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Citations for Quantifoil Micro Tools :
251 - 300 of 349 citations for 6 Iodo 2 Tetralone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... An aliquot (3 μl) of this mixture was applied to a glow-discharged holey carbon grid (QF R2/2 Cu, QUANTIFOIL), blotted with Whatman® No ...
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bioRxiv - Biochemistry 2022Quote: ... 2–2.5 µl of protein solution was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3 Au 200 mesh). The endogenous samples were applied onto grids containing an additional thin layer of continuous carbon support (Quantifoil R1.2/1.3 Cu 200 mesh + 2 nm C ...
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bioRxiv - Microbiology 2022Quote: ... and 4 µl of the mixture was applied to a glow-discharged holey-carbon copper EM grid (R2/1 or R2/2, Quantifoil). The grid was automatically blotted from the backside for 4-6 s in a Mark IV Vitrobot by using a Teflon sheet on the front pad ...
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bioRxiv - Microbiology 2022Quote: ... mixed with 10 nm protein A-coated colloidal gold (Aurion) and applied onto a glow-discharged EM grid (200 mesh, R 2/1, Quantifoil) prior to plunge-freezing with a Leica EM GP2 automatic plunge-freezer.
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bioRxiv - Cell Biology 2023Quote: Cells were blotted on EM grids (R 2/1 holey carbon 200 films on Cu 200 mesh, Quantifoil Micro Tools). The grids were first glow-discharged and 4 µl of trypanosome wild-type culture (2-4 · 107 cells/ml ...
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bioRxiv - Microbiology 2023Quote: ... and 4 µl of the mixture was applied to a glow-discharged holey-carbon copper EM grid (R2/1 or R2/2, Quantifoil). The grid was automatically backside blotted for 4-6s in a Mark IV Vitrobot (Thermo Fischer Scientific ...
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bioRxiv - Biophysics 2023Quote: ... HeLa cells were trypsinized and seeded at a density of 0.5 – 0.7 X 105 cells/mL on carbon-coated gold Quantifoil grid (200 mesh, R 2/1, holey SiO2 film; Quantifoil Micro Tools GmbH ...
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bioRxiv - Cell Biology 2023Quote: ... An aliquot of 3 ul cell suspension was applied to the glow-discharged holey carbon-coated copper (R 2/1, 200 mesh) (Quantifoil) and blotted for 9 seconds by Leica GP2 (Leica Microsystems) ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples for cryo-ET were deposited onto 300-mesh holey carbon grids (Quantifoil Micro Tools, R2/2 X-103-Cu300) and samples for cryo-SPA were deposited onto 200-mesh holey carbon grids (Quantifoil Micro Tools ...
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bioRxiv - Biochemistry 2023Quote: ... 3 µL of each sample was applied to glow-discharged (PELCO easiGlow, 15 mA, 2 min) holey carbon grid (Quantifoil R1.2/1.3 on gold or copper 200 mesh support) ...
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bioRxiv - Biophysics 2023Quote: A 3 µL aliquot of purified CDTbD623A/D734Aprotein sample at a concentration of 30 µM was applied to a glow discharged QUANTIFOIL® copper R 1.2/1.3 grid with a 2 nm thin extra carbon layer (QUANTIFOIL, GMBH). For calcium depleted CDTb (28 µM) ...
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bioRxiv - Biophysics 2023Quote: ... 3 or 3.5 μl of LRRK2RCKW:inhibitor:E11 DARPin complex were applied to a glow-discharged UltraAuFoil Holey Gold 200 mesh R2/2 grid (Quantifoil, Catalog # Q250AR2A) and incubated in a FEI Vitrobot IV chamber at 4°C and 95% humidity for 20 sec ...
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bioRxiv - Biochemistry 2023Quote: 2.4 µL of sample were applied to a freshly glow discharged 200-mesh Cu + 2 nm C R1.2/1.3 grid (Quantifoil Micro Tools), blotted for 6 s at 100% humidity ...
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bioRxiv - Cell Biology 2024Quote: ... 4 × 104 MDM cells were seeded on glow discharged and UV-light sterilized holey carbon grids (R2/2, Au, 200 mesh, Quantifoil), which were placed in a glass-bottomed ‘microwell’ of 35-mm MatTek dish (MatTek ...
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bioRxiv - Cell Biology 2024Quote: ... 3 µl of cell suspension was applied to a freshly grown discharged holey carbon grid (Quantifoil® #200, R2/2). Grids were immediately blotted from the back side using Whatman filter paper #1 (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... using 3 µL of the samples applied to previously glow discharged R 2/1 Holey Carbon Cu 200 mesh EM grids (Quantifoil). Samples were 10 µM FtsZ with or without ZapD or mZapD at different concentrations specified in each case (0 – 60 µM) ...
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bioRxiv - Microbiology 2019Quote: 3 µl of S-layer suspension were applied to glow-discharged 300 mesh copper Quantifoil grids (R2/2, Quantifoil, Jena, Germany), blotted for 3 – 5 seconds and rapidly injected into liquid ethane using a homemade plunge-freezer ...
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bioRxiv - Biophysics 2019Quote: ... of suspended crystals was loaded on one side of glow-discharged Quantifoil EM grids (R2/2, Cu 300 mesh; QUANTIFOIL Company). The grid was then blotted from the other side and washed twice manually with 4 μL 5% v/v PEG 200 buffer ...
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bioRxiv - Microbiology 2019Quote: ... The EM-grids were glow-discharged 200 mesh copper grids with an extra thick R2/2 carbon film (Quantifoil Micro Tools). 3,5 µl of the sample was applied to the EM grid and blotted at a temperature of 16°C and a humidity between 95-99% ...
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bioRxiv - Biophysics 2019Quote: ... The Netherlands) as fiducial markers and glow-discharged R2/2 Cu 300 mesh holey carbon-coated support grids (Quantifoil, Jena, Germany) were dipped into the solution ...
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bioRxiv - Biophysics 2020Quote: ... samples were stored on ice and applied to the cryo-grids (2 nm precoated Quantifoil R3/3 holey carbon supported grids) at a concentration of 10 μM and vitrified using a Vitrobot Mark IV (FEI) ...
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bioRxiv - Plant Biology 2021Quote: ... 3 µl of suspended cells in culture medium were applied to the front side of glow-discharged R2/2 carbon-coated copper grids (Quantifoil MicroTools), with additional 1 µL cells added to the back side ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of the sample mixture was applied to a freshly glow-discharged copper R2/2 200 grid (Quantifoil Micro Tools), pre-blotted for 30 seconds ...
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bioRxiv - Biophysics 2019Quote: 3.5 ul of sample of doublets (∼4 mg/ml) was applied to a glow-discharged holey carbon grid (Quantifoil R2/2), blotted and plunged into liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Three microliters of the protein solution were applied on a glow-discharged holey carbon grids (200 mesh Quantifoil R2/2 molybdenum), which had been treated with H2 and O2 mixtures in a Solarus plasma cleaner (Gatan ...
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bioRxiv - Cell Biology 2022Quote: ... The IL-11Δ10/IL-11RαD1-D3/gp130D1-D3 complex (0.5 mg/mL) was blotted onto UltrAuFoil grids (R2/2, Quantifoil Micro Tools GmbH) that had been subjected to glow discharge (15 mA for 30 s) ...
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bioRxiv - Microbiology 2023Quote: ... sample were placed onto glow-discharged Quantifoil R2/1 Cu300 holey carbon grids (1 µm spacing, 2 µm holes, copper mesh) (Quantifoil, Germany), blotted for 3.5 s before flash-freezing in liquid ethane using a Vitrobot mark IV (FEI ...
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bioRxiv - Biophysics 2023Quote: ... 3 to 3.5 μl of complex was applied to a glow-discharged UltrAuFoil Holey Gold 200 mesh R2/2 grid (Quantifoil, Catalog # Q250AR2A) and incubated in a FEI Vitrobot IV chamber at 4°C and 95% humidity for 20 sec ...
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bioRxiv - Synthetic Biology 2021Quote: ... 25 mM Tris at pH 8.0 to a concentration of 2 mg/mL to a glow-discharged (1 min at 5 mA) holey carbon grid (Quantifoil Q225-CR1.3). This was followed by plunge-freezing in liquid ethane using an FEI Vitribot Mark IV (100% humidity ...
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bioRxiv - Biophysics 2021Quote: 3 μl of the human sperm sample were applied to glow discharged copper R2/2 200-mesh holey carbon grid (Quantifoil Micro Tools) and plunge frozen in liquid ethane using an automatic plunge freezer (Vitrobot ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated at 37°C and 5% CO2 for 16h prior to trypsinization and seeding onto 200 mesh gold holey carbon grids (R2/2-2C; Quantifoil Micro Tools), which were placed in 3D printed grid holders [39] ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 μL aliquots of the cell suspension were applied to glow-discharged R2/2 200 mesh copper Quantifoil grids (Quantifoil Micro Tools), the sample was pre-blotted for 30 seconds ...
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bioRxiv - Molecular Biology 2021Quote: ... After another 3 min at 37 °C the termination complexes were placed on ice for 15 min and subsequently applied to Quantifoil R2/2 holey carbon grids (Quantifoil Micro Tools) either coated with a thin continuous carbon film or with graphene oxide flakes (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2019Quote: 3.5 μl of sample of fragmented doublets (~4 mg/ml) or the Sarkosyl A-tubule (~500 μg/ml) was applied to a glow-discharged holey carbon grid (Quantifoil R2/2), blotted and plunged into liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2019Quote: 3.5 μl of microtubule fraction sample (∼4 mg/ml) purified from WT Chlamydomonas was applied to glow-discharged holey carbon grids (Quantifoil R2/2), blotted and vitrified in liquid ethane using the Vitrobot Mark IV (FEI Company) ...
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bioRxiv - Cell Biology 2021Quote: ... 4 µl of the solution was applied to a glow-discharged (30s at 35 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools). After removing excess liquid by blotting the grid from the back side with Whatman filter paper for 2 s ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were applied to freshly glow-discharged (Edwards S150B, 45 sec, 30-50 mA, 1,2kV, 10-2 mbar) copper holey carbon grids (Quantifoil, Cu-R1.2/1.3) under 100% humidity ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μl of the mixture was deposited on glow discharged R2/2 Copper 300 mesh holey carbon-coated support grids (Quantifoil, Jena, Germany) along with 1 μl of HEPES pH 10 and 1 μl of hydrogen peroxide 0.9 % ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: A 4 μL solution of co-isolated small particles were applied to glow-discharged copper grids (Quantifoil R2/2, Quantifoil Micro Tools), blotting for 3-seconds at 4 °C with 90% humidity and plunging into liquid ethane using a Lecia EM GP device (Leica Microsystems ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: A 4 μL solution of co-isolated small particles were applied to glow-discharged copper grids (Quantifoil R2/2, Quantifoil Micro Tools), blotting for 3-seconds at 4 °C with 90% humidity and plunging into liquid ethane using a Lecia EM GP device (Leica Microsystems ...
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bioRxiv - Neuroscience 2021Quote: ... 400 mesh Quantifoil R1.2/1.3 grids with empty holes and 400 mesh Quantifoil R1.2/1.3 grids coated with a 2 nm thin carbon layer (Quantifoil Micro Tools GmbH, Germany). The grids were rendered hydrophilic by glow discharge using a Pelco easiGlow glow discharger (Ted Pella Inc. ...
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bioRxiv - Microbiology 2020Quote: ... Cells were concentrated by centrifugation and 3 μL aliquots of the cell suspension are applied to glow-discharged R2/2 200 mesh copper Quanti-foil grids (Quantifoil Micro Tools), the sample was pre-blotted for 30 seconds ...
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bioRxiv - Cell Biology 2020Quote: ... A 5 µl drop of the chemically fixed cell culture was deposited on a glow-discharged Quantifoil 200 mesh R2/2 electron microscopy grid (Quantifoil, Großlöbichau, Germany) pre-coated with a gold bead solution ...
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bioRxiv - Cell Biology 2020Quote: ... 3.5 µL of gel filtration column eluate (∼0.5 mg/ml) was applied to a holey carbon support (Quantifoil 2/1-4Cu-50) at ∼90% relative humidity ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µL aliquots of 0.1 mg/ml cross-linked ternary complexes were applied to freshly glow-discharged R2/2 Quantifoil 200 mesh copper grids (Quantifoil Micro Tools). Grids were blotted in a climate chamber set to 20 °C and at 95% humidity before plunge frozen in liquid ethane set at −183 °C using a Leica EM GP system (Leica Microsystems) ...
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Computational toolbox for ultrastructural quantitative analysis of filament networks in cryo-ET databioRxiv - Cell Biology 2021Quote: B16-F1 cells were cultured as described above and seeded onto 200 mesh gold holey carbon grids (R2/2-2C; Quantifoil Micro Tools). Prior to cell seeding ...
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bioRxiv - Cell Biology 2021Quote: ... Glow-discharged holey Quantifoil grids with an additional layer of continuous carbon (R2/1 + 2 nm C, Cu 200 mesh grid, Quantifoil Micro Tools) were used ...
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Mycobacteria form viable cell wall-deficient cells that are undetectable by conventional diagnosticsbioRxiv - Microbiology 2022Quote: ... of which 3-4 µL was applied on glow-discharged 200 mesh copper grids with an extra thick R2/2 carbon film (Quantifoil Micro Tools). Vitrification was performed using an automated Leica EM GP plunge-freezer ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μl of the resulting mixture was spotted onto glow-discharged copper EM grids (Quantifoil 300 Au mesh, R2/2 carbon film (Quantifoil Micro Tools). The grids were then blotted for 2 or 4 seconds (blot force −10 ...
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bioRxiv - Cell Biology 2022Quote: ... 3 µl of the microtubule sample was deposited at the surface of a glow-discharged holey carbon grid (Quantifoil R2/2, Cu200) in the temperature (35 °C ...