Labshake search
Citations for Quantifoil Micro Tools :
401 - 450 of 560 citations for 2 Amino 3 chloro 5 nitro 6 picoline since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: Samples were concentrated to 0.2 mg/ml or 1 mg/ml and prepared either on copper grids with holey carbon support and an additional layer of 2 nm continuous carbon (R1.2,1.3, Quantifoil) or on UltAufoil grids (R0.6/1.0 ...
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bioRxiv - Biochemistry 2022Quote: ... Several serial dilutions were prepared and 3 µl of each sample was loaded on Quantifoil R2/2Cu/Rh 300 holey-carbon-supported grids (Quantifoil Micro Tools GmbH, Jena, Germany). Initial cryo-EM sample preparations showed clear protein aggregation ...
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bioRxiv - Biophysics 2022Quote: Cryo-EM grids were prepared by applying 3 μL of sample (at the native 1 nM concentration) on glow discharged holey gold grids (Quantifoil Cu 1.2/1.3 400 mesh). Excess sample was removed by blotting with filter paper for 4 sec prior to plunge-freezing in liquid ethane using a FEI Vitrobot Mark IV at 100% humidity and 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: Cryo-EM grids were prepared by applying 3 µL sample (at a protein concentration of 1 mg/ml) on glow-discharged holey gold grids (Quantifoil Au 1.2/1.3 300 mesh). Excess sample was removed by blotting with filter paper for 2-3 s before plunge-freezing in liquid ethane (cooled to −181 °C ...
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bioRxiv - Immunology 2020Quote: An aliquot of 3 μl purified protein of rNLRP1-rDPP9 or rNLRP1FIIND S969A-rDPP9 complex was applied to holey carbon grids (Quantifoil Au 1.2/1.3, 300 mesh), which were glow-discharged for 30 s at middle level in Harrick Plasma after 2 min evacuation ...
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bioRxiv - Biophysics 2023Quote: 2.5μL aliquots of Fks1 at a concentration of about 3 mg/mL were placed on glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3, 300 mesh) and were flash-frozen in liquid ethane using a FEI Vitrobot Mark IV ...
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bioRxiv - Molecular Biology 2023Quote: 3 µL of the human CDCA7264-371 in complex with the nucleosome harboring a single hemimethylated CpG in the 3’-linker DNA was applied onto the glow-discharged holey carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh). The grids were plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: Vitrified grids were prepared by applying 3 µl of 1 uM complex sample to a freshly glow- discharged holey carbon grids (Quantifoil R1.2/1.3 Au 200 mesh). The sample was blotted for 3-5 s at 4 ℃ with 100% humidity and plunged-frozen in liquid nitrogen cooled ethane (FEI TM ...
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bioRxiv - Biochemistry 2022Quote: ... An aliquot of 3.5 μl of sample was placed on the glow-discharged cryo-EM grid (Quantifoil R1.2/1.3 or Quantifoil R2/1), blotted and plunge-frozen in liquid ethane using a Mark VI Vitrobot instrument maintained at 100% humidity with blot force 20 and blot time of 3 seconds ...
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bioRxiv - Biochemistry 2021Quote: ... 3.5 μl of the freshly purified sample from the peak fraction in DDM at ~2.0 mg/ml for the B.1.1.7 protein or ~1.5 mg/ml for the B.1.351 protein was applied to a 1.2/1.3 Quantifoil grid (Quantifoil Micro Tools GmbH), which had been glow discharged with a PELCO easiGlow™ Glow Discharge Cleaning system (Ted Pella ...
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bioRxiv - Biochemistry 2022Quote: ... Three μL of sample at 5 μM was adsorbed onto glow-discharged copper Quantifoil R2/1 holey carbon grids (Quantifoil). Grids were glow discharged 45 seconds at 25 mA using an EMITECH K950 apparatus ...
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bioRxiv - Microbiology 2024Quote: The total 2.5 µL sample was applied to both sides of glow-discharged 400-mesh R1.2/1.3 Cu grids (Quantifoil, Jena, Germany). The grids were blotted for 14 s at a humidity of 100% at 8 °C and rapidly frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... and 5% (w/v) glycerol was applied to a Quantifoil 300-mesh R1.2/1.3 grid (Quantifoil, Micro Tools GmbH, Germany), which had been pre-treated with glow discharge using Pelco EasiGlow immediately before use ...
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bioRxiv - Biophysics 2023Quote: 3.5 µL of protein or lipid solution was applied onto a freshly glow-discharged carbon-coated copper mesh grid (Quantifoil). After 1 min ...
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bioRxiv - Biochemistry 2023Quote: 3.5 µL of sample were applied to a freshly glow discharged 200-mesh Au R1.2/1.3 grid (Quantifoil Micro Tools), incubated for 5 s ...
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bioRxiv - Biophysics 2024Quote: ... 3 µL of purified AE1 at 5 mg/mL was added to a glow discharged (PELCO easiGlow) 0.6/1 µm holey gold grid (Quantifoil UltrAuFoil) and blotted for 4-6 s at 4°C and 100% humidity using the Vitrobot Mark IV system (Thermofisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 µL sample was applied to glow-discharged gold holey carbon 1.2/1.3 300-mesh grids (Quantifoil) coated in-house with graphene oxide55-57 ...
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bioRxiv - Biochemistry 2021Quote: ... A volume of 2.5 μL of each sample was applied on glow-discharged UltrAuFoil Au300 Rl.2/1.3 grids (Quantifoil), and excess liquid was removed by blotting for 3 s (blot force 5 ...
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bioRxiv - Biochemistry 2022Quote: ... 2 μl retromer at a concentration between 80-100 μg/ml was applied to freshly glow discharged CF-2/2-2C C-Flat grids (Protochips, Morrisville, NC) or Quantifoil R 1.2/1.3 300 mesh copper grids (Quantifoil Micro Tools GmbH ...
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bioRxiv - Plant Biology 2022Quote: ... 2 μL of sample solution was applied onto a holey carbon grid (Quantifoil R2/1, Cu 300 mesh) covered with a thin amorphous carbon film ...
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bioRxiv - Microbiology 2022Quote: ... 3.5 μL of cell suspension was applied on glow-discharged copper or gold EM grids (R2/2, Quantifoil) and automatically back-blotted (Weiss et al 2017 ...
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bioRxiv - Evolutionary Biology 2021Quote: Molybdenum R1.2/1.3 and R2/2 200-mesh grids with a holey carbon support film (Quantifoil, Jena, DE) were glow discharged for 40 s under high vacuum shortly before sample application ...
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bioRxiv - Biochemistry 2021Quote: ... 2 uL of crystal slurry was applied to each side of a glow-discharged holey carbon grid (Quantifoil, R 2/1 200 mesh Cu ...
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bioRxiv - Biochemistry 2021Quote: ... 2 uL of crystal slurry was applied to each side of a glow-discharged holey carbon grid (Quantifoil, R 1/4 300 mesh Cu ...
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bioRxiv - Cell Biology 2020Quote: ... K735 apo and K772-ANP datasets were collected on untreated carbon/copper grids (Quantifoil R2/2 300 mesh). The grids used for the other 15 datasets were gold grids (UltrAuFoil R2/2 200 mesh) ...
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bioRxiv - Microbiology 2022Quote: Phage particles were negatively stained for 20 s with 2 % uranyl acetate on carbon-coated copper grids (Quantifoil) and observed at 100 kV on a Hitachi HT 7700 equipped with an AMT XR81B Peltier cooled CCD camera (8M pixel ...
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bioRxiv - Microbiology 2023Quote: ... Phage particles were negatively stained for 20 seconds with 2% uranyl acetate on carbon-coated copper grids (Quantifoil) and observed at 100 kV on a Hitachi HT 7700 equipped with an AMT XR81B Peltier cooled CCD camera (8M pixel ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then seeded onto gold grids (either ‘UltrAuFoil’ 200 Au mesh, R2/2 gold film, or Quantifoil 300 Au mesh ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 μl of KMN complex at 0.3 mg/ml supplemented with 0.003% Triton were applied to glow-discharged UltrAuFoil R2/2 grids (Quantifoil). Excess liquid was removed by blotting (3.5 s at blot force −3) ...
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bioRxiv - Plant Biology 2023Quote: ... H4 NCPs were applied to Quantifoil R2/1 200-mesh grids coated with 2 nm of carbon (Quantifoil). H4.VS were applied to unsupported Quantifoil R2/1 200-mesh grids (Quantifoil) ...
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bioRxiv - Biochemistry 2023Quote: ... Differentiated U937 cells became adhesive and were seeded onto gold London Finder grids (Au H2 R2/2, Quantifoil) coated with vitronectin (25 µg/mL in 1xPBS ...
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bioRxiv - Biophysics 2024Quote: ... Regions of the LCP drops were scooped and gently smeared across pre-clipped Cu200 R2:2 grids (Quantifoil) under a humidifier ...
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bioRxiv - Microbiology 2024Quote: ... 4 µl of the concentrated cells were then spotted onto a glow discharged copper grid (R2/2, Quantifoil). By using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: About 3 µl of the purified complex sample was applied to a freshly-glow discharged Au 300 mesh R1.2/1.3 grids coated with 2 nm carbon film (Quantifoil). The grids were blotted with force level of 1 and blot time of 4s ...
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bioRxiv - Microbiology 2024Quote: Cryo-ET samples were prepared using copper grids with holey carbon support film (300 mesh, R2/2, Quantifoil) as previously described116 ...
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bioRxiv - Biophysics 2024Quote: 3.5 µL of sample were applied to 2/1 Quantifoil holey carbon grids (Quantifoil Micro Tools GmbH, Germany) or 1.2/1.3 Ultrafoil holey gold grids (Quantifoil Micro Tools GmbH ...
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bioRxiv - Biophysics 2024Quote: 3 μL of concentrated protein sample was applied to a glow-discharged (30 s at 15 mA) grid (Quantifoil Au or Cu R1.2/1.3 300 mesh) and after a wait time of 5 s ...
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bioRxiv - Biochemistry 2024Quote: 3µl of the AtGDH-AKG bound binary complex (3 mg/ml) was applied to freshly glow-discharged holey carbon grids (Quantifoil Au R 0.6/1.0 300 mesh). For the apo form AnGDH dataset ...
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bioRxiv - Biochemistry 2023Quote: 3 μl of 50 μM purified insulin in 20 mM sodium phosphate buffer containing (pH 7.5) containing 100 mM NaCl was applied onto a grow discharged Quantifoil R1.2/1.3 Cu 200 grid (Quantifoil Micro Tool GmbH) and subsequently blotted by filter papers and plunged into liquid ethane for the vitrification using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... HeLa cells were grown for 24 h on holey carbon-covered gold EM grids (200 mesh, R2/2, Quantifoil), fed with oleic acid and induced with doxycycline for Fsp27-EGFP expression for an unrelated project ...
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bioRxiv - Biophysics 2021Quote: ... 4 µl of this mixture was applied on a glow-discharged Quantifoil R2/2 grid (Quantifoil Micro Tools GmbH) and flash-frozen in liquid ethane using a Vitrobot Mark II device (FEI) ...
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bioRxiv - Biophysics 2020Quote: Three microliters of purified HCoV-NL63 virions (4.5 mg/mL) were first applied to 300-mesh R2/1 + 2 nm C-film grids (Quantifoil) that had been glow-discharged for 15s PELCO easiGlow (TED PELLA ...
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bioRxiv - Biophysics 2020Quote: Cryo-EM grids were prepared by applying freshly purified channel protein (~3.5 μl) to glow-discharged copper Quantifoil R2/2 holey carbon grids (Quantifoil), which were then blotted for 2 s with ~100% humidity and flash frozen in liquid ethane using FEI Vitrobot Mark IV (FEI) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were grown on 200 mesh gold R2/2 London Finder Quantifoil grids (Quantifoil Micro Tools GmbH, Jena, Germany). Prior to seeding cells ...
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bioRxiv - Biophysics 2024Quote: ... This bleb/beads suspension was then applied onto glow discharged plasma-cleaned carbon-coated copper grids (GR1/2, Quantifoil) and manually blotted for ∼3 seconds with 55 mm Whatman filter paper (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... 4 μL of the cell mixture was applied three times onto glow-discharged copper EM grids (R2/2, Quantifoil), followed by plunge freezing into liquid ethane/propane ...
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bioRxiv - Biophysics 2024Quote: ... Aliquots of 1.25 – 1.5 ul RyR1-nanodisc were applied onto each side of glow-discharged C-Flat - 2/1 - 3Au holey-carbon (Protochips, NC) or UltraAufoil -1.2/1.3 holey-gold (Quantifoil, Germany) with 300 TEM mesh ...
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bioRxiv - Biophysics 2023Quote: ... 4 μl of 4.3 mg/ml Slo1-γ1 complex were applied to glow-discharged UltrAuFoil R2/2 200 grid (Quantifoil). Any excess liquid was removed by blotting for 3.5 s at a blot force of −3 ...
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bioRxiv - Biophysics 2023Quote: Gold 200 mesh carbon or SiO2 R 2/1 grids or R 1.2/20 grids (Quantifoil Micro Tools GmbH) were coated with ∼8 nm of carbon using a Leica ACE600 (Leica Microsystems GmbH) ...
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bioRxiv - Biophysics 2023Quote: ... using FluoSpheres (diameter of 1 μm) on gold Quantifoil grids (200 mesh, R 2/1, holey SiO2 film; Quantifoil Micro Tools GmbH ...