Labshake search
Citations for Dianova :
151 - 200 of 207 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: ... incubated for 1 hour in the dark at room temperature with the secondary antibody (Dianova, 715-096-150). After removal of the 8 well silicone grid ...
-
bioRxiv - Physiology 2023Quote: ... sections were incubated with the secondary antibody (anti-goat-AlexaFluor488, 705-546-147, Dianova, Hamburg, Germany, 1:200) for 24 hours (4°C) ...
-
bioRxiv - Immunology 2020Quote: ... was expressed in HEK293T cells and immobilized onto 96-well plates from cell culture supernatant via anti-rabbit IgG (Dianova, 111-035-045) antibodies ...
-
bioRxiv - Cancer Biology 2022Quote: ... Mutant IDH1 expression was analyzed with western blot and immunocytochemical staining using IDH1R132H specific antibody (DIA-H09, Dianova, Germany). 2-HG production was assessed via D-2-Hydroxyglutarate (D2HG ...
-
bioRxiv - Developmental Biology 2020Quote: ... Following secondary antibodies were used: polyclonal donkey anti-rabbit conjugated to Alexa 488 (1:500; 711-547-003, Dianova), polyclonal donkey anti-rabbit conjugated to Alexa 555 (1:500 ...
-
bioRxiv - Cell Biology 2019Quote: ... Goat anti-mouse IgG HRP conjugated (ab97023) and goat anti-rabbit IgG HRP conjugated antibody (Dianova, 111-035-144) were used as secondary antibodies ...
-
bioRxiv - Cell Biology 2019Quote: ... Secondary anti-rabbit and anti-mouse antibodies coupled to Cy3 or Cy5 fluorophores were purchased from Dianova (Hamburg, Germany).
-
bioRxiv - Neuroscience 2020Quote: ... Secondary goat antibodies were used with 1:200 dilution: Alexa Fluor 488 conjugated anti-chicken (Dianova, 703-45-155), Alexa Fluor 633 conjugated anti-mouse (Invitrogen ...
-
bioRxiv - Genetics 2022Quote: ... the membrane was incubated with corresponding secondary antibodies (peroxidase conjugated Goat Anti-Rabbit IgG (Cat.# 111-035-046, Dianova) or peroxidase conjugated Goat Anti-Mouse IgG (Cat.# 115-035-062 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Anti-mouse and anti-rabbit HRP-conjugated or Alexa Fluor488-conjugated secondary antibodies were purchased from Dianova (Hamburg, Germany) and Invitrogen (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2022Quote: ... DG75 cells with 5 μg/ml anti-human IgM (DIANOVA) for 3 min ...
-
bioRxiv - Genomics 2020Quote: ... We used rabbit anti-RpCENH3 (diluted 1:200) as primary antibody and detected it with Cy3-conjugated anti-rabbit IgG (Dianova) secondary antibody (diluted 1:200) ...
-
bioRxiv - Neuroscience 2020Quote: ... non-specific binding was blocked for 40 min by treating sections with 5% normal serum of the secondary antibody host animal (goat serum: S-1000, Vector; rabbit serum: S-5000, Vector; donkey serum: 017-000-001, Dianova) diluted in TBS and supplemented with 0.3% Triton X-100 to permeabilize cell membranes ...
-
bioRxiv - Cancer Biology 2020Quote: ... Secondary antibody was incubated for 1 h at 25 °C with donkey-anti-rabbit IgG POD (1:30000, Dianova, Hamburg), donkey-anti-goat IgG POD (1:120000 ...
-
bioRxiv - Neuroscience 2019Quote: ... the membrane was washed thoroughly in blocking buffer and incubated with the fluorescently labeled secondary donkey polyclonal anti-mouse antibody (1:1000; Cat. No. 715-175-150, Dianova) for 1 h at RT ...
-
bioRxiv - Neuroscience 2020Quote: ... and afterwards with a Cy3-coupled anti-mouse secondary antibody (goat, 1:500, 2h, cat#115-165-166, RRID: AB_2491007, Dianova, Germany). Finally ...
-
bioRxiv - Cell Biology 2022Quote: ... Primary antibodies in immunoblots were detected with secondary alkaline phosphatase-coupled anti-mouse or anti-rabbit IgG (1:5000, Dianova) and the blots visualized with 5-Bromo-4-chloro-3-indolyl phosphate (BCIP ...
-
bioRxiv - Neuroscience 2019Quote: ... Afterwards the preparations were washed with PBS 8 x 30 min and then incubated with secondary antibodies (donkey anti-chicken Cy2, 1:400, Dianova, cat# 703-505-155 ...
-
bioRxiv - Immunology 2021Quote: ... Proteins were then inactivated and sections incubated with αCD4 (4SM95, eBioscience) followed by incubation with rabbit anti-rat secondary antibody (Dianova), then with the EnVision+ System-HRP Labelled Polymer Anti-Rabbit and finally with OPAL-670 diluted in amplifier diluent (Akoya Biosciences) ...
-
bioRxiv - Microbiology 2021Quote: ... Human anti-spike RBD monoclonal antibodies were applied at 1 μg/ml and detected using horseradish peroxidase (HRP)-conjugated anti-human IgG (Dianova) and the HRP substrate 1-step Ultra TMB (Thermo Fisher Scientific) ...
-
bioRxiv - Bioengineering 2019Quote: ... The membrane was washed with TBST (3x 10 min) and incubated with the secondary goat anti-rabbit whole IgG horseradish peroxidase (HRP) conjugated antibody (Dianova) diluted 1:5000 in TBST containing 2.5% nonfat dried milk ...
-
bioRxiv - Molecular Biology 2020Quote: ... Kidney sections were antigen retrieved with citrate buffer and immunostained with an anti-CD31 antibody (1:20, Dianova, DIA-310). Images were taken in a Nikon A1R confocal microscope.
-
bioRxiv - Microbiology 2020Quote: ... and finally incubated with a 1:20,000 dilution of a peroxidase-conjugated secondary goat anti-rabbit antibody (Dianova, Hamburg, Germany). Signals were visualised using the ECL kit (Clarity Western ECL ...
-
bioRxiv - Molecular Biology 2019Quote: ... for 1 h, and after extensive washing they were incubated with secondary antibody (goat anti-rat-IgG, HRP-coupled (Dianova), 1:500 ...
-
bioRxiv - Microbiology 2020Quote: ... followed by three washes in TBS-T and incubation with donkey anti-mouse horseradish peroxidase (HRP)-coupled secondary antibody (Dianova). After three washes ...
-
bioRxiv - Neuroscience 2021Quote: ... Primary hippocampal cultures were further processed for immunochemistry using antibodies (anti-GABA 1:750, Sigma, with anti-rabbit IgG AMCA 1:200, Dianova, and anti-MAP2 1:500 ...
-
bioRxiv - Cell Biology 2020Quote: ... Primary antibodies in immunoblots were visualized using phosphatase-coupled anti-mouse (1:1,000 dilution; #115-055-62, Dianova (Hamburg, Germany)) or anti-rabbit antibodies (1:1,000 dilution ...
-
bioRxiv - Bioengineering 2022Quote: ... before the incubation with the last antibody carrying AS635P (#ST635P-1055, Abberior), AS580 (#ST580-1002, Abberior) or AF488 (#705-545-147, Dianova). This antibody was applied for 30 minutes at room temperature ...
-
bioRxiv - Bioengineering 2022Quote: ... samples were incubated with fluorophore-conjugated secondary antibodies (AS635P #ST635P-1001; AS580 #ST580-1001, both Abberior; Cy3 #715-165-150, Dianova) or nanobodies (AS635P ...
-
bioRxiv - Cell Biology 2023Quote: ... Blots were washed thrice with TBS-T and incubated with one of the following secondary antibodies for 1 h at RT: HRP-goat anti-rabbit (1: 5,000, goat pAb, 111-035-003, Dianova, Germany) or HRP-goat anti-mouse (1:5,000 ...
-
bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated with F(ab′)2 anti-rabbit IgG (H+L)-HRPO secondary antibody (goat, 1:10000, 111-036-045, Dianova). Chemiluminescence was induced by using Clarity Western ECL Substrate (Bio-Rad).
-
bioRxiv - Neuroscience 2024Quote: The following secondary antibodies were used for immunoblots: horseradish peroxidase-conjugated donkey anti-goat IgG (H+L) (Dianova, 1:5,000), donkey anti-sheep IgG goat (Jackson Immuno Research ...
-
bioRxiv - Neuroscience 2023Quote: ... slides were washed 3x10 minutes in PBS and then incubated for two hours at room temperature with the appropriate secondary antibody (Dianova). Sections were then washed 2 x 5 mins and stained with Hoechst/DAPI (1:5000 in PBS ...
-
bioRxiv - Cell Biology 2022Quote: The following HRP-coupled secondary antibodies have been used: peroxidase-coupled-goat-anti-rabbit or –goat-anti-mouse (Dianova, Hamburg, Germany) was detected using the ECL-system.
-
bioRxiv - Neuroscience 2020Quote: ... Sections were washed 3 times in PBS and incubated for 2 h with adequate secondary antibody (Dianova, Hamburg, Germany; Table 1) solution without Triton™-X-100 ...
-
bioRxiv - Microbiology 2019Quote: ... The slides were washed thrice with PBS/Tween 20 followed by incubation with the secondary antibody Cy3-conjugated F(ab)2 goat-anti-rabbit IgG (Dianova, Hamburg) (diluted 1:500 in PBS/Tween 20 ...
-
bioRxiv - Microbiology 2021Quote: ... Viral NS5 proteins were visualised with an NS5 specific antiserum (Biozol, Germany) and a Cy3 coupled donkey anti-rabbit IgG antibody (Dianova, Germany). HepG2 cells were transfected with the pcDNA3.4-synNS5-FLAG ...
-
Development of immortalized rhesus macaque kidney cells supporting infection with a panel of virusesbioRxiv - Microbiology 2022Quote: ... cells were incubated with 50 μl/well hybridoma 4G2 supernatant followed by 50μl secondary anti-mouse horseradish peroxidase (HRP)-conjugated antibody (1:1000, Dianova, Hamburg, Germany). Antibody incubation steps were followed by washing steps ...
-
bioRxiv - Plant Biology 2024Quote: ... 37°C) and secondary rabbit Cy3-Antibodies (BSA/PBS for 3.5h at 37°C; Jackson ImmunoResearch/USA supplied by Dianova/Hamburg). After repeated washes with PBS and H20 the embryos were embedded in Citifluor antifadent mounting medium and covered with a coverslip ...
-
bioRxiv - Neuroscience 2023Quote: ... the preparations were incubated with secondary antibodies for 2 hours at RT: goat-α-HRP-AlexaFluor488 (1:200, JIR/Dianova, AB_2338965), goat-anti-α-mouse-StarRed (1:200 ...
-
bioRxiv - Developmental Biology 2020Quote: ... The following secondary antibodies were used at a concentration of 1:500: Cy2: anti-chicken (donkey polyclonal, Dianova, 703-225-155, RRID:AB_2340370); Alexa Fluor 488 ...
-
bioRxiv - Neuroscience 2020Quote: ... incubated in Alexa 488 conjugated goat anti-mouse secondary antibody for 5 days at 4°C (1:200 in blocking solution, 115-545-062, Dianova, Hamburg, Germany) and rinsed in PBS-1% Triton X-100 (2×2h ...
-
bioRxiv - Physiology 2022Quote: The following horseradish peroxidase-labeled IgG secondary antibodies were used for Western blotting: gam pox (WB 1:3000, Dianova, 115-035-003), garb pox (WB 1:3000 ...
-
bioRxiv - Cell Biology 2024Quote: ... the membranes were incubated at room temperature for 1 h with HRP-conjugated anti-rabbit or anti-mouse secondary antibodies (Dianova, Hamburg, Germany) diluted 1:5000 in blocking buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... the same amount of chromatin was incubated with rat anti-HMR 2C10 antibody pre-coupled to Protein A/G Sepharose through a rabbit IgG anti-rat (Dianova, 312-005-046). Samples were sequenced (single-end ...
-
bioRxiv - Biochemistry 2021Quote: ... while rat anti-HMR and anti-LHR were incubated with beads that were pre-coupled with 12 μL of a rabbit anti-rat bridging antibody (Dianova, 312-005-046). FLAG-IPs in non-FLAG containing nuclear extracts were used as mock controls for FLAG-IPs ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... For detection of the primary antibodies the membrane was incubated for one hour with goat anti-mouse (α5, 12G10 anti-α-tubulin), anti-rabbit (β2), and anti-chicken (β1, β3) secondary antibodies conjugated with horseradish peroxidase (Dianova, Hamburg, Germany) and developed with 4-chloro-1-naphtol as previously described (27).
-
bioRxiv - Cell Biology 2022Quote: ... Cells were washed three times with 1x PBS followed by incubation with the secondary antibody on the shaker for an hour: goat IgG anti-mouse IgG Cy3 (Dianova, #115-165-146), goat IgG anti-rabbit IgG Cy2 (Dianova ...
-
bioRxiv - Developmental Biology 2023Quote: Sections were washed in PBS and incubated with a combination of Alexa fluor-conjugated secondary antibody (1:750, Invitrogen 11058, A11076, A21202, A21206, A21207, A21208, A31571, A31573; 1:750, Dianova 127-495-099) and counterstained with 250ng/ml of Hoechst 33342 ...
-
bioRxiv - Immunology 2020Quote: ... rat anti-CD31 for endothelial cells (1:200 dilution; Dianova GmbH, Hamburg, Germany). Sections were incubated with Alexa Fluor 488- and 594-conjugated secondary antibodies (Invitrogen ...