Labshake search
Citations for Quantifoil Micro Tools :
401 - 450 of 574 citations for 5 Pyrimidinecarboxaldehyde 2 1 1 dimethylethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... 4 μl sample was applied onto Quantifoil grid Rh/Cu 200 R2/2 (Quantifoil, Inc), and was flash frozen into liquid ethane using a Vitrobot (FEI ...
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bioRxiv - Molecular Biology 2021Quote: ... and applied onto freshly glow-discharged 200 square mesh Ultrafoil R2/2 gold grids (Quantifoil). The samples were plunge-frozen with a FEI Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Freshly glow-discharged finder gold quantifoil grids (200 mesh R2/2 – Quantifoil MicroTools GmbH, Germany) were sterilized in 70% EtOH and placed in a glass-bottomed MatTek dish (MatTek Corporation ...
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bioRxiv - Microbiology 2021Quote: ... were applied to freshly washed and glow-discharged R2/2 holey carbon copper grids (Quantifoil). The grids were manually back blotted for 2 to 3s with filter paper ...
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bioRxiv - Biochemistry 2021Quote: ... Cryo-EM grids were prepared using glow discharged R2/2 Cu 200 mesh grids (Quantifoil) pre-floated onto custom-made 2.9 nm continuous carbon film ...
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bioRxiv - Cell Biology 2021Quote: ... 4 µl sample was applied onto Quantifoil grid Cu/Rh 200 R2/2 (Quantifoil, Inc), and was flash frozen into liquid ethane using a Vitrobot (FEI ...
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bioRxiv - Microbiology 2023Quote: ... sample aliquots of 3 µL were applied onto UltrAuFoil R2/2 grids (300 mesh, Quantifoil). Grids were blotted for 1 s at 10°C with 100% relative humidity and immediately plunge frozen into liquid ethane using a Vitrobot Mark IV system (TFS) ...
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bioRxiv - Molecular Biology 2023Quote: ... 3.5 μL) were applied to precoated (2 nm) R1.2/1.3 carbon-supported copper grids (Quantifoil), blotted for 4-5 s at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... A suspension containing a mixture of 10 nm protein A coated colloidal gold (Aurion) and Ebola VLPs was applied with a volume of 3 µl on a glow discharged EM grid (Quantifoil, 200 Mesh, R2/1). The grid was blotted using No.1 Whatman paper for 3 sec from the back-side in a chamber with controlled humidity set to 70% and the temperature set to 24°C.
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bioRxiv - Microbiology 2019Quote: 3 μl of purified complex at 1 to 3.6 mg/ml were applied to glow-discharged holey carbon-coated grids (Quantifoil 300 mesh, Au R1.2/1.3). Grids were blotted for 3 s at 100% humidity at 22 °C and frozen in liquid ethane using a Vitrobot Mark IV (FEI) ...
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bioRxiv - Biochemistry 2022Quote: 4 µl aliquots of purified samples at A280 = 1 were applied onto glow-discharged holey carbon-coated grids (Quantifoil 300 mesh, Au R1.2/1.3), then adsorbed for 10 s ...
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bioRxiv - Biophysics 2020Quote: Samples were prepared for CryoEM by applying 5 μl freshly purified Cx46/50-lipid nanodisc complex (∼2.5 mg mL-1) to a glow-discharged holey carbon grid (Quantifoil R 1.2/1.3, 400 mesh) for 10 seconds ...
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Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn2+-releasing mechanismbioRxiv - Biochemistry 2022Quote: ... and 3 μl of the protein solution was applied to glow-discharged (45 s) Quantifoil R2/1 300-mesh gold grids (Quantifoil Micro Tools GmbH, Germany). The grids were blotted with standard Vitrobot filter paper for 2.5 s at 4 °C under 100% humidity and plunged into liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... 3.0 µl aliquots at a concentration of 0.87 or 1.0 mg/ml of the spikeD614G-binder sample or the spikeOmicron-binder sample were applied onto glow-discharged carbon-coated copper grids (Quantifoil R2/1, 400 mesh), blotted for 4.0-8.0 s ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μl of protein at a concentration of approximately 10 mg ml−1 were loaded onto glow-discharged holey carbon grids (Quantifoil Au 300 mesh R1.2/1.3 or Quantifoil Cu/Rh 300 mesh R1.2/1.3), after which ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μL of ∼100 nM (A260 4.0 or 6.0) mitoribosome sample was applied onto a glow-discharged (20 mA for 30 sec) holey-carbon grid (Quantifoil R2/1 copper, mesh 300) coated with continuous carbon (of ∼3 nm thickness ...
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bioRxiv - Biochemistry 2024Quote: ... Approximately 3.5 μl of the solution was applied onto freshly glow-discharged and graphene oxide-coated TEM grids (Quantifoil R2/1, Cu 200 mesh) and plunge-frozen into liquid ethane by a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... 2.8 ul of the mixture was applied onto glow discharged (5 mA, 20 sec) R1.2/1.3 holey carbon gold grids (300 mesh, Quantifoil) at 22°C and 100% humidity ...
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bioRxiv - Microbiology 2023Quote: Four microliters of purified TilA-TslA-TlaA1-TlaA2 complex at 0.5 mg ml-1 in Buffer B (Supplementary Table 5) was applied onto glow-discharged (60 s, 30 mA) 300 mesh R1.2/1.3 Au grids (Quantifoil). Grids were blotted for 3 s with blot force of +15 at 10°C and 100% humidity ...
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bioRxiv - Microbiology 2023Quote: ... 2.2µl of the purified mixture at a concentration of 0.5 mg/mL was applied to glow discharged (30 s at 25mA) grid (300 mesh 1.2/1.3, Quantifoil). The grids were blotted for 3.5 s before plunging into liquid ethane using Vitrobot MK4 (TFS ...
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bioRxiv - Biochemistry 2023Quote: ... The most concentrated fraction was spun for 5 min at 13K rpm before applying 3 µL to UltraAuFoil R1.2/1.3 gold support grids (Quantifoil). Prior to sample applications grids were glow-discharged for 3 min in Pelco easiGlow glow-discharger (Pelco ...
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bioRxiv - Biochemistry 2023Quote: A 3.5 μL concentrated SPARTA sample was loaded onto a glow discharged UltrAuFoil 300 mesh R1.2/1.3 grids (Quantifoil) using a FEI Vitrobot at 4°C and 100% humidity ...
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bioRxiv - Microbiology 2021Quote: ... 3μl was added to a glow-discharged R2/2 200 mesh holey carbon EM grid (Quantifoil) for 30 seconds and the sample was blotted for 1 second ...
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bioRxiv - Cell Biology 2022Quote: ... A volume of 2.5 μL was applied on glow-discharged UltrAuFoil Au200 R2/2 grids (Quantifoil), and excess liquid was removed by blotting for 3 s (blot force 5 ...
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bioRxiv - Biochemistry 2022Quote: ... 3 µL of purified PPSA was applied to glow-discharged holy grids (Quantifoil, Cu200, R2/2) for 30s ...
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bioRxiv - Biochemistry 2023Quote: The final high resolution data set was collected across 2 grids (Quantifoil 1.2/1.3 200 mesh) prepared with 1.8 mg mL-1 trimeric complex and 0.5 and 0.1 X CMC CHAPS ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantifoil R1.2/1.3 400-square mesh copper grids with a 2 nm continuous carbon layer (Quantifoil) were plasma-treated in a Pelco easiGlow glow discharging system (Ted Pella ...
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bioRxiv - Biophysics 2020Quote: Three microliters of the apoferritin samples with 1.5 mg/ml or 0.2 mg/ml were applied onto glow-discharged 200-mesh R2/1 Quantifoil grids (used for the K3 dataset) or 200-mesh R2/1 Quantifoil grids coated with continuous carbon film (Quantifoil, used for the Falcon4 dataset), respectively ...
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bioRxiv - Biophysics 2022Quote: Cryo-EM grids were prepared by applying 3 μL of sample (at the native 1 nM concentration) on glow discharged holey gold grids (Quantifoil Cu 1.2/1.3 400 mesh). Excess sample was removed by blotting with filter paper for 4 sec prior to plunge-freezing in liquid ethane using a FEI Vitrobot Mark IV at 100% humidity and 4 °C ...
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bioRxiv - Biophysics 2019Quote: ... 3.5 μl aliquots of the recombinant human TIM22 complex (7 mg ml-1) were dropped onto glow discharged holey carbon grids (Quantifoil Au R1.2/1.3, 300 mesh), blotted with a Vitrobot Mark IV (ThemoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: Cryo-EM grids were prepared by applying 3 µL sample (at a protein concentration of 1 mg/ml) on glow-discharged holey gold grids (Quantifoil Au 1.2/1.3 300 mesh). Excess sample was removed by blotting with filter paper for 2-3 s before plunge-freezing in liquid ethane (cooled to −181 °C ...
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bioRxiv - Physiology 2021Quote: ... Aliquots of 1.25 – 1.5 μl RyR1-Nanodisc were applied onto each side of glow-discharged UltraAufoil −1.2/1.3 holey-gold (Quantifoil, Germany) with 300 TEM mesh ...
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bioRxiv - Cell Biology 2019Quote: ... Liquid culture (5 μL, ~1000 cells/μL) was blotted onto carbon-coated 200-mesh copper grids (Quantifoil Micro Tools), which were then plunged into a liquid ethane-propane mixture using a Vitrobot Mark 4 (FEI Thermo Fisher) ...
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bioRxiv - Biochemistry 2021Quote: Three microliters of the h20S-PA26E102A-Opt5 solution were applied to R2/2 400-mesh grids (Quantifoil) that had been plasma treated for 25 s using a glow discharger (Electron Microscopy Sciences ...
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bioRxiv - Molecular Biology 2022Quote: ... 3.5 μL was applied to freshly glow-discharged Quantifoil R2/2 300-square-mesh gold grids (Quantifoil) in a Vitrobot IV (ThermoFisher ...
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bioRxiv - Biophysics 2020Quote: ... 2 μl apoferritin was applied to glow-discharged gold holey carbon 1.2/1.3 300-mesh grids (Quantifoil). Grids were blotted for 4 seconds at -2 force and vitrified in liquid ethane using a MarkIV Vitrobot (ThermoFisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... 3.5 μL was applied to freshly glow discharged Quantifoil R2/2 300-square-mesh gold grids (Quantifoil) in a Vitrobot IV (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... The samples were applied to a Quantifoil R2/2 Cu 300 mesh grid (Quantifoil Micro Tools GmbH), which had been freshly glow-discharged for 60 s at 10 mA using PIB-10 (Vacuum Device) ...
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bioRxiv - Cell Biology 2024Quote: EM support grids (Au grids 200 mesh, carbon or SiO2 foil, R2/2 or R1/4, Quantifoil) were glow discharged with a Pelco easiGlow for 90 sec at 15 mA ...
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bioRxiv - Biochemistry 2021Quote: ... 3.5 μl of the freshly purified sample from the peak fraction in DDM at ~2.0 mg/ml for the B.1.1.7 protein or ~1.5 mg/ml for the B.1.351 protein was applied to a 1.2/1.3 Quantifoil grid (Quantifoil Micro Tools GmbH), which had been glow discharged with a PELCO easiGlow™ Glow Discharge Cleaning system (Ted Pella ...
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bioRxiv - Cell Biology 2023Quote: ... and 5% (w/v) glycerol was applied to a Quantifoil 300-mesh R1.2/1.3 grid (Quantifoil, Micro Tools GmbH, Germany), which had been pre-treated with glow discharge using Pelco EasiGlow immediately before use ...
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bioRxiv - Biophysics 2023Quote: 3.5 µL of protein or lipid solution was applied onto a freshly glow-discharged carbon-coated copper mesh grid (Quantifoil). After 1 min ...
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bioRxiv - Biochemistry 2023Quote: 3.5 µL of sample were applied to a freshly glow discharged 200-mesh Au R1.2/1.3 grid (Quantifoil Micro Tools), incubated for 5 s ...
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bioRxiv - Microbiology 2024Quote: The total 2.5 µL sample was applied to both sides of glow-discharged 400-mesh R1.2/1.3 Cu grids (Quantifoil, Jena, Germany). The grids were blotted for 14 s at a humidity of 100% at 8 °C and rapidly frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 µL sample was applied to glow-discharged gold holey carbon 1.2/1.3 300-mesh grids (Quantifoil) coated in-house with graphene oxide55-57 ...
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bioRxiv - Biochemistry 2021Quote: ... A volume of 2.5 μL of each sample was applied on glow-discharged UltrAuFoil Au300 Rl.2/1.3 grids (Quantifoil), and excess liquid was removed by blotting for 3 s (blot force 5 ...
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bioRxiv - Biochemistry 2022Quote: ... 2 μl retromer at a concentration between 80-100 μg/ml was applied to freshly glow discharged CF-2/2-2C C-Flat grids (Protochips, Morrisville, NC) or Quantifoil R 1.2/1.3 300 mesh copper grids (Quantifoil Micro Tools GmbH ...
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bioRxiv - Microbiology 2022Quote: ... 3.5 μL of cell suspension was applied on glow-discharged copper or gold EM grids (R2/2, Quantifoil) and automatically back-blotted (Weiss et al 2017 ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 μl of the resultant mixture was applied to glow-discharged (EasiGlow Pelco) R2/2 Quantifoil grids (Quantifoil). Excess sample was blotted away with a FEI Vitrobot (IV ...
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bioRxiv - Molecular Biology 2019Quote: ... 3 μl of the sample at 2 mg/ml were applied to 300 mesh UltrAuFoil Holey Gold Films R1.2/1.3 (Quantifoil) that had been previously plasma cleaned in a 75% argon / 25% oxygen atmosphere at 15 Watts for 6 seconds using a Solarus plasma cleaner (Gatan ...