Labshake search
Citations for Quantifoil Micro Tools :
301 - 350 of 675 citations for 4 2 Methylimidazol 1 ylmethyl phenylamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... For plunge freezing the cells were pipetted (4 µl) on EM grids (gold/200mesh/R2.2 either UltrAufoil or Quantifoil) in a humidified chamber (98% RH) ...
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bioRxiv - Molecular Biology 2021Quote: ... and applied onto freshly glow-discharged 200 square mesh Ultrafoil R2/2 gold grids (Quantifoil). The samples were plunge-frozen with a FEI Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Freshly glow-discharged finder gold quantifoil grids (200 mesh R2/2 – Quantifoil MicroTools GmbH, Germany) were sterilized in 70% EtOH and placed in a glass-bottomed MatTek dish (MatTek Corporation ...
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bioRxiv - Microbiology 2021Quote: ... were applied to freshly washed and glow-discharged R2/2 holey carbon copper grids (Quantifoil). The grids were manually back blotted for 2 to 3s with filter paper ...
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bioRxiv - Biochemistry 2021Quote: ... Cryo-EM grids were prepared using glow discharged R2/2 Cu 200 mesh grids (Quantifoil) pre-floated onto custom-made 2.9 nm continuous carbon film ...
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bioRxiv - Microbiology 2023Quote: ... sample aliquots of 3 µL were applied onto UltrAuFoil R2/2 grids (300 mesh, Quantifoil). Grids were blotted for 1 s at 10°C with 100% relative humidity and immediately plunge frozen into liquid ethane using a Vitrobot Mark IV system (TFS) ...
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bioRxiv - Molecular Biology 2023Quote: ... 3.5 μL) were applied to precoated (2 nm) R1.2/1.3 carbon-supported copper grids (Quantifoil), blotted for 4-5 s at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... and 3 µL were applied to glow-discharged R2/2 holey carbon copper grids (Quantifoil). These grids were manually back blotted for 2 to 3s with filter paper ...
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bioRxiv - Biophysics 2022Quote: 3.5 μl of protein samples at 4-6 mg/ml were applied to glow-discharged QF R1.2/1.3 300 mesh gold grids (Quantifoil, Großlöbichau, Germany). Grids were blotted for 3 s at 4°C and 100% humidity and plunge-frozen into liquid ethane using FEI Mark IV Vitrobot (FEI ...
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bioRxiv - Plant Biology 2022Quote: ... then 4 µl of the sample was applied to glow-discharged holey carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh). The grid was blotted for 4s and then plunged into liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Cell Biology 2024Quote: ... 4 μl of the cell suspension was applied onto a glow-discharged holey SiO2 grid (R1/4, Au, 200 mesh, Quantifoil) mounted on in the plunger chamber ...
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bioRxiv - Molecular Biology 2023Quote: 1.8 × 105 A549 or A549-IFITM3 cells were seeded on glow-discharged 200 mesh gold grids with a holey SiO2 film with R1/4 spacing (Quantifoil) placed in a 35 mm dish and cultured in 2 ml complete growth medium (DMEM-F12 ...
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bioRxiv - Biochemistry 2023Quote: ... 4 μL of Fcμ–J–CD5L was applied to glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3, 300 mesh), blotted with filter paper in 100% humidity at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... 3μl was added to a glow-discharged R2/2 200 mesh holey carbon EM grid (Quantifoil) for 30 seconds and the sample was blotted for 1 second ...
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bioRxiv - Cell Biology 2022Quote: ... A volume of 2.5 μL was applied on glow-discharged UltrAuFoil Au200 R2/2 grids (Quantifoil), and excess liquid was removed by blotting for 3 s (blot force 5 ...
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bioRxiv - Biochemistry 2022Quote: ... 3 µL of purified PPSA was applied to glow-discharged holy grids (Quantifoil, Cu200, R2/2) for 30s ...
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bioRxiv - Biochemistry 2023Quote: The final high resolution data set was collected across 2 grids (Quantifoil 1.2/1.3 200 mesh) prepared with 1.8 mg mL-1 trimeric complex and 0.5 and 0.1 X CMC CHAPS ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantifoil R1.2/1.3 400-square mesh copper grids with a 2 nm continuous carbon layer (Quantifoil) were plasma-treated in a Pelco easiGlow glow discharging system (Ted Pella ...
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bioRxiv - Biophysics 2024Quote: ... Vero cells were seeded either on gold finder grids (200 mesh, R2/2, Quantifoil, Jena, Germany) or gold grids coated with lacey carbon film (XXBR Technology ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Three-hundred mesh R1.2/1.3 UltrAuFoil grids with a layer of 2 nm amorphous carbon (Quantifoil) were glow discharged in a Pelco easiGlow for 12 seconds under a 0.37 mBar vacuum with 25 mA current ...
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bioRxiv - Microbiology 2021Quote: ... Aliquots (4 μL) of the protein complex were placed on glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3, 300 mesh). The grids were blotted and flash-frozen in liquid ethane cooled by liquid nitrogen with a Vitrobot apparatus (Mark IV ...
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bioRxiv - Microbiology 2020Quote: Three μl of purified HKU1 HE (4 mg/ml) was dispensed on Quantifoil R1.2/1.3 200-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly glow discharged for 30 seconds at 20 mA using GloQube Glow Discharge system (Quorum Technologies) ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 μL of protein samples were immediately applied to discharged 300-mesh Au R1.2/1.3 grids (Quantifoil, Micro Tools GmbH, Germany). Grids were blotted for 4 s and plunged into liquid ethane using an FEI Mark IV Vitrobot operated at 8°C and 100% humidity ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 μL of the cells were applied to a glow-discharged 200 mesh holey carbon grid copper grid (Quantifoil R1.2/3) and vitrified in a liquid ethane on a Vitrobot Mark IV (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 4 μl of the sample was applied to glow discharged holey carbon-coated 200 mesh copper EM grids (Quantifoil Micro Tools), vitrified as above at 4°C and 100 % humidity.
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bioRxiv - Immunology 2024Quote: Neutrophils were stimulated with 100 nM LTB4 in mHBSS for 15 mins at 37 °C in 35 mm glass bottom dishes containing glow-discharged Au SiO2 R1/4 200 mesh grids (Quantifoil GmbH) precoated with 25 μg/ml fibrinogen in DPBS ...
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bioRxiv - Immunology 2024Quote: ... a 4 μL aliquot of protein complex was applied to glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3, 300-mesh) or graphene oxide (GO)-coated holey-carbon gold grids (EMR ...
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bioRxiv - Biochemistry 2021Quote: Three microliters of the h20S-PA26E102A-Opt5 solution were applied to R2/2 400-mesh grids (Quantifoil) that had been plasma treated for 25 s using a glow discharger (Electron Microscopy Sciences ...
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bioRxiv - Molecular Biology 2022Quote: ... 3.5 μL was applied to freshly glow-discharged Quantifoil R2/2 300-square-mesh gold grids (Quantifoil) in a Vitrobot IV (ThermoFisher ...
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bioRxiv - Biophysics 2020Quote: ... 2 μl apoferritin was applied to glow-discharged gold holey carbon 1.2/1.3 300-mesh grids (Quantifoil). Grids were blotted for 4 seconds at -2 force and vitrified in liquid ethane using a MarkIV Vitrobot (ThermoFisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... 3.5 μL was applied to freshly glow discharged Quantifoil R2/2 300-square-mesh gold grids (Quantifoil) in a Vitrobot IV (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... The samples were applied to a Quantifoil R2/2 Cu 300 mesh grid (Quantifoil Micro Tools GmbH), which had been freshly glow-discharged for 60 s at 10 mA using PIB-10 (Vacuum Device) ...
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bioRxiv - Microbiology 2024Quote: ... 3 μL of sample was loaded onto glow discharged holey carbon grids (Quantifoil, 300 mesh, R1/2). Samples were blotted for 3s and plunge frozen in liquid ethane using a Leica EM GP2 to obtain vitrified ice for imaging ...
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bioRxiv - Microbiology 2024Quote: A purified filament fraction (2 µL) was applied to a copper grid (R1.2/1.3 300 mesh; Quantifoil) and glow-discharged for 10 s using a JEC-3000FC Auto Fine Coater (JEOL) ...
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bioRxiv - Biochemistry 2024Quote: Samples were concentrated to 0.2 mg/ml or 1 mg/ml and prepared either on copper grids with holey carbon support and an additional layer of 2 nm continuous carbon (R1.2,1.3, Quantifoil) or on UltAufoil grids (R0.6/1.0 ...
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bioRxiv - Bioengineering 2022Quote: ... holey carbon grids (Quantifoil R2/1 grid: Cu 300mesh, M2951C-1-300) were hydrophilized by a JEC-3000FC Auto Fine Coater (JEOL ...
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bioRxiv - Molecular Biology 2021Quote: ... bremelanotide– or THIQ–bound MC4R–Gs complexes at ∼ 4 mg/mL were applied onto glow-discharged holey carbon grids (Quantifoil R1.2/1.3). Excess samples were blotted away and the grids were vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFischer Scientific).
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bioRxiv - Genomics 2020Quote: ... Vitrified specimens were prepared by adding 4 μl disome samples at the concentration of ~ 150 nM to a glow-discharged holey carbon grid (Quantifoil R1.2/1.3), which was covered with a freshly made thin carbon film ...
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bioRxiv - Cell Biology 2020Quote: ... The MEFs were applied onto glow-discharged EM grids with a silicon-oxide support film (R1/4, Au mesh; Quantifoil, Jena, Germany). After incubation ...
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bioRxiv - Cell Biology 2022Quote: The cryo-EM grid was prepared by applying 3 μl of the protein samples (4 – 6 mg/ml) to glow-discharged Quantifoil R1.2/1.3 300-mesh gold holey carbon grids (Quantifoil, Micro Tools GmbH, Germany). Grids were blotted for 4.0 seconds under 100% humidity at 4°C before being plunged into the liquid ethane using a Mark IV Vitrobot (FEI) ...
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bioRxiv - Cell Biology 2023Quote: ... Four µL of the cells were subsequently deposited onto Quantifoil R2/4 200 mesh copper grids (Quantifoil Micro Tools GmbH, Jena, Germany), which were then manually blotted from the back with Whatman® Grade 1 filter paper for approximately 3 – 5 seconds ...
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bioRxiv - Biophysics 2023Quote: ... 4 μl suspended cells were loaded onto the front side of glow-discharged holy-carbon copper grids (Quantifoil R1.2/1.3, 200 mesh), and 2 μl medium was subsequently added to the back side of the grids ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 µL sample was applied to glow-discharged gold holey carbon 1.2/1.3 300-mesh grids (Quantifoil) coated in-house with graphene oxide55-57 ...
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bioRxiv - Biochemistry 2021Quote: ... A volume of 2.5 μL of each sample was applied on glow-discharged UltrAuFoil Au300 Rl.2/1.3 grids (Quantifoil), and excess liquid was removed by blotting for 3 s (blot force 5 ...
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bioRxiv - Biochemistry 2022Quote: ... 2 μl retromer at a concentration between 80-100 μg/ml was applied to freshly glow discharged CF-2/2-2C C-Flat grids (Protochips, Morrisville, NC) or Quantifoil R 1.2/1.3 300 mesh copper grids (Quantifoil Micro Tools GmbH ...
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bioRxiv - Microbiology 2022Quote: ... 3.5 μL of cell suspension was applied on glow-discharged copper or gold EM grids (R2/2, Quantifoil) and automatically back-blotted (Weiss et al 2017 ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 μl of the resultant mixture was applied to glow-discharged (EasiGlow Pelco) R2/2 Quantifoil grids (Quantifoil). Excess sample was blotted away with a FEI Vitrobot (IV ...
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bioRxiv - Evolutionary Biology 2021Quote: Molybdenum R1.2/1.3 and R2/2 200-mesh grids with a holey carbon support film (Quantifoil, Jena, DE) were glow discharged for 40 s under high vacuum shortly before sample application ...
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bioRxiv - Biochemistry 2021Quote: ... 2 uL of crystal slurry was applied to each side of a glow-discharged holey carbon grid (Quantifoil, R 2/1 200 mesh Cu ...
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bioRxiv - Biochemistry 2021Quote: ... 2 uL of crystal slurry was applied to each side of a glow-discharged holey carbon grid (Quantifoil, R 1/4 300 mesh Cu ...