Labshake search
Citations for Quantifoil Micro Tools :
351 - 400 of 615 citations for 6 Bromo 1 2 cyanoethyl 2 4 diethylamino phenyl benz cd indolium chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2021Quote: ... Log phase cultures were centrifuged at 2000 g for 2 minutes to concentrate the cells 10 times and 4 μl was directly applied to a glow-discharged holey carbon R2/1 Cu 200 mesh grid (Quantifoil). Plunge-freezing was performed using a VitroBot Mark 4 (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2023Quote: ... trehalose solution to mitigate crystalline ice formation and 4 μL of this mixture immediately applied to a R2/1 Cu 200 grid (Quantifoil), which had been glow-discharged for 1 min at 0.19 mbar and 20 mA in a PELCO easiGlow device shortly before use ...
-
bioRxiv - Biophysics 2023Quote: ... 4 µL fresh sample (0.48 mg/mL) was applied on glow-discharged Au holey carbon grids (Quantifoil R 0.6/1) and the following operation were the same as describe above for preparing the sample of gMCM8/9 complex.
-
bioRxiv - Biophysics 2023Quote: ... 200 mesh R1/4 or R2/4 Quantifoil holy carbon gold grids (Quantifoil®, Grossloebichau/Jena Germany). were glow discharged in Pelco easiGlow (Ted Pella ...
-
bioRxiv - Biophysics 2019Quote: ... Approximately 5 µL of sample (diluted if necessary) was placed on a copper grid (Quantifoil® S7/2 Cu 400 mesh, holey carbon films, Quantifoil Micro Tools GmbH, Jena, Germany) and any excess liquid was absorbed by a filter paper ...
-
bioRxiv - Biochemistry 2022Quote: The samples were frozen on copper or gold grids with holey carbon film coated with a 2 nm continuous carbon film (Quantifoil R1.2/1.3 ultrathin carbon, 300 mesh) freshly glow discharged for 20 s at 15 mA (PELCO easiGlow) ...
-
bioRxiv - Neuroscience 2023Quote: ... 3.5 μL of CopiaGag capsid protein sample was applied to a 300-mesh copper grid with continuous 2 nm carbon film (Electron Microscopy Sciences, QUANTIFOIL® C2-C15nCu30-50) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
-
bioRxiv - Molecular Biology 2021Quote: ... cells in suspension (4 μl of ~1,000 cells per μl) were blotted onto R2/1 carbon-coated 200-mesh copper grids (Quantifoil Micro Tools) and plunge frozen in a liquid ethane/propane mixture ...
-
bioRxiv - Biochemistry 2023Quote: ... A 3 µL drop was applied to a glow-discharged (PELCO easiGlow, 15 mA, 2 min) holey carbon grid (Quantifoil R1.2/1.3 on gold 200 mesh support), blotted for 3-4 seconds with Whatman Grade 595 filter paper (GE Healthcare) ...
-
bioRxiv - Molecular Biology 2024Quote: ... about 0.9 mg/ml for DSS1–INTAC–PEC complex) was applied onto a glow-discharged holey carbon grid (Quantifoil Au, R1.2/1.3 or R2/2, 300 mesh). After blotting for 3 to 6 s ...
-
bioRxiv - Cell Biology 2024Quote: ... the sample was gently resuspended and 4 µl was transferred to a glow-discharged holey carbon R2/1 copper grid (Quantifoil Micro Tools) suspended in the chamber of a Vitrobot (Thermo Fisher Scientific) ...
-
bioRxiv - Biochemistry 2019Quote: 2.5 µl of freshly purified protein at a concentration of 3.3 mg/ml when solubilized in DDMand at about 2 mg/ml when reconstituted in nanodiscs were applied on holey-carbon cryo-EM grids (Quantifoil Au R1.2/1.3, 200, 300 and 400 mesh), which were prior glow-discharged at 5 mA for 30 s ...
-
bioRxiv - Biochemistry 2022Quote: Freshly prepared ribosome–Sec61–KZR-8445 complex at 300–500 nM concentration was applied on glow discharged holey-carbon grids (Quantifoil, R1.2/1.3 with a 2-nm carbon film) and flash frozen with an automated plunge freezer (Leica microsystems) ...
-
bioRxiv - Cell Biology 2020Quote: ... or holey SiO2 R1/4 TEM grids (Quantifoil) to allow for more robust correlation and cells were plunge frozen on a Vitrobot Mark IV (FEI ...
-
bioRxiv - Cell Biology 2022Quote: ... Grids (200 Mesh Cu SiO2 R1/4, Quantifoil) were plasma cleaned for 30 s before plunging ...
-
bioRxiv - Molecular Biology 2023Quote: ... a 4 µl sample of purified complex was applied to glow-discharged R2/1 300 mesh holey carbon copper grids (Quantifoil Micro Tools GmbH), using a conc ...
-
bioRxiv - Cell Biology 2023Quote: 4 ul of Nannochloropsis suspensions (4 x 107 cells mL−1) were pipetted onto a 300 mesh gold Quantifoil grid with 2um holes (Quantifoil Micro Tools, GmbH) and manually blotted from the opposite side in a Vitrobot Mark IV (ThermoFisher Scientific ...
-
bioRxiv - Biochemistry 2022Quote: 4 µl aliquots of purified samples at A280 = 1 were applied onto glow-discharged holey carbon-coated grids (Quantifoil 300 mesh, Au R1.2/1.3), then adsorbed for 10 s ...
-
bioRxiv - Biophysics 2021Quote: Gold EM grids with R1/4 holey SiO2 film (Quantifoil) were glow-discharged in a EasiGlow (Pelco ...
-
bioRxiv - Cell Biology 2019Quote: ... R1/4 or R1/20 (Quantifoil Micro Tools, Jena, Germany) were employed in this study ...
-
bioRxiv - Biophysics 2023Quote: 200 mesh Au grids with R1/4 SiO2 foil (Quantifoil) were glow-discharged for 90 sec at 0.38 mBar and 15 mA using a Pelco easiGlow device ...
-
bioRxiv - Neuroscience 2022Quote: ... Gold Quantifoil grids (R1/4, Au 200 mesh, Quantifoil Micro Tools) were additionally coated with a 20-25 nm carbon layer (MED 020 ...
-
bioRxiv - Biophysics 2020Quote: ... This sample was used for EM dataset 6: the purified sample was applied to Cu 300 R1.2/1.3 holey carbon grids (Quantifoil) coated with a ∼7 nm homemade carbon film ...
-
bioRxiv - Cell Biology 2021Quote: ... Aggregation was stimulated with TRAP-6 (25μM) in a 96-well plate (15 minutes, 750rpm, 37°C; BioShake IQ, Quantifoil Instruments) and fixed with 1% formalin (Sigma) ...
-
bioRxiv - Biophysics 2021Quote: ... with 12-nm-thick holey R1/4 and R1.2/20 SiO2 film (Quantifoil) were used for the experiments ...
-
bioRxiv - Cell Biology 2021Quote: ... Gold Quantifoil grids (R1/4, Au 200 mesh grid, SiO2, Quantifoil Micro Tools) were glow-discharged and UV irradiated for 30 min for sterilization before being immersed in cell culture medium in 35-mm IBIDI μ-Dish ...
-
bioRxiv - Synthetic Biology 2023Quote: 4 µl of sample was adsorbed onto holey carbon-coated grid (Quantifoil, Germany) that was glow-discharged ...
-
bioRxiv - Biophysics 2019Quote: 3 µL of purified aldolase (1.6 mg/mL) or apoferritin (5 mg/mL) was dispensed on UltrAuFoil R1.2/1.3 300-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly plasma cleaned for six seconds at 15Watts (75% nitrogen/25% oxygen atmosphere ...
-
bioRxiv - Biophysics 2020Quote: ... 4 µl sample was applied to each freshly glow-discharged grid (Quantifoil, R1.2/1.3). The grids were then plunge-frozen in liquid ethane ...
-
bioRxiv - Biochemistry 2023Quote: ... 4 μl of sample was applied to glow discharged Quantifoil 300 mesh 1.2/1.3 (Quantifoil) grids at 4 °C and 90-100% humidity ...
-
bioRxiv - Biophysics 2024Quote: ... 4 µl of this sample was then applied to plasma-cleaned holey carbon grids (Quantifoil) and plunge frozen with an FEI vitrobot mark IV at 4°C and 100% humidity.
-
bioRxiv - Biophysics 2021Quote: ... 3 μl of purified ghrelin-bound complex at 13 mg/ml and GHRP-6-bound ghrelin receptor complex at 8 mg/ml were applied individually onto a glow-discharged holey carbon grid (Quantifoil, Au300 R1.2/1.3) in a Vitrobot chamber (FEI Vitrobot Mark IV) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 4 µl of MHAS2168-Mb_H2 complex (9.4 mg/ml and 6 mg/ml) were applied to glow-discharged (45 s) holey carbon grids (Quantifoil R1.2/1.3 Au 200 mesh) and a Grid Plunger GP2 (Leica ...
-
bioRxiv - Biochemistry 2020Quote: ... 4 µL of sample was applied to Quantifoil Cu R 1.2/1.3 grids (Quantifoil Microtools GmbH) which has undergone a plasma discharge and were vitrified in liquid ethane using a Vitrobot MkIV plunge freezer (Thermo Fisher Scientific) ...
-
bioRxiv - Biophysics 2021Quote: ... Aliquots of 4 µl were applied to glow-discharged 300 mesh R1.2/1.3 Au grids (Quantifoil) using a Vitrobot Mark VI (Thermo Fisher Scientific ...
-
bioRxiv - Biophysics 2023Quote: ... 4 μL of Kapβ2•HNRNPH2 was applied to a 300 mesh copper grid (Quantifoil R1.2/1.3) that was glow-discharged using a PELCO easiGlow glow discharge apparatus at 30 mA/30 s on top of a metal grid holder (Ted Pella) ...
-
bioRxiv - Cell Biology 2023Quote: ... Each glow-discharged Quantifoil R2/4 200 mesh copper grid (Quantifoil Micro Tools GmbH, Jena, Germany) was then deposited with 4 µL sample and plunge-frozen in liquid ethane using a Vitrobot Mark IV (TFS ...
-
bioRxiv - Microbiology 2023Quote: Aliquots of 3.5 μL SbtA-SbtB (ΔT-loop) samples (∼6 mg/mL) were applied to the glow-discharged grids (Quantifoil holey carbon Cu R1.2/1.3 grid). Grids were blotted for 4.5 sec and plunge-frozen in liquid ethane vitrified by liquid nitrogen using Vitrobot Mark IV (FEI Company ...
-
bioRxiv - Biophysics 2019Quote: ... Perforated carbon film-covered microscopical 200 mesh grids (R1/4 batch of Quantifoil, MicroTools GmbH, Jena, Germany) were cleaned with chloroform and hydrophilised by 60 s glow discharging at 8 W in a BAL-TEC MED 020 device (Leica Microsystems ...
-
bioRxiv - Biophysics 2020Quote: ... 0.2 mM ATP) and 4 μl were applied to glow-discharged 1.2/1.3 holey gold grids (Quantifoil) before plunge freezing in liquid ethane using a Vitrobot (FEI/Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2019Quote: ... 4 µl of the sample were applied onto 300 mesh R1.2/1.3 UltrAuFoil Holey Gold Films (Quantifoil), that had been previously plasma treated for 30 seconds using a 15 mA current operating under atmospheric gases using a glow discharger (Electron Microscopy Sciences) ...
-
bioRxiv - Biochemistry 2021Quote: 4 µl of 20-100 nM RZZSF filaments were deposited on freshly glow-discharged Formvar/Carbon (Quantifoil) film supported copper grid Cu400 (Sigma Aldrich ...
-
bioRxiv - Molecular Biology 2020Quote: ... 4 μl of the sample were applied onto 300 mesh R1.2/1.3 UltrAuFoil Holey Gold Films (Quantifoil) that were plasma cleaned prior to sample application for 7 seconds using a Solarus plasma cleaner (Gatan ...
-
bioRxiv - Biophysics 2022Quote: ... 4 μL of the TnsBCTD-TnsC-TniQ sample was loaded on UltrAuFoil R1.2/1.3 gold grids (Quantifoil) and vitrified using the Mark IV Vitrobot (ThermoFisher ...
-
bioRxiv - Biophysics 2023Quote: 4 μL purified protein was placed on the holy-carbon grids (Quantifoil Au R1.2/1.3, 300 mesh), which were glow-discharged for 30 s ...
-
bioRxiv - Biophysics 2023Quote: ... 4 µL fresh sample was applied on glow-discharged Cu holey carbon grids (Quantifoil R 1.2/1.3) and incubated for 1 min at room temperature ...
-
bioRxiv - Biophysics 2019Quote: Samples for cryo-EM were prepared by applying 4 µl of purified TRPA1 to 1.2/1.3 holey carbon grids (Quantifoil) and blotting for 8-12 sec ...
-
bioRxiv - Biophysics 2021Quote: ... 4 µL of aldolase sample were applied on UltrAuFoil R1.2/1.3 300-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly plasma cleaned for 6 seconds at 15 Watts (75% nitrogen/25% oxygen atmosphere ...
-
bioRxiv - Biophysics 2021Quote: ... 4 µL of apoferritin sample were applied on UltrAuFoil R1.2/1.3 300-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly plasma cleaned for 7 seconds at 15 Watts (75% nitrogen/25% oxygen atmosphere ...
-
bioRxiv - Biophysics 2021Quote: The LlCsm RNP and RNP-target RNA samples at approximately 0.5 mg/ml were separately applied in 4 μL volume onto glow-discharged UltrAuFoil 300 mesh R1.2/1.3 grids (Quantifoil), blotted for 3 s at 88% humidity and flash-frozen in liquid ethane using FEI Vitrobot Mark IV ...