Labshake search
Citations for Quantifoil Micro Tools :
351 - 400 of 611 citations for N Furan 2 ylmethyl 3 bromo 4 fluoro benzamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... cells were seeded onto holey carbon gold EM grids (200 mesh, R2/2, Quantifoil) coated with 10 mg/ml fibronectin ...
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bioRxiv - Biochemistry 2021Quote: ... 3 microlitres of undiluted eluate and of eluate which was diluted 1:2 or 1:4 in buffer A200 were blotted onto holey carbon R 1.2/1.3 copper 300 mesh grids (Quantifoil) and vitrified in liquid ethane using a Vitrobot MarkIV device (Thermofisher) ...
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bioRxiv - Biophysics 2021Quote: ... 4 µL of aldolase sample were applied on UltrAuFoil R1.2/1.3 300-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly plasma cleaned for 6 seconds at 15 Watts (75% nitrogen/25% oxygen atmosphere ...
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bioRxiv - Biophysics 2021Quote: ... 4 µL of apoferritin sample were applied on UltrAuFoil R1.2/1.3 300-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly plasma cleaned for 7 seconds at 15 Watts (75% nitrogen/25% oxygen atmosphere ...
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bioRxiv - Biophysics 2021Quote: The LlCsm RNP and RNP-target RNA samples at approximately 0.5 mg/ml were separately applied in 4 μL volume onto glow-discharged UltrAuFoil 300 mesh R1.2/1.3 grids (Quantifoil), blotted for 3 s at 88% humidity and flash-frozen in liquid ethane using FEI Vitrobot Mark IV ...
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bioRxiv - Molecular Biology 2022Quote: ... for 15 min before 4 μL of sample was applied to a graphene oxide coated 1.2/1.3 UltrAuFoil grids (Quantifoil) at 4°C under 100% humidity86 ...
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bioRxiv - Microbiology 2022Quote: ... Aliquots of 4 μL samples were applied to glow-discharged holey carbon girds (Cu, R1.2/1.3, 300 mesh, Quantifoil). The grids were blotted with force 2 for 3 seconds and plunged into liquid ethane using a Vitrobot (FEI Thermo Fisher) ...
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bioRxiv - Biochemistry 2024Quote: ... 4 uL of the concentrated sample was applied to freshly glow-discharged R2/1 holey carbon grids (Quantifoil), blotted with blot force of 5 for 5 s using a Vitrobot Mark IV (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... 3.5 µl cell suspension applied on glow-discharged (Pelco easiGlow) R1/4 SiO2 gold 200 mesh grids (Quantifoil), blotted for 2 sec from the backside and was plunge-frozen on a Leica EM GP2 plunger at 20 °C ...
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A distinctive ligand recognition mechanism by the human vasoactive intestinal polypeptide receptor 2bioRxiv - Molecular Biology 2021Quote: The purified PACAP27–VIP2R–Gs complex (3 μL at 3.7 mg per mL) was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3). Vitrification was performed using a Vitrobot Mark IV (ThermoFisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Samples (3 µL) were applied to a glow-discharged UltrAuFoil R1.2/1.3 Au 300 gold foil grids (Quantifoil GmbH, Großlöbichau, Germany) and were flash frozen in liquid ethane using a Vitrobot mark IV (Thermo Fisher Scientific ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... 3 µL samples were applied to freshly glow-discharged Cu 200 Holey Carbon R1.2/1.3 grids (Quantifoil, for samples without FFosC), or Cu 200 Holey Carbon R2/1 grids (Quantifoil ...
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bioRxiv - Biochemistry 2021Quote: The purified TIP39–PTH2R–Gs complex (3 μL at 8.5 mg per mL) was applied on a glow-discharged holey carbon grid (Quantifoil R1.2/1.3). Vitrification was performed using a Vitrobot Mark IV (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The complex (3 μL) was quickly applied on a glow-discharged UltraAuFoil R1.2/1.3 300 mesh holey Au grids (Quantifoil Micro Tools GmbH), blotted with Vitrobot Mark IV (FEI) ...
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bioRxiv - Biophysics 2024Quote: Approximately 3□μL of the sample solution were put on newly glow-discharged TEM grids (Quantifoil R2/1, Cu, mesh 200) and plunge-frozen in liquid ethane using the Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Cryo-EM grids were prepared by applying 3 µl of concentrated sample onto 400-mesh R1.2/1.3 UltrAuFoil grids (Quantifoil Micro Tools GmbH), which had rendered hydrophilic by glow discharging at 15mA for 60seconds with a PELCO EasyGlow device (TED PELLA ...
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bioRxiv - Biochemistry 2022Quote: ... stock solution was mixed with 3.5 µL of the KWOCA 4 sample and 3 µl were immediately loaded onto the UltrAuFoil R 1.2/1.3 grids (Au, 300-mesh; Quantifoil Micro Tools GmbH). Prior to sample loading the grids were subjected to Ar/O2 plasma cleaning for 10 s on a Solarus 950 plasma cleaner (Gatan) ...
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bioRxiv - Neuroscience 2023Quote: ... each time was sequentially blotted 3 times to 300-mesh holey carbon Au R1.2/1.3 grids treated with 25s of glow discharge (Quantifoil, Micro Tools GmbH). Fluorinated Fos-choline-8 (0.65 mM ...
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bioRxiv - Microbiology 2024Quote: ... The sample solution (3 μl) was applied to glow-discharged (20 mAmp, 30 sec, Quorum GloQube) Quantifoil R1.2/1.3 grids (Quantifoil Micro Tools GmbH), blotted for 5 s using blot force 0 and plunge frozen into liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2024Quote: Cryo-EM grids for the SIRT7-nucleosome complexes were prepared by applying 3 μL of concentrated sample onto 400-gold mesh R1.2/1.3 Quantifoil grids (Quantifoil Micro Tools GmbH). These grids were rendered hydrophilic by glow discharging at 15 mA for 90 s with a PELCO easiGlow device (Ted Pella Inc.) ...
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bioRxiv - Biochemistry 2020Quote: ... KBP was diluted to 0.3 mg/ml in KBP dilution buffer and 4 μl were applied to glow-discharged 1.2/1.3 AuFoil gold grids (Quantifoil®). For the third approach ...
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bioRxiv - Molecular Biology 2021Quote: Purified mtLSU or 55S mitoribosome samples (4 μL) were applied to freshly glow discharged R 3.5/1 holey carbon grids (Quantifoil) that were precoated with a 2-3 nm carbon layer using a Leica EM ACE600 coater ...
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bioRxiv - Microbiology 2022Quote: ... A 4□μl volume of SsoCsm complex was applied to holey carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh) covered by a graphene oxide layer (27) ...
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bioRxiv - Biophysics 2024Quote: ... For plunge freezing the cells were pipetted (4 μl) on EM grids (gold/200mesh/R2.2 either UltrAufoil or Quantifoil) in a humidified chamber (98% RH) ...
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bioRxiv - Cell Biology 2024Quote: ... 3.5 µl of 4 mg/mL proteasomal complexes was applied to glow-discharged R0.6/1 UltrAuFoil 300 mesh grids (Quantifoil) and frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... For plunge freezing the cells were pipetted (4 µl) on EM grids (gold/200mesh/R2.2 either UltrAufoil or Quantifoil) in a humidified chamber (98% RH) ...
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bioRxiv - Molecular Biology 2021Quote: ... and applied onto freshly glow-discharged 200 square mesh Ultrafoil R2/2 gold grids (Quantifoil). The samples were plunge-frozen with a FEI Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Freshly glow-discharged finder gold quantifoil grids (200 mesh R2/2 – Quantifoil MicroTools GmbH, Germany) were sterilized in 70% EtOH and placed in a glass-bottomed MatTek dish (MatTek Corporation ...
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bioRxiv - Microbiology 2021Quote: ... were applied to freshly washed and glow-discharged R2/2 holey carbon copper grids (Quantifoil). The grids were manually back blotted for 2 to 3s with filter paper ...
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bioRxiv - Biochemistry 2022Quote: ... Before spotting onto R2/1 grids with an extra 2 nm carbon layer (Quantifoil, Inc), the complex was diluted to a final concentration of ∼100 nM and a 5-fold excess of Met-tRNAiMet ...
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bioRxiv - Biochemistry 2021Quote: ... Cryo-EM grids were prepared using glow discharged R2/2 Cu 200 mesh grids (Quantifoil) pre-floated onto custom-made 2.9 nm continuous carbon film ...
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bioRxiv - Neuroscience 2022Quote: Holey copper or molybdenum EM grids (Quantifoil R 2/1 200 mesh; Quantifoil, Jena, Germany) were glow discharged (Harrick Plasma cleaner PDC-3XG ...
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bioRxiv - Molecular Biology 2023Quote: ... 3.5 μL) were applied to precoated (2 nm) R1.2/1.3 carbon-supported copper grids (Quantifoil), blotted for 4-5 s at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... 3.5 µL were applied to freshly glow-discharged R 2/1 holey carbon grids (Quantifoil) at 4 °C and 95% humidity ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were seeded onto glow discharged cryo-EM grids (Quantifoil 2/1, 200 mesh, Au) overnight ...
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bioRxiv - Immunology 2022Quote: ... were incubated for 3 min at room temperature and then loaded onto the glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3). The grids were washed using the buffer containing 20 mM HEPES ...
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bioRxiv - Biophysics 2021Quote: ... 3 μl of samples were applied onto a glow-discharged gold grids covered with holey carbon film (Quantifoil, 300 mesh 1.2/1.3) and blotted using a Vitrobot Mark IV (FEI Company ...
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bioRxiv - Biochemistry 2022Quote: A 3 µL of the protein solutions was applied onto the glow-discharged holey carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh). The grids were plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... 3.5 uL of sample was applied at a concentration of 3-5 mg/mL to glow-discharged holey carbon grids (Quantifoil R1.2/1.3). The grids were blotted using an FEI Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: The purified tirzepatide–GLP-1R–Gs–Nb35 complex (3 μL at about 20 mg/mL) was applied to a glow-discharged holey carbon grid (Quantifoil R1.2/1.3) and blotted subsequently ...
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bioRxiv - Microbiology 2020Quote: ... 3.5 μl of the freshly prepared mixture of the soluble S trimer and monomeric ACE2 (1:3 molar ratio) at ~1 mg/ml was applied to a 1.2/1.3 Quantifoil grid (Quantifoil Micro Tools GmbH, Germany), which had been glow discharged with a PELCO easiGlow™ Glow Discharge Cleaning system (Ted Pella ...
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bioRxiv - Molecular Biology 2023Quote: 3 µl of freshly purified NbE-µOR-Fab module complex were applied onto holey gold grids (Quantifoil UltrAuFoil R1.2/1.3, 300 mesh), front blotted for 3-4 seconds with 1 mm additional movement (95% humidity at 15°C ...
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bioRxiv - Biophysics 2023Quote: ... 3 μl of medium containing one isolated muscle fibre was placed on the glow-discharged grid (Quantifoil R2/1 Au 200 mesh). After incubation for 60 seconds at 37 °C ...
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bioRxiv - Biophysics 2021Quote: ... Log phase cultures were centrifuged at 2000 g for 2 minutes to concentrate the cells 10 times and 4 μl was directly applied to a glow-discharged holey carbon R2/1 Cu 200 mesh grid (Quantifoil). Plunge-freezing was performed using a VitroBot Mark 4 (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: 3.5 μl of protein samples at 4-6 mg/ml were applied to glow-discharged QF R1.2/1.3 300 mesh gold grids (Quantifoil, Großlöbichau, Germany). Grids were blotted for 3 s at 4°C and 100% humidity and plunge-frozen into liquid ethane using FEI Mark IV Vitrobot (FEI ...
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bioRxiv - Plant Biology 2022Quote: ... then 4 µl of the sample was applied to glow-discharged holey carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh). The grid was blotted for 4s and then plunged into liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Cell Biology 2024Quote: ... 4 μl of the cell suspension was applied onto a glow-discharged holey SiO2 grid (R1/4, Au, 200 mesh, Quantifoil) mounted on in the plunger chamber ...
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bioRxiv - Microbiology 2023Quote: ... trehalose solution to mitigate crystalline ice formation and 4 μL of this mixture immediately applied to a R2/1 Cu 200 grid (Quantifoil), which had been glow-discharged for 1 min at 0.19 mbar and 20 mA in a PELCO easiGlow device shortly before use ...
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bioRxiv - Molecular Biology 2023Quote: 1.8 × 105 A549 or A549-IFITM3 cells were seeded on glow-discharged 200 mesh gold grids with a holey SiO2 film with R1/4 spacing (Quantifoil) placed in a 35 mm dish and cultured in 2 ml complete growth medium (DMEM-F12 ...
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bioRxiv - Biophysics 2023Quote: ... 4 µL fresh sample (0.48 mg/mL) was applied on glow-discharged Au holey carbon grids (Quantifoil R 0.6/1) and the following operation were the same as describe above for preparing the sample of gMCM8/9 complex.