Labshake search
Citations for Quantifoil Micro Tools :
1 - 50 of 53 citations for 2'3' cyclic GAMP cGAMP ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... by shaking at 1200 rpm for 5 minutes at 37°C using a plate shaker (Quantifoil Instruments) after stimulating with collagen at a range of concentrations ...
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bioRxiv - Bioengineering 2019Quote: ... 5 μl of the sample was placed on a carbon coated grid (Quantifoil, D) previously glow-discharged for 30 seconds (Emitech K100X ...
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bioRxiv - Biophysics 2019Quote: ... Approximately 5 µL of sample (diluted if necessary) was placed on a copper grid (Quantifoil® S7/2 Cu 400 mesh ...
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bioRxiv - Immunology 2023Quote: ... The plate was then mixed (200rpm; 37°C) for 20 minutes in the dark (BioShake iQ, Quantifoil Instruments GmbH). Samples were fixed with 1% formalin and run on the Cytek Aurora 5-laser flow cytometer (Cytek Biosciences) ...
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bioRxiv - Cell Biology 2021Quote: ... Aggregation was stimulated with TRAP-6 (25μM) in a 96-well plate (15 minutes, 750rpm, 37°C; BioShake IQ, Quantifoil Instruments) and fixed with 1% formalin (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... and 5 μl of the washed cells were introduced to Au grids (R2/1, 200 mesh; Quantifoil). The grids were fixed in 4% PFA (in TBS ...
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bioRxiv - Cell Biology 2022Quote: Vero-E6 were seeded on holey carbon film R 5/20 gold 200 mesh EM grids (Quantifoil Micro Tools GmbH ...
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bioRxiv - Microbiology 2022Quote: ... 2.7 μl of freshly purified sample was applied onto glow-discharged (30 s, 5 mA) grids (Quantifoil R 0.6/1 300 mesh Cu or Ultrafoil 0.6/1 300 mesh Au ...
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bioRxiv - Biochemistry 2023Quote: 3.5 μL of LRRK2RCKW:E11 complex was applied to glow-discharged UltraFoil 300 mesh R1.2/1.3 grids (Quantifoil) and incubated in a FEI Vitrobot IV at 4°C and 95% humidity for 20 sec ...
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bioRxiv - Biophysics 2021Quote: ... 2.8 ul of the mixture was applied onto glow discharged (5 mA, 20 sec) R1.2/1.3 holey carbon gold grids (300 mesh, Quantifoil) at 22°C and 100% humidity ...
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bioRxiv - Microbiology 2022Quote: ... the amoebae (5×105 per well) were seeded onto EM gold finder grids (Au NH2 R2/2, Quantifoil) and incubated for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... The most concentrated fraction was spun for 5 min at 13K rpm before applying 3 µL to UltraAuFoil R1.2/1.3 gold support grids (Quantifoil). Prior to sample applications grids were glow-discharged for 3 min in Pelco easiGlow glow-discharger (Pelco ...
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bioRxiv - Microbiology 2023Quote: Four microliters of purified TilA-TslA-TlaA1-TlaA2 complex at 0.5 mg ml-1 in Buffer B (Supplementary Table 5) was applied onto glow-discharged (60 s, 30 mA) 300 mesh R1.2/1.3 Au grids (Quantifoil). Grids were blotted for 3 s with blot force of +15 at 10°C and 100% humidity ...
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bioRxiv - Biochemistry 2023Quote: A 3.5 μL concentrated SPARTA sample was loaded onto a glow discharged UltrAuFoil 300 mesh R1.2/1.3 grids (Quantifoil) using a FEI Vitrobot at 4°C and 100% humidity ...
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bioRxiv - Microbiology 2023Quote: ... 2.2µl of the purified mixture at a concentration of 0.5 mg/mL was applied to glow discharged (30 s at 25mA) grid (300 mesh 1.2/1.3, Quantifoil). The grids were blotted for 3.5 s before plunging into liquid ethane using Vitrobot MK4 (TFS ...
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bioRxiv - Microbiology 2021Quote: ... and 5 µl of the mixture was placed onto freshly glow discharged holey carbon grids (R 2/1, Quantifoil) for 1 min ...
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bioRxiv - Physiology 2021Quote: ... Aliquots of 1.25 – 1.5 μl RyR1-Nanodisc were applied onto each side of glow-discharged UltraAufoil −1.2/1.3 holey-gold (Quantifoil, Germany) with 300 TEM mesh ...
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bioRxiv - Cell Biology 2019Quote: ... Liquid culture (5 μL, ~1000 cells/μL) was blotted onto carbon-coated 200-mesh copper grids (Quantifoil Micro Tools), which were then plunged into a liquid ethane-propane mixture using a Vitrobot Mark 4 (FEI Thermo Fisher) ...
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bioRxiv - Biochemistry 2022Quote: ... An aliquot of 3.5 μl of sample was placed on the glow-discharged cryo-EM grid (Quantifoil R1.2/1.3 or Quantifoil R2/1), blotted and plunge-frozen in liquid ethane using a Mark VI Vitrobot instrument maintained at 100% humidity with blot force 20 and blot time of 3 seconds ...
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bioRxiv - Biochemistry 2021Quote: ... 3.5 μl of the freshly purified sample from the peak fraction in DDM at ~2.0 mg/ml for the B.1.1.7 protein or ~1.5 mg/ml for the B.1.351 protein was applied to a 1.2/1.3 Quantifoil grid (Quantifoil Micro Tools GmbH), which had been glow discharged with a PELCO easiGlow™ Glow Discharge Cleaning system (Ted Pella ...
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bioRxiv - Biochemistry 2022Quote: ... Three μL of sample at 5 μM was adsorbed onto glow-discharged copper Quantifoil R2/1 holey carbon grids (Quantifoil). Grids were glow discharged 45 seconds at 25 mA using an EMITECH K950 apparatus ...
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bioRxiv - Cell Biology 2023Quote: ... and 5% (w/v) glycerol was applied to a Quantifoil 300-mesh R1.2/1.3 grid (Quantifoil, Micro Tools GmbH, Germany), which had been pre-treated with glow discharge using Pelco EasiGlow immediately before use ...
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bioRxiv - Biophysics 2023Quote: 3.5 µL of protein or lipid solution was applied onto a freshly glow-discharged carbon-coated copper mesh grid (Quantifoil). After 1 min ...
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bioRxiv - Biochemistry 2023Quote: 3.5 µL of sample were applied to a freshly glow discharged 200-mesh Au R1.2/1.3 grid (Quantifoil Micro Tools), incubated for 5 s ...
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bioRxiv - Microbiology 2024Quote: The total 2.5 µL sample was applied to both sides of glow-discharged 400-mesh R1.2/1.3 Cu grids (Quantifoil, Jena, Germany). The grids were blotted for 14 s at a humidity of 100% at 8 °C and rapidly frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... 5 µl of purified pelleted EVs in HBSS were applied to carbon-coated copper grids (R1.2/1.3, Quantifoil Micro Tools GmbH) and the excess of liquid was blotted automatically for two seconds from the reverse side of the grid with a strip of filter paper ...
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bioRxiv - Biophysics 2019Quote: 3 µL of purified aldolase (1.6 mg/mL) or apoferritin (5 mg/mL) was dispensed on UltrAuFoil R1.2/1.3 300-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly plasma cleaned for six seconds at 15Watts (75% nitrogen/25% oxygen atmosphere ...
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bioRxiv - Biochemistry 2023Quote: 3 μl of 50 μM purified insulin in 20 mM sodium phosphate buffer containing (pH 7.5) containing 100 mM NaCl was applied onto a grow discharged Quantifoil R1.2/1.3 Cu 200 grid (Quantifoil Micro Tool GmbH) and subsequently blotted by filter papers and plunged into liquid ethane for the vitrification using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 25 mM Tris at pH 8.0 to a concentration of 2 mg/mL to a glow-discharged (1 min at 5 mA) holey carbon grid (Quantifoil Q225-CR1.3). This was followed by plunge-freezing in liquid ethane using an FEI Vitribot Mark IV (100% humidity ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated at 37°C and 5% CO2 for 16h prior to trypsinization and seeding onto 200 mesh gold holey carbon grids (R2/2-2C; Quantifoil Micro Tools), which were placed in 3D printed grid holders [39] ...
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bioRxiv - Biophysics 2021Quote: ... 3.5 µl sample applied to a glow-discharged Quantifoil R1.2/1.3 400-mesh gold holey carbon grid (Quantifoil, Micro Tools GmbH, Germany), blotted under 100% humidity at 4 °C and plunged into liquid ethane using a Mark IV Vitrobot (FEI) ...
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bioRxiv - Microbiology 2021Quote: A 2.5-ml suspension of the purified medusavirus particles was applied to a quantifoil grid (R1.2/1.3 Mo) (Quantifoil Micro Tools GmbH, Germany), which was glow-discharged beforehand for 30 s ...
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... A 3.5 µL virus sample solution was applied onto a glow discharged copper grid coated with holey carbon (R2/1; Quantifoil, Jena, Germany) prior to plunge-freezing ...
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bioRxiv - Cell Biology 2022Quote: ... 3.5 µL of sample were applied to glow discharged (45s 30 mA) 1.2/1.3 Ultrafoil holey grids (Quantifoil Micro Tools GmbH, Germany) and they were plunged frozen in liquid ethane with a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... 3.5 uL of sample was applied at a concentration of 3-5 mg/mL to glow-discharged holey carbon grids (Quantifoil R1.2/1.3). The grids were blotted using an FEI Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... 3.5 μL sample with addition of 0.05% β-OG was directly applied to glow-discharged 200 mesh gold grids (Quantifoil R1.2/1.3) and vitrified using a FEI Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: Farnesylated GBP1 reconstituted on liposomes (as described above) were supplemented with 5 nm colloidal gold and applied on glow-discharged UltrAuFoil R 2/2 gold grids (Quantifoil Micro Tools), flash-frozen in liquid ethane using a Vitrobot Mark II device (FEI) ...
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bioRxiv - Cell Biology 2024Quote: ... we micropatterned squares and circles varying from 5 to 30 μm across on 300 mesh gold grids coated with a thin perforated layer of silicon dioxide (Quantifoil R1/4). The grids were exposed to atmospheric plasma ...
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bioRxiv - Cell Biology 2024Quote: ... An aliquot of a 5 μl sample was applied to a glow-discharged holey carbon grid (Quantifoil R2/2, Au, 400 mesh). After waiting for 60 s ...
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bioRxiv - Biophysics 2021Quote: To provide extra support for cell growth 5-6 nm of carbon was evaporated onto Quantifoil Au 200 R2/1 or R2/2 grids (Quantifoil Micro Tools GmbH). Grids were then glow discharged and incubated in supplemented RPMI-1640 overnight in the cell incubator ...
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bioRxiv - Biophysics 2022Quote: Purified wt or mutant D263A or F55A MVMp capsid samples (5 μl) were applied onto R2/2 300 mesh copper grids (Quantifoil Micro Tools, Germany) and vitrified using a Leica EM CPC cryofixation unit ...
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bioRxiv - Biochemistry 2022Quote: ... or AE1-Niflumic acid at 5 mg/ml were applied to glow-discharged holey carbon EM grids (Quantifoil 300 copper mesh, R1.2/1.3) in an EM-GP2 plunge freezer (Leica) ...
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bioRxiv - Biochemistry 2021Quote: ... 3 μL of protein solution at 5 mg/mL (with 0.025% DDM) was applied onto a glow-discharged holey carbon grid (Quantifoil, 300 mesh, R1.2/1.3). Excess samples were blotted for 5.0 s with a blotting force of 3 ...
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bioRxiv - Biophysics 2021Quote: ... 2.5 μL of each purified CCKAR–G protein complex was applied individually onto the glow-discharged holey carbon grids (Quantifoil, Au300 R1.2/1.3) in a Vitrobot chamber (FEI Vitrobot Mark IV) ...
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bioRxiv - Molecular Biology 2022Quote: The purified complex samples (3 μL at 5-8 mg/mL) were applied to glow-discharged holey grids (Quantifoil R1.2/1.3, 300 mesh) and subsequently vitrified using a Vitrobot Mark IV (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... 5 µL of the mixture was placed onto freshly glow discharged holey carbon grids (Quantifoil™ R 2/1 on 200 copper mesh) for 1 minute ...
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bioRxiv - Biochemistry 2024Quote: ... 3.5 μL of this protein-DNA mix were applied to 300-mesh copper R2/1 Quantifoil Holey Carbon Grids (Quantifoil Micro Tools GmbH) that had been freshly glow discharged for 30 seconds at 25 mA in a GloQube Glow Discharge System (Quorum Technologies ...
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bioRxiv - Microbiology 2021Quote: ... 5 µl of the cell suspension was placed on freshly glow-discharged (for ∼25 s) holey carbon grids (Quantifoil Cu R2/1, 200 mesh). The grids were front blotted with Whatman filter paper and rapidly frozen in liquid ethane ...
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bioRxiv - Biophysics 2021Quote: ... 3 μL of the purified lasmiditan–5-HT1F–Gi complex at concentration ∼15 mg/ml were applied onto a glow-discharged holey carbon grid (Quantifoil R1.2/1.3 Au 300). Grids were plunge-frozen in liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... 3 μL of purified mGlu5 (with the different ligands) at 5 mg/ml was applied on glow-discharged holey carbon gold grids (Quantifoil R1.2/1.3, 200 mesh). The grids were blotted using a Vitrobot Mark IV (FEI ...