Labshake search
Citations for GE Life Sciences :
451 - 500 of 1386 citations for 7 Chlorofuro 2 3 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: ... and subcloned into the pGEX 4T-2 vector (GE Healthcare Life Sciences) for expression in BL21 bacteria ...
-
bioRxiv - Biochemistry 2020Quote: ... pH 7.2 using a 2×5ml HiTrap desalting column (GE Life Sciences) and concentrated to 10 mg/mL in a centrifugal concentrator ...
-
bioRxiv - Biophysics 2022Quote: ... the supernatants were incubated with 2 mL glutathione-Sepharose beads (GE Healthcare) at 4°C for 3 hrs ...
-
bioRxiv - Biochemistry 2022Quote: ... 2 mM DTT) was passed through HisTrapTM FF (GE Healthcare Life Sciences) column ...
-
bioRxiv - Microbiology 2020Quote: ... with a Ni2+ HiTrap IMAC (2 × 1 ml) column (GE Healthcare, Poland) preequilibrated with the binding buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... and applied to tandem fast-flow Ni+2-NTA HisTrap (GE Healthcare) columns ...
-
bioRxiv - Immunology 2022Quote: ... 2 mM CaCl2 using a HiPrep 26/10 Desalting column (GE Healthcare) and subsequently treated with enterokinase overnight at room temperature to remove the strep-tag ...
-
bioRxiv - Molecular Biology 2019Quote: ... 2 mM TCEP) and applied to a 25 ml Source15Q (GE Healthcare) strong anion exchange column equilibrated in buffer A ...
-
bioRxiv - Microbiology 2021Quote: ... NEC220 was passed over 2 × 1 mL HiTrap Talon columns (GE Healthcare), rather than ion exchange ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The secondary antibody was incubated for 2 hours (Anti-rabbit, GE Healthcare, 1:10000 or Anti-mice ...
-
bioRxiv - Biophysics 2022Quote: ... The supernatant was incubated with 2 mL Ni-NTA resin (GE Healthcare), equilibrated with buffer A (50 mM Tris-HCl ...
-
bioRxiv - Biophysics 2022Quote: ... RMI1/2 was further purified using a Superdex 200 Increase (GE Healthcare) in buffer I ...
-
bioRxiv - Biochemistry 2022Quote: ... 2 mM TCEP) and applied to a 25 ml Source15Q (GE Healthcare) strong anion exchange column equilibrated in buffer A ...
-
bioRxiv - Cell Biology 2022Quote: ... Images for Figure 2 were obtained using a DeltaVision Elite (Applied Precision) maintained at 37°C and 5% CO2 equipped with a 40x and 63x PLANApo S lens (Olympus ...
-
bioRxiv - Immunology 2023Quote: ... C4BPα1-2 was applied to a HiTrap Q HP column (GE Healthcare) and eluted using a 0-1 M NaCl gradient in 50 mM Tris ...
-
bioRxiv - Biophysics 2021Quote: ... 4°C for 30 minutes and then loaded onto a 5mL NiNTA affinity column (HisTrap HP, GE Healthcare) pre-equilibrated with the lysis buffer ...
-
bioRxiv - Molecular Biology 2021Quote: ... C-terminal FLAG-tagged library of 65 chaperones was prepared from the ORFeome library (purchased from GE Healthcare), and transferred from pDONR223 into pcDNA3.1-ccdb-FLAG using gateway cloning ...
-
bioRxiv - Microbiology 2020Quote: ... BHK-21 cells were grown at 37 °C with 5% CO2 in DMEM (HyClone, GE Healthcare life sciences) supplemented with 10% bovine serum and 1 U/mL penicillin/streptomycin ...
-
bioRxiv - Biochemistry 2019Quote: ... 61236 × g for 20 min at 4 °C) 4 ml of Glutathione Sepharose 4B bead suspension (GE Healthcare) were added to the clarified lysate and incubated for 20 min at 4°C under constant shaking ...
-
bioRxiv - Molecular Biology 2019Quote: ... GST-fused IQGAP1-N and IQGAP1-C were purified using Glutathione Sepharose 4B (17-0756-01; GE Healthcare). Next ...
-
bioRxiv - Microbiology 2021Quote: ... 4 °C) and the supernatant (clear lysate) was then loaded onto GST GraviTrap columns (GE Healthcare Life Sciences) that had been pre-washed with PBS and buffer B (PBS pH 7.4 ...
-
bioRxiv - Microbiology 2021Quote: ... These were stored in a chilled compartment at 9°C within a skirted 384 well plate (GE Healthcare) before being washed over the chip surface in a buffer containing 20 mM HEPES (pH 7.5) ...
-
bioRxiv - Biochemistry 2020Quote: ... which utilized the encoded C-terminal hexa-histidine tag (HisTrap™ Ni2+-NTA column; GE Healthcare Life Sciences), and size-exclusion chromatography (Superdex S20010/300 column ...
-
bioRxiv - Cell Biology 2021Quote: ... Gene-edited NIH3T3 cells were cultured at 37°C in 5% CO2 in DMEM (GE Healthcare, Chicago, IL) containing 10% FBS ...
-
bioRxiv - Biochemistry 2021Quote: ... 4°C) the sample was applied onto a HiLoad Superdex 200 (16/60) column (GE Healthcare, now Cytiva). Separation was performed at a flow rate of 0.8 mL/min in SEC buffer (50 mM Hepes pH 8.0 ...
-
bioRxiv - Biochemistry 2020Quote: All SPR measurements were performed at 25° C using a Biacore 3000 instrument (BIAcore, GE Healthcare, Piscataway, NJ) as described previous24,25 ...
-
bioRxiv - Microbiology 2021Quote: ... The supernatant was maintained at 4°C for protein purification with a prepared HisTrap HP column (GE Healthcare). Purified protein samples were concentrated using an Amicon 4 centrifugal filtration tube centrifuged for 5 min at 3600 rcf (4 °C) ...
-
bioRxiv - Cell Biology 2021Quote: ... the lysates were incubated 16 hours at 4°C with Glutathione Sepharose 4B (GE Healthcare, GE17-0756-01). Next ...
-
bioRxiv - Biochemistry 2022Quote: ... The resulting supernatant was incubated for 4-5 h at 4 °C on glutathione sepharose 4B (GE Healthcare) (3 mL per 2 L expression) ...
-
bioRxiv - Immunology 2022Quote: ... 4°C) and mononuclear cells isolated by density gradient centrifugation on a discontinuous 35%/70% Percoll (GE Healthcare) gradient (2000 rpm [931 g] ...
-
bioRxiv - Immunology 2022Quote: The hcAb-binding properties of B10 were analyzed at 25 °C on a Biacore T200 instrument (GE Healthcare) using 10 mM HEPES pH 7.4 ...
-
bioRxiv - Microbiology 2021Quote: ... Samples were then separated in 10% SDS polyacrylamide gels and transferred to Hybond C nitrocellulose membranes (GE Healthcare). After blocking with 5% non-fat milk in PBS/0.05% Tween ...
-
bioRxiv - Biochemistry 2021Quote: ... 4 °C) and the supernatant applied to a GSTrap 5 ml FF column affinity purification column (GE Healthcare) at 4 °C equilibrated with Tris-Cas-A buffer ...
-
bioRxiv - Cell Biology 2022Quote: All chromatography steps were performed at 4°C using ÄKTAxpress or ÄKTA pure protein purification systems (GE Healthcare). Firstly ...
-
bioRxiv - Biochemistry 2019Quote: Removal of the His6-tag (Rab8a) was performed by overnight incubation at 4 °C with thrombin (GE Healthcare), followed by a second Ni2+-agarose column ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were grown at 37°C and 5% CO2 in high-glucose (4.5 g/L) DMEM (GE Healthcare) supplemented with 10% fetal calf serum (Biochrom ...
-
bioRxiv - Plant Biology 2022Quote: ... 4°C for 30min and used for the purification of HIS-AgLTP24 using His GravityTrap columns (GE Healthcare) with modified binding (100mM Tris ...
-
bioRxiv - Immunology 2021Quote: All surface plasmon resonance experiments were performed at 25°C using a Biacore T200 instrument (Cytiva, GE Healthcare) equipped with a flat carboxymethyldextran sensor chip (CMDP ...
-
bioRxiv - Cell Biology 2021Quote: ... The experiments were performed at 25°C using a BiacoreTM T200 instrument (GE Healthcare Life Sciences, Uppsala, Sweden) flowed with EP+ buffer (10mM HEPES ...
-
bioRxiv - Biochemistry 2020Quote: ... FIH was induced for 16 h at 16°C and purified using a MPBTrap HP column (GE Healthcare). Proteins were exchanged into 150 mM NaCl ...
-
bioRxiv - Biochemistry 2020Quote: ... CAD was induced for 8 h at 30°C and purified using a HisTrap HP column (GE Healthcare). FIH was induced for 16 h at 16°C and purified using a MPBTrap HP column (GE Healthcare) ...
-
bioRxiv - Microbiology 2022Quote: ... protein samples were run on 12-15% polyacrylamide gels and transferred to nitrocellulose Hybond™-C (GE Healthcare) membranes via Bio-Rad Trans-blot Turbo transfer machine ...
-
bioRxiv - Biochemistry 2022Quote: ... followed by size-exclusion chromatography at 4 °C on a HiLoad Superdex 200 pg (GE Healthcare Life Sciences) column pre-equilibrated in the same buffer ...
-
bioRxiv - Microbiology 2022Quote: ... The supernatant was collected and incubated with GST beads for 1h at 4°C on rotator (GE Healthcare). GST beads were washed three times with phosphate buffered saline (PBS) ...
-
bioRxiv - Neuroscience 2022Quote: ... incubated overnight at 4°C and subjected to size exclusion chromatography using Superdex 200 16/60 (GE Healthcare) in 20 mM Tris-HCl (pH 7.5 ...
-
bioRxiv - Cell Biology 2023Quote: ... Full-length Nedd4L (N-terminal GST-tagged, C-terminal His6-tagged) was purified by glutathione– Sepharose (GE Healthcare) column chromatography ...
-
bioRxiv - Biochemistry 2022Quote: Ca2+ binding was characterized by ITC at 25 °C using a VP-ITC microcalorimeter (GE Healthcare, Northampton, MA) with a cell volume of 1.4619 ml in Na+ or K+ containing buffers (20 mM Tris pH 7.9 ...
-
bioRxiv - Microbiology 2023Quote: The surface plasmon resonance (SPR) experiments were performed at 25 °C on a BIAcore 2000 instrument (GE Healthcare). DsrC-trisulfide was immobilized on a NTA sensor chip (GE Healthcare ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... then stored at −20°C before purification by fast protein liquid chromatography (FPLC) (AKTA Purifier; GE Healthcare, UK) using a pre-packed HiLoad Superdex 30 26/600 column equipped with a UV detector ...
-
bioRxiv - Cell Biology 2024Quote: ... Live imaging was carried out in a chamber at 37°C using a Deltavision Core (Applied Precision™) microscope equipped with a CoolSnap HQ2 (Photometrics ...