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Citations for ATTO-TEC GmbH :
151 - 198 of 198 citations for Ovalbumin Atto 594 conjugate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... or 0.1% atto647-DOPE (ATTO-TEC GmbH, Catalog # AD 647–161). The lipid sheets were resuspended in PBS ...
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bioRxiv - Biochemistry 2024Quote: ... with a ∼2-fold molar excess of Atto425-Malemide (ATTO-TEC) for 12 h on ice ...
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bioRxiv - Biophysics 2024Quote: ... was labeled by Atto647N-NHS ester (ATTO-TEC GmbH, AD647N-31) and Atto565-NHS ester (ATTO-TEC GmbH ...
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bioRxiv - Biophysics 2020Quote: ... Proteins that were labeled with Atto488 maleimide (ATTO-TEC GmbH, Siegen, Germany) were titrated into the GUV suspension by 10 μM steps ...
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bioRxiv - Cell Biology 2020Quote: ... Phalloidins were either ATTO488-coupled (#AD488-81, ATTO-TEC, 1:200 dilution), ATTO594-coupled (#AD594-81 ...
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bioRxiv - Cell Biology 2022Quote: ... ATTO390 (AD 390) and ATTO647N (AD 647N) were purchased from ATTO-TEC. Phalloidin conjugated to iFluor 555 (ab176756 ...
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bioRxiv - Biophysics 2023Quote: ... His-Clathrin was labeled with Atto594 NHS-ester (ATTO-TEC, Sigma-Aldrich) according to a previously published protocol (41 ...
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bioRxiv - Cell Biology 2020Quote: ... Tubulin was labeled with fluorescent dyes (NHS-ester atto488 or atto565 (ATTO-TEC)) according to (55) ...
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bioRxiv - Neuroscience 2020Quote: ... CAMK2_1 and Beta Actin_1 were incubated with 200 nmol ATTO565 NHS (ATTO-TEC), dissolved in 50 μL DMF (lumiprobe ...
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bioRxiv - Neuroscience 2020Quote: ... CAMK2_2 and Beta Actin_2 were incubated with 200 nmol ATTO647N NHS (ATTO-TEC), dissolved in 50 μL DMF ...
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bioRxiv - Microbiology 2021Quote: ... pneumoniae ΔmurMN Lipid II was fluorescently labeled with ATTO488 NHS-ester (ATTO-TEC) (NHS-ester:Lipid II = 8:1 mole ratio ...
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bioRxiv - Biochemistry 2023Quote: ... ATTO488-1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (ATTO488) was obtained from ATTO-TEC GmbH (Siegen ...
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bioRxiv - Systems Biology 2023Quote: ... pH 8.3) and NHS-functionalized thiorhodamine (Thio12, cat: AD-Thio12-41, ATTO-TEC GmbH) was added to an approximate final molecular weight ratio of 1:25 HDL to SOG ...
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bioRxiv - Molecular Biology 2024Quote: ... Unlabelled TDP-43 CTD monomers were labelled with Atto647N-NHS dyes (ATTO-TEC GmbH) with stochiometric amount ...
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bioRxiv - Biophysics 2020Quote: ... was incubated with a 10 x excess of Tetrazine-ATTO655 (ATTO-TEC, AD 655-2505) at RT and purified again after 15 min.
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bioRxiv - Biochemistry 2024Quote: The labeling of CXCR4 nanobodies with ATTO565 fluorescent dyes (ATTO-TEC, #AD565-41, #AD565-31) using thiol-maleimide coupling and N-hydroxy-succinimide (NHS ...
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bioRxiv - Cell Biology 2021Quote: ... 10% PS in 84.5% POPC doped with either 0.1% NBD-DPPE or Atto647N-DOPE (ATTO-TEC) for 30 min and washed three times ...
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bioRxiv - Neuroscience 2022Quote: ... were covalently labeled with ATTO647N succinimidyl ester or ATTO594 succinimidyl ester dye (ATTO-TEC, Siegen, Germany) using manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: Atto488-DPPE (1,2-dipalmitoyl-SN-glycero-3-phosphoethanolamine) and Atto550-DPPE were purchased from ATTO-TEC, all other lipids were from Avanti Polar Lipids ...
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bioRxiv - Biophysics 2023Quote: ... The Atto655-dyes labeled 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE-Atto655) were purchased from ATTO-TEC GmbH and dissolved in chloroform at a concentration of 0.01 mg/mL and 1 mg/mL for stock solution.
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bioRxiv - Cell Biology 2020Quote: ... Frog tubulin was purified from Xenopus laevis egg extract and directly labeled with NHS-ester atto565 (ATTO-TEC) according to (56).
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bioRxiv - Biochemistry 2020Quote: For smFRET experiments engineered cysteines of NBD1 constructs were labeled with ATTO488 and Alexa647 maleimide dyes (ATTO-TEC GmbH AD 488-41 and ThermoFisher A20347 ...
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bioRxiv - Cell Biology 2022Quote: ... Confocal volume estimation was carried out by ten 1-min FCS measurements of 10 nM Atto488 (ATTO-TEC) in water ...
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bioRxiv - Biophysics 2022Quote: ... were purchased from Avanti Polar Lipids (Alabaster, AL) while 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-ATTO488 (DOPE-ATTO488, AD488-161) was purchased from ATTO-TEC. Lipid stock concentrations of 5 mg/mL were prepared by addition of chloroform from initial stocks of 10 mg/mL except for DOPE-ATTO488 where the lipid were solubilized in chloroform to a final concentration of 1 mg/mL ...
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bioRxiv - Biophysics 2020Quote: ... the actin skeleton of the CMs was stained with a fluorescent dye (Atto633-phalloidin, ATTO-TEC GmbH, Siegen, Germany). Plunge freezing of CMs was performed with a grid plunger (Leica EM GP) ...
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bioRxiv - Cell Biology 2022Quote: ... gels were washed in PBS and stained with 30 µg/mL NHS-ester conjugated to ATTO488 (ATTO-TEC GmbH) for 2 h at RT ...
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bioRxiv - Biophysics 2020Quote: ... Afterwards the solution was diluted 100-fold using 1x polymerization buffer containing 70 nM Atto590-phalloidin (ATTO-TEC, Siegen, Germany). A microscope coverslip was spotted with 10 μL of the solution containing the actin filaments ...
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bioRxiv - Cell Biology 2021Quote: ... were produced by mixing 95 mol % 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) with 5 mol % of the respective phosphatidylinositol together with 0.1% Atto647N-DOPE (ATTO-TEC) or Rhodamine-DOPE ...
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bioRxiv - Biophysics 2020Quote: GUVs were prepared as previously described with 0.5 % of the fluorescently labeled lipid Atto647N PtdEnt (ATTO-TEC GmbH, Siegen, Germany). 50 μl of the GUV suspension was carefully transferred into the microscopy chamber Nunc® Lab-Tek® II chambered coverglass (Thermo Fisher Scientific Inc. ...
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bioRxiv - Plant Biology 2023Quote: ... pH 8.3 and stained in 20 μg/mL N-Hydroxysuccinimide (NHS) ester-ATTO488 (ATTO-TEC GmbH, Art. Nr.: AD 488) in 0.1 M NaHCO3 ...
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bioRxiv - Cell Biology 2020Quote: Fibrinogen-biotin-ATTO490LS and Fibrinogen-biotin-Alexa647 were generated by mixing Fibrinogen (4mg/ml in Fibrinogen buffer) with a 3-fold molar excess of ATTO490LS NHS ester (ATTO-TEC) or Alexa647 NHS ester (ThermoFisher ...
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bioRxiv - Systems Biology 2020Quote: ... Singlet oxygen generators in form of NHS-functionalized thiorhodamine (cat: AD-Thio12-41) and azide-functionalized methylene blue (cat: AD-MB2-31) were purchased from ATTO-TEC GmbH (Siegen ...
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bioRxiv - Biophysics 2022Quote: ... DGS-NTA-Ni (790404, Avanti), DSPE-RGD (870295, Avanti), DSPE-PEG-cRGDyk (LP096262-2K, Biopharma PEG) and DOPE-ATTO390 (390-161, ATTO-TEC).
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bioRxiv - Cell Biology 2023Quote: ... Confocal volume estimation was carried out by ten FCS-measurements of 1 min of 10 nM Atto488 (AD 488-21, ATTO-TEC) in double-distilled water ...
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bioRxiv - Biochemistry 2024Quote: ... the extinction coefficient of the fluorophores was used to determine the concentration (120000 M-1cm-1 for ATTO590 and 90000 M-1cm-1 for ATTO488, source: ATTO-TEC). To determine the binding affinity of each probe to the RNA of interest ...
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bioRxiv - Molecular Biology 2021Quote: ... After another wash with 3% BSA samples were incubated in home-made click-labelling buffer (50 mM Tris, 4 mM CuSO4, 10 mM Ascorbic acid and 60 μM Atto568 azide (ATTO-TEC GmbH)) for 20 minutes in the dark ...
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bioRxiv - Neuroscience 2021Quote: ... the following tetrazine derivatives of fluorescent dyes were used: ATTO655-methyltetrazine (ATTO655-me-tz; ATTO-TEC GmbH, cat. no. AD 655-2502), ATTO488-tetrazine (ATTO488-tz ...
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bioRxiv - Biophysics 2022Quote: ... purified protein was dissolved in 2 M guanidine hydrochloride and the protein was allowed to react with Atto488 maleimide (ATTO-TEC, Germany) at 1:1 molar ratio at pH 6.8 ...
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bioRxiv - Biophysics 2024Quote: ... buffer was labelled in a one-pot approach with a 3-fold molar excess of an equimolar mix of maleimide-Atto550 and maleimide-Atto647N FRET pair (ATTO-TEC, Germany) along with 3-fold molar excess of single-stranded methyltetrazine-modified DNA oligonucleotide (Biomers ...
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bioRxiv - Neuroscience 2020Quote: ... Labeling was performed with 1:500 biotinylated WFA or HABP followed by 1:500 streptavidin-Atto647N (#AD 647-61; ATTO-TEC GmbH, Germany), or 1:500 anti-Neurocan and 1:500 nanobody anti-mouse IgG conjugated to STAR635P (#N1202-Ab635P ...
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bioRxiv - Cell Biology 2024Quote: 8 µl of 0.1 mg/ml biotinylated protein CD95L-IZ-biotin was mixed with 3.1 µl of ATTO594 NHS-ester (stock concentration of 18 µM, ATTO-TEC GmbH, Siegen, Germany) and 11.1 µl of the reaction mix was incubated at 4 °C overnight ...
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bioRxiv - Biophysics 2020Quote: The ENTH WT (C96A A155C) domain and the ENTH R114A (C96A A155C) were labeled with Atto488 maleimide (or Atto532 maleimide) (ATTO-TEC GmbH, Siegen, Germany) by cysteine modification ...
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bioRxiv - Biophysics 2020Quote: ... The fluorophores were excited at the optimal wavelength and detected at the optimal emission wavelength according to the manufacturer (ATTO-TEC GmbH, Siegen, Germany). All fluorophores were excited at low laser powers (5% to 8% ...
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bioRxiv - Cell Biology 2021Quote: ... 2.5% biotinyl cap PE (Avanti, Catalog # 870273), 0.5% PEG5000-PE (Avanti, Catalog # 880230, and 0.2% atto390-DOPE (ATTO-TEC GmbH, Catalog # AD 390–161) for labeled lipid bilayers ...
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bioRxiv - Genomics 2023Quote: ... 2 μl of 0.5 mM 5’ amine-modified DNA probes (Integrated DNA technologies) is mixed with 1 μl of 10 mM ATTO488-NHS ester (ATTO-TEC AD 488-31), ATTO 643-NHS ester (ATTO-TEC AD 643-31 ...
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bioRxiv - Cell Biology 2023Quote: ... 1% biotinyl cap PE (Avanti, Catalog # 870273), 0.1% PEG5000-PE (Avanti, Catalog # 880230, and 0.1% atto390-DOPE (ATTO-TEC GmbH, Catalog # AD 390–161) or 0.1% atto647-DOPE (ATTO-TEC GmbH ...
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bioRxiv - Cell Biology 2023Quote: ... Catalog # 850457), 2% DGS-NTA (Avanti, Catalog # 790404), 0.1% PEG5000-PE (Avanti, Catalog # 880230, and 0.1% atto390-DOPE (ATTO-TEC GmbH, Catalog # AD 390– 161). The lipid sheets were resuspended in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Catalog # 850457), 10% DOPS (Avanti, Catalog # 840035), 0.1% PEG5000-PE (Avanti, Catalog # 880230, and 0.1% atto390-DOPE (ATTO-TEC GmbH, Catalog # AD 390–161). The lipid sheets were resuspended in PBS ...