Labshake search
Citations for Quantifoil Micro Tools :
351 - 400 of 707 citations for 6 Quinoxalinecarboxylicacid 1 2 3 4 tetrahydro 2 oxo methyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... A 3 µl volume of the Kif5B suspension was first deposited at the surface of a glow-discharged holey carbon grid (Quantifoil R2/2, Cu200) in the temperature (23 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Grids were prepared by applying 3.5 μLs of the Z7757 complex or 2 μLs of the Lisuride complex to glow discharged UltrAuFoil holey gold grids (Quantifoil, Au300-R1.2/1.3). The girds containing Z7757 were then vitrified by plunge freezing into a 60/40 mixure of liquid ethane/propane using a Vitrobot mark IV (FEI ...
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bioRxiv - Biochemistry 2021Quote: ... 3 microlitres of undiluted eluate and of eluate which was diluted 1:2 or 1:4 in buffer A200 were blotted onto holey carbon R 1.2/1.3 copper 300 mesh grids (Quantifoil) and vitrified in liquid ethane using a Vitrobot MarkIV device (Thermofisher) ...
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bioRxiv - Biochemistry 2024Quote: ... 4 uL of the concentrated sample was applied to freshly glow-discharged R2/1 holey carbon grids (Quantifoil), blotted with blot force of 5 for 5 s using a Vitrobot Mark IV (Thermo Fisher ...
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bioRxiv - Molecular Biology 2020Quote: ... at 13 °C and 100% humidity: 3 μl of sample were applied onto a freshly glow-discharged copper R2/1 300 mesh grid (Quantifoil), blotted for 8 s on both sides with blotting force −20 and plunge-frozen in liquid ethane.
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bioRxiv - Biochemistry 2020Quote: ... A volume of 3 μL of the sample was applied to a glow-discharged grid (Quantifoil R2/1 300 mesh) at 25 °C and 90 % humidity ...
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bioRxiv - Cell Biology 2021Quote: ... 3 µl of the samples at 1 µg/µl was applied to UltrAUfoil R1.2/1.3 grids (Quantifoil Micro Tools GmbH). Grids were glow-discharged for 30 sec at 15 mA prior to sample application (PELCO easiGlow™ ...
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bioRxiv - Biochemistry 2022Quote: 2.8 µl of F-actin sample (3 – 16 µM) was pipetted onto a glow-discharged R2/1 Cu 300 mesh holey-carbon grid (Quantifoil). After incubating for 1 – 2 seconds ...
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bioRxiv - Biophysics 2020Quote: ... 2.5 μl samples of GDN-purified proteins at a concentration of 1.5-3 mg ml−1 were applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3 or R0.6/1 Au 200 mesh). Excess liquid was removed in a controlled environment (4°C and 100% relative humidity ...
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bioRxiv - Biophysics 2022Quote: UltraAUfoil R1.2/3 (Quantifoil Micro Tools GmbH) grids were glow-discharged on both sides for 30 s at 15 mA using a Pelco Easiglow ...
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bioRxiv - Cell Biology 2020Quote: 4 µl of the cultured yeast cells (OD600 of 0.4) were added to a glow-discharged (30 seconds at -30 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools GmbH, Jena, Germany), then the grid was rapidly plunge frozen in liquid ethane using a homemade plunge freezer and stored in liquid nitrogen until used ...
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bioRxiv - Biochemistry 2020Quote: ... 2.5 μL aliquots of purified Dnf1p–Lem3p or Dnf2p–Lem3p at a concentration of about 1.5 mg/mL were placed on glow-discharged holey carbon grids (Quantifoil Au R2/2, 300 mesh) and were flash-frozen in liquid ethane using a FEI Vitrobot Mark IV ...
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bioRxiv - Cell Biology 2022Quote: ... 4 µL of the mixture were pipetted onto a copper EM grid with holey carbon film (R2/2, 200 mesh, Quantifoil Micro Tools GmbH, Q43486) that had been freshly glow-discharged for 30 s at -30 mAmp ...
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bioRxiv - Molecular Biology 2021Quote: Purified mtLSU or 55S mitoribosome samples (4 μL) were applied to freshly glow discharged R 3.5/1 holey carbon grids (Quantifoil) that were precoated with a 2-3 nm carbon layer using a Leica EM ACE600 coater ...
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bioRxiv - Molecular Biology 2019Quote: ... Cryo-EM grids were prepared by application of 3.5 µl of the sample containing purified Ccr4-Not:ribosome complexes onto R3/3 copper grids with 3 nm continuous carbon support (Quantifoil) and vitrification in liquid ethane using a Vitrobot Mark IV (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... 3 μL of 3 mg/mL of protein was added to glow discharged UltrAuFoil holey gold grid (Quantifoil GmbH) using a Vitrobot Mark IV (Thermofisher ...
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bioRxiv - Biophysics 2024Quote: Approximately 3□μL of the sample solution were put on newly glow-discharged TEM grids (Quantifoil R2/1, Cu, mesh 200) and plunge-frozen in liquid ethane using the Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: RNA granule preparations were diluted with RNA Granule buffer to an optical density A260 = 2 U/mL aliquots of the dilution deposited on glow-discharged Quantifoil grids (Quantifoil 1.2/1.3, 300 mesh copper) pre-coated with a continuous layer of 3 nm carbon ...
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bioRxiv - Molecular Biology 2022Quote: 3 μL of ~120 nM mitoribosome was applied onto a glow-discharged (20 mA for 30 sec) holey-carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ~3 nm thickness ...
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bioRxiv - Cell Biology 2019Quote: ... 4 µl of the solution was applied to a glow-discharged (30 s at −35 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools GmbH, Jena, Germany). After removing excess liquid by blotting the grid from the back side with Whatman filter paper No ...
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bioRxiv - Microbiology 2019Quote: ... Four μl of this mixture was applied onto freshly glow-discharged copper R2/2 of th0 Quantifoil holey carbon grids (Quantifoil Micro Tools GmbH, Jena, Germany). Using an FEI Vitrobot Mark IV ...
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bioRxiv - Biochemistry 2022Quote: ... The endogenous samples were applied onto grids containing an additional thin layer of continuous carbon support (Quantifoil R1.2/1.3 Cu 200 mesh + 2 nm C) and incubated for 30 sec before blotting and freezing ...
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bioRxiv - Biochemistry 2023Quote: 3 μL of ∼120 nM mitoribosome was applied onto a glow-discharged (20 mA for 30 sec) holey- carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ∼3 nm thickness ...
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bioRxiv - Biophysics 2023Quote: ... 4 μl of the extracellular parasites were pipetted onto a glow-discharged (30 seconds at −30 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools GmbH, Jena, Germany). Samples were back-blotted with a Whatman filter paper (grade 1 ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μL of ∼120 nM mitoribosome was applied onto a glow-discharged (20 mA for 30 sec) holey-carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ∼3 nm thickness ...
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bioRxiv - Biophysics 2021Quote: ... Log phase cultures were centrifuged at 2000 g for 2 minutes to concentrate the cells 10 times and 4 μl was directly applied to a glow-discharged holey carbon R2/1 Cu 200 mesh grid (Quantifoil). Plunge-freezing was performed using a VitroBot Mark 4 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... trehalose solution to mitigate crystalline ice formation and 4 μL of this mixture immediately applied to a R2/1 Cu 200 grid (Quantifoil), which had been glow-discharged for 1 min at 0.19 mbar and 20 mA in a PELCO easiGlow device shortly before use ...
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bioRxiv - Biophysics 2023Quote: ... 4 µL fresh sample (0.48 mg/mL) was applied on glow-discharged Au holey carbon grids (Quantifoil R 0.6/1) and the following operation were the same as describe above for preparing the sample of gMCM8/9 complex.
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bioRxiv - Biophysics 2023Quote: ... 200 mesh R1/4 or R2/4 Quantifoil holy carbon gold grids (Quantifoil®, Grossloebichau/Jena Germany). were glow discharged in Pelco easiGlow (Ted Pella ...
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bioRxiv - Biophysics 2023Quote: ... 3 μl of medium containing one isolated muscle fibre was placed on the glow-discharged grid (Quantifoil R2/1 Au 200 mesh). After incubation for 60 seconds at 37 °C ...
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bioRxiv - Biophysics 2019Quote: ... Approximately 5 µL of sample (diluted if necessary) was placed on a copper grid (Quantifoil® S7/2 Cu 400 mesh, holey carbon films, Quantifoil Micro Tools GmbH, Jena, Germany) and any excess liquid was absorbed by a filter paper ...
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bioRxiv - Biochemistry 2022Quote: The samples were frozen on copper or gold grids with holey carbon film coated with a 2 nm continuous carbon film (Quantifoil R1.2/1.3 ultrathin carbon, 300 mesh) freshly glow discharged for 20 s at 15 mA (PELCO easiGlow) ...
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bioRxiv - Neuroscience 2023Quote: ... 3.5 μL of CopiaGag capsid protein sample was applied to a 300-mesh copper grid with continuous 2 nm carbon film (Electron Microscopy Sciences, QUANTIFOIL® C2-C15nCu30-50) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
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bioRxiv - Molecular Biology 2021Quote: ... cells in suspension (4 μl of ~1,000 cells per μl) were blotted onto R2/1 carbon-coated 200-mesh copper grids (Quantifoil Micro Tools) and plunge frozen in a liquid ethane/propane mixture ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of the sample was applied to a glow-discharged R2/1 300 mesh holey carbon copper grid (Quantifoil Micro Tools GmbH) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of diluted sample was applied to a glow-discharged (R2/1 300 mesh holey carbon copper grid (Quantifoil Micro Tools GmbH) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μL of sample (10 μM EGFP in PBS) was deposited onto glow-discharged grids (Electron Microscopy Science and Quantifoil – see Table 1), prior to blotting the grids from the sample-side for 3 seconds.
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bioRxiv - Molecular Biology 2024Quote: ... about 0.9 mg/ml for DSS1–INTAC–PEC complex) was applied onto a glow-discharged holey carbon grid (Quantifoil Au, R1.2/1.3 or R2/2, 300 mesh). After blotting for 3 to 6 s ...
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bioRxiv - Cell Biology 2024Quote: ... the sample was gently resuspended and 4 µl was transferred to a glow-discharged holey carbon R2/1 copper grid (Quantifoil Micro Tools) suspended in the chamber of a Vitrobot (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: KRASGTPase/BRAFWT/MEK1SASA/14-3-3 complex in SEC buffer was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, 400 mesh) and vitrified using a Leica EM GP ...
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bioRxiv - Microbiology 2024Quote: ... Sample volumes of 3.5 µl (8 OD260 per ml) were applied to grids (Quantifoil, Cu, 300 mesh, R3/3 with 3 nm carbon) which had been freshly glow-discharged using a GloQube (Quorum Technologies ...
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bioRxiv - Biochemistry 2019Quote: 2.5 µl of freshly purified protein at a concentration of 3.3 mg/ml when solubilized in DDMand at about 2 mg/ml when reconstituted in nanodiscs were applied on holey-carbon cryo-EM grids (Quantifoil Au R1.2/1.3, 200, 300 and 400 mesh), which were prior glow-discharged at 5 mA for 30 s ...
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bioRxiv - Biochemistry 2022Quote: Freshly prepared ribosome–Sec61–KZR-8445 complex at 300–500 nM concentration was applied on glow discharged holey-carbon grids (Quantifoil, R1.2/1.3 with a 2-nm carbon film) and flash frozen with an automated plunge freezer (Leica microsystems) ...
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bioRxiv - Biophysics 2021Quote: ... A suspension containing a mixture of 10 nm protein A coated colloidal gold (Aurion) and Ebola VLPs was applied with a volume of 3 µl on a glow discharged EM grid (Quantifoil, 200 Mesh, R2/1). The grid was blotted using No.1 Whatman paper for 3 sec from the back-side in a chamber with controlled humidity set to 70% and the temperature set to 24°C.
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bioRxiv - Molecular Biology 2023Quote: Freshly purified ZorAB sample was concentrated to 2-3 mg/mL and 2.7 μL protein was applied onto the glow-discharged (30 s, 5 mA) grids (Quantifoil R0.6/1 300 mesh Au) and plunge-frozen into liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Cell Biology 2020Quote: ... or holey SiO2 R1/4 TEM grids (Quantifoil) to allow for more robust correlation and cells were plunge frozen on a Vitrobot Mark IV (FEI ...
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bioRxiv - Cell Biology 2022Quote: ... Grids (200 Mesh Cu SiO2 R1/4, Quantifoil) were plasma cleaned for 30 s before plunging ...
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bioRxiv - Molecular Biology 2021Quote: Copper grids with holey carbon support film (R3/3, Quantifoil) and a 2 nm pre-coated continuous carbon layer on top were glow discharged at 2.2 × 10-1 mbar for 20 s ...
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bioRxiv - Molecular Biology 2023Quote: ... a 4 µl sample of purified complex was applied to glow-discharged R2/1 300 mesh holey carbon copper grids (Quantifoil Micro Tools GmbH), using a conc ...
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bioRxiv - Cell Biology 2023Quote: 4 ul of Nannochloropsis suspensions (4 x 107 cells mL−1) were pipetted onto a 300 mesh gold Quantifoil grid with 2um holes (Quantifoil Micro Tools, GmbH) and manually blotted from the opposite side in a Vitrobot Mark IV (ThermoFisher Scientific ...