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Citations for Quantifoil Micro Tools :
1 - 50 of 86 citations for Insulin like growth factor binding protein 6 IGFBP 6 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: To provide extra support for cell growth 5-6 nm of carbon was evaporated onto Quantifoil Au 200 R2/1 or R2/2 grids (Quantifoil Micro Tools GmbH). Grids were then glow discharged and incubated in supplemented RPMI-1640 overnight in the cell incubator ...
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bioRxiv - Biophysics 2020Quote: ... 3.5 µl of purified channel proteins at ∼6 mg/ml were pipetted onto glow-discharged copper Quantifoil R2/2 holey carbon grids (Quantifoil). Grids were blotted for 2 s at ∼100% humidity and flash frozen in liquid ethane ...
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bioRxiv - Biochemistry 2023Quote: ... Four microliters of purified Cx36 proteins (4-6 mg/ml) were applied onto a negatively glow-discharged holey carbon grid (Quantifoil R1.2/1.3 Cu 300 mesh). For samples involving Cx36-WT ...
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bioRxiv - Biophysics 2020Quote: ... This sample was used for EM dataset 6: the purified sample was applied to Cu 300 R1.2/1.3 holey carbon grids (Quantifoil) coated with a ∼7 nm homemade carbon film ...
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bioRxiv - Biophysics 2023Quote: ... Aliquots of 4 μl of protein samples (0.3 mg ml-1 or ∼6 μM) were applied to glow-discharged R1.2/1.3 200 Cu mesh grids (Quantifoil). After 30 s ...
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bioRxiv - Neuroscience 2023Quote: ... Double application of 3 µL protein (∼0.6 mg/mL) SYM2081 complex was carried out on glow-discharged gold grids (R 0.6/1, 300 mesh, Quantifoil). The grids were blotted for 4.5 s and 3.5 s ...
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bioRxiv - Cell Biology 2021Quote: ... Aggregation was stimulated with TRAP-6 (25μM) in a 96-well plate (15 minutes, 750rpm, 37°C; BioShake IQ, Quantifoil Instruments) and fixed with 1% formalin (Sigma) ...
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bioRxiv - Biophysics 2022Quote: 3.5 μl of protein samples at 4-6 mg/ml were applied to glow-discharged QF R1.2/1.3 300 mesh gold grids (Quantifoil, Großlöbichau, Germany). Grids were blotted for 3 s at 4°C and 100% humidity and plunge-frozen into liquid ethane using FEI Mark IV Vitrobot (FEI ...
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bioRxiv - Biophysics 2019Quote: 3 µL of purified aldolase (1.6 mg/mL) or apoferritin (5 mg/mL) was dispensed on UltrAuFoil R1.2/1.3 300-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly plasma cleaned for six seconds at 15Watts (75% nitrogen/25% oxygen atmosphere ...
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bioRxiv - Cell Biology 2022Quote: The cryo-EM grid was prepared by applying 3 μl of the protein samples (4 – 6 mg/ml) to glow-discharged Quantifoil R1.2/1.3 300-mesh gold holey carbon grids (Quantifoil, Micro Tools GmbH, Germany). Grids were blotted for 4.0 seconds under 100% humidity at 4°C before being plunged into the liquid ethane using a Mark IV Vitrobot (FEI) ...
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bioRxiv - Biophysics 2021Quote: ... 3 μl of purified ghrelin-bound complex at 13 mg/ml and GHRP-6-bound ghrelin receptor complex at 8 mg/ml were applied individually onto a glow-discharged holey carbon grid (Quantifoil, Au300 R1.2/1.3) in a Vitrobot chamber (FEI Vitrobot Mark IV) ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µl of MHAS2168-Mb_H2 complex (9.4 mg/ml and 6 mg/ml) were applied to glow-discharged (45 s) holey carbon grids (Quantifoil R1.2/1.3 Au 200 mesh) and a Grid Plunger GP2 (Leica ...
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bioRxiv - Biophysics 2019Quote: An aliquot of 4 μl of 6 μM Ac-E46K α-syn fibril solution was applied to a glow-discharged holey carbon grid (Quantifoil R1.2/1.3, 300 mesh), blotted for 6.5 s ...
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bioRxiv - Biophysics 2020Quote: ... 2 μL of a 1 in 6 dilution of the batch crystallisation was applied to the carbon side of glow discharged R2/2 Quantifoil™ grids (Quantifoil Micro Tools, Jena, Germany). Excess liquid was removed by blotting for 4-6 s with a Vitrobot (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: Aliquots of 3.5 μL SbtA-SbtB (ΔT-loop) samples (∼6 mg/mL) were applied to the glow-discharged grids (Quantifoil holey carbon Cu R1.2/1.3 grid). Grids were blotted for 4.5 sec and plunge-frozen in liquid ethane vitrified by liquid nitrogen using Vitrobot Mark IV (FEI Company ...
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bioRxiv - Biochemistry 2024Quote: ... or calpain-3-titin/I81-I83 (2mg/mL) from Superose 6 increase 10/300GL column chromatography was loaded onto glow-discharged holey carbon grids (Quantifoil 1.2/1.3 + 2 nm Carbon). The grids were blotted with filter paper for 2.5 secs at 22 °C and then immediately plunged into liquid ethane cooled by liquid nitrogen using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... An aliquot of 4 μL protein sample of Spike protein was applied onto a glow-discharged 300 mesh grid (Quantifoil Au R1.2/1.3) and GraFuture™-RGO grid which was supported with a thin layer of RGO (reduced graphene oxide) ...
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bioRxiv - Biophysics 2020Quote: ... The protein was spotted onto UltraAuFoil 1.2/1.3 gold grids (Quantifoil) plasma cleaned using an Ar/O2 gas mix ...
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bioRxiv - Biophysics 2020Quote: ... Protein was frozen on R 1.2/1.3 grids with 300 Au mesh (Quantifoil, Germany). This dataset was collected at UCSF on the Titan Krios 2 ...
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bioRxiv - Biophysics 2020Quote: The purified N protein was prepared on UltraAuFoil R1.2/1.3 gold support grids (Quantifoil). 3 µl of sample was applied to glow-discharged grids ...
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bioRxiv - Biophysics 2023Quote: ... by applying protein to glow-discharged QuantiFoil Au R1.2/1.3 200-mesh grids (Quantifoil), blotting for 3.0 s under 100% humidity at 4°C ...
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bioRxiv - Immunology 2021Quote: The purified and cross-linked recombinant NLRP3 protein was loaded onto glow-discharged EM-grids (Quantifoil, R2/1 Cu-covered with homemade ultrathin carbon support film) ...
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bioRxiv - Biophysics 2020Quote: ... The protein was then blotted onto Cu 300 mesh Quantifoil 1.2/1.3 grids (Quantifoil Micro Tools) two times with 3.5 μl sample each time ...
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bioRxiv - Plant Biology 2023Quote: ... 3 μl protein was applied to glow-discharged carbon grids (Quantifoil Cu R1.2/1.3 300 mesh). Subsequently ...
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bioRxiv - Microbiology 2021Quote: ... 3μL of protein was deposited on a holey copper grids (QUANTIFOIL R 1.2/1.3, 200 mesh, EMS) which had been glow-discharged for 30s at 15 mA (Tedpella Inc) ...
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bioRxiv - Biophysics 2023Quote: 4 μL purified protein was placed on the holy-carbon grids (Quantifoil Au R1.2/1.3, 300 mesh), which were glow-discharged for 30 s ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μl diluted protein sample was put on freshly glow-discharged Quantifoil R1.2/1.3 Cu grid (Quantifoil Micro Tools GmbH ...
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bioRxiv - Biophysics 2021Quote: ... 2.0 μL of the protein sample was applied to 300 mesh Cu Quantifoil 1.2/1.3 grids (Quantifoil Microtools) that were glow discharged for 20 seconds at 25 mA ...
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bioRxiv - Biophysics 2020Quote: ... Aliquots (3.5 μl) of protein solution were loaded onto glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3), which were blotted for 3.5 s and immersed in liquid ethane cooled by liquid nitrogen using Vitrobot (Mark IV ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μl of protein sample was applied to holey carbon grids (Quantifoil, R 0.6/1 Cu 200 mesh) which were H2/O2 glow-discharged for 30 s ...
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bioRxiv - Plant Biology 2021Quote: ... 2.5 μl L7TIR protein (1mg/ml) was applied to holey carbon grids (Quantifoil Au 1.2/1.3, 300 mesh) glow-discharged for 30 s at high level in Harrick Plasma after 2 min evacuation ...
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bioRxiv - Immunology 2021Quote: ... Then 3 μl of the protein mixture was applied to a Quantifoil-Cu-2/1-3C grid (Quantifoil) pretreated by glow-discharging at 15 mA for 1 min ...
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bioRxiv - Immunology 2022Quote: ... 3 μL of the DCFHP proteins were applied onto a glow-discharged Quantifoil R 1.2/1.3 Cu 300-mesh grid (Quantifoil). The grids were blotted for 2 sec at 20°C and 100% humidity and rapidly cryocooled in liquid ethane using a Vitrobot Mark IV instrument (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... 3μL of protein was deposited on holey carbon copper grids (QUANTIFOIL R 1.2/1.3-2nm, 300 mesh, EMS) which had been glow-discharged for 20s at 15 mA using PELCO easiGlow (TedPella Inc) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 3 μl of the protein complex was applied onto 300 mesh R1.2/1.3 UltrAuFoil Holey gold support films (Quantifoil). For cryo-EM grids preparation of DHA-FFA4-miniGq complex ...
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bioRxiv - Biophysics 2021Quote: ... aliquots (3 μL) of the concentrated protein were placed on glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3) and blotted for 5 s ...
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bioRxiv - Biophysics 2022Quote: Cryo-EM grids were prepared by applying 3 μL of protein to a glow-discharged QuantiAuFoil R1.2/1.3 300-mesh grid (Quantifoil, Germany) and blotted for 2.5 to 4 s under 100% humidity at 4 °C before plunging into liquid ethane using a Mark IV Vitrobot (FEI).
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bioRxiv - Biophysics 2019Quote: ... 20 nL of the 25 nL eluate containing the purified protein was primed on a 400-mesh copper grid covered with a holey carbon film (R1.2/1.3, Quantifoil, Germany) that was glow discharged for 45 s in air plasma immediately before use ...
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bioRxiv - Biophysics 2020Quote: Cryo-EM grids were prepared by applying freshly purified channel protein (~3.5 μl) to glow-discharged copper Quantifoil R2/2 holey carbon grids (Quantifoil), which were then blotted for 2 s with ~100% humidity and flash frozen in liquid ethane using FEI Vitrobot Mark IV (FEI) ...
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bioRxiv - Bioengineering 2020Quote: ... Aliquots (3.3 μL) of the protein complex were placed on glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3). The grids were blotted for 2.5 s or 3.0 s and flash-frozen in liquid ethane cooled by liquid nitrogen with Vitrobot (Mark IV ...
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bioRxiv - Microbiology 2023Quote: ... 3 μL of 3 mg/mL of protein was added to glow discharged UltrAuFoil holey gold grid (Quantifoil GmbH) using a Vitrobot Mark IV (Thermofisher ...
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bioRxiv - Biophysics 2022Quote: Eleven cryoEM grids were prepared by two people by adding 3 μL of mouse apoferritin (mApof, from Dr. Kikkawa’s lab) solution (8 mg/mL) to UltrAuFoil 1.2/1.3 grids (Quantifoil, Jena, Germany) immediately after plasma cleaning (Gatan Solarus II plasma cleaner ...
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bioRxiv - Biochemistry 2021Quote: ... 3.5 μl of the freshly purified sample from the peak fraction in DDM at ~2.0 mg/ml for the B.1.1.7 protein or ~1.5 mg/ml for the B.1.351 protein was applied to a 1.2/1.3 Quantifoil grid (Quantifoil Micro Tools GmbH), which had been glow discharged with a PELCO easiGlow™ Glow Discharge Cleaning system (Ted Pella ...
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bioRxiv - Biophysics 2021Quote: 3μL of inactive or active CaSR protein was applied to glow-discharged holey carbon 300 mesh grids (Quantifoil Au R12/1.3, Quantifoil MicroTools), respectively ...
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bioRxiv - Biochemistry 2022Quote: ... 2–2.5 µl of protein solution was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3 Au 200 mesh). The endogenous samples were applied onto grids containing an additional thin layer of continuous carbon support (Quantifoil R1.2/1.3 Cu 200 mesh + 2 nm C ...
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bioRxiv - Microbiology 2022Quote: ... the purified full-length BA.2 spike protein at 4.6 mg/ml in DDM was applied to a 1.2/1.3 Quantifoil gold grid (Quantifoil Micro Tools GmbH), which had been glow discharged with a PELCO easiGlow™ Glow Discharge Cleaning system (Ted Pella ...
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bioRxiv - Microbiology 2022Quote: ... mixed with 10 nm protein A-coated colloidal gold (Aurion) and applied onto a glow-discharged EM grid (200 mesh, R 2/1, Quantifoil) prior to plunge-freezing with a Leica EM GP2 automatic plunge-freezer.
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bioRxiv - Evolutionary Biology 2022Quote: ... The proteins were incubated at 37°C and 200 rpms for 10 minutes on a microplate shaker (BioShake iQ; Quantifoil Instruments ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μl of protein at a concentration of approximately 10 mg ml−1 were loaded onto glow-discharged holey carbon grids (Quantifoil Au 300 mesh R1.2/1.3 or Quantifoil Cu/Rh 300 mesh R1.2/1.3) ...
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bioRxiv - Biophysics 2023Quote: 3.5 µL of protein or lipid solution was applied onto a freshly glow-discharged carbon-coated copper mesh grid (Quantifoil). After 1 min ...