagged ACE2 protein was then purified with a Protein A column (GE Healthcare) followed by size exclusion chromatography.
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bioRxiv - Molecular Biology 2020Quote: ... coupled to protein G- and/or protein A-sepharose beads (GE Healthcare UK Ltd.). Proteins were eluted from beads with 0.2M glycine HCl pH2.5 ...
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bioRxiv - Microbiology 2020Quote: ... Fc-tagged ACE2 protein was then purified with a Protein A column (GE Healthcare) followed by size exclusion chromatography.
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bioRxiv - Immunology 2022Quote: ... purified from culture supernatants using the Protein A/Protein G GraviTrap kit (GE Healthcare), and buffer-exchanged into PBS as described (12).
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bioRxiv - Plant Biology 2023Quote: ... We purified recombinant proteins and MBP protein by a Hisprep IMAC column (GE Healthcare). Cis-fragments of target gene promoters (34-bp free probes of OsRFL containing the NRE motif and 23-bp free probes of OsMOC1 containing the WNNN (CCANTG ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysate was incubated with Protein G SepharoseTM 4 Fast Flow beads (GE Healthcare) for pre-clearance for 30 min at 4°C ...