Labshake search
Citations for Quantifoil Micro Tools :
351 - 400 of 435 citations for 6 Chloro 2 2 dimethyl 4 chromanone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... with holey R 1/4 carbon film (Quantifoil Micro Tools GmbH) were glow-discharged for 30 seconds at 5 mA using an EasiGlow system (Pelco ...
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bioRxiv - Biophysics 2020Quote: ... This sample was used for EM dataset 6: the purified sample was applied to Cu 300 R1.2/1.3 holey carbon grids (Quantifoil) coated with a ∼7 nm homemade carbon film ...
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bioRxiv - Biophysics 2023Quote: ... Aliquots of 4 μl of protein samples (0.3 mg ml-1 or ∼6 μM) were applied to glow-discharged R1.2/1.3 200 Cu mesh grids (Quantifoil). After 30 s ...
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bioRxiv - Neuroscience 2023Quote: ... Double application of 3 µL protein (∼0.6 mg/mL) SYM2081 complex was carried out on glow-discharged gold grids (R 0.6/1, 300 mesh, Quantifoil). The grids were blotted for 4.5 s and 3.5 s ...
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bioRxiv - Microbiology 2023Quote: ... Gold grids with SiO2 film (Quantifoil, Au 200 mesh, R 1/4) were glow- discharged and coated with 20 µg/ml fibronectin solution for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... Aggregation was stimulated with TRAP-6 (25μM) in a 96-well plate (15 minutes, 750rpm, 37°C; BioShake IQ, Quantifoil Instruments) and fixed with 1% formalin (Sigma) ...
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bioRxiv - Biophysics 2021Quote: ... with 12-nm-thick holey R1/4 and R1.2/20 SiO2 film (Quantifoil) were used for the experiments ...
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bioRxiv - Cell Biology 2021Quote: ... Gold Quantifoil grids (R1/4, Au 200 mesh grid, SiO2, Quantifoil Micro Tools) were glow-discharged and UV irradiated for 30 min for sterilization before being immersed in cell culture medium in 35-mm IBIDI μ-Dish ...
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bioRxiv - Synthetic Biology 2023Quote: 4 µl of sample was adsorbed onto holey carbon-coated grid (Quantifoil, Germany) that was glow-discharged ...
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bioRxiv - Biophysics 2019Quote: 3 µL of purified aldolase (1.6 mg/mL) or apoferritin (5 mg/mL) was dispensed on UltrAuFoil R1.2/1.3 300-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly plasma cleaned for six seconds at 15Watts (75% nitrogen/25% oxygen atmosphere ...
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bioRxiv - Biophysics 2020Quote: ... 4 µl sample was applied to each freshly glow-discharged grid (Quantifoil, R1.2/1.3). The grids were then plunge-frozen in liquid ethane ...
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bioRxiv - Microbiology 2021Quote: ... 4-7 µl of cells were deposited on R2/1 Cu 200 grids (Quantifoil) that had been glow-discharged for 1 min at 0.19 mbar and 20 mA in a PELCO easiGlow device shortly before use ...
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bioRxiv - Biophysics 2021Quote: ... 4 μl of cell suspension was applied to holey carbon R2/1 Cu grids (Quantifoil) and plunge-frozen on a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... 4 μL of sample was applied to glow-discharged EM grids (R2/1 copper, Quantifoil), blotted twice from the back for 3.5 s and vitrified in liquid ethane–propane using a Vitrobot Mark IV (ThermoFisher) ...
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bioRxiv - Biochemistry 2023Quote: ... 4 μl of sample was applied to glow discharged Quantifoil 300 mesh 1.2/1.3 (Quantifoil) grids at 4 °C and 90-100% humidity ...
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bioRxiv - Biophysics 2024Quote: ... 4 µl of this sample was then applied to plasma-cleaned holey carbon grids (Quantifoil) and plunge frozen with an FEI vitrobot mark IV at 4°C and 100% humidity.
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bioRxiv - Cell Biology 2024Quote: Quantifoil R2/1 or SiO2 R1/4 gold grids (both Quantifoil Mirco Tools GmbH, Germany) were briefly washed in chloroform and placed on drops of poly-D-lysine (100 µg/ml) ...
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bioRxiv - Biophysics 2021Quote: ... 3 μl of purified ghrelin-bound complex at 13 mg/ml and GHRP-6-bound ghrelin receptor complex at 8 mg/ml were applied individually onto a glow-discharged holey carbon grid (Quantifoil, Au300 R1.2/1.3) in a Vitrobot chamber (FEI Vitrobot Mark IV) ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µl of MHAS2168-Mb_H2 complex (9.4 mg/ml and 6 mg/ml) were applied to glow-discharged (45 s) holey carbon grids (Quantifoil R1.2/1.3 Au 200 mesh) and a Grid Plunger GP2 (Leica ...
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bioRxiv - Biochemistry 2020Quote: ... 4 µL of sample was applied to Quantifoil Cu R 1.2/1.3 grids (Quantifoil Microtools GmbH) which has undergone a plasma discharge and were vitrified in liquid ethane using a Vitrobot MkIV plunge freezer (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... Aliquots of 4 µl were applied to glow-discharged 300 mesh R1.2/1.3 Au grids (Quantifoil) using a Vitrobot Mark VI (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... 4 μL of Kapβ2•HNRNPH2 was applied to a 300 mesh copper grid (Quantifoil R1.2/1.3) that was glow-discharged using a PELCO easiGlow glow discharge apparatus at 30 mA/30 s on top of a metal grid holder (Ted Pella) ...
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bioRxiv - Cell Biology 2023Quote: ... Each glow-discharged Quantifoil R2/4 200 mesh copper grid (Quantifoil Micro Tools GmbH, Jena, Germany) was then deposited with 4 µL sample and plunge-frozen in liquid ethane using a Vitrobot Mark IV (TFS ...
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bioRxiv - Microbiology 2023Quote: Aliquots of 3.5 μL SbtA-SbtB (ΔT-loop) samples (∼6 mg/mL) were applied to the glow-discharged grids (Quantifoil holey carbon Cu R1.2/1.3 grid). Grids were blotted for 4.5 sec and plunge-frozen in liquid ethane vitrified by liquid nitrogen using Vitrobot Mark IV (FEI Company ...
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bioRxiv - Biophysics 2022Quote: A volume of 4-7 µl of cells were deposited on R2/1 Cu 200 grids (Quantifoil) that had been glow-discharged for 1 min at 0.19 mbar and 20 mA in a PELCO easiGlow device shortly before use ...
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bioRxiv - Biophysics 2019Quote: ... Perforated carbon film-covered microscopical 200 mesh grids (R1/4 batch of Quantifoil, MicroTools GmbH, Jena, Germany) were cleaned with chloroform and hydrophilised by 60 s glow discharging at 8 W in a BAL-TEC MED 020 device (Leica Microsystems ...
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bioRxiv - Biophysics 2020Quote: ... 0.2 mM ATP) and 4 μl were applied to glow-discharged 1.2/1.3 holey gold grids (Quantifoil) before plunge freezing in liquid ethane using a Vitrobot (FEI/Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... 4 µl of the sample were applied onto 300 mesh R1.2/1.3 UltrAuFoil Holey Gold Films (Quantifoil), that had been previously plasma treated for 30 seconds using a 15 mA current operating under atmospheric gases using a glow discharger (Electron Microscopy Sciences) ...
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bioRxiv - Biochemistry 2021Quote: 4 µl of 20-100 nM RZZSF filaments were deposited on freshly glow-discharged Formvar/Carbon (Quantifoil) film supported copper grid Cu400 (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 μl of the sample were applied onto 300 mesh R1.2/1.3 UltrAuFoil Holey Gold Films (Quantifoil) that were plasma cleaned prior to sample application for 7 seconds using a Solarus plasma cleaner (Gatan ...
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bioRxiv - Biophysics 2022Quote: ... 4 μL of the TnsBCTD-TnsC-TniQ sample was loaded on UltrAuFoil R1.2/1.3 gold grids (Quantifoil) and vitrified using the Mark IV Vitrobot (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: Primary cultured neurons were seeded onto holey gold EM grids (Quantifoil R 1/4 gold 200 mesh). The fiducial marker solution was prepared as for neocortical synaptosomes ...
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bioRxiv - Biophysics 2023Quote: 4 μL purified protein was placed on the holy-carbon grids (Quantifoil Au R1.2/1.3, 300 mesh), which were glow-discharged for 30 s ...
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bioRxiv - Biophysics 2023Quote: ... 4 µL fresh sample was applied on glow-discharged Cu holey carbon grids (Quantifoil R 1.2/1.3) and incubated for 1 min at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Quantifoil Holey SiO2 R 1/4 Au 200 mesh EM grids (Quantifoil Micro Tools #N1-s13nAi20-01) were glow discharged on both sides in a Pelco easyglow for 45 s at 0.37 mBar and 15 mA ...
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bioRxiv - Biochemistry 2021Quote: ... 3 microlitres of undiluted eluate and of eluate which was diluted 1:2 or 1:4 in buffer A200 were blotted onto holey carbon R 1.2/1.3 copper 300 mesh grids (Quantifoil) and vitrified in liquid ethane using a Vitrobot MarkIV device (Thermofisher) ...
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bioRxiv - Biophysics 2019Quote: Samples for cryo-EM were prepared by applying 4 µl of purified TRPA1 to 1.2/1.3 holey carbon grids (Quantifoil) and blotting for 8-12 sec ...
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bioRxiv - Biophysics 2021Quote: ... 4 µL of aldolase sample were applied on UltrAuFoil R1.2/1.3 300-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly plasma cleaned for 6 seconds at 15 Watts (75% nitrogen/25% oxygen atmosphere ...
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bioRxiv - Biophysics 2021Quote: ... 4 µL of apoferritin sample were applied on UltrAuFoil R1.2/1.3 300-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly plasma cleaned for 7 seconds at 15 Watts (75% nitrogen/25% oxygen atmosphere ...
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bioRxiv - Biophysics 2021Quote: The LlCsm RNP and RNP-target RNA samples at approximately 0.5 mg/ml were separately applied in 4 μL volume onto glow-discharged UltrAuFoil 300 mesh R1.2/1.3 grids (Quantifoil), blotted for 3 s at 88% humidity and flash-frozen in liquid ethane using FEI Vitrobot Mark IV ...
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bioRxiv - Molecular Biology 2022Quote: ... for 15 min before 4 μL of sample was applied to a graphene oxide coated 1.2/1.3 UltrAuFoil grids (Quantifoil) at 4°C under 100% humidity86 ...
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bioRxiv - Microbiology 2022Quote: ... Aliquots of 4 μL samples were applied to glow-discharged holey carbon girds (Cu, R1.2/1.3, 300 mesh, Quantifoil). The grids were blotted with force 2 for 3 seconds and plunged into liquid ethane using a Vitrobot (FEI Thermo Fisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... The peak fraction was then diluted with buffer N to 0.8 mg ml-1 and cryoEM grids prepared by applying 4 µl of the sample to glow-discharged Cu R1.2/1.3 300-mesh holey carbon grids (Quantifoil). Grids were prepared blotted at 8 °C and 90% humidity and plunged into liquid ethane using a Leica EM GP2.
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bioRxiv - Biochemistry 2024Quote: ... 4 uL of the concentrated sample was applied to freshly glow-discharged R2/1 holey carbon grids (Quantifoil), blotted with blot force of 5 for 5 s using a Vitrobot Mark IV (Thermo Fisher ...
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bioRxiv - Biochemistry 2020Quote: ... KBP was diluted to 0.3 mg/ml in KBP dilution buffer and 4 μl were applied to glow-discharged 1.2/1.3 AuFoil gold grids (Quantifoil®). For the third approach ...
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bioRxiv - Molecular Biology 2019Quote: The concentration of the core BBSome as derived from chemical crosslinking was adjusted to 0.05-0.08 mg/ml and 4 μl of sample was immediately applied to a glow-discharged UltrAuFoil® R1.2/1.3 holey gold grid (Quantifoil). After two minutes incubation at 13 °C and 100% relative humidity (RH) ...
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bioRxiv - Molecular Biology 2021Quote: Purified mtLSU or 55S mitoribosome samples (4 μL) were applied to freshly glow discharged R 3.5/1 holey carbon grids (Quantifoil) that were precoated with a 2-3 nm carbon layer using a Leica EM ACE600 coater ...
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bioRxiv - Microbiology 2022Quote: ... A 4□μl volume of SsoCsm complex was applied to holey carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh) covered by a graphene oxide layer (27) ...
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bioRxiv - Biophysics 2021Quote: ... Log phase cultures were centrifuged at 2000 g for 2 minutes to concentrate the cells 10 times and 4 μl was directly applied to a glow-discharged holey carbon R2/1 Cu 200 mesh grid (Quantifoil). Plunge-freezing was performed using a VitroBot Mark 4 (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... then 4 µl of the sample was applied to glow-discharged holey carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh). The grid was blotted for 4s and then plunged into liquid ethane using a Vitrobot Mark IV (FEI ...