Labshake search
Citations for Quantifoil Micro Tools :
351 - 400 of 470 citations for 2 2 4 Dibromophenoxy methyl tetrahydro 2H pyran since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 4 µL of the mixture were pipetted onto a copper EM grid with holey carbon film (R2/2, 200 mesh, Quantifoil Micro Tools GmbH, Q43486) that had been freshly glow-discharged for 30 s at -30 mAmp ...
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bioRxiv - Biophysics 2024Quote: A 3 μL of aliquot Msmeg MmpL3 ΔC NDs at a concentration of 0.6 mg/mL was applied to glow-discharged (Quantifoil Cu R2/2, 300 mesh) holey carbon grids for 30 sec and blotted for 2×5 sec at 4°C and 100% humidity ...
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bioRxiv - Biochemistry 2024Quote: ... 3 µl were frozen in liquid ethane with the Vitrobot Mark IV (FEI company) on 2/1 Quantifoil Multi A holey carbon supported grids (Quantifoil, Multi A, 400 mesh). Freezing was carried out with Whatman #1 filter papers at 4°C with a blot force of 0 ...
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bioRxiv - Biochemistry 2021Quote: ... 2.5-μL aliquots of purified Neo1 at a concentration of about 1 mg/mL were placed on glow-discharged holey carbon grids (Quantifoil Au R2/2, 300 mesh) and were flash-frozen in liquid ethane using a FEI Vitrobot Mark IV ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were prepared by depositing 3 μl of the crystalline slurry onto a glow-discharged EM grid (Quantifoil, Cu 200 mesh, R2/2 holey carbon). Excess liquid was blotted away and the sample was vitrified using a Leica GP2 vitrification robot ...
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bioRxiv - Molecular Biology 2022Quote: RNA granule preparations were diluted with RNA Granule buffer to an optical density A260 = 2 U/mL aliquots of the dilution deposited on glow-discharged Quantifoil grids (Quantifoil 1.2/1.3, 300 mesh copper) pre-coated with a continuous layer of 3 nm carbon ...
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bioRxiv - Molecular Biology 2022Quote: 3 μL of ~120 nM mitoribosome was applied onto a glow-discharged (20 mA for 30 sec) holey-carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ~3 nm thickness ...
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bioRxiv - Biophysics 2020Quote: ... 2 μL of a 1 in 6 dilution of the batch crystallisation was applied to the carbon side of glow discharged R2/2 Quantifoil™ grids (Quantifoil Micro Tools, Jena, Germany). Excess liquid was removed by blotting for 4-6 s with a Vitrobot (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... 3.5 μl of that mixture was transferred to a 3 mm freshly glow-discharged Quantifoil R2/2 Cu 300 mesh holey carbon grid (Quantifoil Micro Tools GmbH, Jena, Germany). Excess liquid was blotted away using a Leica EM GP plunge-freezer at room temperature and 80% humidity (blot time 2-3 sec ...
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bioRxiv - Molecular Biology 2022Quote: ... The pellet was then resuspended in warm EM buffer and 3 μL of this sample was applied to a carbon on gold grid (Quantifoil R2/2 Au 200 mesh), recently glow-discharged for 1 min at 30 mA ...
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bioRxiv - Biochemistry 2022Quote: ... The endogenous samples were applied onto grids containing an additional thin layer of continuous carbon support (Quantifoil R1.2/1.3 Cu 200 mesh + 2 nm C) and incubated for 30 sec before blotting and freezing ...
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bioRxiv - Neuroscience 2024Quote: ... Supernatant after centrifugation mixed with 10-nm gold beads solution (Aurion, cat # 25486) at 1:1 ratio was added to the mounted grid (Quantifoil R2/2 Cu 200 EM). No other chemicals or stimulation were applied prior to plunge freezing ...
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bioRxiv - Biochemistry 2024Quote: ... or calpain-3-titin/I81-I83 (2mg/mL) from Superose 6 increase 10/300GL column chromatography was loaded onto glow-discharged holey carbon grids (Quantifoil 1.2/1.3 + 2 nm Carbon). The grids were blotted with filter paper for 2.5 secs at 22 °C and then immediately plunged into liquid ethane cooled by liquid nitrogen using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: 3 μL of ∼120 nM mitoribosome was applied onto a glow-discharged (20 mA for 30 sec) holey- carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ∼3 nm thickness ...
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bioRxiv - Biophysics 2023Quote: ... 4 μl of the extracellular parasites were pipetted onto a glow-discharged (30 seconds at −30 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools GmbH, Jena, Germany). Samples were back-blotted with a Whatman filter paper (grade 1 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... a volume of 6-8 μL of each sample was applied onto a gold grid covered by a holey gold film (UltrAuFoil 2/1, Quantifoil Micro Tools GmbH, Jena, Germany). Excess of liquid was blotted automatically between two strips of filter paper or only from the backside of the Grid ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μL of ∼120 nM mitoribosome was applied onto a glow-discharged (20 mA for 30 sec) holey-carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ∼3 nm thickness ...
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bioRxiv - Microbiology 2024Quote: ... The co-culture-colloidal gold bead mixture was added to glow-discharged copper R2/2 Quantifoil holey carbon grids (Quantifoil Micro Tools GmbH, Jena, Germany). Grids were blotted (under 100% humidity conditions ...
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bioRxiv - Biochemistry 2022Quote: The samples were frozen on copper or gold grids with holey carbon film coated with a 2 nm continuous carbon film (Quantifoil R1.2/1.3 ultrathin carbon, 300 mesh) freshly glow discharged for 20 s at 15 mA (PELCO easiGlow) ...
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bioRxiv - Biochemistry 2023Quote: ... A sample of 3.0 μM of FH2 and 10.6 μM of actin monomers was applied to holey grids without a carbon film (Quantifoil Au 2/1 300 mesh from EMS). After diluting 4-fold ...
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bioRxiv - Neuroscience 2023Quote: ... 3.5 μL of CopiaGag capsid protein sample was applied to a 300-mesh copper grid with continuous 2 nm carbon film (Electron Microscopy Sciences, QUANTIFOIL® C2-C15nCu30-50) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
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bioRxiv - Molecular Biology 2024Quote: ... about 0.9 mg/ml for DSS1–INTAC–PEC complex) was applied onto a glow-discharged holey carbon grid (Quantifoil Au, R1.2/1.3 or R2/2, 300 mesh). After blotting for 3 to 6 s ...
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bioRxiv - Biochemistry 2023Quote: ... A 3 µL drop was applied to a glow-discharged (PELCO easiGlow, 15 mA, 2 min) holey carbon grid (Quantifoil R1.2/1.3 on gold 200 mesh support), blotted for 3-4 seconds with Whatman Grade 595 filter paper (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: Freshly prepared ribosome–Sec61–KZR-8445 complex at 300–500 nM concentration was applied on glow discharged holey-carbon grids (Quantifoil, R1.2/1.3 with a 2-nm carbon film) and flash frozen with an automated plunge freezer (Leica microsystems) ...
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bioRxiv - Cell Biology 2020Quote: ... or holey SiO2 R1/4 TEM grids (Quantifoil) to allow for more robust correlation and cells were plunge frozen on a Vitrobot Mark IV (FEI ...
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bioRxiv - Cell Biology 2022Quote: ... Grids (200 Mesh Cu SiO2 R1/4, Quantifoil) were plasma cleaned for 30 s before plunging ...
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bioRxiv - Plant Biology 2020Quote: ... 4 μL of cell culture was blotted onto R 1/4 SiO2-coated 200-mesh gold EM grids (Quantifoil Micro Tools) and plunge frozen in a liquid ethane/propane mixture ...
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bioRxiv - Biophysics 2024Quote: ... We glow-discharge-treated 200 mesh R1/4 or R2/4 Quantifoil holey carbon gold grids (Quantifoil®, Grossloebichau/Jena Germany) in a Pelco easiGlow (Ted Pella ...
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bioRxiv - Cell Biology 2024Quote: ... and then 4 μL of the sample was applied to the glow-discharged R1/4 Carbon 200-mesh gold EM grid (Quantifoil Micro Tools). The EM grid was incubated in the chamber of Vitrobot (Vitrobot Mark 4 ...
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bioRxiv - Biophysics 2021Quote: Gold EM grids with R1/4 holey SiO2 film (Quantifoil) were glow-discharged in a EasiGlow (Pelco ...
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bioRxiv - Biophysics 2023Quote: 200 mesh Au grids with R1/4 SiO2 foil (Quantifoil) were glow-discharged for 90 sec at 0.38 mBar and 15 mA using a Pelco easiGlow device ...
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bioRxiv - Neuroscience 2022Quote: ... Gold Quantifoil grids (R1/4, Au 200 mesh, Quantifoil Micro Tools) were additionally coated with a 20-25 nm carbon layer (MED 020 ...
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bioRxiv - Cell Biology 2024Quote: ... with holey R 1/4 carbon film (Quantifoil Micro Tools GmbH) were glow-discharged for 30 seconds at 5 mA using an EasiGlow system (Pelco ...
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bioRxiv - Microbiology 2023Quote: ... Gold grids with SiO2 film (Quantifoil, Au 200 mesh, R 1/4) were glow- discharged and coated with 20 µg/ml fibronectin solution for 30 min ...
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bioRxiv - Biophysics 2021Quote: ... with 12-nm-thick holey R1/4 and R1.2/20 SiO2 film (Quantifoil) were used for the experiments ...
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bioRxiv - Cell Biology 2021Quote: ... Gold Quantifoil grids (R1/4, Au 200 mesh grid, SiO2, Quantifoil Micro Tools) were glow-discharged and UV irradiated for 30 min for sterilization before being immersed in cell culture medium in 35-mm IBIDI μ-Dish ...
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bioRxiv - Synthetic Biology 2023Quote: 4 µl of sample was adsorbed onto holey carbon-coated grid (Quantifoil, Germany) that was glow-discharged ...
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bioRxiv - Neuroscience 2024Quote: ... gold mesh grids with holey R1/4 SiO2 film (Quantifoil Micro Tools GmbH) were glow discharged for 60s at 15 mA using an EasiGlow system ...
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bioRxiv - Cell Biology 2024Quote: EM grids (Quantifoil R 1/4 Au 200 mesh, Quantifoil Micro Tools GmbH) were glow discharged with an Edwards S150B glow discharger at 30mA for 45sec on both sides of the grid ...
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bioRxiv - Cell Biology 2024Quote: 4 µl of yeast cells were pipetted on Cu R2/1 grids (Quantifoil), blotted manually from the backside of the grid for ∼4 s and plunge frozen in liquid ethane using a manual plunger ...
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bioRxiv - Biophysics 2020Quote: ... 4 µl sample was applied to each freshly glow-discharged grid (Quantifoil, R1.2/1.3). The grids were then plunge-frozen in liquid ethane ...
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bioRxiv - Microbiology 2021Quote: ... 4-7 µl of cells were deposited on R2/1 Cu 200 grids (Quantifoil) that had been glow-discharged for 1 min at 0.19 mbar and 20 mA in a PELCO easiGlow device shortly before use ...
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bioRxiv - Biophysics 2021Quote: ... 4 μl of cell suspension was applied to holey carbon R2/1 Cu grids (Quantifoil) and plunge-frozen on a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... 4 µl of this sample was then applied to plasma-cleaned holey carbon grids (Quantifoil) and plunge frozen with an FEI vitrobot mark IV at 4°C and 100% humidity.
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bioRxiv - Cell Biology 2024Quote: Quantifoil R2/1 or SiO2 R1/4 gold grids (both Quantifoil Mirco Tools GmbH, Germany) were briefly washed in chloroform and placed on drops of poly-D-lysine (100 µg/ml) ...
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bioRxiv - Biochemistry 2023Quote: ... 4 μl of sample was applied to glow discharged Quantifoil 300 mesh 1.2/1.3 (Quantifoil) grids at 4 °C and 90-100% humidity ...
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bioRxiv - Biophysics 2024Quote: 4 μL of sample were applied onto an UltrAuFoil grid (Quantifoil R1.2/1.3, 300 mesh), which was rendered hydrophilic by a 90 s treatment in an ELMO glow discharge system operating at 3 mA and in a partial vacuum of 3.6 × 10-1 mbar ...
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bioRxiv - Biochemistry 2020Quote: ... 4 µL of sample was applied to Quantifoil Cu R 1.2/1.3 grids (Quantifoil Microtools GmbH) which has undergone a plasma discharge and were vitrified in liquid ethane using a Vitrobot MkIV plunge freezer (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... Aliquots of 4 µl were applied to glow-discharged 300 mesh R1.2/1.3 Au grids (Quantifoil) using a Vitrobot Mark VI (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... 4 μL of Kapβ2•HNRNPH2 was applied to a 300 mesh copper grid (Quantifoil R1.2/1.3) that was glow-discharged using a PELCO easiGlow glow discharge apparatus at 30 mA/30 s on top of a metal grid holder (Ted Pella) ...