Labshake search
Citations for Quantifoil Micro Tools :
351 - 375 of 375 citations for Cytosolic arginine sensor for mTORC1 subunit 2 CASTOR2 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2024Quote: A 3 μL of aliquot Msmeg MmpL3 ΔC NDs at a concentration of 0.6 mg/mL was applied to glow-discharged (Quantifoil Cu R2/2, 300 mesh) holey carbon grids for 30 sec and blotted for 2×5 sec at 4°C and 100% humidity ...
-
bioRxiv - Biochemistry 2024Quote: ... 3 µl were frozen in liquid ethane with the Vitrobot Mark IV (FEI company) on 2/1 Quantifoil Multi A holey carbon supported grids (Quantifoil, Multi A, 400 mesh). Freezing was carried out with Whatman #1 filter papers at 4°C with a blot force of 0 ...
-
bioRxiv - Biochemistry 2021Quote: ... 2.5-μL aliquots of purified Neo1 at a concentration of about 1 mg/mL were placed on glow-discharged holey carbon grids (Quantifoil Au R2/2, 300 mesh) and were flash-frozen in liquid ethane using a FEI Vitrobot Mark IV ...
-
bioRxiv - Microbiology 2020Quote: ... A small volume (4 µL) of cells was then transferred onto freshly glow‐discharged copper R2/2 200 Quantifoil holey carbon grids (Quantifoil Micro Tools GmbH, Jena, Germany). Grids were then blotted and plunge‐frozen in a liquid ethane/propane mixture 31 using an FEI Vitrobot Mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2022Quote: ... Samples were prepared by depositing 3 μl of the crystalline slurry onto a glow-discharged EM grid (Quantifoil, Cu 200 mesh, R2/2 holey carbon). Excess liquid was blotted away and the sample was vitrified using a Leica GP2 vitrification robot ...
-
bioRxiv - Molecular Biology 2022Quote: RNA granule preparations were diluted with RNA Granule buffer to an optical density A260 = 2 U/mL aliquots of the dilution deposited on glow-discharged Quantifoil grids (Quantifoil 1.2/1.3, 300 mesh copper) pre-coated with a continuous layer of 3 nm carbon ...
-
bioRxiv - Molecular Biology 2022Quote: 3 μL of ~120 nM mitoribosome was applied onto a glow-discharged (20 mA for 30 sec) holey-carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ~3 nm thickness ...
-
bioRxiv - Biophysics 2020Quote: ... 2 μL of a 1 in 6 dilution of the batch crystallisation was applied to the carbon side of glow discharged R2/2 Quantifoil™ grids (Quantifoil Micro Tools, Jena, Germany). Excess liquid was removed by blotting for 4-6 s with a Vitrobot (Thermo Fisher ...
-
bioRxiv - Microbiology 2020Quote: ... 3.5 μl of that mixture was transferred to a 3 mm freshly glow-discharged Quantifoil R2/2 Cu 300 mesh holey carbon grid (Quantifoil Micro Tools GmbH, Jena, Germany). Excess liquid was blotted away using a Leica EM GP plunge-freezer at room temperature and 80% humidity (blot time 2-3 sec ...
-
bioRxiv - Molecular Biology 2022Quote: ... The pellet was then resuspended in warm EM buffer and 3 μL of this sample was applied to a carbon on gold grid (Quantifoil R2/2 Au 200 mesh), recently glow-discharged for 1 min at 30 mA ...
-
bioRxiv - Biochemistry 2022Quote: ... The endogenous samples were applied onto grids containing an additional thin layer of continuous carbon support (Quantifoil R1.2/1.3 Cu 200 mesh + 2 nm C) and incubated for 30 sec before blotting and freezing ...
-
bioRxiv - Neuroscience 2024Quote: ... Supernatant after centrifugation mixed with 10-nm gold beads solution (Aurion, cat # 25486) at 1:1 ratio was added to the mounted grid (Quantifoil R2/2 Cu 200 EM). No other chemicals or stimulation were applied prior to plunge freezing ...
-
bioRxiv - Biochemistry 2024Quote: ... or calpain-3-titin/I81-I83 (2mg/mL) from Superose 6 increase 10/300GL column chromatography was loaded onto glow-discharged holey carbon grids (Quantifoil 1.2/1.3 + 2 nm Carbon). The grids were blotted with filter paper for 2.5 secs at 22 °C and then immediately plunged into liquid ethane cooled by liquid nitrogen using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
-
bioRxiv - Biochemistry 2023Quote: 3 μL of ∼120 nM mitoribosome was applied onto a glow-discharged (20 mA for 30 sec) holey- carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ∼3 nm thickness ...
-
bioRxiv - Biophysics 2023Quote: ... 4 μl of the extracellular parasites were pipetted onto a glow-discharged (30 seconds at −30 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools GmbH, Jena, Germany). Samples were back-blotted with a Whatman filter paper (grade 1 ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... a volume of 6-8 μL of each sample was applied onto a gold grid covered by a holey gold film (UltrAuFoil 2/1, Quantifoil Micro Tools GmbH, Jena, Germany). Excess of liquid was blotted automatically between two strips of filter paper or only from the backside of the Grid ...
-
bioRxiv - Biochemistry 2023Quote: ... 3 μL of ∼120 nM mitoribosome was applied onto a glow-discharged (20 mA for 30 sec) holey-carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ∼3 nm thickness ...
-
bioRxiv - Microbiology 2024Quote: ... The co-culture-colloidal gold bead mixture was added to glow-discharged copper R2/2 Quantifoil holey carbon grids (Quantifoil Micro Tools GmbH, Jena, Germany). Grids were blotted (under 100% humidity conditions ...
-
bioRxiv - Biochemistry 2022Quote: The samples were frozen on copper or gold grids with holey carbon film coated with a 2 nm continuous carbon film (Quantifoil R1.2/1.3 ultrathin carbon, 300 mesh) freshly glow discharged for 20 s at 15 mA (PELCO easiGlow) ...
-
bioRxiv - Biochemistry 2023Quote: ... A sample of 3.0 μM of FH2 and 10.6 μM of actin monomers was applied to holey grids without a carbon film (Quantifoil Au 2/1 300 mesh from EMS). After diluting 4-fold ...
-
bioRxiv - Neuroscience 2023Quote: ... 3.5 μL of CopiaGag capsid protein sample was applied to a 300-mesh copper grid with continuous 2 nm carbon film (Electron Microscopy Sciences, QUANTIFOIL® C2-C15nCu30-50) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
-
bioRxiv - Molecular Biology 2024Quote: ... about 0.9 mg/ml for DSS1–INTAC–PEC complex) was applied onto a glow-discharged holey carbon grid (Quantifoil Au, R1.2/1.3 or R2/2, 300 mesh). After blotting for 3 to 6 s ...
-
bioRxiv - Biochemistry 2023Quote: ... A 3 µL drop was applied to a glow-discharged (PELCO easiGlow, 15 mA, 2 min) holey carbon grid (Quantifoil R1.2/1.3 on gold 200 mesh support), blotted for 3-4 seconds with Whatman Grade 595 filter paper (GE Healthcare) ...
-
bioRxiv - Biochemistry 2022Quote: Freshly prepared ribosome–Sec61–KZR-8445 complex at 300–500 nM concentration was applied on glow discharged holey-carbon grids (Quantifoil, R1.2/1.3 with a 2-nm carbon film) and flash frozen with an automated plunge freezer (Leica microsystems) ...
-
bioRxiv - Plant Biology 2024Quote: 4 μL AtEDS1-AtPAD4-AtADR1 complex purified from Nb leaves was applied to glow-discharged Quantifoil holey carbon grids (Quantifoil Cu R1.2/1.3+2 nm C, 300 mesh). The protein was concentrated to 0.9 mg/mL prior to freezing ...