Labshake search
Citations for Quantifoil Micro Tools :
301 - 350 of 414 citations for 5 2 Chloronicotinoyl 2 furoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 3 μL of the sample mixture was applied to a freshly glow-discharged copper R2/2 200 grid (Quantifoil Micro Tools), pre-blotted for 30 seconds ...
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bioRxiv - Biochemistry 2021Quote: ... Three microliters of the protein solution were applied on a glow-discharged holey carbon grids (200 mesh Quantifoil R2/2 molybdenum), which had been treated with H2 and O2 mixtures in a Solarus plasma cleaner (Gatan ...
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bioRxiv - Cell Biology 2022Quote: ... The IL-11Δ10/IL-11RαD1-D3/gp130D1-D3 complex (0.5 mg/mL) was blotted onto UltrAuFoil grids (R2/2, Quantifoil Micro Tools GmbH) that had been subjected to glow discharge (15 mA for 30 s) ...
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bioRxiv - Microbiology 2023Quote: ... sample were placed onto glow-discharged Quantifoil R2/1 Cu300 holey carbon grids (1 µm spacing, 2 µm holes, copper mesh) (Quantifoil, Germany), blotted for 3.5 s before flash-freezing in liquid ethane using a Vitrobot mark IV (FEI ...
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bioRxiv - Biophysics 2023Quote: ... 3 to 3.5 μl of complex was applied to a glow-discharged UltrAuFoil Holey Gold 200 mesh R2/2 grid (Quantifoil, Catalog # Q250AR2A) and incubated in a FEI Vitrobot IV chamber at 4°C and 95% humidity for 20 sec ...
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bioRxiv - Biochemistry 2024Quote: ... 3 µL of suspended cells in culture medium were applied to the front side of glow-discharged R2/2 carbon-coated copper grids (Quantifoil MicroTools), with additional 1 µL cells added to the back side ...
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bioRxiv - Cancer Biology 2024Quote: ... or 0.5 mg/ml of RanGTP•selinexor-XPO1•ASB8ΔN16•ELOB/C supplemented with 2-fold excess ASB8ΔN16-ELOB/C and selinexor) was applied to a 400-mesh copper grid (Quantifoil R1.2/1.3). For unliganded XPO1 ...
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bioRxiv - Biophysics 2021Quote: 3 μl of the human sperm sample were applied to glow discharged copper R2/2 200-mesh holey carbon grid (Quantifoil Micro Tools) and plunge frozen in liquid ethane using an automatic plunge freezer (Vitrobot ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 μL aliquots of the cell suspension were applied to glow-discharged R2/2 200 mesh copper Quantifoil grids (Quantifoil Micro Tools), the sample was pre-blotted for 30 seconds ...
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bioRxiv - Molecular Biology 2021Quote: ... After another 3 min at 37 °C the termination complexes were placed on ice for 15 min and subsequently applied to Quantifoil R2/2 holey carbon grids (Quantifoil Micro Tools) either coated with a thin continuous carbon film or with graphene oxide flakes (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... 4 µl of the solution was applied to a glow-discharged (30s at 35 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools). After removing excess liquid by blotting the grid from the back side with Whatman filter paper for 2 s ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were applied to freshly glow-discharged (Edwards S150B, 45 sec, 30-50 mA, 1,2kV, 10-2 mbar) copper holey carbon grids (Quantifoil, Cu-R1.2/1.3) under 100% humidity ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μl of the mixture was deposited on glow discharged R2/2 Copper 300 mesh holey carbon-coated support grids (Quantifoil, Jena, Germany) along with 1 μl of HEPES pH 10 and 1 μl of hydrogen peroxide 0.9 % ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: A 4 μL solution of co-isolated small particles were applied to glow-discharged copper grids (Quantifoil R2/2, Quantifoil Micro Tools), blotting for 3-seconds at 4 °C with 90% humidity and plunging into liquid ethane using a Lecia EM GP device (Leica Microsystems ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: A 4 μL solution of co-isolated small particles were applied to glow-discharged copper grids (Quantifoil R2/2, Quantifoil Micro Tools), blotting for 3-seconds at 4 °C with 90% humidity and plunging into liquid ethane using a Lecia EM GP device (Leica Microsystems ...
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bioRxiv - Neuroscience 2021Quote: ... 400 mesh Quantifoil R1.2/1.3 grids with empty holes and 400 mesh Quantifoil R1.2/1.3 grids coated with a 2 nm thin carbon layer (Quantifoil Micro Tools GmbH, Germany). The grids were rendered hydrophilic by glow discharge using a Pelco easiGlow glow discharger (Ted Pella Inc. ...
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bioRxiv - Microbiology 2020Quote: ... Cells were concentrated by centrifugation and 3 μL aliquots of the cell suspension are applied to glow-discharged R2/2 200 mesh copper Quanti-foil grids (Quantifoil Micro Tools), the sample was pre-blotted for 30 seconds ...
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bioRxiv - Cell Biology 2020Quote: ... A 5 µl drop of the chemically fixed cell culture was deposited on a glow-discharged Quantifoil 200 mesh R2/2 electron microscopy grid (Quantifoil, Großlöbichau, Germany) pre-coated with a gold bead solution ...
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bioRxiv - Cell Biology 2020Quote: ... 3.5 µL of gel filtration column eluate (∼0.5 mg/ml) was applied to a holey carbon support (Quantifoil 2/1-4Cu-50) at ∼90% relative humidity ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µL aliquots of 0.1 mg/ml cross-linked ternary complexes were applied to freshly glow-discharged R2/2 Quantifoil 200 mesh copper grids (Quantifoil Micro Tools). Grids were blotted in a climate chamber set to 20 °C and at 95% humidity before plunge frozen in liquid ethane set at −183 °C using a Leica EM GP system (Leica Microsystems) ...
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Computational toolbox for ultrastructural quantitative analysis of filament networks in cryo-ET databioRxiv - Cell Biology 2021Quote: B16-F1 cells were cultured as described above and seeded onto 200 mesh gold holey carbon grids (R2/2-2C; Quantifoil Micro Tools). Prior to cell seeding ...
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bioRxiv - Cell Biology 2021Quote: ... Glow-discharged holey Quantifoil grids with an additional layer of continuous carbon (R2/1 + 2 nm C, Cu 200 mesh grid, Quantifoil Micro Tools) were used ...
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Mycobacteria form viable cell wall-deficient cells that are undetectable by conventional diagnosticsbioRxiv - Microbiology 2022Quote: ... of which 3-4 µL was applied on glow-discharged 200 mesh copper grids with an extra thick R2/2 carbon film (Quantifoil Micro Tools). Vitrification was performed using an automated Leica EM GP plunge-freezer ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μl of the resulting mixture was spotted onto glow-discharged copper EM grids (Quantifoil 300 Au mesh, R2/2 carbon film (Quantifoil Micro Tools). The grids were then blotted for 2 or 4 seconds (blot force −10 ...
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bioRxiv - Cell Biology 2022Quote: ... 3 µl of the microtubule sample was deposited at the surface of a glow-discharged holey carbon grid (Quantifoil R2/2, Cu200) in the temperature (35 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were applied to freshly glow-discharged (Edwards S150B, 45 s, 30−50 mA, 1.2 kV, 10−2 mbar) copper holey carbon grids (Quantifoil, Cu-R1.2/1.3) under 100% humidity at 4°C.
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bioRxiv - Biochemistry 2023Quote: 4.5 µL of freshly mixed Nup98 condensate and capsid mixture with protein A-gold (10 nm) were applied onto a glow-discharged Quantifoil R2/2 copper grid (Quantifoil Micro Tools). The grid was blotted in the front for 2.5 s at 15 °C with 90% relative humidity and then plunged into liquid ethane using a Lecia EM GP device (Lecia Microsystem) ...
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bioRxiv - Biochemistry 2024Quote: Grids for cryo-EM were prepared by applying 4 µL of sample on a glow-discharged Quantifoil 300 mesh Cu 2/1 grid (Quantifoil Micro Tools), blotting for 7.5 seconds ...
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bioRxiv - Microbiology 2020Quote: ... 3.5 μl of cell suspension from 80-100 μl of final volume were applied on glow discharged 200-mesh EM copper grids coated with R 2/1 holey SiO2 films (Quantifoil Micro Tool GmbH) and plunge frozen in liquid ethane at ~−184°C using a Leica EM GP grid plunger ...
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bioRxiv - Biophysics 2020Quote: ... 3.5ul of cell suspension were applied on 200-mesh copper grids coated with R 2/1 holey carbon or SiO2 films (Quantifoil Micro Tools GmbH) and plunge frozen in liquid ethane at ~-186°C using a Leica EM GP grid plunger ...
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bioRxiv - Immunology 2022Quote: ... detergent at a final concentration of 0.005 mM was added to each sample and 3 µl of that solution was immediately loaded onto plasma-cleaned QuantiFoil R 2/1 (400-mesh; Quantifoil Micro Tools GmbH) and UltrAuFoil R 1.2/1.3 grids (300-mesh ...
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bioRxiv - Biochemistry 2020Quote: ... 3 μl aliquots of purified mitoribsomes was incubated for 30 s on glow-discharged holey carbon grids (Quantifoil R2/2, 300 mesh, copper) pre-coated with a home-made continuous carbon film with thickness of ~ 27 Å ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μL of urease samples at 1.3 mg/mL with 1 mM BME or with 10 mM SHA were applied to freshly glow-discharged Quantifoil R2/2 holey carbon grids (Quantifoil Micro Tools, Germany). The grids were blotted for 4 seconds ...
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bioRxiv - Immunology 2020Quote: ... and 3 µl was immediately loaded onto plasma cleaned Quantifoil R 2/1 holey carbon copper gri (Cu, 400-mesh, Quantifoil Micro Tools GmbH). Blotted grids were plunge-frozen into nitrogen-cooled liquid ethane.
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bioRxiv - Biochemistry 2022Quote: ... and an aliquot of 2 μl of this sample was applied onto a plasma cleaned holey carbon grid (Quantifoil, R1.2/1.3, 200 mesh). The grid was blotted with Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: A 5 µl cell suspension containing 10 nm colloidal gold particles were deposited on the copper EM grids with holey carbon supported film (Quantifoil, 200mesh, R1/2). The EM grid were blotted with filter paper (Waterman ...
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bioRxiv - Molecular Biology 2022Quote: ... 2–3 μL of sample solution was applied to a holey carbon grid (Quantifoil R2/1, Mo 300 mesh; Quantifoil Micro Tools GmbH) covered with a thin amorphous carbon film at 4 °C with 100% humidity ...
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bioRxiv - Cell Biology 2022Quote: ... A 3 µl volume of the Kif5B suspension was first deposited at the surface of a glow-discharged holey carbon grid (Quantifoil R2/2, Cu200) in the temperature (23 °C ...
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bioRxiv - Microbiology 2023Quote: ... Both TBEV strain Neudoerfl and Hypr samples were vitrified in liquid ethane on holey carbon coated copper grids (Quantifoil 2/1, mesh 300, Quantifoil Micro Tools GmbH) using Vitrobot Mark IV (Thermo Fisher) ...
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bioRxiv - Biochemistry 2023Quote: ... 3µL of sample were frozen in liquid ethane on 2/1 Quantifoil Multi A holey carbon supported grids (Quantifoil, Multi A, 400 mesh) using Vitrobot Mark IV (FEI Company) ...
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bioRxiv - Biophysics 2023Quote: Three microliters of the purified GLV-HP or GLV-CAT sample was placed on a glow-discharged holey carbon grid (Quantifoil R2/2 or R2/1, Cu 300 mesh; Quantifoil Micro Tools GmbH) for 3 s blotting time at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... intact MVKCg1 yeasts cells resuspended in PBS were vitrified on Quantifoil Au R2/2 London Finder 300-mesh grids (Quantifoil Micro Tools GmbH) following a similar vitrification procedure as described above for crude membranes ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Grids were prepared by applying 3.5 μLs of the Z7757 complex or 2 μLs of the Lisuride complex to glow discharged UltrAuFoil holey gold grids (Quantifoil, Au300-R1.2/1.3). The girds containing Z7757 were then vitrified by plunge freezing into a 60/40 mixure of liquid ethane/propane using a Vitrobot mark IV (FEI ...
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bioRxiv - Biophysics 2024Quote: ... 4 μl sample was applied to each glow-discharged (30 s at 15 mA) QuantiFoil 2/1 Cu 300 grid (Quantifoil Micro Tools GmbH), pre-coated with graphene oxide according to Boland et al (21) ...
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bioRxiv - Biophysics 2024Quote: ... Cryo-EM grids were obtained by flash-freezing 3 µL of purified IPNV particles on glow-discharged holey carbon grids (Quantifoil R2/2, Cu 300 mesh; Quantifoil Micro Tools GmbH). The grids were plunge-frozen in liquid ethane after blotting for 3 s under 4°C ...
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bioRxiv - Cell Biology 2020Quote: 4 µl of the cultured yeast cells (OD600 of 0.4) were added to a glow-discharged (30 seconds at -30 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools GmbH, Jena, Germany), then the grid was rapidly plunge frozen in liquid ethane using a homemade plunge freezer and stored in liquid nitrogen until used ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 μl of protein sample (about 0.73 mg/ml) was applied onto a glow-discharged holey carbon grid (Quantifoil Au, R2/2, 300 mesh). After blotting for 3 s ...
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bioRxiv - Biophysics 2022Quote: ... an aliquot of 3 μL of sample was applied onto a glow-discharged holey carbon-coated copper grid (300 mesh, QUANTIFOIL® R 2/1) or holey gold grid (300 mesh ...
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bioRxiv - Biochemistry 2020Quote: ... 2.5 μL aliquots of purified Dnf1p–Lem3p or Dnf2p–Lem3p at a concentration of about 1.5 mg/mL were placed on glow-discharged holey carbon grids (Quantifoil Au R2/2, 300 mesh) and were flash-frozen in liquid ethane using a FEI Vitrobot Mark IV ...
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bioRxiv - Cell Biology 2022Quote: ... 4 µL of the mixture were pipetted onto a copper EM grid with holey carbon film (R2/2, 200 mesh, Quantifoil Micro Tools GmbH, Q43486) that had been freshly glow-discharged for 30 s at -30 mAmp ...