Labshake search
Citations for Quantifoil Micro Tools :
251 - 300 of 384 citations for N6 2 aminoethyl 9H purine 2 6 diamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 3.5 µL of sample was applied to a glow discharged UltrAufoil holey gold grids (R2/2, 200 mesh, Quantifoil). Grid were blotted for 5 s at 4°C and 95% relative humidity before freezing in liquid ethane using a Vitrobot Mark IV (FEI) ...
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bioRxiv - Biophysics 2024Quote: ... 4 µL of resuspended solution was applied to freshly glow-discharged Quantifoil R2/2 mesh 300 gold grids (Quantifoil) in a Vitrobot IV (ThermoFisher ...
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bioRxiv - Biochemistry 2020Quote: ... 3 μL of the concentrated RdRp-RNA complex were mixed with 0.5 μl of octyl ß-D-glucopyranoside (0.003% final concentration) and applied to freshly glow discharged R 2/1 holey carbon grids (Quantifoil). The grids were blotted for 5 seconds using a Vitrobot MarkIV (Thermo Fischer Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... 3 µL of the concentrated RdRp-RNA complex were mixed with 0.5 µl of octyl ß-D-glucopyranoside (0.003% final concentration) and applied to freshly glow discharged R 2/1 holey carbon grids (Quantifoil). The grids were blotted for 7 seconds with blot force 5 using a Vitrobot MarkIV (Thermo Fischer Scientific ...
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bioRxiv - Biophysics 2020Quote: ... and urine were applied in 3 µl-volumes onto glow-discharged Quantifoil Au R2/2 holey carbon 300 mesh grids (Quantifoil). Grids were blotted for 2.0 s and plunged into liquid ethane cooled by liquid nitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... 3.0 μL of sample was applied to glow-discharged 200-mesh copper 2 nm C R1.2/1.3 cryo-EM grids (Quantifoil Micro Tools), blotted 1 s at 100 % humidity ...
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bioRxiv - Biochemistry 2022Quote: ... 2.5 μL of sample was applied to glow-discharged 200-mesh copper 2 nm C R1.2/1.3 cryo-EM grids (Quantifoil Micro Tools), blotted 3 s at 75 % humidity ...
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bioRxiv - Biophysics 2020Quote: ... 3 μL of the concentrated RdRp-RNA complex were mixed with 0.5 μl of octyl ß-D-glucopyranoside (0.003% final concentration) and applied to freshly glow discharged R 2/1 holey carbon grids (Quantifoil). Prior to flash freezing in liquid ethane ...
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bioRxiv - Cell Biology 2022Quote: Cells were applied onto galectin-8 coated EM grids with carbon support film (R2/2, Au mesh; Quantifoil, Jena, Germany). After 15-20 min incubation ...
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bioRxiv - Cell Biology 2021Quote: HeLa Doxycycline-inducible stable cell lines were grown for approximately 48 h on holey carbon gold EM grids (200 mesh, R2/2, Quantifoil) prior to plunge freezing ...
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bioRxiv - Molecular Biology 2020Quote: ... a 2.2-µL aliquot of this sample was applied to a plasma cleaned holey carbon grid (R2/1, 200 mesh; Quantifoil) or Graphene Oxide-Lacey Carbon grid (300 mesh ...
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bioRxiv - Microbiology 2020Quote: ... 3,5 μl of the culture was mixed with 10 nm gold fiducial markers (Cell Microscopy Core, UMC Utrecht) and applied onto glow-discharged R2/2 200 mesh holey carbon EM grids (Quantifoil). The grid was then rapidly plunged in liquid ethane using the Leica GP automated freeze-plunger ...
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bioRxiv - Molecular Biology 2020Quote: 3.5 µl of the concentrated sample were applied to glow discharged copper grids with holey carbon support and a 2 nm continuous carbon layer (R3/3, Quantifoil). Grids were blotted in a Vitrobot Mark IV (FEI Company ...
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bioRxiv - Evolutionary Biology 2020Quote: ... was applied onto a glow-discharged (20 mA for 30 seconds) holey-carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ~3 nm thickness ...
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bioRxiv - Biochemistry 2021Quote: ... 3.5 μL sample was applied onto a freshly glow-discharged 200-mesh copper 2 nm C R1.2/1.3 grids (Quantifoil Micro Tools). The grids were blotted for 1 s and plunge-vitrified in liquid ethane ...
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bioRxiv - Biophysics 2020Quote: ... Mycoplasma pneumoniae strain M129(ATCC 29342)cells were grown on 200 mesh gold grids coated with a holey carbon support (R 2/1, Quantifoil). Cells were cultivated at 37 °C in modified Hayflick medium ...
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bioRxiv - Microbiology 2021Quote: ... An aliquot (3 μl) of this mixture was applied to a glow-discharged holey carbon grid (QF R2/2 Cu, QUANTIFOIL), blotted with Whatman® No ...
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bioRxiv - Biochemistry 2022Quote: ... 2–2.5 µl of protein solution was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3 Au 200 mesh). The endogenous samples were applied onto grids containing an additional thin layer of continuous carbon support (Quantifoil R1.2/1.3 Cu 200 mesh + 2 nm C ...
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bioRxiv - Microbiology 2022Quote: ... and 4 µl of the mixture was applied to a glow-discharged holey-carbon copper EM grid (R2/1 or R2/2, Quantifoil). The grid was automatically blotted from the backside for 4-6 s in a Mark IV Vitrobot by using a Teflon sheet on the front pad ...
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bioRxiv - Microbiology 2022Quote: ... mixed with 10 nm protein A-coated colloidal gold (Aurion) and applied onto a glow-discharged EM grid (200 mesh, R 2/1, Quantifoil) prior to plunge-freezing with a Leica EM GP2 automatic plunge-freezer.
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bioRxiv - Cell Biology 2024Quote: ... 4 × 104 MDM cells were seeded on glow discharged and UV-light sterilized holey carbon grids (R2/2, Au, 200 mesh, Quantifoil), which were placed in a glass-bottomed ‘microwell’ of 35-mm MatTek dish (MatTek ...
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bioRxiv - Cell Biology 2024Quote: ... 3 µl of cell suspension was applied to a freshly grown discharged holey carbon grid (Quantifoil® #200, R2/2). Grids were immediately blotted from the back side using Whatman filter paper #1 (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: Cells were blotted on EM grids (R 2/1 holey carbon 200 films on Cu 200 mesh, Quantifoil Micro Tools). The grids were first glow-discharged and 4 µl of trypanosome wild-type culture (2-4 · 107 cells/ml ...
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bioRxiv - Microbiology 2023Quote: ... and 4 µl of the mixture was applied to a glow-discharged holey-carbon copper EM grid (R2/1 or R2/2, Quantifoil). The grid was automatically backside blotted for 4-6s in a Mark IV Vitrobot (Thermo Fischer Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples for cryo-ET were deposited onto 300-mesh holey carbon grids (Quantifoil Micro Tools, R2/2 X-103-Cu300) and samples for cryo-SPA were deposited onto 200-mesh holey carbon grids (Quantifoil Micro Tools ...
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bioRxiv - Cell Biology 2023Quote: ... An aliquot of 3 ul cell suspension was applied to the glow-discharged holey carbon-coated copper (R 2/1, 200 mesh) (Quantifoil) and blotted for 9 seconds by Leica GP2 (Leica Microsystems) ...
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bioRxiv - Biochemistry 2023Quote: ... 3 µL of each sample was applied to glow-discharged (PELCO easiGlow, 15 mA, 2 min) holey carbon grid (Quantifoil R1.2/1.3 on gold or copper 200 mesh support) ...
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bioRxiv - Biophysics 2023Quote: ... HeLa cells were trypsinized and seeded at a density of 0.5 – 0.7 X 105 cells/mL on carbon-coated gold Quantifoil grid (200 mesh, R 2/1, holey SiO2 film; Quantifoil Micro Tools GmbH ...
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bioRxiv - Biophysics 2023Quote: A 3 µL aliquot of purified CDTbD623A/D734Aprotein sample at a concentration of 30 µM was applied to a glow discharged QUANTIFOIL® copper R 1.2/1.3 grid with a 2 nm thin extra carbon layer (QUANTIFOIL, GMBH). For calcium depleted CDTb (28 µM) ...
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bioRxiv - Biochemistry 2023Quote: 2.4 µL of sample were applied to a freshly glow discharged 200-mesh Cu + 2 nm C R1.2/1.3 grid (Quantifoil Micro Tools), blotted for 6 s at 100% humidity ...
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bioRxiv - Biophysics 2023Quote: ... 3 or 3.5 μl of LRRK2RCKW:inhibitor:E11 DARPin complex were applied to a glow-discharged UltraAuFoil Holey Gold 200 mesh R2/2 grid (Quantifoil, Catalog # Q250AR2A) and incubated in a FEI Vitrobot IV chamber at 4°C and 95% humidity for 20 sec ...
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bioRxiv - Microbiology 2024Quote: ... A 4.5 µL droplet of the culture was applied to glow discharged copper grids (Quantifoil R2/2, Quantifoil Micro Tools) and blotted for 2.5 s in a 95% humidity chamber at 20 °C ...
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bioRxiv - Microbiology 2024Quote: ... The mixture was added onto glow-discharged copper R2/2 Quantifoil holey carbon grids (Quantifoil Micro Tools GmbH, Jena, Germany). Grids were blotted for 6-8 seconds (under 100% humidity conditions ...
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bioRxiv - Biochemistry 2024Quote: ... was applied to glow-discharged (GloQube® Plus, Quorum, UK) Quantifoil R1.2/1.3 or R2/2 mesh 200 copper holey carbon grid (Quantifoil, Germany), blotted under 100% humidity at 4°C for 6–8 s ...
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bioRxiv - Cell Biology 2024Quote: HeLa WT cells or HeLa cells stably expressing Apaf1-GFP were plated on 200 mesh gold EM grids with a holey carbon film R2/2 (Quantifoil). HeLa WT cells were transfected with Apaf1-SNAP-tag ...
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bioRxiv - Microbiology 2024Quote: ... and 4 µl of the mixture was applied to a glow-discharged holey-carbon copper EM grid (R2/1 or R2/2, [Quantifoil]). The grid was automatically blotted from the backside for 4-6 s in a Mark IV Vitrobot by using a Teflon sheet on the front pad ...
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bioRxiv - Microbiology 2024Quote: ... A 4.5 µL droplet of the culture was applied to glow discharged copper grids (Quantifoil R2/2, Quantifoil Micro Tools) and blotted for 2.5 s in a 95% humidity chamber at 20 °C ...
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bioRxiv - Microbiology 2024Quote: ... We initially prepared frozen-hydrated viruses deposited onto Quantifoil R2/2 or R1.2/1.3 grids (Quantifoil Micro tools GmbH, Germany) or glow discharged Lacey grid (Ted Pella Inc. ...
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bioRxiv - Neuroscience 2024Quote: Plunge-freezing VLPs: 3 µL of crude enriched VLP resuspension was applied to Holey copper R2/2 grids (Quantifoil GmBH) glow-discharged at 5mA 30s using an EasiGlow system (Pelco) ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µL of 10 µM 11-16Ub[K48]-GFP-35 was pipetted on a holey carbon R2/2 Cu200 grid (Quantifoil) that was plasma cleaned by a SCD005 sputter coater (Bal-Tec ...
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bioRxiv - Cell Biology 2024Quote: ... 3 µL of LRRK2RCKW:RN277:E11 DARPin complex were applied to a glow-discharged UltraAuFoil Holey Gold 200 mesh R2/2 grid (Quantifoil, Q250AR2A) and incubated in a FEI Vitrobot IV chamber at 4 °C and 95 % humidity for 20 sec ...
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bioRxiv - Biochemistry 2024Quote: HeLa cells stably expressing either HRS-mScarlet or HRS-mCherry were grown on 200 mesh gold EM grids with a holey carbon film R2/2 (Quantifoil). Cells were incubated with 50 ng/ml EGF- Alexa 647 ...
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bioRxiv - Biophysics 2020Quote: ... samples were stored on ice and applied to the cryo-grids (2 nm precoated Quantifoil R3/3 holey carbon supported grids) at a concentration of 10 μM and vitrified using a Vitrobot Mark IV (FEI) ...
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bioRxiv - Plant Biology 2021Quote: ... 3 µl of suspended cells in culture medium were applied to the front side of glow-discharged R2/2 carbon-coated copper grids (Quantifoil MicroTools), with additional 1 µL cells added to the back side ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of the sample mixture was applied to a freshly glow-discharged copper R2/2 200 grid (Quantifoil Micro Tools), pre-blotted for 30 seconds ...
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bioRxiv - Biochemistry 2021Quote: ... Three microliters of the protein solution were applied on a glow-discharged holey carbon grids (200 mesh Quantifoil R2/2 molybdenum), which had been treated with H2 and O2 mixtures in a Solarus plasma cleaner (Gatan ...
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bioRxiv - Cell Biology 2022Quote: ... The IL-11Δ10/IL-11RαD1-D3/gp130D1-D3 complex (0.5 mg/mL) was blotted onto UltrAuFoil grids (R2/2, Quantifoil Micro Tools GmbH) that had been subjected to glow discharge (15 mA for 30 s) ...
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bioRxiv - Microbiology 2023Quote: ... sample were placed onto glow-discharged Quantifoil R2/1 Cu300 holey carbon grids (1 µm spacing, 2 µm holes, copper mesh) (Quantifoil, Germany), blotted for 3.5 s before flash-freezing in liquid ethane using a Vitrobot mark IV (FEI ...
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bioRxiv - Biophysics 2023Quote: ... 3 to 3.5 μl of complex was applied to a glow-discharged UltrAuFoil Holey Gold 200 mesh R2/2 grid (Quantifoil, Catalog # Q250AR2A) and incubated in a FEI Vitrobot IV chamber at 4°C and 95% humidity for 20 sec ...
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bioRxiv - Biochemistry 2024Quote: ... 3 µL of suspended cells in culture medium were applied to the front side of glow-discharged R2/2 carbon-coated copper grids (Quantifoil MicroTools), with additional 1 µL cells added to the back side ...