Labshake search
Citations for Quantifoil Micro Tools :
401 - 450 of 677 citations for 2 Methyl 4 piperidin 1 ylsulfonyl phenylboronic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The mixture was added onto glow-discharged copper R2/2 Quantifoil holey carbon grids (Quantifoil Micro Tools GmbH, Jena, Germany). Grids were blotted for 6-8 seconds (under 100% humidity conditions ...
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bioRxiv - Biochemistry 2024Quote: ... was applied to glow-discharged (GloQube® Plus, Quorum, UK) Quantifoil R1.2/1.3 or R2/2 mesh 200 copper holey carbon grid (Quantifoil, Germany), blotted under 100% humidity at 4°C for 6–8 s ...
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bioRxiv - Cell Biology 2024Quote: HeLa WT cells or HeLa cells stably expressing Apaf1-GFP were plated on 200 mesh gold EM grids with a holey carbon film R2/2 (Quantifoil). HeLa WT cells were transfected with Apaf1-SNAP-tag ...
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bioRxiv - Microbiology 2024Quote: ... A 4.5 µL droplet of the culture was applied to glow discharged copper grids (Quantifoil R2/2, Quantifoil Micro Tools) and blotted for 2.5 s in a 95% humidity chamber at 20 °C ...
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bioRxiv - Microbiology 2024Quote: ... We initially prepared frozen-hydrated viruses deposited onto Quantifoil R2/2 or R1.2/1.3 grids (Quantifoil Micro tools GmbH, Germany) or glow discharged Lacey grid (Ted Pella Inc. ...
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bioRxiv - Neuroscience 2024Quote: Plunge-freezing VLPs: 3 µL of crude enriched VLP resuspension was applied to Holey copper R2/2 grids (Quantifoil GmBH) glow-discharged at 5mA 30s using an EasiGlow system (Pelco) ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µL of 10 µM 11-16Ub[K48]-GFP-35 was pipetted on a holey carbon R2/2 Cu200 grid (Quantifoil) that was plasma cleaned by a SCD005 sputter coater (Bal-Tec ...
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bioRxiv - Cell Biology 2024Quote: ... 3 µL of LRRK2RCKW:RN277:E11 DARPin complex were applied to a glow-discharged UltraAuFoil Holey Gold 200 mesh R2/2 grid (Quantifoil, Q250AR2A) and incubated in a FEI Vitrobot IV chamber at 4 °C and 95 % humidity for 20 sec ...
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bioRxiv - Biochemistry 2024Quote: HeLa cells stably expressing either HRS-mScarlet or HRS-mCherry were grown on 200 mesh gold EM grids with a holey carbon film R2/2 (Quantifoil). Cells were incubated with 50 ng/ml EGF- Alexa 647 ...
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bioRxiv - Biophysics 2020Quote: ... An aliquot of 4 μl pY39 α-syn fibril solution was applied to a glow-discharged carbon electron microscope grid (Quantifoil R1.2/1.3, 300 mesh). After 10 s of incubation ...
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bioRxiv - Molecular Biology 2021Quote: The purified GIP1-42–GIPR–Gs–Nb35 complex at a concentration of 6-7 mg/mL was mixed with 100 μM GIP1-42 at 4 C and applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, Au 300 mesh) that were subsequently vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: The purified tirzepatide–GIPR–Gs–Nb35 complex at a concentration of 18-20 mg/mL was mixed with 100 μM tirzepatide at 4°C and applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, Au 300 mesh) that were subsequently vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: The purified non-acylated tirzepatide–GIPR–mini-Gs–Nb35 complex at a concentration of 14-16 mg/mL was mixed with 100 μM non-acylated tirzepatide at 4°C and applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, Au 300 mesh) that were subsequently vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: The purified peptide 20–GIPR–Gs–Nb35 complex at a concentration of 5-6 mg/mL was mixed with 100 μM peptide 20 at 4°C and applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, Au 300 mesh) that were subsequently vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: The purified peptide 20–GCGR–Gs–Nb35 complex at a concentration of 12-14 mg/mL was mixed with 100 μM peptide 20 at 4°C and applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, Au 300 mesh) that were subsequently vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... Four microliters of purified Cx36 proteins (4-6 mg/ml) were applied onto a negatively glow-discharged holey carbon grid (Quantifoil R1.2/1.3 Cu 300 mesh). For samples involving Cx36-WT ...
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bioRxiv - Biochemistry 2022Quote: ... R3.5/1 Cu 200 grids (Quantifoil) were glow-discharged on a glass clover slide for 60 seconds at 25 mA and plunging into liquid ethane was performed using an EM GP (Leica Microsystems) ...
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bioRxiv - Neuroscience 2020Quote: Holey carbon grids (Quantifoil R2/1) covered with either 2 nm amorphous carbon (Quantifoil ...
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bioRxiv - Cell Biology 2023Quote: Quantifoil R3.5/1 Au200 grids (Quantifoil) were glow discharged for 30 s in an Auto306 Edwards Turbo Coater at power 6 (20 mA ...
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bioRxiv - Biochemistry 2024Quote: ... Grids (Quantifoil UltrAuFoil R 0.6/1) were glow-discharged (20 mA ...
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bioRxiv - Biophysics 2020Quote: ... samples were stored on ice and applied to the cryo-grids (2 nm precoated Quantifoil R3/3 holey carbon supported grids) at a concentration of 10 μM and vitrified using a Vitrobot Mark IV (FEI) ...
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bioRxiv - Plant Biology 2021Quote: ... 3 µl of suspended cells in culture medium were applied to the front side of glow-discharged R2/2 carbon-coated copper grids (Quantifoil MicroTools), with additional 1 µL cells added to the back side ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of the sample mixture was applied to a freshly glow-discharged copper R2/2 200 grid (Quantifoil Micro Tools), pre-blotted for 30 seconds ...
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bioRxiv - Biochemistry 2021Quote: ... Three microliters of the protein solution were applied on a glow-discharged holey carbon grids (200 mesh Quantifoil R2/2 molybdenum), which had been treated with H2 and O2 mixtures in a Solarus plasma cleaner (Gatan ...
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bioRxiv - Cell Biology 2022Quote: ... The IL-11Δ10/IL-11RαD1-D3/gp130D1-D3 complex (0.5 mg/mL) was blotted onto UltrAuFoil grids (R2/2, Quantifoil Micro Tools GmbH) that had been subjected to glow discharge (15 mA for 30 s) ...
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bioRxiv - Biophysics 2023Quote: ... 3 to 3.5 μl of complex was applied to a glow-discharged UltrAuFoil Holey Gold 200 mesh R2/2 grid (Quantifoil, Catalog # Q250AR2A) and incubated in a FEI Vitrobot IV chamber at 4°C and 95% humidity for 20 sec ...
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bioRxiv - Biochemistry 2024Quote: ... 3 µL of suspended cells in culture medium were applied to the front side of glow-discharged R2/2 carbon-coated copper grids (Quantifoil MicroTools), with additional 1 µL cells added to the back side ...
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bioRxiv - Cancer Biology 2024Quote: ... or 0.5 mg/ml of RanGTP•selinexor-XPO1•ASB8ΔN16•ELOB/C supplemented with 2-fold excess ASB8ΔN16-ELOB/C and selinexor) was applied to a 400-mesh copper grid (Quantifoil R1.2/1.3). For unliganded XPO1 ...
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bioRxiv - Biophysics 2020Quote: ... The WT and Y222F MTs were applied to lacey carbon grids (Agar Scientific AGS166-4 400 mesh copper grids) and holey carbon grids (Quantifoil R1.2/1.3 300 mesh copper grids) immediately after application of glow discharge to the grids ...
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bioRxiv - Biophysics 2021Quote: 3 μl of the human sperm sample were applied to glow discharged copper R2/2 200-mesh holey carbon grid (Quantifoil Micro Tools) and plunge frozen in liquid ethane using an automatic plunge freezer (Vitrobot ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated at 37°C and 5% CO2 for 16h prior to trypsinization and seeding onto 200 mesh gold holey carbon grids (R2/2-2C; Quantifoil Micro Tools), which were placed in 3D printed grid holders [39] ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 μL aliquots of the cell suspension were applied to glow-discharged R2/2 200 mesh copper Quantifoil grids (Quantifoil Micro Tools), the sample was pre-blotted for 30 seconds ...
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bioRxiv - Molecular Biology 2021Quote: ... After another 3 min at 37 °C the termination complexes were placed on ice for 15 min and subsequently applied to Quantifoil R2/2 holey carbon grids (Quantifoil Micro Tools) either coated with a thin continuous carbon film or with graphene oxide flakes (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... 4 µl of the solution was applied to a glow-discharged (30s at 35 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools). After removing excess liquid by blotting the grid from the back side with Whatman filter paper for 2 s ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were applied to freshly glow-discharged (Edwards S150B, 45 sec, 30-50 mA, 1,2kV, 10-2 mbar) copper holey carbon grids (Quantifoil, Cu-R1.2/1.3) under 100% humidity ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μl of the mixture was deposited on glow discharged R2/2 Copper 300 mesh holey carbon-coated support grids (Quantifoil, Jena, Germany) along with 1 μl of HEPES pH 10 and 1 μl of hydrogen peroxide 0.9 % ...
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bioRxiv - Neuroscience 2021Quote: ... 400 mesh Quantifoil R1.2/1.3 grids with empty holes and 400 mesh Quantifoil R1.2/1.3 grids coated with a 2 nm thin carbon layer (Quantifoil Micro Tools GmbH, Germany). The grids were rendered hydrophilic by glow discharge using a Pelco easiGlow glow discharger (Ted Pella Inc. ...
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bioRxiv - Microbiology 2020Quote: ... Cells were concentrated by centrifugation and 3 μL aliquots of the cell suspension are applied to glow-discharged R2/2 200 mesh copper Quanti-foil grids (Quantifoil Micro Tools), the sample was pre-blotted for 30 seconds ...
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bioRxiv - Cell Biology 2020Quote: ... A 5 µl drop of the chemically fixed cell culture was deposited on a glow-discharged Quantifoil 200 mesh R2/2 electron microscopy grid (Quantifoil, Großlöbichau, Germany) pre-coated with a gold bead solution ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µL aliquots of 0.1 mg/ml cross-linked ternary complexes were applied to freshly glow-discharged R2/2 Quantifoil 200 mesh copper grids (Quantifoil Micro Tools). Grids were blotted in a climate chamber set to 20 °C and at 95% humidity before plunge frozen in liquid ethane set at −183 °C using a Leica EM GP system (Leica Microsystems) ...
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Computational toolbox for ultrastructural quantitative analysis of filament networks in cryo-ET databioRxiv - Cell Biology 2021Quote: B16-F1 cells were cultured as described above and seeded onto 200 mesh gold holey carbon grids (R2/2-2C; Quantifoil Micro Tools). Prior to cell seeding ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μl of the resulting mixture was spotted onto glow-discharged copper EM grids (Quantifoil 300 Au mesh, R2/2 carbon film (Quantifoil Micro Tools). The grids were then blotted for 2 or 4 seconds (blot force −10 ...
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bioRxiv - Cell Biology 2022Quote: ... 3 µl of the microtubule sample was deposited at the surface of a glow-discharged holey carbon grid (Quantifoil R2/2, Cu200) in the temperature (35 °C ...
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bioRxiv - Cell Biology 2024Quote: ... An aliquot of a 5 μl sample was applied to a glow-discharged holey carbon grid (Quantifoil R2/2, Au, 400 mesh). After waiting for 60 s ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were applied to freshly glow-discharged (Edwards S150B, 45 s, 30−50 mA, 1.2 kV, 10−2 mbar) copper holey carbon grids (Quantifoil, Cu-R1.2/1.3) under 100% humidity at 4°C.
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bioRxiv - Biochemistry 2023Quote: 4.5 µL of freshly mixed Nup98 condensate and capsid mixture with protein A-gold (10 nm) were applied onto a glow-discharged Quantifoil R2/2 copper grid (Quantifoil Micro Tools). The grid was blotted in the front for 2.5 s at 15 °C with 90% relative humidity and then plunged into liquid ethane using a Lecia EM GP device (Lecia Microsystem) ...
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bioRxiv - Biophysics 2023Quote: ... Micropatterns covered an area of 8 × 8 squares around the center of the cryo-TEM grids (Quantifoil R1/4 or R1/20, Au 200 mesh, SiO2 film) as previously described31 ...
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bioRxiv - Biochemistry 2021Quote: UltrAuFoil 0.6/1 300 mesh grids (Quantifoil) were glow discharged in residual air at 25 mA for 3 min using a Pelco EasiGLOWTM ...
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bioRxiv - Biochemistry 2022Quote: UltrAuFoil® gold grids (0.6/1, Quantifoil) (Russo and Passmore ...
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bioRxiv - Biochemistry 2023Quote: ... UltrAuFoil 0.6/1 300-mesh grids (Quantifoil) were glow discharged in residual air at 20LmA for 60 s each side using a Pelco EasiGLOW ...