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Citations for Quantifoil Micro Tools :
301 - 350 of 553 citations for 8 CYANO IMIDAZO 1 2 A PYRIDINE 2 CARBOXYLIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Mycobacteria form viable cell wall-deficient cells that are undetectable by conventional diagnosticsbioRxiv - Microbiology 2022Quote: ... of which 3-4 µL was applied on glow-discharged 200 mesh copper grids with an extra thick R2/2 carbon film (Quantifoil Micro Tools). Vitrification was performed using an automated Leica EM GP plunge-freezer ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μl of the resulting mixture was spotted onto glow-discharged copper EM grids (Quantifoil 300 Au mesh, R2/2 carbon film (Quantifoil Micro Tools). The grids were then blotted for 2 or 4 seconds (blot force −10 ...
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bioRxiv - Cell Biology 2022Quote: ... 3 µl of the microtubule sample was deposited at the surface of a glow-discharged holey carbon grid (Quantifoil R2/2, Cu200) in the temperature (35 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were applied to freshly glow-discharged (Edwards S150B, 45 s, 30−50 mA, 1.2 kV, 10−2 mbar) copper holey carbon grids (Quantifoil, Cu-R1.2/1.3) under 100% humidity at 4°C.
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bioRxiv - Biochemistry 2023Quote: 4.5 µL of freshly mixed Nup98 condensate and capsid mixture with protein A-gold (10 nm) were applied onto a glow-discharged Quantifoil R2/2 copper grid (Quantifoil Micro Tools). The grid was blotted in the front for 2.5 s at 15 °C with 90% relative humidity and then plunged into liquid ethane using a Lecia EM GP device (Lecia Microsystem) ...
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bioRxiv - Cell Biology 2024Quote: ... An aliquot of a 5 μl sample was applied to a glow-discharged holey carbon grid (Quantifoil R2/2, Au, 400 mesh). After waiting for 60 s ...
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bioRxiv - Biophysics 2022Quote: Purified wt or mutant D263A or F55A MVMp capsid samples (5 μl) were applied onto R2/2 300 mesh copper grids (Quantifoil Micro Tools, Germany) and vitrified using a Leica EM CPC cryofixation unit ...
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bioRxiv - Biochemistry 2020Quote: ... 3 μl aliquots of purified mitoribsomes was incubated for 30 s on glow-discharged holey carbon grids (Quantifoil R2/2, 300 mesh, copper) pre-coated with a home-made continuous carbon film with thickness of ~ 27 Å ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μL of urease samples at 1.3 mg/mL with 1 mM BME or with 10 mM SHA were applied to freshly glow-discharged Quantifoil R2/2 holey carbon grids (Quantifoil Micro Tools, Germany). The grids were blotted for 4 seconds ...
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bioRxiv - Biochemistry 2022Quote: ... and an aliquot of 2 μl of this sample was applied onto a plasma cleaned holey carbon grid (Quantifoil, R1.2/1.3, 200 mesh). The grid was blotted with Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: A 5 µl cell suspension containing 10 nm colloidal gold particles were deposited on the copper EM grids with holey carbon supported film (Quantifoil, 200mesh, R1/2). The EM grid were blotted with filter paper (Waterman ...
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bioRxiv - Cell Biology 2022Quote: ... A 3 µl volume of the Kif5B suspension was first deposited at the surface of a glow-discharged holey carbon grid (Quantifoil R2/2, Cu200) in the temperature (23 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Grids were prepared by applying 3.5 μLs of the Z7757 complex or 2 μLs of the Lisuride complex to glow discharged UltrAuFoil holey gold grids (Quantifoil, Au300-R1.2/1.3). The girds containing Z7757 were then vitrified by plunge freezing into a 60/40 mixure of liquid ethane/propane using a Vitrobot mark IV (FEI ...
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bioRxiv - Cell Biology 2020Quote: 4 µl of the cultured yeast cells (OD600 of 0.4) were added to a glow-discharged (30 seconds at -30 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools GmbH, Jena, Germany), then the grid was rapidly plunge frozen in liquid ethane using a homemade plunge freezer and stored in liquid nitrogen until used ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 μl of protein sample (about 0.73 mg/ml) was applied onto a glow-discharged holey carbon grid (Quantifoil Au, R2/2, 300 mesh). After blotting for 3 s ...
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bioRxiv - Biochemistry 2020Quote: ... 2.5 μL aliquots of purified Dnf1p–Lem3p or Dnf2p–Lem3p at a concentration of about 1.5 mg/mL were placed on glow-discharged holey carbon grids (Quantifoil Au R2/2, 300 mesh) and were flash-frozen in liquid ethane using a FEI Vitrobot Mark IV ...
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bioRxiv - Cell Biology 2022Quote: ... 4 µL of the mixture were pipetted onto a copper EM grid with holey carbon film (R2/2, 200 mesh, Quantifoil Micro Tools GmbH, Q43486) that had been freshly glow-discharged for 30 s at -30 mAmp ...
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bioRxiv - Microbiology 2020Quote: ... A small volume (4 µL) of cells was then transferred onto freshly glow‐discharged copper R2/2 200 Quantifoil holey carbon grids (Quantifoil Micro Tools GmbH, Jena, Germany). Grids were then blotted and plunge‐frozen in a liquid ethane/propane mixture 31 using an FEI Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were prepared by depositing 3 μl of the crystalline slurry onto a glow-discharged EM grid (Quantifoil, Cu 200 mesh, R2/2 holey carbon). Excess liquid was blotted away and the sample was vitrified using a Leica GP2 vitrification robot ...
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bioRxiv - Molecular Biology 2022Quote: RNA granule preparations were diluted with RNA Granule buffer to an optical density A260 = 2 U/mL aliquots of the dilution deposited on glow-discharged Quantifoil grids (Quantifoil 1.2/1.3, 300 mesh copper) pre-coated with a continuous layer of 3 nm carbon ...
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bioRxiv - Molecular Biology 2022Quote: 3 μL of ~120 nM mitoribosome was applied onto a glow-discharged (20 mA for 30 sec) holey-carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ~3 nm thickness ...
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bioRxiv - Cell Biology 2019Quote: ... 4 µl of the solution was applied to a glow-discharged (30 s at −35 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools GmbH, Jena, Germany). After removing excess liquid by blotting the grid from the back side with Whatman filter paper No ...
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bioRxiv - Microbiology 2019Quote: ... Four μl of this mixture was applied onto freshly glow-discharged copper R2/2 of th0 Quantifoil holey carbon grids (Quantifoil Micro Tools GmbH, Jena, Germany). Using an FEI Vitrobot Mark IV ...
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bioRxiv - Biophysics 2020Quote: ... 2 μL of a 1 in 6 dilution of the batch crystallisation was applied to the carbon side of glow discharged R2/2 Quantifoil™ grids (Quantifoil Micro Tools, Jena, Germany). Excess liquid was removed by blotting for 4-6 s with a Vitrobot (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... 3.5 μl of that mixture was transferred to a 3 mm freshly glow-discharged Quantifoil R2/2 Cu 300 mesh holey carbon grid (Quantifoil Micro Tools GmbH, Jena, Germany). Excess liquid was blotted away using a Leica EM GP plunge-freezer at room temperature and 80% humidity (blot time 2-3 sec ...
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bioRxiv - Molecular Biology 2022Quote: ... The pellet was then resuspended in warm EM buffer and 3 μL of this sample was applied to a carbon on gold grid (Quantifoil R2/2 Au 200 mesh), recently glow-discharged for 1 min at 30 mA ...
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bioRxiv - Biochemistry 2022Quote: ... The endogenous samples were applied onto grids containing an additional thin layer of continuous carbon support (Quantifoil R1.2/1.3 Cu 200 mesh + 2 nm C) and incubated for 30 sec before blotting and freezing ...
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bioRxiv - Biochemistry 2023Quote: 3 μL of ∼120 nM mitoribosome was applied onto a glow-discharged (20 mA for 30 sec) holey- carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ∼3 nm thickness ...
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bioRxiv - Biophysics 2023Quote: ... 4 μl of the extracellular parasites were pipetted onto a glow-discharged (30 seconds at −30 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools GmbH, Jena, Germany). Samples were back-blotted with a Whatman filter paper (grade 1 ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μL of ∼120 nM mitoribosome was applied onto a glow-discharged (20 mA for 30 sec) holey-carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ∼3 nm thickness ...
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bioRxiv - Biochemistry 2024Quote: ... or calpain-3-titin/I81-I83 (2mg/mL) from Superose 6 increase 10/300GL column chromatography was loaded onto glow-discharged holey carbon grids (Quantifoil 1.2/1.3 + 2 nm Carbon). The grids were blotted with filter paper for 2.5 secs at 22 °C and then immediately plunged into liquid ethane cooled by liquid nitrogen using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2019Quote: ... Approximately 5 µL of sample (diluted if necessary) was placed on a copper grid (Quantifoil® S7/2 Cu 400 mesh, holey carbon films, Quantifoil Micro Tools GmbH, Jena, Germany) and any excess liquid was absorbed by a filter paper ...
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bioRxiv - Biochemistry 2022Quote: The samples were frozen on copper or gold grids with holey carbon film coated with a 2 nm continuous carbon film (Quantifoil R1.2/1.3 ultrathin carbon, 300 mesh) freshly glow discharged for 20 s at 15 mA (PELCO easiGlow) ...
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bioRxiv - Neuroscience 2023Quote: ... 3.5 μL of CopiaGag capsid protein sample was applied to a 300-mesh copper grid with continuous 2 nm carbon film (Electron Microscopy Sciences, QUANTIFOIL® C2-C15nCu30-50) at 10 °C with 90% humidity in a Vitrobot Mark IV (FEI) ...
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bioRxiv - Biochemistry 2023Quote: ... A 3 µL drop was applied to a glow-discharged (PELCO easiGlow, 15 mA, 2 min) holey carbon grid (Quantifoil R1.2/1.3 on gold 200 mesh support), blotted for 3-4 seconds with Whatman Grade 595 filter paper (GE Healthcare) ...
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bioRxiv - Molecular Biology 2024Quote: ... about 0.9 mg/ml for DSS1–INTAC–PEC complex) was applied onto a glow-discharged holey carbon grid (Quantifoil Au, R1.2/1.3 or R2/2, 300 mesh). After blotting for 3 to 6 s ...
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bioRxiv - Biochemistry 2019Quote: 2.5 µl of freshly purified protein at a concentration of 3.3 mg/ml when solubilized in DDMand at about 2 mg/ml when reconstituted in nanodiscs were applied on holey-carbon cryo-EM grids (Quantifoil Au R1.2/1.3, 200, 300 and 400 mesh), which were prior glow-discharged at 5 mA for 30 s ...
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bioRxiv - Biochemistry 2022Quote: Freshly prepared ribosome–Sec61–KZR-8445 complex at 300–500 nM concentration was applied on glow discharged holey-carbon grids (Quantifoil, R1.2/1.3 with a 2-nm carbon film) and flash frozen with an automated plunge freezer (Leica microsystems) ...
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bioRxiv - Biochemistry 2020Quote: A droplet of 3 μl of purified GluD2 Δ870 at a concentration of ~0.8 mg/ml was applied onto a glow discharged 1.2/1.3 300 mesh ultra foil gold grid (Quantifoil). It was blotted for 3 seconds at a blot force of 0 using the FEI Vitrobot Mark IV at 14°C and 95 % humidity ...
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bioRxiv - Biophysics 2020Quote: ... 2.8 μl of sample was pipetted onto glow-discharged R1.2/1.3 200 mesh Au holey carbon grids (Quantifoil) and then plunge-frozen in a liquid ethane/propane mixture with a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... 3 μL of the purified complex (8 mg/ml) was deposited onto a glow-discharged holey grid (Quantifoil Au 300 mesh R1.2/1.3 ...
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bioRxiv - Biophysics 2022Quote: Eleven cryoEM grids were prepared by two people by adding 3 μL of mouse apoferritin (mApof, from Dr. Kikkawa’s lab) solution (8 mg/mL) to UltrAuFoil 1.2/1.3 grids (Quantifoil, Jena, Germany) immediately after plasma cleaning (Gatan Solarus II plasma cleaner ...
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bioRxiv - Immunology 2023Quote: ... 3 μL of the purified complex (8 mg/ml) was deposited onto a glow-discharged holey grid (Quantifoil Au 300 mesh R1.2/1.3, Quantifoil, Großlöbichau, Germany). It was then plunge-frozen in liquid ethane using the Vitrobot Mark IV (Thermo Fischer Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... with a surface-active additive (0.01% (w/v) glycyrrhizic acid) was added to a glow discharged (PELCO easiGlow) holey gold grid (Quantifoil UltrAuFoil grids (R0.6/1 ...
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bioRxiv - Biophysics 2021Quote: ... 3 μl of purified ghrelin-bound complex at 13 mg/ml and GHRP-6-bound ghrelin receptor complex at 8 mg/ml were applied individually onto a glow-discharged holey carbon grid (Quantifoil, Au300 R1.2/1.3) in a Vitrobot chamber (FEI Vitrobot Mark IV) ...
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bioRxiv - Molecular Biology 2022Quote: The purified complex samples (3 μL at 5-8 mg/mL) were applied to glow-discharged holey grids (Quantifoil R1.2/1.3, 300 mesh) and subsequently vitrified using a Vitrobot Mark IV (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... Sample volumes of 3.5 µl (8 OD260 per ml) were applied to grids (Quantifoil, Cu, 300 mesh, R3/3 with 3 nm carbon) which had been freshly glow-discharged using a GloQube (Quorum Technologies ...
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bioRxiv - Biochemistry 2024Quote: A volume of 3 µl purified protein complex at a concentration of 1mg/ml (JSR1-hGi) or 8 mg/ml (JSR1-jsGiq) was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3 Cu 200 mesh). The grids were blotted with a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... or AE1-Niflumic acid at 5 mg/ml were applied to glow-discharged holey carbon EM grids (Quantifoil 300 copper mesh, R1.2/1.3) in an EM-GP2 plunge freezer (Leica) ...
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bioRxiv - Bioengineering 2022Quote: ... holey carbon grids (Quantifoil R2/1 grid: Cu 300mesh, M2951C-1-300) were hydrophilized by a JEC-3000FC Auto Fine Coater (JEOL ...