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Citations for GE Life Sciences :
451 - 500 of 728 citations for 3 2 Methylphenoxy methyl piperidine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 300 µg of the prepared lysate was incubated with the appropriate antibody (3 µg) and pulled down using protein A Sepharose 6MB (GE healthcare # 17-0469-01). All primary antibodies used in this study are listed in table T3 ...
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bioRxiv - Neuroscience 2021Quote: ... 1 mm × 1 mm × 1 mm) and brain FDG-PET were acquired simultaneously on a hybrid 3 T SIGNA PET-MR scanner (GE Healthcare, Milwaukee, Wisconsin, USA) at the CUB Hôpital Erasme (Brussels ...
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bioRxiv - Neuroscience 2021Quote: Whole-brain images will be acquired at the Lane Neuroimaging Laboratory at the University of Wisconsin–Madison HealthEmotions Research Institute (Department of Psychiatry) using a 3 Tesla GE MR scanner (Discovery MR750; GE Healthcare, Chicago, IL, USA). A high-resolution T1 image will be acquired with a fast spoiled gradient recalled echo sequence (8.2 ms TR ...
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bioRxiv - Immunology 2021Quote: ... Cells were resuspended in 10 ml FACS wash and filtered through a nylon filter before being overlayed onto 3 ml Ficoll (GE Healthcare, 17-1440-03). Cell/Ficoll mixture was centrifuged at 931g for 1 hour at room temperature with brakes off ...
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bioRxiv - Neuroscience 2024Quote: ... Blots were rinsed with TBS containing 0.1% Tween 20 (3 times for 15 min under shaking at RT) and probed with an anti-rabbit HRP conjugated antibody (NA934V, GE healthcare, Piscataway, NJ, USA) for 2 h at RT ...
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bioRxiv - Plant Biology 2022Quote: ... 3 mL of the centrifuged supernatant was applied to a PD-10 column filled with Sephadex G-25 resin (GE Healthcare, Chicago, IL, USA) equilibrated with the homogenizing buffer ...
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bioRxiv - Immunology 2024Quote: ... The cultures were started in a volume of 400 mL of basal medium at a cell density of 350,000-400,000 cells/mL and were supplemented with Cell Boost 7a (Hyclone) at 3% v/v and Cell Boost 7b (Hyclone, GE Healthcare, Chicago, IL, USA) at 0.3% v/v on days 0 ...
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bioRxiv - Genetics 2021Quote: ... DNA was transferred in 2 x SSC to positively charged nylon membranes (GE Healthcare Amersham Hybond-N+) and was then immobilized by ultraviolet cross-linking (1200 J) ...
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bioRxiv - Molecular Biology 2020Quote: ... 500 μL lysate (2 mg protein) was injected through a 0.75mm loop (GE Healthcare 18-1112-53). Run parameters were 0.5 mL/min and 1.5MPa for 1 column volume ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 mM DTT prior to injection onto a Superdex 200 10/300 GL column (GE Healthcare). PTEN and P-Rex2 alone at corresponding concentrations demonstrate the peak-shift upon complex formation.
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bioRxiv - Immunology 2021Quote: ... Affinity-purified SARS-CoV-2 RBD-SD1 was further purified over a Superdex75 column (GE Life Sciences). SARS-CoV-2 S HP ...
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bioRxiv - Genomics 2020Quote: ... The oligonucleotide pool was purified using 2 x illustra NAP-5 Columns (GE Life Sciences, PN: 17085302). The resulting solution was evaporated to dryness ...
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bioRxiv - Genetics 2022Quote: ... and the mouse heart was imaged with a 13-MHz probe using 2-dimensional echocardiography (GE Healthcare). All measurements were made by an independent operator to whom the study groups were masked.
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bioRxiv - Immunology 2022Quote: ... GPC-I53-50A was concentrated to at least 2 µg/ml using Vivaspin centrifugal filters (GE Healthcare). To generate complexes ...
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bioRxiv - Cell Biology 2020Quote: ... and 2 μL (24-well plates) or 0.6 μl (96-well plates) Dharmafect-1 (GE Life Sciences) in serum-free Minimum Essential Medium Eagle (MEM ...
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bioRxiv - Immunology 2020Quote: ... Affinity-purified SARS-CoV-2 RBD-SD1 was further purified over a Superdex75 column (GE Life Sciences). SARS-CoV-2 S-2P and SARS-CoV-2 HexaPro S were purified over a Superose6 Increase column (GE Life Sciences).
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bioRxiv - Molecular Biology 2021Quote: ... Supernatant was incubated (2 h, 4 °C) with 4.0 mL of Glutathione Sepharose 4B resin (GE Healthcare) that had been pre-equilibrated in the same buffer ...
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bioRxiv - Zoology 2020Quote: ... and 3500 V/2 mA/5 W for 6 h using a Multiphor II system (GE Healthcare). The IEF strip was then equilibrated for 15 min in 5 ml equilibration stock solution (6 M urea ...
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bioRxiv - Microbiology 2020Quote: ... Test media used was MEM supplemented with 2% fetal bovine serum (FBS, GE Healthcare Hyclone, Marlborough, MA) and 50 μg/mL gentamicin (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... Affinity-purified SARS-CoV-2 RBD-SD1 was further purified over a Superdex75 column (GE Life Sciences). MERS-CoV S1 was purified over a Superdex200 Increase column (GE Life Sciences) ...
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bioRxiv - Plant Biology 2022Quote: ... Gels were dried at 80 °C for 2 hrs and covered with phosphor imaging plate (GE Healthcare) overnight after RNA fixation in RNA fixation buffer (40% ethanol ...
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bioRxiv - Molecular Biology 2024Quote: ... coding sequences of AtCFI25a and AtCFI59 were cloned into pGEX5X-2 (GST) (Merck/GE Healthcare, Darmstadt, Germany) or pET30c (HIS ...
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bioRxiv - Cell Biology 2023Quote: ... 100-250 mM NaCl and 2 mM DTT using the ÄKTA™ Pure 25 system (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... for 2 hours at room temperature and visualisation using enhanced chemiluminescence (ECL kit, GE Healthcare Life Sciences). Data were analysed using the gel analysis package in FIJI.
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bioRxiv - Molecular Biology 2024Quote: ... 2 μL of total RNA was dropped onto an Amersham Hybond-N+ membrane (GE Healthcare, cat# RPN203B), then the membrane was briefly air dried and crosslinked using Stratalinker 2400 UV Crosslinker twice using Autocrosslink mode ...
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bioRxiv - Neuroscience 2024Quote: ... PCR products were analyzed on 2% agarose gels and imaged using an Amersham Imager 680 (GE Healthcare).
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bioRxiv - Developmental Biology 2021Quote: Single cell suspension was collected by centrifugation (3 min at 300 g) and total RNA was extracted by Illustra™ RNAspin RNA Isolation Kit (GE Healthcare GE25-0500-72) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The probe was radioactively labeled with 3 µl 32P-[γ]-ATP by a polynucleotide Kinase reaction and cleaned up using a MicroSpin Sephadex G-50 column (GE Healthcare, #GE27-5330-01). The labeled oligonucleotide was denatured at 95°C for 10 min and mixed with 20 ml fresh hybridization buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... at a 1:1000 dilution in 3% milk overnight at 4°C before incubation with secondary HRP-linked sheep anti mouse (NA931-1ML, GE Healthcare Life Sciences, Buckinghamshire, UK) at a 1:3000 dilution in 5% milk for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... 1 mm x 1 mm x 1 mm) and FDG-PET data acquisitions were performed simultaneously on a hybrid 3 T SIGNA™ PET-MR scanner (GE Healthcare, Milwaukee, Wisconsin, USA) at the Service of Nuclear Medicine (CUB Hôpital Erasme ...
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bioRxiv - Plant Biology 2022Quote: ... chromosomal DNA was purified and amplified to 5.4 - 7.9 μg by multiple displacement amplification (Supplementary Table 3) using an Illustra GenomiPhi V2 DNA Amplification Kit (GE Healthcare, Chalfont St. Giles, United Kingdom) as described by Šimková et al ...
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bioRxiv - Biophysics 2021Quote: ... Polyacrylamide gels were polymerized between two glass coverslips treated with 2% dimethyldichlorosilane (Plus One Repel Silane, GE Healthcare). For 0.6 kPa gels ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM DTT at a flow rate of 2 ml/min using an Akta purifier system (GE Healthcare), eluting at approximately 100 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... were subjected to the short column with 2 ml Sephacryl S-500 resin (GE Healthcare Life Sciences, IL) equilibrated with PBS ...
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bioRxiv - Biochemistry 2021Quote: ... dried for 2 h at 65°C and radiolabelled products visualised using a Typhoon FLA-700 (GE Healthcare) following exposure to a phosphorimaging plate for 24-72 h.
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bioRxiv - Microbiology 2020Quote: ... concentrated to 2 mg/ml by spin filtration and then run on a Superdex 200 column (GE Healthcare). MALS was conducted using a MiniDAWN and Optilab system (Wyatt Technologies) ...
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bioRxiv - Biophysics 2021Quote: ... the sample was loaded at 2 ml min−1 onto a 5 ml HisTrap HP column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was mixed with 2 ml of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) equilibrated in Dynein-lysis buffer and incubated for 3-4 h with rotation along the long axis of the tube ...
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bioRxiv - Biophysics 2022Quote: ... were further subjected to Ni-NTA purification by mixing with 2 mL of Ni-Sepharose resin (GE Healthcare) for 4 hrs and the bound proteins were eluted with a gradient of 100-500 mM imidazole in 10 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... were further subjected to Ni-NTA purification by mixing with 2 mL of Ni-Sepharose resin (GE Healthcare) for 4 hrs ...
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bioRxiv - Biochemistry 2020Quote: ... 2 mM DTE (IbpA) or applied to a HiLoad™ 16/600 Superdex™ 200pg column (GE Healthcare) in 20 mM Hepes pH 7.4 ...
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bioRxiv - Developmental Biology 2021Quote: For mass spectrometry the beads were incubated with 150 µL the elution buffer (GE Healthcare, 2-1042-025) for 10 min at 4 °C while rotating ...
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bioRxiv - Molecular Biology 2022Quote: ... 93-207 Bcl-2) with a C-terminal 6His tag and purified using Ni2+ IMAC (GE Healthcare, Fastflow). Similarly ...
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bioRxiv - Immunology 2022Quote: The binding kinetics of nanobodies to SARS-CoV-2 RBD were analyzed using SPR (Biacore 8K; GE Healthcare). The nanobodies were captured by the ProA sensor chip and serial dilutions of SARS-CoV-2 RBD were then flowed through at a rate of 30 μL/min in PBS buffer (with 0.05% Tween20 ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was applied onto a self-packaged GST-affinity column (2 mL Glutathione Sepharose 4B; GE Healthcare) and contaminant proteins were removed with wash buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Synthetic Biology 2020Quote: ... the plate was blocked with 200 μl of a 2% (w/v) ECL Advance Blocking Reagent (GE Healthcare) solution in PBS-T (PBS supplemented with 0.1% (v/v ...
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bioRxiv - Genomics 2021Quote: ... 2 mM MgCl2) and then loaded onto two tandem 5 mL heparin HP pre-packed columns (GE Healthcare). Sen1 was eluted using a linear gradient from 20% to 100% of buffer B2 containing 50 mM HEPES-NaOH ...
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bioRxiv - Microbiology 2020Quote: ... The NEC220 and NEC-CBM constructs were passed over 2 x 1 mL HiTrap Talon columns (GE Healthcare), rather than ion exchange as previously described ...
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bioRxiv - Microbiology 2023Quote: ... then 2-D gel samples were collected by scraping cells in 250 ul of 2-D rehydration buffer (4% CHAPS, 8 M urea, 0.5% IPG Buffer (pH 3.0-10.0; GE Healthcare), 0.002% Bromophenol Blue ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were developed with ECL 2 western blot substrate and scanned with an ImageQuant LAS 4000 (GE Healthcare), and signals analyzed with ImageQuant TL 7.0 software (GE Healthcare).