Labshake search
Citations for Quantifoil Micro Tools :
1 - 50 of 400 citations for 6 Oxo 5 6 7 8 tetrahydronaphthalene 2 carbonitrile since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: To provide extra support for cell growth 5-6 nm of carbon was evaporated onto Quantifoil Au 200 R2/1 or R2/2 grids (Quantifoil Micro Tools GmbH). Grids were then glow discharged and incubated in supplemented RPMI-1640 overnight in the cell incubator ...
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bioRxiv - Biophysics 2021Quote: ... 3 μl of purified ghrelin-bound complex at 13 mg/ml and GHRP-6-bound ghrelin receptor complex at 8 mg/ml were applied individually onto a glow-discharged holey carbon grid (Quantifoil, Au300 R1.2/1.3) in a Vitrobot chamber (FEI Vitrobot Mark IV) ...
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bioRxiv - Biophysics 2020Quote: ... 3.5 µl of purified channel proteins at ∼6 mg/ml were pipetted onto glow-discharged copper Quantifoil R2/2 holey carbon grids (Quantifoil). Grids were blotted for 2 s at ∼100% humidity and flash frozen in liquid ethane ...
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bioRxiv - Biophysics 2019Quote: 3 µL of purified aldolase (1.6 mg/mL) or apoferritin (5 mg/mL) was dispensed on UltrAuFoil R1.2/1.3 300-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly plasma cleaned for six seconds at 15Watts (75% nitrogen/25% oxygen atmosphere ...
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bioRxiv - Biophysics 2020Quote: ... 2 μL of a 1 in 6 dilution of the batch crystallisation was applied to the carbon side of glow discharged R2/2 Quantifoil™ grids (Quantifoil Micro Tools, Jena, Germany). Excess liquid was removed by blotting for 4-6 s with a Vitrobot (Thermo Fisher ...
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bioRxiv - Biochemistry 2024Quote: ... or calpain-3-titin/I81-I83 (2mg/mL) from Superose 6 increase 10/300GL column chromatography was loaded onto glow-discharged holey carbon grids (Quantifoil 1.2/1.3 + 2 nm Carbon). The grids were blotted with filter paper for 2.5 secs at 22 °C and then immediately plunged into liquid ethane cooled by liquid nitrogen using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2020Quote: ... This sample was used for EM dataset 6: the purified sample was applied to Cu 300 R1.2/1.3 holey carbon grids (Quantifoil) coated with a ∼7 nm homemade carbon film ...
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bioRxiv - Biophysics 2023Quote: ... Aliquots of 4 μl of protein samples (0.3 mg ml-1 or ∼6 μM) were applied to glow-discharged R1.2/1.3 200 Cu mesh grids (Quantifoil). After 30 s ...
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bioRxiv - Neuroscience 2023Quote: ... Double application of 3 µL protein (∼0.6 mg/mL) SYM2081 complex was carried out on glow-discharged gold grids (R 0.6/1, 300 mesh, Quantifoil). The grids were blotted for 4.5 s and 3.5 s ...
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bioRxiv - Cell Biology 2021Quote: ... Aggregation was stimulated with TRAP-6 (25μM) in a 96-well plate (15 minutes, 750rpm, 37°C; BioShake IQ, Quantifoil Instruments) and fixed with 1% formalin (Sigma) ...
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bioRxiv - Biophysics 2022Quote: 3.5 μl of protein samples at 4-6 mg/ml were applied to glow-discharged QF R1.2/1.3 300 mesh gold grids (Quantifoil, Großlöbichau, Germany). Grids were blotted for 3 s at 4°C and 100% humidity and plunge-frozen into liquid ethane using FEI Mark IV Vitrobot (FEI ...
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bioRxiv - Biophysics 2023Quote: ... 0.5 μL L-PEG-L (8 mg/ml) and 0.5 μL Pd(NO3)2 (1 mg/ml) was applied to holey carbon grid (Quantifoil 200 mesh Cu R2/2) which was glow discharged 45 s at 15 mA ...
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bioRxiv - Microbiology 2023Quote: ... This mixture was applied to the front (3.7 µL) and to the back (1.3 µL) of carbon-coated copper grids (R2/2, Cu 200 mesh; Quantifoil) previously glow-discharged (2 mA ...
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bioRxiv - Biophysics 2023Quote: ... HeLa cells were trypsinized and seeded at a density of 0.5 – 0.7 X 105 cells/mL on carbon-coated gold Quantifoil grid (200 mesh, R 2/1, holey SiO2 film; Quantifoil Micro Tools GmbH ...
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bioRxiv - Cell Biology 2022Quote: The cryo-EM grid was prepared by applying 3 μl of the protein samples (4 – 6 mg/ml) to glow-discharged Quantifoil R1.2/1.3 300-mesh gold holey carbon grids (Quantifoil, Micro Tools GmbH, Germany). Grids were blotted for 4.0 seconds under 100% humidity at 4°C before being plunged into the liquid ethane using a Mark IV Vitrobot (FEI) ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µl of MHAS2168-Mb_H2 complex (9.4 mg/ml and 6 mg/ml) were applied to glow-discharged (45 s) holey carbon grids (Quantifoil R1.2/1.3 Au 200 mesh) and a Grid Plunger GP2 (Leica ...
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bioRxiv - Cell Biology 2022Quote: Cells were applied onto galectin-8 coated EM grids with carbon support film (R2/2, Au mesh; Quantifoil, Jena, Germany). After 15-20 min incubation ...
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bioRxiv - Biophysics 2019Quote: An aliquot of 4 μl of 6 μM Ac-E46K α-syn fibril solution was applied to a glow-discharged holey carbon grid (Quantifoil R1.2/1.3, 300 mesh), blotted for 6.5 s ...
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bioRxiv - Microbiology 2023Quote: Aliquots of 3.5 μL SbtA-SbtB (ΔT-loop) samples (∼6 mg/mL) were applied to the glow-discharged grids (Quantifoil holey carbon Cu R1.2/1.3 grid). Grids were blotted for 4.5 sec and plunge-frozen in liquid ethane vitrified by liquid nitrogen using Vitrobot Mark IV (FEI Company ...
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bioRxiv - Biochemistry 2023Quote: ... Four microliters of purified Cx36 proteins (4-6 mg/ml) were applied onto a negatively glow-discharged holey carbon grid (Quantifoil R1.2/1.3 Cu 300 mesh). For samples involving Cx36-WT ...
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bioRxiv - Biochemistry 2023Quote: Farnesylated GBP1 reconstituted on liposomes (as described above) were supplemented with 5 nm colloidal gold and applied on glow-discharged UltrAuFoil R 2/2 gold grids (Quantifoil Micro Tools), flash-frozen in liquid ethane using a Vitrobot Mark II device (FEI) ...
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bioRxiv - Molecular Biology 2022Quote: The purified complex samples (3 μL at 5-8 mg/mL) were applied to glow-discharged holey grids (Quantifoil R1.2/1.3, 300 mesh) and subsequently vitrified using a Vitrobot Mark IV (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: ... 3.5 μL was applied to a glow-discharged Quantifoil copper grid with 2 nm continuous carbon support over holes (Quantifoil R 2/4 400 mesh), blotted ...
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bioRxiv - Molecular Biology 2019Quote: ... 2.8 µl of 0.10 mg/ml of the protein preparation were applied to glow-discharged holey carbon grids (Quantifoil, QF 2/1, 300 mesh) covered with a 2 nm carbon layer ...
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bioRxiv - Microbiology 2022Quote: ... the amoebae (5×105 per well) were seeded onto EM gold finder grids (Au NH2 R2/2, Quantifoil) and incubated for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... and 5 µl of the mixture was placed onto freshly glow discharged holey carbon grids (R 2/1, Quantifoil) for 1 min ...
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bioRxiv - Molecular Biology 2020Quote: ... plain grids (Quantifoil 2/2) were transferred to the microscope ...
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bioRxiv - Biochemistry 2024Quote: ... carbon-coated (Quantifoil R 2/2) 200-mesh copper grids (Electron Microscopy Sciences ...
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bioRxiv - Synthetic Biology 2021Quote: ... 25 mM Tris at pH 8.0 to a concentration of 2 mg/mL to a glow-discharged (1 min at 5 mA) holey carbon grid (Quantifoil Q225-CR1.3). This was followed by plunge-freezing in liquid ethane using an FEI Vitribot Mark IV (100% humidity ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated at 37°C and 5% CO2 for 16h prior to trypsinization and seeding onto 200 mesh gold holey carbon grids (R2/2-2C; Quantifoil Micro Tools), which were placed in 3D printed grid holders [39] ...
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bioRxiv - Cell Biology 2024Quote: ... An aliquot of a 5 μl sample was applied to a glow-discharged holey carbon grid (Quantifoil R2/2, Au, 400 mesh). After waiting for 60 s ...
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bioRxiv - Microbiology 2021Quote: ... 4-7 µl of cells were deposited on R2/1 Cu 200 grids (Quantifoil) that had been glow-discharged for 1 min at 0.19 mbar and 20 mA in a PELCO easiGlow device shortly before use ...
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bioRxiv - Biochemistry 2022Quote: ... holey copper grids (QUANTIFOIL R 2/2). 3.5-μl samples were adsorbed for 1 min at +20 °C ...
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bioRxiv - Microbiology 2020Quote: Holey carbon-coated (R 2/2; Quantifoil) Au-G200F1 grids were analyzed in cryo-conditions by MISTRAL microscope at ALBA synchrotron ...
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bioRxiv - Biophysics 2023Quote: ... Micropatterns covered an area of 8 × 8 squares around the center of the cryo-TEM grids (Quantifoil R1/4 or R1/20, Au 200 mesh, SiO2 film) as previously described31 ...
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bioRxiv - Biophysics 2022Quote: Purified wt or mutant D263A or F55A MVMp capsid samples (5 μl) were applied onto R2/2 300 mesh copper grids (Quantifoil Micro Tools, Germany) and vitrified using a Leica EM CPC cryofixation unit ...
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bioRxiv - Microbiology 2022Quote: ... 5 µL of the mixture was placed onto freshly glow discharged holey carbon grids (Quantifoil™ R 2/1 on 200 copper mesh) for 1 minute ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µl of sample were applied to glow-discharged Quantifoil Au 2/2 holey carbon 200 mesh grids or Ultrafoil Au 2/2 holey gold 200 mesh grids (Quantifoil), and flash frozen in liquid ethane using an FEI Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: A QuantiFoil 300-mesh R 2/2 grid with 2 nm continuous carbon (QuantiFoil Micro Tools GmbH ...
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bioRxiv - Biochemistry 2020Quote: UltrAUfoil 2/2 300 mesh gold grids (Quantifoil) were used for open hole data collection (Dataset 1) ...
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bioRxiv - Biophysics 2020Quote: ... or QUANTIFOIL R 2/2 holey carbon grids (Quantifoil Micro Tools GmbH ...
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bioRxiv - Biophysics 2022Quote: ... holey carbon grid coated with 2 nm ultrathin carbon film (Quantifoil 300-mesh, R 2/2). After waiting for 60 s ...
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bioRxiv - Biophysics 2019Quote: ... samples were prepared on 2/2 holey carbon grids (Quantifoil) and blotted for 6-8 s ...
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bioRxiv - Biochemistry 2021Quote: Holey grids (gold, 200 mesh, r 2/2 by Quantifoil) were glow-discharged for 30 seconds using a Pelco glow discharge system ...
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bioRxiv - Biophysics 2024Quote: Holey carbon grids (Quantifoil Au R 2/2, 200 mesh) were glow-discharged with easiGlow at 20 mA for 45 s ...
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bioRxiv - Biophysics 2022Quote: A volume of 4-7 µl of cells were deposited on R2/1 Cu 200 grids (Quantifoil) that had been glow-discharged for 1 min at 0.19 mbar and 20 mA in a PELCO easiGlow device shortly before use ...
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bioRxiv - Biophysics 2023Quote: ... split onto Poly-D-Lysine coated R 2/2 grids (QUANTIFOIL 100 Holey Carbon Films ...
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bioRxiv - Biophysics 2023Quote: ... glow discharged QUANTIFOIL® copper R 2/2 grid with a 2 nm thin extra carbon layer (QUANTIFOIL, GMBH) was used ...
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bioRxiv - Biochemistry 2021Quote: ... Quantifoil Cu 300 mesh (R 2/2 geometry; Quantifoil Micro Tools GMBH) covered with a thin layer of 3 nm carbon were used for the for the GTPBP5KO sample ...
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bioRxiv - Biochemistry 2022Quote: R2/2 grids (Quantifoil) were covered with a thin layer of continuous carbon (estimated to be 50 Å thick ...