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Citations for Dianova :
251 - 294 of 294 citations for Indusatumab Anti GUCY2C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... Following secondary antibodies were used: polyclonal donkey anti-rabbit conjugated to Alexa 488 (1:500; 711-547-003, Dianova), polyclonal donkey anti-rabbit conjugated to Alexa 555 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary goat antibodies were used with 1:200 dilution: Alexa Fluor 488 conjugated anti-chicken (Dianova, 703-45-155), Alexa Fluor 633 conjugated anti-mouse (Invitrogen ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The primary antibody was detected using a goat-anti-mouse secondary antibody conjugated with horseradish peroxidase (Dianova, Hamburg, Germany). The staining of the precipitated polypeptide-antibody complexes was performed by addition of 60 mg 4-chloro-1 naphtol (Sigma-Aldrich ...
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bioRxiv - Genetics 2022Quote: ... the membrane was incubated with corresponding secondary antibodies (peroxidase conjugated Goat Anti-Rabbit IgG (Cat.# 111-035-046, Dianova) or peroxidase conjugated Goat Anti-Mouse IgG (Cat.# 115-035-062 ...
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bioRxiv - Neuroscience 2021Quote: ... secondary antibodies were applied for 1 hour (all antibodies were purchased from Dianova: Cy2 goat anti mouse (115-225-071), Cy2 goat anti rat (112-225-143) ...
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bioRxiv - Cancer Biology 2020Quote: ... Secondary antibody was incubated for 1 h at 25 °C with donkey-anti-rabbit IgG POD (1:30000, Dianova, Hamburg), donkey-anti-goat IgG POD (1:120000 ...
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bioRxiv - Neuroscience 2020Quote: ... and afterwards with a Cy3-coupled anti-mouse secondary antibody (goat, 1:500, 2h, cat#115-165-166, RRID: AB_2491007, Dianova, Germany). Finally ...
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bioRxiv - Immunology 2021Quote: ... Proteins were then inactivated and sections incubated with αCD4 (4SM95, eBioscience) followed by incubation with rabbit anti-rat secondary antibody (Dianova), then with the EnVision+ System-HRP Labelled Polymer Anti-Rabbit and finally with OPAL-670 diluted in amplifier diluent (Akoya Biosciences) ...
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bioRxiv - Microbiology 2021Quote: ... Human anti-spike RBD monoclonal antibodies were applied at 1 μg/ml and detected using horseradish peroxidase (HRP)-conjugated anti-human IgG (Dianova) and the HRP substrate 1-step Ultra TMB (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... permealized and stained with rat mAb to ADAP (clone 92B2G10) in combination with anti-rat IgG-conjugated with FITC (Dianova). F-actin was stained with FITC- or TRITC-phalloidin (both from Sigma Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... Goat Fab anti-Rabbit IgG Alexa Fluor 488 (111-547-008) and Goat IgG anti-Rabbit IgG Cy3 (111-165-003) were purchased from DIANOVA GmbH Hamburg ...
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bioRxiv - Molecular Biology 2020Quote: ... Kidney sections were antigen retrieved with citrate buffer and immunostained with an anti-CD31 antibody (1:20, Dianova, DIA-310). Images were taken in a Nikon A1R confocal microscope.
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bioRxiv - Microbiology 2020Quote: ... and finally incubated with a 1:20,000 dilution of a peroxidase-conjugated secondary goat anti-rabbit antibody (Dianova, Hamburg, Germany). Signals were visualised using the ECL kit (Clarity Western ECL ...
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bioRxiv - Microbiology 2020Quote: ... followed by three washes in TBS-T and incubation with donkey anti-mouse horseradish peroxidase (HRP)-coupled secondary antibody (Dianova). After three washes ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies in immunoblots were visualized using phosphatase-coupled anti-mouse (1:1,000 dilution; #115-055-62, Dianova (Hamburg, Germany)) or anti-rabbit antibodies (1:1,000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... followed by sequential 1-h incubation at room temperature with in-house made rabbit polyclonal antibodies against HK and peroxidase-labelled donkey anti-rabbit antibodies (Dianova). Both antibodies were diluted in PBS buffer containing 10% normal horse serum (Dianova) ...
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bioRxiv - Neuroscience 2024Quote: The following secondary antibodies were used for immunoblots: horseradish peroxidase-conjugated donkey anti-goat IgG (H+L) (Dianova, 1:5,000), donkey anti-sheep IgG goat (Jackson Immuno Research ...
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bioRxiv - Pathology 2023Quote: ... Muscularization of distal intrapulmonary vessels were determined on double immunostained tissue sections with anti- CD31 (rat monoclonal, #DIA-310, Dianova) and anti-α-SMA (rabbit monoclonal ...
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bioRxiv - Cell Biology 2023Quote: ... Blots were washed thrice with TBS-T and incubated with one of the following secondary antibodies for 1 h at RT: HRP-goat anti-rabbit (1: 5,000, goat pAb, 111-035-003, Dianova, Germany) or HRP-goat anti-mouse (1:5,000 ...
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bioRxiv - Cell Biology 2023Quote: ... and the respective secondary antibodies in blocking solution for 90 min at RT in the dark: anti-rabbit-Cy3 (1:600, goat, 111-165-144, Dianova), anti-mouse-Alexa488 (1:600 ...
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bioRxiv - Developmental Biology 2023Quote: ... membranes were incubated with F(ab′)2 anti-rabbit IgG (H+L)-HRPO secondary antibody (goat, 1:10000, 111-036-045, Dianova). Chemiluminescence was induced by using Clarity Western ECL Substrate (Bio-Rad).
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bioRxiv - Developmental Biology 2023Quote: ... and after standard treatments the Salm protein was eluted from Histidine columns and visualized by western blot using mouse anti-His (1:1000; Dianova) and anti-Salm (1:200 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and then incubated for 1 day at 4°C with Alexa 647 anti-rabbit (Dianova, 711-606-152, 1:300), Cy3 anti-mouse (Dianova 715-166-151 ...
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bioRxiv - Immunology 2024Quote: ... followed by incubation in colloidal gold conjugated secondary antibody diluted 1:100 in blocking buffer for 24 h at 4°C (goat anti-mouse colloidal gold-conjugated secondary antibody 12 nm, Dianova GmbH ...
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bioRxiv - Neuroscience 2020Quote: ... The neurons were washed and incubated with 1:200 Cy3-conjugated goat anti-mouse IgG or (#115-165-146; Dianova, Germany) or Cy3-conjugated streptavidin (#016-160-084 ...
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bioRxiv - Biochemistry 2022Quote: ... Both primary antibodies were detected with an HRP-conjugated anti-rabbit secondary antibody (Dianova #111-035-045 at 1:10000 dilution) for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 infection was visualized by use of α-S mAb (kindly provided by Peter Miethe, fzmb, Bad Langensalza, Germany) and Cy2-conjugated goat anti-mouse IgG (Dianova). Nuclei were counterstained with 4’6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Pathology 2020Quote: ... and antigen retrieval with 1:20 proteinase K/TE buffer and rat anti-mouse F4/80 (1:100) (T-2006, Dianova, Germany) incubated at 4°C overnight ...
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bioRxiv - Microbiology 2021Quote: ... Viral NS5 proteins were visualised with an NS5 specific antiserum (Biozol, Germany) and a Cy3 coupled donkey anti-rabbit IgG antibody (Dianova, Germany). HepG2 cells were transfected with the pcDNA3.4-synNS5-FLAG ...
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Development of immortalized rhesus macaque kidney cells supporting infection with a panel of virusesbioRxiv - Microbiology 2022Quote: ... cells were incubated with 50 μl/well hybridoma 4G2 supernatant followed by 50μl secondary anti-mouse horseradish peroxidase (HRP)-conjugated antibody (1:1000, Dianova, Hamburg, Germany). Antibody incubation steps were followed by washing steps ...
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bioRxiv - Developmental Biology 2020Quote: ... The following secondary antibodies were used at a concentration of 1:500: Cy2: anti-chicken (donkey polyclonal, Dianova, 703-225-155, RRID:AB_2340370); Alexa Fluor 488 ...
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bioRxiv - Neuroscience 2020Quote: ... incubated in Alexa 488 conjugated goat anti-mouse secondary antibody for 5 days at 4°C (1:200 in blocking solution, 115-545-062, Dianova, Hamburg, Germany) and rinsed in PBS-1% Triton X-100 (2×2h ...
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bioRxiv - Immunology 2020Quote: ... For the detection of antigen-bound antibodies the wells were incubated with 50 μl of horseradish peroxidase (HRP)-labeled anti-mouse IgM or IgG antibodies (Dianova, Hamburg, Germany) for 45 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... 150 mM NaCl pH 7.4 with added 0.1% Tween-20) and afterwards incubated with the appropriate secondary antibodies (horseradish-peroxidase-conjugated goat anti-rabbit antibody 1:2,000 (Dianova; Goat F(ab’)2 anti-Rabbit IgG (H+L)-HRPO ...
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bioRxiv - Immunology 2024Quote: ... The efficiency of transduction was assessed on the fourth day following transduction via flow cytometry using an anti-human goat F(ab) IgG (H+L) PE antibody (Dianova, Hamburg, Germany). The quantity of effector cells employed for functional assays was adjusted based on the determined transduction efficiency ...
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bioRxiv - Cancer Biology 2024Quote: ... Goat anti-mouse IgG HRP-linked antibody (#115-035-062) and goat anti-rabbit IgG HRP-linked antibody (#111-035-144) were purchased from Dianova (Hamburg, Germany). The following antibodies were used in immunofluorescence staining ...
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bioRxiv - Cell Biology 2024Quote: ... Labelling was visualized with a mixture of the above-mentioned secondary antibodies and donkey-anti-rabbit IgG coupled to Cy3 (dilution 1:250; Dianova, Hamburg, Germany). The two cone opsin antisera sc-14363 and JH492 have been shown to robustly label the SWS1 opsin and LWS opsin ...
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bioRxiv - Immunology 2021Quote: ... and the constant region of rabbit IgG1 heavy chain (Fc) were immobilized onto 96-well plates via anti-rabbit IgG (Dianova, 711-005-152). Human mAbs were applied and detected using HRP-conjugated anti-human IgG (Dianova ...
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bioRxiv - Genetics 2020Quote: ... After washing sections were incubated in a secondary Biotin-conjugated antibody (goat anti mouse Biotin-SP, 1:1000, #115-035-003, Dianova GmbH, Hamburg, Germany) in blocking solution for 1h at RT and 2 days at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... while rat anti-HMR and anti-LHR were incubated with beads that were pre-coupled with 12 μL of a rabbit anti-rat bridging antibody (Dianova, 312-005-046). FLAG-IPs in non-FLAG containing nuclear extracts were used as mock controls for FLAG-IPs ...
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bioRxiv - Immunology 2020Quote: ... was expressed in HEK293T cells and immobilized onto 96-well plates from cell culture supernatant via anti-rabbit IgG (Dianova, 111-035-045) antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed three times with 1x PBS followed by incubation with the secondary antibody on the shaker for an hour: goat IgG anti-mouse IgG Cy3 (Dianova, #115-165-146), goat IgG anti-rabbit IgG Cy2 (Dianova ...
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bioRxiv - Immunology 2020Quote: ... Complexes of NCR-Fc fusion proteins (1 to 2 μg per staining) and PE-labeled goat anti-hIgG Fc (Dianova; 1:100 in FACS buffer) were allowed to form for 30 min before being added to cells for 60 min on ice ...
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bioRxiv - Microbiology 2021Quote: ... the blots were washed 3x with PBS-T before they were incubated for 1 h at room temperature with peroxidase-coupled goat anti-mouse antibody (Dianova, 115-035-003, 1:10,000). Finally ...