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Citations for Sciex :
101 - 150 of 156 citations for Microtubule Associated Protein 1 Light Chain 3 Gamma MAP1LC3C Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... the processed mrkw files containing protein information from PeakView were loaded into MarkerView (Version 1.2.1.1, AB Sciex) for normalization of protein intensity (total area sums ...
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bioRxiv - Cell Biology 2021Quote: Mass spectrometric data was searched using the database search engine Protein Pilot [23] (SCIEX 5.0, revision 4769) with the Paragon algorithm (5.0.0.0.4767) ...
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bioRxiv - Immunology 2019Quote: ... raw files obtained by DDA analysis of eight secreted protein samples were searched using ProteinPilot software (SCIEX), and search results were then imported into Spectronaut software (Biognosys ...
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bioRxiv - Physiology 2024Quote: ... The proteins were identified by MALDI TOF/TOF mass spectrometry (AB SCIEX TOF/TOF™ 5800 System) using Swiss-Prot database.
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bioRxiv - Microbiology 2023Quote: The digested proteins from each gel spot were analyzed using the NanoLC Ultra® 2Dplus System (SCIEX) combined with the cHiPLC® system in Trap-Elute mode ...
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bioRxiv - Systems Biology 2020Quote: ... and injected into an online LC-MS/MS workflow (500 nL/min) using a 3 h gradient run using a Sciex TripleTOF 5600 instrument (Sciex; Framingham, MA). Raw spectra files were converted into Mascot Generic File format (MGF ...
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bioRxiv - Physiology 2020Quote: ... of each sample were loaded onto a ChromXP C18 peptide trap (15 cm x 200 μm, 3 μm particle size, 120 Å, SCIEX, USA). The peptides were separated with a 60 min gradient of 5 – 40% acetonitrile ...
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bioRxiv - Plant Biology 2021Quote: ... approximately 4 µg) were eluted from a cHiPLC C18 column (15 cm × 200 μm, 3 μm, ChromXP C18CL, 120 Å, 25°C, Sciex, USA) using a linear gradient of 2-35% of solvent B (90% acetonitrile with 0.1% formic acid ...
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bioRxiv - Microbiology 2022Quote: ... It was coupled with a chromXP reversed-phase 3-µm C18-CL trap column (350 μm by 0.5 mm, 120 Å; Eksigent; AB Sciex, MA, USA) and a nanoViper C18 separation column (75 μm by 250 mm ...
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bioRxiv - Physiology 2023Quote: Peptides obtained from each of the five NP mixtures were separately reconstituted according in a solution of 0.1% formic acid and 3% acetonitrile (34) spiked with 5 fmol μL PepCalMix from SCIEX (Framingham, MA, USA). Reconstitution volumes varied by NP types to allow for constant peptide quantity for MS injection between samples regardless of starting volume (240 ng ...
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bioRxiv - Physiology 2023Quote: Peptides obtained from each of the five NP mixtures were separately reconstituted in a solution of 0.1% formic acid and 3% acetonitrile [37] spiked with 5 fmol μL PepCalMix from SCIEX (Framingham, MA, USA). Reconstitution volumes varied NPs to allow for constant peptide quantity for MS injection between samples regardless of starting volume (240 ng ...
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bioRxiv - Biochemistry 2021Quote: ... The obtained protein peak areas were exported to Markerview™ version 1.3 (AB Sciex, Foster City, CA, USA) for further data refinement ...
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bioRxiv - Microbiology 2022Quote: ... protein mass fingerprints were obtained using a 5800 MALDI-TOF/TOF mass spectrometer (AB Sciex, Framingham, MA, USA), with detection in the linear middle mass in the range from 5000 to 20 000 Da ...
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bioRxiv - Physiology 2020Quote: ... Here the raw data was searched against the mouse Swissprot database (release October 2017) using the paragon algorithm on Protein-Pilot (Version 5.0.1, AB SCIEX). A total of 33847 proteins were searched ...
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bioRxiv - Molecular Biology 2023Quote: ... only proteins identified with a confidence score (FDR<0.01) and a total protein score >1.0 were retained according to the analysis with ProteinPilot (Sciex). For each protein of interest ...
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bioRxiv - Biochemistry 2020Quote: ... and analytical 75 μm x 150 Atlantis DB C18, 3 μm, 300 Å columns (Water, Milford, MA) interfaced with 6600 TripleTOF (Sciex, Framingham, MA). A 3 min trapping step using 2% ACN ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were analyzed by nanoLC-MS/MS on a Sciex 5600 quadrupole-TOF system and identified using mus musculus or the homo sapien databases with the Protein Pilot (ver 5.0, rev 4769) program (Sciex) as described previously42.
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bioRxiv - Biochemistry 2019Quote: The raw files in the format of “.wiff” containing the spectra of MS and MS/MS were submitted to Protein Pilot v4.0 software (AB Sciex) for protein identification and relative quantification ...
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bioRxiv - Biochemistry 2022Quote: ... Confidence of quantitation for differentially expressed proteins was analyzed with the ProteinPilot Descriptive Statistics Template (PDST) (beta v3.07p, AB SCIEX). Gene ontology (GO ...
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bioRxiv - Biochemistry 2019Quote: ... The results from ProteinPilot were used as an ion library to measure the abundance of peptides and proteins using PeakView 2.1 (SCIEX), with settings ...
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bioRxiv - Systems Biology 2020Quote: ... Tryptic peptides were generated from 8 µg of undepleted plasma proteins and separated on a NanoLC 425 System (SCIEX). 5 µl/min flow was used with trap-elute setting using a ChromXP C18 trap column 0.5 x 10 mm ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... which was used as a sequence database to compare to mass spectral data using the Paragon algorithm in Protein Pilot 2.2 software (AB SCIEX). We report only peptides for which more than two tryptic fragments were detected with >95% confidence ...
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bioRxiv - Neuroscience 2022Quote: 100 μg of protein of each sample was dissolved in a dissolution buffer (AB Sciex, Foster City, CA, USA). After being reduced ...
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bioRxiv - Microbiology 2024Quote: ... coupled to nano-LC system was used to acquire samples and the results were analysed using Protein pilot software 5.0.1 (SCIEX, USA) using the Paragon algorithm ...
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bioRxiv - Cancer Biology 2024Quote: The peptide data obtained from 6600+ TripleTOFTM was analyzed with ProteinPilotTM 3.0 software using the Paragon protein database search algorithm (AB Sciex). IDA data files from ProteinPilotTM and SWAT MS from SWAT MicroApp 2.0 were subjected to isotope pattern matched peak mining using the Extracted Ion Chromatogram (XIC ...
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bioRxiv - Genomics 2021Quote: ... Trypsin digested spots were processed to obtain the protein tags by MS analysis on Hybrid Quadrupole TOF mass spectrometer (API QSTAR PULSAR i, PE SCIEX).
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bioRxiv - Molecular Biology 2021Quote: ... and reconstituted in 6 ul of 0.1% formic acid and peptides were sequenced buy nanoscale liquid chromatography coupled to tandem mass spectrometry (nano LC-MS/MS) and searched using the Protein Pilot program (Sciex).
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Molecular Basis of Sex Difference in Neuroprotection induced by Hypoxia Preconditioning in ZebrafishbioRxiv - Neuroscience 2020Quote: Prior to trypsin digestion all the protein samples were gone through reduction and cysteine blocking using the reagents provided with iTRAQ 4-plex kit (AB SCIEX). Digestion and labelling of proteins were done according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... we used a sperm-specific spectral database obtained through a Data Dependant Acquisition (DDA) proteomic analysis performed on proteins extracted from spermatozoa in different conditions using a TripleTof 6600 mass spectrometer (Sciex).
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bioRxiv - Molecular Biology 2021Quote: ... Acquired spectra were searched against Mycobacterium tuberculosis from NCBInr database using Mascot search algorithm (version 2.0; Matrix Science, Boston, Massachusetts) and protein pilot software (SCIEX, USA) for protein identification (65 ...
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bioRxiv - Cell Biology 2022Quote: ... All MS/MS spectra were separated against the Mus musculus (mouse) proteome data set (UP000000589) at the Uniplot using Protein pilot software (SCIEX).
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bioRxiv - Neuroscience 2022Quote: ... Maximum of twenty precursors with minimum signal: noise ratio was selected from each spectrum for MS/MS analysis and identified using Protein Pilot (AB Sciex). The corresponding proteins against each spectrum showing high interaction with UBA52 were observed using MASCOT and NCBInr database.
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bioRxiv - Cancer Biology 2023Quote: ... IDA runs from MDA-468PC and MDA-468 PDTP-P were pooled together to generate an ion library and for analysis in Protein pilot software v4.5 (Sciex U.S.A) to obtain protein identities ...
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bioRxiv - Neuroscience 2023Quote: Unknown protein identification was performed by injecting 9 µL of the peptide digest onto a 5600 TripleTOF mass spectrometry (AB Sciex) with a microflow LC (Eksigent) ...
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bioRxiv - Systems Biology 2019Quote: Mass spectra were obtained for each prepared protein sample by using three instruments: (i) a TripleTOF 5600+ mass spectrometer with a NanoSpray III ion source (Sciex, USA) coupled to a NanoLC Ultra 2D+ nano-HPLC system (Eksigent ...
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bioRxiv - Plant Biology 2022Quote: A specific ion-library of the precursor masses and fragment ions was created by combining all files from the DDA experiments in one protein identification search using the ProteinPilot™ software (v5.0, Sciex®). The paragon method parameters were the following ...
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bioRxiv - Microbiology 2022Quote: ... 30 μL of the generated tryptic peptides of the microbial proteins were injected into a SCIEX 6600 triple TOF mass spectrometer (AB Sciex, Australia). The proteins were identified by comparing the peptide sequences against UniProt database (Uniprot.org) ...
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bioRxiv - Neuroscience 2022Quote: ... Fifty micrograms of protein from each sample were used for iTRAQ labeling according to the manufacturer’s instructions (iTRAQ Reagents – 8plex, SCIEX Cat# 4390811) and then fractionated into five fractions by cation exchange (Cation Exchange Buffer Pack ...
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bioRxiv - Cell Biology 2019Quote: ... database searching, and protein identification were performed as previously described (13, 43) using ProteinPilot software version 5.0 (AB Sciex, Foster City, CA) and the most recent version (v5.5 ...
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bioRxiv - Cancer Biology 2021Quote: ... 2.5 μg of each sample was run on the nanoLC-MS/MS in DDA mode and the combined DDA runs were searched using Protein Pilot (SCIEX, AB Sciex Pte. Ltd, USA) to create the protein spectral library ...
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bioRxiv - Biochemistry 2021Quote: ... while the parameter for peptides per protein was set to 100 in the software SWATH® Acquisition MicroApp 2.0 in PeakView® version 1.2 (AB Sciex, Foster City, CA, USA). The obtained protein peak areas were exported to Markerview™ version 1.3 (AB Sciex ...
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bioRxiv - Cancer Biology 2021Quote: ... 2.5 μg of each sample was run on the nanoLC-MS/MS in DDA mode and the combined DDA runs were searched using Protein Pilot (SCIEX, AB Sciex Pte. Ltd, USA) to create the protein spectral library ...
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bioRxiv - Molecular Biology 2023Quote: ... using a micro electrode (1–10 µL/min; SCIEX). The solvent system consisted of 0.1% FA in water (solvent A ...
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bioRxiv - Pathology 2020Quote: ... The compound dependent parameters for each MRM transition of analytes are given in Supplement Table.1 and the data was collected using the Analyst 1.6.2 Software (AB Sciex).
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bioRxiv - Physiology 2024Quote: ... and quantitative analysis of the data was carried out by least squares regression of the peak area ratio of the steroid to the corresponding internal standard with equal or 1/x weighting using MultiQuant software v3.0.3 (AB Sciex).
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bioRxiv - Molecular Biology 2022Quote: ... Data quality was ensured by: 1) tuning the DMS compensation voltages using a set of lipid standards (SCIEX, 5040141) after each cleaning ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were lyophilized and re-dissolved in 100 µL 1% formic acid before LC-MS/MS analysis (AB Sciex-API2000) and quantification (AB Sciex-MultiQuant) ...
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bioRxiv - Biophysics 2020Quote: Molecular weight of the purified Sub16 pro was determined by spotting 1 μl of the purified sample with the matrix (sinapic acid) and analyzed on MALDI-TOF/TOF 5800 (AB Sciex) using linear mode ...
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bioRxiv - Biophysics 2020Quote: ... resuspended in 100 mM HEPES pH 7.0 and 1 μl of the sample was spotted with matrix (sinapic acid) and analyzed on MALDI-TOF/TOF 5800 (AB Sciex) as described for the purified protein.
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bioRxiv - Cancer Biology 2022Quote: ... The method uses 100 windows of variable Da effective isolation width with a 1 Da overlap as specified by Sciex. Each window has a dwell time of 150 ms to cover the mass range of 400-1250 m/z in TOF-MS mode and MS/MS data is acquired over a range of 230-1800 m/z with high sensitivity setting and a dwell time of 35 ms ...