Labshake search
Citations for Quantifoil Micro Tools :
201 - 250 of 281 citations for Human Xylosyltransferase 1 XYLT1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and 4 µl of the mixture was applied to a glow-discharged holey-carbon copper EM grid (R2/1 or R2/2, Quantifoil). The grid was automatically backside blotted for 4-6s in a Mark IV Vitrobot (Thermo Fischer Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... An aliquot of 3 ul cell suspension was applied to the glow-discharged holey carbon-coated copper (R 2/1, 200 mesh) (Quantifoil) and blotted for 9 seconds by Leica GP2 (Leica Microsystems) ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μl of protein at a concentration of approximately 10 mg ml−1 were loaded onto glow-discharged holey carbon grids (Quantifoil Au 300 mesh R1.2/1.3 or Quantifoil Cu/Rh 300 mesh R1.2/1.3) ...
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bioRxiv - Biochemistry 2023Quote: ... 3 µl of the VIF-ΔT solution was applied onto glow-discharged holey carbon EM grids (Cu R2/1, 200 mesh, Quantifoil), which were subsequently blotted manually and plunge frozen in liquid ethane.
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bioRxiv - Biochemistry 2023Quote: ... 2.8 µl of F-actin sample was applied to a glow-discharged R2/1 Cu 300 mesh holey-carbon grid (Quantifoil). Excess solution was blotted away and the grids were plunge frozen in liquid ethane or a liquid ethane/propane mixture using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... HeLa cells were trypsinized and seeded at a density of 0.5 – 0.7 X 105 cells/mL on carbon-coated gold Quantifoil grid (200 mesh, R 2/1, holey SiO2 film; Quantifoil Micro Tools GmbH ...
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bioRxiv - Biophysics 2023Quote: ... 4 µL fresh sample (0.48 mg/mL) was applied on glow-discharged Au holey carbon grids (Quantifoil R 0.6/1) and the following operation were the same as describe above for preparing the sample of gMCM8/9 complex.
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bioRxiv - Cell Biology 2023Quote: 3 µl of prepared complexes at a concentration of approximately 1 mg/mL was applied to UltrAuFoil 300 mesh gold R1.2/1.3 grids (Quantifoil Micro Tools), freshly glow discharged with an Edwards S150B glow discharger for 1 min 15 sec ...
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bioRxiv - Biochemistry 2024Quote: ... using 3 µL of the samples applied to previously glow discharged R 2/1 Holey Carbon Cu 200 mesh EM grids (Quantifoil). Samples were 10 µM FtsZ with or without ZapD or mZapD at different concentrations specified in each case (0 – 60 µM) ...
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bioRxiv - Bioengineering 2022Quote: ... A total of 3.5 μL of TP–AG treated MT samples was applied to a glow discharged holey carbon grid (Quantifoil R2/1). The grids were blotted ...
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bioRxiv - Plant Biology 2020Quote: ... 4 μL of cell culture was blotted onto R 1/4 SiO2-coated 200-mesh gold EM grids (Quantifoil Micro Tools) and plunge frozen in a liquid ethane/propane mixture ...
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bioRxiv - Cell Biology 2021Quote: ... a thin layer of homemade continuous carbon was floated on top of individual Quantifoil R3.5/1 200 mesh gold grids (Quantifoil Micro Tools). After drying ...
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bioRxiv - Cell Biology 2020Quote: ... 3 μl of samples were applied to glow-discharged Quantifoil R 0.6/1 and R 1.2/1.3 grids (Quantifoil Micro tools GmbH, Germany), blotted for 3 s and then flash frozen in liquid ethane using the automatic plunge freezer EM GP2 at 100% relative humidity ...
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bioRxiv - Cell Biology 2021Quote: MEFs were grown to ~80% confluency on glow-discharged holey carbon EM grids (R2/1, Au 200 mesh; Quantifoil, Jena, Germany) prior to preparation for cryo-ET analysis ...
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bioRxiv - Molecular Biology 2021Quote: ... cells in suspension (4 μl of ~1,000 cells per μl) were blotted onto R2/1 carbon-coated 200-mesh copper grids (Quantifoil Micro Tools) and plunge frozen in a liquid ethane/propane mixture ...
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bioRxiv - Immunology 2022Quote: A 3.5 μL sample of concentrated pNO-decorated CLPs was applied to negatively glow discharged (25 mA, 40 s) R3.5/1 quantifoil copper grids (Quantifoil Micro Tools). The excess of solution was blotted using a Vitrobot Mark IV (FEI ...
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bioRxiv - Biochemistry 2023Quote: ... at a concentration of around 2.5 mg ml−1 were applied to glow-discharged 300-mesh Au grids (Quantifoil, R1.2/1.3). Excessive sample was removed by blotting with filter paper for 3.5 s before plunge-freezing in liquid ethane using a FEI Vitrobot Mark IV at 100% humidity and 8 °C.
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bioRxiv - Biochemistry 2023Quote: MEFs were grown to ∼80% confluency on glow-discharged holey carbon EM grids (R2/1, Au 200 mesh; Quantifoil, Jena, Germany) prior to preparation for cryo-ET analysis ...
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bioRxiv - Biophysics 2024Quote: Approximately 3□μL of the sample solution were put on newly glow-discharged TEM grids (Quantifoil R2/1, Cu, mesh 200) and plunge-frozen in liquid ethane using the Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... of purified proteins of SARS-CoV2-ST or SARS-CoV2-STN were loaded onto glow-discharged (60 s at 20 mA) holey carbon Quantifoil grids (R2/1, 200 mesh, Quantifoil Micro Tools) using a Vitrobot Mark IV (ThermoFisher Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: ... 25 mM Tris at pH 8.0 to a concentration of 2 mg/mL to a glow-discharged (1 min at 5 mA) holey carbon grid (Quantifoil Q225-CR1.3). This was followed by plunge-freezing in liquid ethane using an FEI Vitribot Mark IV (100% humidity ...
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bioRxiv - Plant Biology 2019Quote: ... 4 µL of mat3-4 cell culture was blotted onto R2/1 carbon-coated 200-mesh copper EM grids (Quantifoil Micro Tools) and plunge frozen in a liquid ethane/propane mixture ...
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bioRxiv - Microbiology 2019Quote: Cells of strain Pla133T were mixed with the same volume of BSA-stabilized 15 nm colloidal gold solution (Aurion) and placed on holey carbon-coated 200 mesh copper grids (R2/1, Quantifoil, Jena, Germany) immediately before thin-film vitrification by plunge-freezing in liquid propane (63%)/ethane (37% ...
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... A 3.5 µL virus sample solution was applied onto a glow discharged copper grid coated with holey carbon (R2/1; Quantifoil, Jena, Germany) prior to plunge-freezing ...
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bioRxiv - Cell Biology 2020Quote: ... 3.5 µL of gel filtration column eluate (∼0.5 mg/ml) was applied to a holey carbon support (Quantifoil 2/1-4Cu-50) at ∼90% relative humidity ...
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bioRxiv - Cell Biology 2021Quote: ... Glow-discharged holey Quantifoil grids with an additional layer of continuous carbon (R2/1 + 2 nm C, Cu 200 mesh grid, Quantifoil Micro Tools) were used ...
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bioRxiv - Biophysics 2023Quote: ... 3 μl of medium containing one isolated muscle fibre was placed on the glow-discharged grid (Quantifoil R2/1 Au 200 mesh). After incubation for 60 seconds at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Protein-DNA complexes were applied to ultra-gold foil grids with holey gold support and 300 mesh size (R 0.6/1,Quantifoil Micro Tools GmbH); grids were flash-frozen as described [21] and were stored in liquid nitrogen until use.
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bioRxiv - Cell Biology 2023Quote: ... 4 µL of cell suspension was applied on 200-mesh R ¼ SiO2-film covered gold grids or 200-mesh R2/1 carbon-film covered copper grids (Quantifoil Micro Tools) (for P ...
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bioRxiv - Microbiology 2023Quote: ... 3µL of each sample was was pipetted onto a freshly glow-discharged Quantifoil R2/1 grid (Quantifoil Micro Tools GmbH, Großlöbicha, Germany), then blotted to remove excess sample and plunge-frozen into a mixture of liquid ethane and propane cooled in a bath of liquid nitrogen using a Vitrobot Mk 4 cryo-plunger (41) ...
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bioRxiv - Cell Biology 2024Quote: ... the sample was gently resuspended and 4 µl was transferred to a glow-discharged holey carbon R2/1 copper grid (Quantifoil Micro Tools) suspended in the chamber of a Vitrobot (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Peak fractions were concentrated to ∼3.2 mg/mL and 3.5 μL of concentrated sample immediately applied to a Quantifoil R2/1 300 copper mesh grid (Quantifoil Micro Tools GmbH), which had been glow discharged in a GloQube Plus glow discharger for 45 seconds at 25 mA and coated with graphene oxide (Sigma Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... 3.5 μl of cell suspension from 80-100 μl of final volume were applied on glow discharged 200-mesh EM copper grids coated with R 2/1 holey SiO2 films (Quantifoil Micro Tool GmbH) and plunge frozen in liquid ethane at ~−184°C using a Leica EM GP grid plunger ...
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bioRxiv - Biophysics 2020Quote: ... 3.5ul of cell suspension were applied on 200-mesh copper grids coated with R 2/1 holey carbon or SiO2 films (Quantifoil Micro Tools GmbH) and plunge frozen in liquid ethane at ~-186°C using a Leica EM GP grid plunger ...
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bioRxiv - Biochemistry 2022Quote: ... An aliquot of 3.5 μl of sample was placed on the glow-discharged cryo-EM grid (Quantifoil R1.2/1.3 or Quantifoil R2/1), blotted and plunge-frozen in liquid ethane using a Mark VI Vitrobot instrument maintained at 100% humidity with blot force 20 and blot time of 3 seconds ...
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bioRxiv - Molecular Biology 2021Quote: ... DRD1-SKF81297-LY3154207-miniGs-Nb35 complex at the concentration of 13 mg mL−1 were applied individually onto glow-discharged holey carbon grids (Quantifoil, Au200 R1.2/1.3) in a Vitrobot chamber (FEI Vitrobot Mark IV) ...
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bioRxiv - Biophysics 2021Quote: To provide extra support for cell growth 5-6 nm of carbon was evaporated onto Quantifoil Au 200 R2/1 or R2/2 grids (Quantifoil Micro Tools GmbH). Grids were then glow discharged and incubated in supplemented RPMI-1640 overnight in the cell incubator ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of the sample was applied to a glow-discharged R2/1 300 mesh holey carbon copper grid (Quantifoil Micro Tools GmbH) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of diluted sample was applied to a glow-discharged (R2/1 300 mesh holey carbon copper grid (Quantifoil Micro Tools GmbH) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Immunology 2022Quote: ... detergent at a final concentration of 0.005 mM was added to each sample and 3 µl of that solution was immediately loaded onto plasma-cleaned QuantiFoil R 2/1 (400-mesh; Quantifoil Micro Tools GmbH) and UltrAuFoil R 1.2/1.3 grids (300-mesh ...
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bioRxiv - Biochemistry 2022Quote: Three microliters of Ct80S-RAC pull-out sample at 200 nM concentration was applied on holey carbon grids (Quantifoil R2/1 grid, Quantifoil Micro Tools, GmbH) and plunged-frozen into liquid ethane using a Vitrobot (FEI) ...
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bioRxiv - Microbiology 2020Quote: Usually 5 μL of liquid culture (OD680 ~ 0.3) was pipetted onto holey carbon-coated copper grids (R2/1, 200 mesh, Quantifoil Micro Tools, Jena, Germany). The grids were blotted from the reverse side and immediately plunged into the liquid ethane at liquid nitrogen temperature atmosphere using a Vitrobot ...
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bioRxiv - Immunology 2020Quote: ... and 3 µl was immediately loaded onto plasma cleaned Quantifoil R 2/1 holey carbon copper gri (Cu, 400-mesh, Quantifoil Micro Tools GmbH). Blotted grids were plunge-frozen into nitrogen-cooled liquid ethane.
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bioRxiv - Microbiology 2020Quote: ... 3.5 microliters of the purified virus sample was pipetted onto a glow-discharged R2/1 Quantifoil grid (Quantifoil Micro Tools GmbH, Jena, Germany), blotted for 2.5 seconds ...
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bioRxiv - Microbiology 2022Quote: ... 5 µl of bacteria diluted to OD = 0.15 in PBS were deposited on a glow-discharged Quantifoil R2/1 holey carbon grids (Quantifoil Micro Tools GmbH, Germany) and 3 µl gold particles (10 nm BSA tracer ...
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bioRxiv - Biochemistry 2022Quote: ... three microliters of concentrated phage particles were added to a freshly glow-discharged Quantifoil R2/1 grid (Quantifoil Micro Tools GmbH, Großlöbicha, Germany) and plunge-frozen with a Vitrobot Mk IV into a 50:50 mixture of liquid ethane:propane52
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bioRxiv - Microbiology 2022Quote: Three-microliter aliquots of all AdSDV capsid populations (~1 mg/mL) were applied to glow-discharged Quantifoil holey carbon grids with a thin layer of carbon (Quantifoil Micro Tools GmbH) and vitrified using a Vitrobot Mark IV (FEI ...
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bioRxiv - Microbiology 2022Quote: ... 5 µL of the mixture was placed onto freshly glow discharged holey carbon grids (Quantifoil™ R 2/1 on 200 copper mesh) for 1 minute ...
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bioRxiv - Molecular Biology 2022Quote: ... 2–3 μL of sample solution was applied to a holey carbon grid (Quantifoil R2/1, Mo 300 mesh; Quantifoil Micro Tools GmbH) covered with a thin amorphous carbon film at 4 °C with 100% humidity ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μL of sample (10 μM EGFP in PBS) was deposited onto glow-discharged grids (Electron Microscopy Science and Quantifoil – see Table 1), prior to blotting the grids from the sample-side for 3 seconds.