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Citations for GE Life Sciences :
451 - 500 of 541 citations for 2 Pyridin 2 yl acetic acid d4 hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The sample was concentrated to 2-3 mL for separation on exclusion size chromatography (HiLoad 16/600 SuperdexTM 75pg, GE Healthcare; ÄKTA Avant). Finally ...
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bioRxiv - Biophysics 2023Quote: ... size exclu-sion chromatography was conducted by injecting 2-5 mL sample onto HiLoad 16/600 Superdex 200 Column (GE Healthcare Life Sciences) connected to a BioRad NGC Quest 10 FPLC system ...
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bioRxiv - Biochemistry 2023Quote: ... was then incubated for 1 hour at 4°C on a rotating platform with 2 mL Sepharose 2B resin (GE Healthcare Life Sciences) suspended in 2 mL kinase buffer (50 mM Tris ...
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bioRxiv - Immunology 2024Quote: ... IgG were purified from cell culture supernatant 6-7 days after transfection via Protein A affinity chromatography using 2 mL or 5 mL column HiTrap MabSelect SuRe Protein A column (GE Healthcare, Life Sciences) dependent on culture volume ...
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bioRxiv - Molecular Biology 2024Quote: ... Reactions were incubated for 2 h at 25°C and then filtered through a Protran nitrocellulose membrane (Whatman, GE Healthcare Bioscience, Pittsburgh, PA) on top of a Hybond-XL (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: ... was used according to manufacturer’s guideline to clone cDNAs into pUASTattB (kindly provided by K. Basler, University of Zurich) and pGEX-6P-2 (GE Healthcare Life Sciences). Full length (FL ...
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bioRxiv - Biochemistry 2024Quote: ... The diluted peak fractions were loaded at 2 mL/min in Buffer A onto a 5 mL HiTrap Heparin column (GE Healthcare Life Sciences) and eluted at 2 mL/min with a 50 mL gradient from 30% to 100% Buffer B ...
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bioRxiv - Biochemistry 2020Quote: ... Nickel-nitrilotriacetic acid (Ni-NTA) resin was purchased from GE Healthcare. The plasmid encoded an N-terminal 8X His-SUMO-tagged hNatD in the pET-21a vector was obtained from Genscript ...
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bioRxiv - Cell Biology 2021Quote: ... FBS and MEM Non-essential amino acids (NEAA) from GE Healthcare Life Sciences (Logan ...
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bioRxiv - Cell Biology 2022Quote: ... and 1x non-essential amino acids (GE Healthcare, Pittsburgh, PA, USA). 3T3-L1 cell lines with PTRF/Cavin-1 inactivated by CRISPR (K5 and K3 ...
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bioRxiv - Cell Biology 2020Quote: ... The mixture was incubated for 2 hours at RT and antibodies were isolated using illustra™ NAP-5 columns (GE Healthcare Life Sciences™), resulting in an average labeling rate between 0.8 and 1.
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bioRxiv - Microbiology 2021Quote: ... PCR elongation was performed at 72 °C (2 min per kb) and the amplified products were purified by illustra GFX purification kit (GE Healthcare Little Chalfont, UK).
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bioRxiv - Biochemistry 2020Quote: ... The resulting lysate was loaded at a rate of 2 mL/min onto a prepacked 5 mL HiTrapFF Ni-Sepharose column (GE Healthcare Life Sciences, MA) that had been equilibrated with Buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting lysate was loaded at a rate of 2 mL/min onto a prepacked 5 mL HiTrapFF Ni-Sepharose column (GE Healthcare Life Sciences, MA) that had been equilibrated in Buffer B ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein were separated on 4-15 % Tris-glycine (TG) gels (#45101080003-2, SMOBIO, Hsinchu, Taiwan) and electro-transferred onto polyvinylidene fluoride (PVDF) membranes (#A16646282, GE Healthcare, Chicago, IL, USA) in TG buffer ...
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bioRxiv - Immunology 2021Quote: ... The blots were washed in TBS-T and incubated in secondary antibody in antibody buffer for 2 hours before washing again in TBS-T before visualising with ECL (GE Healthcare, Chicago, IL, USA) and imaging in an ALLIANCE 6.7 Chemiluminescence Imaging System (UVITEC ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The secondary antibody was incubated for 2 hours (Anti-rabbit, GE Healthcare, 1:10000 or Anti-mice, GE Healthcare, 1:10000, Chicago, Illinois, USA). The bands were detected using chemiluminescence methods (ECLPrime® ...
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bioRxiv - Biochemistry 2022Quote: ... was passed through the HisTrap column previously equilibrated with IMAC buffer A at 2 ml/min flowrate with a peristaltic pump (GE Healthcare Pump P-1) and then washed with 5 CVs of buffer IMAC A supplemented with 50 mM imidazole ...
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bioRxiv - Microbiology 2022Quote: ... were mixed together at a molar ratio of 1:2 and loaded on a HiLoad 16/600 Superdex 200 prep grade column (GE Healthcare, Chicago, IL, USA) pre-equilibrated with 20mM Tris-HCl (8.0) ...
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bioRxiv - Biophysics 2023Quote: ... The cleared lysate was supplemented with CaCl2 to a final concentration of 2 mM and then incubated for 1 h at 4 °C with washed Sepharose 4B Calmodulin beads (GE Healthcare #17-0529-01) in a spinning rotor ...
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bioRxiv - Evolutionary Biology 2023Quote: ... following the manufacturer’s protocol using 15 µl of PCR product and 2 µl Streptavidin Sepharose High Performance beads (GE Healthcare, cat. GE17-5113-01).
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bioRxiv - Molecular Biology 2023Quote: ... 300 μM 5’ thiol-modified 18-nt DNA oligonucleotides—comprised of an AAA base sequence and a 15-base secondary probe binding sequence (Eurofins)—were reduced with 50 mM dithiothreitol in PBS at room temperature for 2 hours and purified using NAP5 columns (GE Healthcare 17-0853-01). Maleimide-activated antibodies were then combined with 11 equivalents of the reduced form of the thiol-modified DNA oligonucleotides in PBS and incubated at 4℃ overnight ...
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bioRxiv - Immunology 2024Quote: ... to 2 ml and subsequently loaded on a Sepharose 4 Fast Flow resin column (10 mm x 300 mm; GE Healthcare, Little Chalfont, UK), connected to the ÄKTA pure system (GE Healthcare ...
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bioRxiv - Immunology 2024Quote: ... Blood was drawn into BD Vacutainer Sodium-Heparin coated tubes and processed within 2 hours by Ficoll density centrifugation (GE Healthcare #17-1440-03).
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bioRxiv - Neuroscience 2021Quote: ... The slides were dried for 2-3 hours at 30°C and exposed to a Tritium Phosphor Screen (GE Healthcare, Fuji BAS-TR 2025 E) together with calibrated tritium standards (American Radiolabeled Chemicals ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was then filtered through a 0.2 µm syringe filter before being loaded onto 2 x 5 mL StrepTrap HP Columns (GE Healthcare Life Sciences 28-9075-47), equilibrated in Step A Buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was aspirated to the 5 mL mark and the samples were transferred to fresh 15 mL tubes containing 2 mL of 100% Percoll (GE Healthcare catalog no. 17-0891-01). Water was added to the 10 mL mark ...
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bioRxiv - Cell Biology 2020Quote: ... were activated by 100 mM DTT for 2 hours at room temperature in the dark and then purified using NAP5 columns (GE Healthcare Life Sciences, 17-0853-02) to remove excessive DTT ...
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bioRxiv - Biochemistry 2020Quote: Microsomal protein (2 µg) was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose blotting membranes (GE Healthcare Life Sciences, Little Chalfont, UK). The membranes were blocked with 5% skim milk (BD Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... and photosynthetic pigments via high-performance liquid chromatography (HPLC; 2 L) were collected on 25-mm diameter GF/F glass microfiber filters (Whatman, GE Healthcare Life Sciences, Chicago, IL, USA) and stored in aluminum foil at −80°C until extraction ...
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bioRxiv - Immunology 2021Quote: ... and proteins purified from culture supernatants Ni-nitriloacetic acid (NTA) resin (GE HealthCare).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The recombinant protein was purified through a nickel-nitrilotriacetic acid column (GE Healthcare) and subsequently loaded on a gel filtration column Sephacryl S-200 HR (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: ... the supernatant was either loaded onto a nickel-nitrilotriacetic acid column (GE Healthcare) or incubated with nickel-nitrilotriacetic acid resin (Qiagen) ...
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bioRxiv - Molecular Biology 2021Quote: ... purified with Ni-nitrilotriacetic acid (NTA) affinity chromatography (HisTrap HP columns from GE Healthcare) in an AKTA purifier (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Overnight trichloroacetic acid/acetone precipitation was performed with 2D Clean-Up kits (GE Healthcare) as instructed by the manufacturer ...
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bioRxiv - Biochemistry 2022Quote: ... MBNL1 (amino acids 1-269 of MBNL140) was obtained in pGEX-6P1 (GE Healthcare). Plasmids were transformed into E ...
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bioRxiv - Immunology 2020Quote: ... The protein was first purified with a Ni2+-nitrilotriacetic acid (NTA) resin (GE Healthcare,) using an elution buffer consisting of 50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... affinity chromatography with Ni-NTA column (Nickel-nitrilotriacetic acid, 5 ml HisTrap, GE Healthcare) was performed using FPLC (Äkta explorer ...
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bioRxiv - Biochemistry 2021Quote: ... a modified trichloroacetic acid–acetone precipitation method (Clean-Up Kit; GE Healthcare, Munich, Germany) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... affinity chromatography with Ni-NTA column (Nickel-nitrilotriacetic acid, 5 ml HisTrap, GE Healthcare) was performed using FPLC (Äkta explorer ...
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bioRxiv - Microbiology 2023Quote: ... Radioactively labelled proteins or nucleic acids were visualised using storage phosphor screens (GE Healthcare) and a Typhoon 9400 imager (GE Healthcare).
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bioRxiv - Genomics 2020Quote: ... The His6-tagged fusion protein was purified with a Ni2+-nitrilotriacetic acid column (GE Healthcare). The EtHAS B protein ...
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bioRxiv - Biochemistry 2020Quote: ... the protein supernatant was loaded onto a nickel-nitrilotriacetic acid (Ni-NTA) column (GE Healthcare) containing 0.1 M Ni2+ and equilibrated with the same loading buffer ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was purified on a Ni2+-nitrilotriacetic acid (Ni-NTA) sepharose resin (GE Healthcare), eluting with a linear gradient of imidazole (100 – 400 mM ...
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bioRxiv - Biochemistry 2022Quote: ... onto a pre-equilibrated 5 mL nickel nitrilotriacetic acid affinity column (HisTrap HP, GE Healthcare) with a running buffer (20 mM Tris buffer at pH 7.5 containing 200 mM NaCl and 30 mM imidazole) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Each protein was purified on a 5ml nickel nitrilotriacetic acid (Ni2+-NTA) resin (GE Healthcare). The column was washed stepwise with 20 ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was then applied to Ni2+-NTA (Nickel-Nitrilotriacetic acid) sepharose column (GE Healthcare, USA) pre-equilibrated with the lysis buffer at a flow rate of 0.2 ml/min ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was purified using nucleic acid binding plates (96-well, 800 μl UNIFILTER Microplate, GE Healthcare) and was stored with RNAse-inhibitor at −80°C.
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bioRxiv - Molecular Biology 2020Quote: ... The human LRFN4 (last 40 amino acids) was cloned from LRFN4 cDNA (MHS6278-202832013, GE Healthcare) and the human LRFN5 (last 40 amino acids ...
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bioRxiv - Microbiology 2021Quote: ... Nucleic acid (400 ng) was spotted on to Amersham Hybond N+ nylon membrane (GE Healthcare Lifesciences), air-dried and UV cross-linked for 3 min using a transilluminator ...