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Citations for GE Life Sciences :
451 - 500 of 2431 citations for 1 Benzyl 3 Tert Butyldimethylsilyl Oxy Methyl Piperidin 4 One since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... which was underlayed with 4 ml 80% percoll solution (GE Healthcare-Life Sciences, Chicago, IL, USA). Tubes were centrifuged (1600 G ...
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bioRxiv - Molecular Biology 2021Quote: ... Supernatant was purified using Protein G Sepharose 4 Fast flow (GE Healthcare Life Sciences, Pittsburgh, PA) as per manufacturer protocol.
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bioRxiv - Immunology 2020Quote: ... Cell suspension was then pelleted and then re-suspended in 4 mL 40% Percoll (GE Healthcare), layered on 4 mL 70% Percoll and centrifuged at 800xg (RT ...
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bioRxiv - Biochemistry 2022Quote: ... The in-house resin is prepared by coupling N-hydroxysuccinimide (NHS)-Activated Sepharose 4 (GE Healthcare) with 2-(Pyridyldithio ...
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bioRxiv - Biochemistry 2023Quote: ... 4°C) and the supernatant incubated for 1.5 h with IgG-coupled sepharose beads (GE Healthcare). The beads were washed with lysis buffer and proteins eluted by cleavage with TEV protease ...
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bioRxiv - Pathology 2023Quote: ... 4° C) and purified via anion-exchange chromatography with an AKTA pure chromatography system (GE Healthcare). The sample was passed through an anion-exchange column (HiTrap Q HP ...
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bioRxiv - Microbiology 2024Quote: ... and the surface proteins were bound to streptavidin Sepharose beads overnight at 4°C (GE Healthcare). Beads were then washed with buffer containing 100 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C and HRP-conjugated goat anti-mouse IgG (0.2 μg/mL; GE Healthcare) for 2hr at RT ...
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bioRxiv - Plant Biology 2023Quote: ... Supernatants were incubated for 2 h with Glutathione Sepharose® 4 Fast Flow Beads (GE Healthcare) while rotating ...
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bioRxiv - Microbiology 2022Quote: ... 4°C) and the supernatant was passed over a 5 mL HisTrap FF column (GE Healthcare) by an ÄKTApurifier system (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... 4°C) and the supernatant was passed over a 5 mL StrepTrap HP column (GE Healthcare) by an ÄKTApurifier system (GE Healthcare) ...
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bioRxiv - Molecular Biology 2024Quote: ... or in pGEX-4 T2 plasmid containing an N-terminal GST tag (GE Healthcare/Cytiva; cytivalifesciences.com) have been described previously 71 ...
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A resource for extracellular vesicles from activated CD4 T cells that relay pro-inflammatory signalsbioRxiv - Immunology 2024Quote: ... loaded to Sepharose 4 Fast Flow affinity resin column (10 mm x 300 mm; GE Healthcare) mounted to ÄKTA pure system (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... The protein extract was precleared by incubation with nProtein A-Sepharose 4 Fast Flow (GE Healthcare) for 30 min and then incubated with 20 uL of rabbit anti-Myc antibody (ab9106 ...
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bioRxiv - Biophysics 2024Quote: ... for 45 min at 4 °C and loaded on an empty PD-10 column (GE Healthcare). The column was washed with 15 column volumes of wash buffer containing 20 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were then washed 3 times in TBS-T and bands visualized with Amersham ECL chemiluminescence reagent (GE Healthcare). Blots were stripped (62.5 mM Tris-HCl pH 6.5 ...
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bioRxiv - Bioengineering 2020Quote: ... with HBS-EP+ as running buffer (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% v/v Surfactant P20, GE Healthcare). Ligands were immobilized on a CM5 chip (GE Healthcare # 29104988 ...
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bioRxiv - Biochemistry 2021Quote: ... flow rate 0.8 ml/min) or Superdex 200 Increase 5/150 (~3 ml, flow rate 0.45 ml/min) columns (GE Healthcare) were used ...
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bioRxiv - Cell Biology 2021Quote: ... SidK1-278-3×FLAG was further purified with a Superdex 200 10/300 Increase gel filtration column (GE Healthcare) equilibrated with 50 mM Tris-HCl pH 7.4 and 150 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... Samples were concentrated L using a 3 kDa Vivaspin™ 20 filter column (GE Healthcare, Mississauga, ON, μ Canada). The conformational mixtures were purified via size exclusion chromatography (SEC ...
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bioRxiv - Biophysics 2021Quote: ... 3 mM CaCl2 and 0.02% DDM) was injected onto a Superdex 200 Increase 10/300 GL column (GE Healthcare), equilibrated with buffer (25 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: Full-length mouse Csk (WT, H21I, K43D, and K222R3) were subcloned into a pGEX-6P-3 vector (GE Healthcare) containing an N-terminal glutathione S-transferase (GST ...
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bioRxiv - Microbiology 2021Quote: ... or denaturing 0.8% agarose gel for large RNA separation (0.2 to 3 kb) and transferred onto membrane Hybond N+ (GE Healthcare). RNAs were probed with γ32P 5’ end-labeled oligonucleotide (Table S3 ...
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bioRxiv - Neuroscience 2020Quote: Resting-state fMRI data were collected on a whole-body 3 Tesla MR750 MRI scanner (GE Healthcare, Milwaukee, WI) with an 8-channel receive-only head array coil at the Laureate Institute of Brain Research ...
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bioRxiv - Bioengineering 2020Quote: ... at room temperature with HBS-EP+ running buffer (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% v/v Surfactant P20, GE Healthcare). IgGs were immobilized on CM5 chips (GE Healthcare # 29104988 ...
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bioRxiv - Cell Biology 2024Quote: Col1a1-H2B-GFP;Rosa26-M2-rtTA;Slc23a2FL/FL;Mx1-Cre mice and Col1a1-H2B-GFP;Rosa26-M2-rtTA;Slc23a2FL/FL littermate controls were given 3 intraperitoneal injections of 40µg of poly I:C (GE Healthcare) dissolved in PBS over 5 days to induce Cre expression ...
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bioRxiv - Cell Biology 2024Quote: ... Mx1-Cre mice were given 3 intraperitoneal injections over 5 days of 40µg of polyinosinic-polycytidilic acid (poly I:C) (GE Healthcare) dissolved in PBS ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected after 3 days post transfection and purified using GraviTrap TALON columns (GE Healthcare # GE29-0005-94). The columns were first equilibrated with purification buffer (50 mM sodium phosphate pH 7.4 ...
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bioRxiv - Molecular Biology 2023Quote: ... at 55 cm Hg for 1.5 hrs with RNA transfer buffer (3 M NaCl, 0.01 N NaOH) using a VacuGene XL (GE Healthcare). The membrane was rinsed with 2x SSC for 10 min ...
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bioRxiv - Neuroscience 2023Quote: Both structural and functional MRI data were obtained using a 3-T scanner (Discovery 750; GE Healthcare, Milwaukee, WI). High-resolution T1-weighted structural images were captured using a three-dimensional brain-volume sequence (TE = 3.18 ms ...
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Deletion or inhibition of PTPRO mitigates diet-induced hepatic steatosis and inflammation in obesitybioRxiv - Immunology 2022Quote: ... Isoelectric focusing (IEF) was performed using 7-cm Immobiline DryStrips (pH 3–11 nonlinear) (GE Healthcare Life Sciences, USA) in an Ethan IPGphor II Isoelectric Focusing System (GE Healthcare Life Sciences ...
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bioRxiv - Cancer Biology 2022Quote: ... acute- and chronic-type ATL patients with 3% dextran and the density gradient medium Ficoll-Paque Plus (GE Healthcare). For lymphoma-type ATL ...
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bioRxiv - Neuroscience 2022Quote: ... and an aliquot of synaptosomal suspension (2 ml) was placed onto a 3-ml Percoll discontinuous gradient (GE Healthcare) containing (in mM ...
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bioRxiv - Bioengineering 2023Quote: ... with HBS-EP+ as running buffer (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% v/v Surfactant P20, GE Healthcare). Ligands were immobilized on a CM5 chip (GE Healthcare # 29104988 ...
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bioRxiv - Synthetic Biology 2024Quote: ... with HBS-EP+ as the running buffer (10 mM HEPES at pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% v/v Surfactant P20, GE Healthcare). Proteins were immobilized on a CM5 chip (GE Healthcare #29104988 ...
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bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins and His-Atg8 were affinity purified using Glutathione Sepharose 4 Fast Flow (GE Healthcare) or Ni-NTA agarose (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... and incubated with 0.5 ml protein-A-sepharose (nProtein A Sepharose 4 Fast flow, GE healthcare #1705280) at 4 °C for 24 hr ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.5 by gel filtration over Sephadex G25 (GE Healthcare, 4 x HiPrep Desalting 26/10 columns).
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bioRxiv - Biophysics 2021Quote: ... PfK5ΔL6-MD was purified from the clarified lysate at 4°C using an ÄKTA Pure (GE Healthcare) in one day ...
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bioRxiv - Biochemistry 2021Quote: ... 4 °C and injected onto a HiLoad 16/600 Superdex 200 gel filtration column (GE Life Sciences) equilibrated with buffer C (Table S1) ...
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bioRxiv - Cell Biology 2022Quote: ... This was then coupled onto NHS-ester Sepharose 4 Fast Flow resin (GE Healthcare, 17-0906-01) for 4 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... samples were incubated with anti-LptD antibodies and nProtein A Sepharose™ 4 Fast Flow (GE healthcare) at 4°C over-night with slow rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... Supernatant was incubated (2 h, 4 °C) with 4.0 mL of Glutathione Sepharose 4B resin (GE Healthcare) that had been pre-equilibrated in the same buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Sperm were layered onto a discontinuous gradient consisting of 4 mL of 35% Percoll® (GE Healthcare) underlaid with 2 mL of 70% Percoll® ...
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bioRxiv - Immunology 2021Quote: ... Supernatant was harvested after 4-5 days and antibody purified on Protein G Sepharose (Abcam, GE Healthcare) following standard protocols ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were separated by 4-20% SDS-PAGE and transferred to PVDF membranes (GE Healthcare, Piscataway, NJ). Membranes were blocked with 5% BSA in 1x TBS with 0.1% Tween 20 for 1 hour at room temperature with shaking ...
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bioRxiv - Biochemistry 2021Quote: Aβ42 monomers were immobilized by amine coupling onto flowcell 4 on a CM5 sensor chip (GE Healthcare) using a BIAcore 3000 instrument (BIAcore AB) ...
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bioRxiv - Biochemistry 2021Quote: ... the samples were re-adsorbed onto a Glutathione Sepharose 4 Fast Flow 16/10 column (GE Healthcare) and the flow-through was purified by size exclusion chromatography using a Superdex 200 10/300 GL column (GE Healthcare) ...
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bioRxiv - Biophysics 2020Quote: ... the cleared lysate was loaded at 4 °C on an HisTrap HP Ni2+–Sepharose column (GE Healthcare) following the manufacturer’s instructions and further purified by size exclusion chromatography (SEC ...
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bioRxiv - Cell Biology 2021Quote: ... Sperm were layered onto a discontinuous gradient consisting of 4 mL of 35% Percoll® (GE Healthcare) underlaid with 2 mL of 70% Percoll® ...