Labshake search
Citations for Quantifoil Micro Tools :
1 - 50 of 292 citations for 6 FLUORO 1 HEXANOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Aliquots of 4 μl of protein samples (0.3 mg ml-1 or ∼6 μM) were applied to glow-discharged R1.2/1.3 200 Cu mesh grids (Quantifoil). After 30 s ...
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bioRxiv - Neuroscience 2023Quote: ... Double application of 3 µL protein (∼0.6 mg/mL) SYM2081 complex was carried out on glow-discharged gold grids (R 0.6/1, 300 mesh, Quantifoil). The grids were blotted for 4.5 s and 3.5 s ...
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bioRxiv - Biophysics 2021Quote: To provide extra support for cell growth 5-6 nm of carbon was evaporated onto Quantifoil Au 200 R2/1 or R2/2 grids (Quantifoil Micro Tools GmbH). Grids were then glow discharged and incubated in supplemented RPMI-1640 overnight in the cell incubator ...
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bioRxiv - Biophysics 2020Quote: ... This sample was used for EM dataset 6: the purified sample was applied to Cu 300 R1.2/1.3 holey carbon grids (Quantifoil) coated with a ∼7 nm homemade carbon film ...
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bioRxiv - Biophysics 2020Quote: ... 3.5 µl of purified channel proteins at ∼6 mg/ml were pipetted onto glow-discharged copper Quantifoil R2/2 holey carbon grids (Quantifoil). Grids were blotted for 2 s at ∼100% humidity and flash frozen in liquid ethane ...
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bioRxiv - Cell Biology 2021Quote: ... Aggregation was stimulated with TRAP-6 (25μM) in a 96-well plate (15 minutes, 750rpm, 37°C; BioShake IQ, Quantifoil Instruments) and fixed with 1% formalin (Sigma) ...
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bioRxiv - Biophysics 2022Quote: 3.5 μl of protein samples at 4-6 mg/ml were applied to glow-discharged QF R1.2/1.3 300 mesh gold grids (Quantifoil, Großlöbichau, Germany). Grids were blotted for 3 s at 4°C and 100% humidity and plunge-frozen into liquid ethane using FEI Mark IV Vitrobot (FEI ...
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bioRxiv - Biophysics 2019Quote: 3 µL of purified aldolase (1.6 mg/mL) or apoferritin (5 mg/mL) was dispensed on UltrAuFoil R1.2/1.3 300-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly plasma cleaned for six seconds at 15Watts (75% nitrogen/25% oxygen atmosphere ...
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bioRxiv - Cell Biology 2022Quote: The cryo-EM grid was prepared by applying 3 μl of the protein samples (4 – 6 mg/ml) to glow-discharged Quantifoil R1.2/1.3 300-mesh gold holey carbon grids (Quantifoil, Micro Tools GmbH, Germany). Grids were blotted for 4.0 seconds under 100% humidity at 4°C before being plunged into the liquid ethane using a Mark IV Vitrobot (FEI) ...
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bioRxiv - Biophysics 2021Quote: ... 3 μl of purified ghrelin-bound complex at 13 mg/ml and GHRP-6-bound ghrelin receptor complex at 8 mg/ml were applied individually onto a glow-discharged holey carbon grid (Quantifoil, Au300 R1.2/1.3) in a Vitrobot chamber (FEI Vitrobot Mark IV) ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µl of MHAS2168-Mb_H2 complex (9.4 mg/ml and 6 mg/ml) were applied to glow-discharged (45 s) holey carbon grids (Quantifoil R1.2/1.3 Au 200 mesh) and a Grid Plunger GP2 (Leica ...
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bioRxiv - Biophysics 2019Quote: An aliquot of 4 μl of 6 μM Ac-E46K α-syn fibril solution was applied to a glow-discharged holey carbon grid (Quantifoil R1.2/1.3, 300 mesh), blotted for 6.5 s ...
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bioRxiv - Biophysics 2020Quote: ... 2 μL of a 1 in 6 dilution of the batch crystallisation was applied to the carbon side of glow discharged R2/2 Quantifoil™ grids (Quantifoil Micro Tools, Jena, Germany). Excess liquid was removed by blotting for 4-6 s with a Vitrobot (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: Aliquots of 3.5 μL SbtA-SbtB (ΔT-loop) samples (∼6 mg/mL) were applied to the glow-discharged grids (Quantifoil holey carbon Cu R1.2/1.3 grid). Grids were blotted for 4.5 sec and plunge-frozen in liquid ethane vitrified by liquid nitrogen using Vitrobot Mark IV (FEI Company ...
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bioRxiv - Biochemistry 2024Quote: ... or calpain-3-titin/I81-I83 (2mg/mL) from Superose 6 increase 10/300GL column chromatography was loaded onto glow-discharged holey carbon grids (Quantifoil 1.2/1.3 + 2 nm Carbon). The grids were blotted with filter paper for 2.5 secs at 22 °C and then immediately plunged into liquid ethane cooled by liquid nitrogen using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... Four microliters of purified Cx36 proteins (4-6 mg/ml) were applied onto a negatively glow-discharged holey carbon grid (Quantifoil R1.2/1.3 Cu 300 mesh). For samples involving Cx36-WT ...
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bioRxiv - Bioengineering 2022Quote: ... holey carbon grids (Quantifoil R2/1 grid: Cu 300mesh, M2951C-1-300) were hydrophilized by a JEC-3000FC Auto Fine Coater (JEOL ...
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bioRxiv - Cell Biology 2019Quote: ... R 2/1 or R 3.5/1 on 300 mesh Cu/Rh grids (QUANTIFOIL) were glow discharged for 45 s at 25 mA ...
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bioRxiv - Molecular Biology 2021Quote: ... Cu R2/1 grids (Quantifoil) were plasma cleaned using a NanoClean plasma cleaner (Fischione Instruments ...
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bioRxiv - Biophysics 2019Quote: ... Quantifoil 200 mesh holey carbon R4/1 or R2/1 copper grids (Quantifoil Micro Tools) were glow discharged for 60 s at 0.2 mbar with 20 mA using a PELCO easiGlow glow discharge system (Ted Pella) ...
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bioRxiv - Biochemistry 2022Quote: ... R3.5/1 Cu 200 grids (Quantifoil) were glow-discharged on a glass clover slide for 60 seconds at 25 mA and plunging into liquid ethane was performed using an EM GP (Leica Microsystems) ...
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bioRxiv - Neuroscience 2020Quote: Holey carbon grids (Quantifoil R2/1) covered with either 2 nm amorphous carbon (Quantifoil ...
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bioRxiv - Cell Biology 2023Quote: Quantifoil R3.5/1 Au200 grids (Quantifoil) were glow discharged for 30 s in an Auto306 Edwards Turbo Coater at power 6 (20 mA ...
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bioRxiv - Biochemistry 2021Quote: UltrAuFoil 0.6/1 300 mesh grids (Quantifoil) were glow discharged in residual air at 25 mA for 3 min using a Pelco EasiGLOWTM ...
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bioRxiv - Biochemistry 2022Quote: UltrAuFoil® gold grids (0.6/1, Quantifoil) (Russo and Passmore ...
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bioRxiv - Biochemistry 2023Quote: ... UltrAuFoil 0.6/1 300-mesh grids (Quantifoil) were glow discharged in residual air at 20LmA for 60 s each side using a Pelco EasiGLOW ...
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bioRxiv - Biochemistry 2021Quote: ... were applied to glow discharged (2.2×10-1 mbar for 20 sec) Quantifoil holey carbon grids (R2/1, 200 mesh, Quantifoil). The grids were plunge vitrified into a liquid ethane/propane mix using a Vitrobot Mark IV at 4 °C and 95% humidity ...
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bioRxiv - Biochemistry 2020Quote: ... Quantifoil 300 mesh gold grids (either 0.6/1 carbon film, 0.6/1 or 1.2/1.3 UltrAUfoil, Quantifoil Micro Tools GMBH) were glow discharged for 30 seconds prior to use ...
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bioRxiv - Biochemistry 2019Quote: ... 4 μl of these samples were applied to glow discharged (2.2×10-1 mbar for 2×20sec) Quantifoil holey carbon grids (R2/1, 200 mesh, Quantifoil). The grids were plunge vitrified into a liquid ethane/propane mix using a Vitrobot Mark IV at 4 °C and 95% humidity ...
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bioRxiv - Biophysics 2023Quote: ... using FluoSpheres (diameter of 1 μm) on gold Quantifoil grids (200 mesh, R 2/1, holey SiO2 film; Quantifoil Micro Tools GmbH ...
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bioRxiv - Plant Biology 2022Quote: 3 μL of purified PSI complex at 1 mg Chl mL−1 were applied to glow-discharged (GloQube Quorum, 40 sec, 20mA) holey carbon grids (Quantifoil 300 Au R1.2/R1.3 ...
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bioRxiv - Biophysics 2020Quote: Holey carbon grids (Quantifoil R2/1, 200 mesh) were plasma treated using a Solarus plasma cleaner (Gatan) ...
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bioRxiv - Plant Biology 2021Quote: Holey carbon grids (Quantifoil R2/1, 200 mesh) were plasma cleaned using a Solarus plasma cleaner (Gatan) ...
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bioRxiv - Molecular Biology 2020Quote: ... transferred onto allgold UltrAuFoil R0.6/1 grids (QuantiFoil), and partially hydrogenated following a previously described procedure [17] ...
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bioRxiv - Molecular Biology 2022Quote: ... The no carbon sample (Quantifoil Cu200 R2/1) was blotted for 1.5 s from the back side right after sample application and immediately plunge frozen in liquid ethane ...
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bioRxiv - Molecular Biology 2022Quote: R3.5/1 Holey carbon copper grids (QUANTIFOIL, Germany) were glow-discharged by UV light ...
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bioRxiv - Cell Biology 2023Quote: ... 200 mesh R2/1 Au EM grids (Quantifoil) were coated with laminin (1:10 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... Cu supported 3.5/1 Holey Carbon grids (Quantifoil) were glow discharged on both sides for six seconds each using atmospheric air in order to render them more hydrophilic ...
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bioRxiv - Biophysics 2022Quote: A 3 µl aliquot of freshly concentrated or thawed protein at ∼1 mg/ml was deposited on an UltraAuFoil 300 mesh R 0.6/1 grid (Quantifoil Inc., UK) at 4 °C and 100% humidity in a Vitrobot Mark IV robot (Thermo Fisher) ...
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bioRxiv - Pathology 2023Quote: ... bombycis spores (1×108 spores/mL) were placed on grids (Cu R2/1, 200 mesh) (Quantifoil Micro Tools GmbH, Jena, Germany) for 5 min and germinated in 0.1 M KOH for 15 min at 30°C ...
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bioRxiv - Microbiology 2023Quote: ... sample were placed onto glow-discharged Quantifoil R2/1 Cu300 holey carbon grids (1 µm spacing, 2 µm holes, copper mesh) (Quantifoil, Germany), blotted for 3.5 s before flash-freezing in liquid ethane using a Vitrobot mark IV (FEI ...
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bioRxiv - Biochemistry 2023Quote: 4 µL of protein solution (total protein concentration = 1 mg/mL) were applied to freshly glow-discharged QUANTIFOIL® R2/1 300 mesh grids (Quantifoil Micro Tools GmbH ...
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bioRxiv - Cell Biology 2022Quote: 2 x 104 TauRD-Y* cells or 1 x 105 neurons were seeded on EM grids (R2/1, Au 200 mesh grid, Quantifoil Micro Tools) in a 35 mm dish or 24-well plate and cultured for 24 h or transduced with lentivirus and treated with aggregate-containing cell lysate as described earlier in section ‘Primary neuronal cultures’ ...
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bioRxiv - Microbiology 2020Quote: ... 3.5 μl of the freshly prepared mixture of the soluble S trimer and monomeric ACE2 (1:3 molar ratio) at ~1 mg/ml was applied to a 1.2/1.3 Quantifoil grid (Quantifoil Micro Tools GmbH, Germany), which had been glow discharged with a PELCO easiGlow™ Glow Discharge Cleaning system (Ted Pella ...
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bioRxiv - Microbiology 2021Quote: ... holey carbon grids (Quantifoil R2/1 200 mesh copper) for 1 min ...
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bioRxiv - Biochemistry 2022Quote: UltrAuFoil gold grids (0.6/1, Quantifoil Micro Tools GmbH)56 were prepared as described previously9 ...
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bioRxiv - Biophysics 2019Quote: ... perforated carbon copper grid (Quantifoil R2/1 200 mesh). The nuclei were opened manually and the nuclear envelopes spread over the grid ...
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bioRxiv - Cell Biology 2021Quote: Holey carbon grids (Quantifoil R2/1, 200 mesh, gold) were washed in acetone while agitating on a rocker for 15 to 30 min to remove potential residual plastic backing ...
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bioRxiv - Biochemistry 2023Quote: ... holey carbon grids (Quantifoil, 300 mesh Au R0.6/1) glow-discharged for 50 seconds were used for EM grids preparation ...
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bioRxiv - Cell Biology 2023Quote: ... Cryo-EM grid (Quantifoil R3.5/1, Au 200 mesh) was glow discharged using Gatan Solarus for 60 s ...