Labshake search
Citations for Quantifoil Micro Tools :
201 - 250 of 346 citations for 3 Bromo 4 chloro 5 methoxypyridine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 2.7 μl of freshly purified sample was applied onto glow-discharged (30 s, 5 mA) grids (Quantifoil R 0.6/1 300 mesh Cu or Ultrafoil 0.6/1 300 mesh Au ...
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bioRxiv - Biochemistry 2023Quote: 3.5 μL of LRRK2RCKW:E11 complex was applied to glow-discharged UltraFoil 300 mesh R1.2/1.3 grids (Quantifoil) and incubated in a FEI Vitrobot IV at 4°C and 95% humidity for 20 sec ...
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bioRxiv - Biochemistry 2020Quote: ... KBP was diluted to 0.3 mg/ml in KBP dilution buffer and 4 μl were applied to glow-discharged 1.2/1.3 AuFoil gold grids (Quantifoil®). For the third approach ...
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bioRxiv - Biophysics 2021Quote: ... 4 µl of this mixture was applied on a glow-discharged Quantifoil R2/2 grid (Quantifoil Micro Tools GmbH) and flash-frozen in liquid ethane using a Vitrobot Mark II device (FEI) ...
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bioRxiv - Molecular Biology 2021Quote: Purified mtLSU or 55S mitoribosome samples (4 μL) were applied to freshly glow discharged R 3.5/1 holey carbon grids (Quantifoil) that were precoated with a 2-3 nm carbon layer using a Leica EM ACE600 coater ...
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bioRxiv - Microbiology 2022Quote: ... A 4□μl volume of SsoCsm complex was applied to holey carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh) covered by a graphene oxide layer (27) ...
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bioRxiv - Microbiology 2024Quote: ... 4 μL of the cell mixture was applied three times onto glow-discharged copper EM grids (R2/2, Quantifoil), followed by plunge freezing into liquid ethane/propane ...
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bioRxiv - Biophysics 2024Quote: ... 4 µL of resuspended solution was applied to freshly glow-discharged Quantifoil R2/2 mesh 300 gold grids (Quantifoil) in a Vitrobot IV (ThermoFisher ...
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bioRxiv - Biophysics 2024Quote: ... For plunge freezing the cells were pipetted (4 μl) on EM grids (gold/200mesh/R2.2 either UltrAufoil or Quantifoil) in a humidified chamber (98% RH) ...
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bioRxiv - Cell Biology 2024Quote: ... 3.5 µl of 4 mg/mL proteasomal complexes was applied to glow-discharged R0.6/1 UltrAuFoil 300 mesh grids (Quantifoil) and frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... For plunge freezing the cells were pipetted (4 µl) on EM grids (gold/200mesh/R2.2 either UltrAufoil or Quantifoil) in a humidified chamber (98% RH) ...
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bioRxiv - Immunology 2022Quote: ... were incubated for 3 min at room temperature and then loaded onto the glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3). The grids were washed using the buffer containing 20 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 μL aliquots of the cell suspension were applied to glow-discharged R2/2 200 mesh copper Quantifoil grids (Quantifoil Micro Tools), the sample was pre-blotted for 30 seconds ...
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bioRxiv - Biophysics 2021Quote: ... 3 μl of samples were applied onto a glow-discharged gold grids covered with holey carbon film (Quantifoil, 300 mesh 1.2/1.3) and blotted using a Vitrobot Mark IV (FEI Company ...
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bioRxiv - Biochemistry 2022Quote: A 3 µL of the protein solutions was applied onto the glow-discharged holey carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh). The grids were plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: The purified tirzepatide–GLP-1R–Gs–Nb35 complex (3 μL at about 20 mg/mL) was applied to a glow-discharged holey carbon grid (Quantifoil R1.2/1.3) and blotted subsequently ...
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bioRxiv - Microbiology 2020Quote: ... 3.5 μl of the freshly prepared mixture of the soluble S trimer and monomeric ACE2 (1:3 molar ratio) at ~1 mg/ml was applied to a 1.2/1.3 Quantifoil grid (Quantifoil Micro Tools GmbH, Germany), which had been glow discharged with a PELCO easiGlow™ Glow Discharge Cleaning system (Ted Pella ...
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bioRxiv - Cell Biology 2022Quote: ... 3 µl of the microtubule sample was deposited at the surface of a glow-discharged holey carbon grid (Quantifoil R2/2, Cu200) in the temperature (35 °C ...
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bioRxiv - Molecular Biology 2023Quote: 3 µl of freshly purified NbE-µOR-Fab module complex were applied onto holey gold grids (Quantifoil UltrAuFoil R1.2/1.3, 300 mesh), front blotted for 3-4 seconds with 1 mm additional movement (95% humidity at 15°C ...
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bioRxiv - Biophysics 2023Quote: ... 3 μl of medium containing one isolated muscle fibre was placed on the glow-discharged grid (Quantifoil R2/1 Au 200 mesh). After incubation for 60 seconds at 37 °C ...
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bioRxiv - Biophysics 2021Quote: ... 2.8 ul of the mixture was applied onto glow discharged (5 mA, 20 sec) R1.2/1.3 holey carbon gold grids (300 mesh, Quantifoil) at 22°C and 100% humidity ...
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bioRxiv - Microbiology 2022Quote: ... the amoebae (5×105 per well) were seeded onto EM gold finder grids (Au NH2 R2/2, Quantifoil) and incubated for 1 h ...
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bioRxiv - Microbiology 2023Quote: Four microliters of purified TilA-TslA-TlaA1-TlaA2 complex at 0.5 mg ml-1 in Buffer B (Supplementary Table 5) was applied onto glow-discharged (60 s, 30 mA) 300 mesh R1.2/1.3 Au grids (Quantifoil). Grids were blotted for 3 s with blot force of +15 at 10°C and 100% humidity ...
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bioRxiv - Microbiology 2023Quote: ... 2.2µl of the purified mixture at a concentration of 0.5 mg/mL was applied to glow discharged (30 s at 25mA) grid (300 mesh 1.2/1.3, Quantifoil). The grids were blotted for 3.5 s before plunging into liquid ethane using Vitrobot MK4 (TFS ...
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bioRxiv - Biochemistry 2023Quote: A 3.5 μL concentrated SPARTA sample was loaded onto a glow discharged UltrAuFoil 300 mesh R1.2/1.3 grids (Quantifoil) using a FEI Vitrobot at 4°C and 100% humidity ...
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bioRxiv - Neuroscience 2024Quote: ... Plates were shaken at 1,200 rpm for 5 mins at 30°C using a plate shaker (Quantifoil Instruments) and absorption of 405 nm light measured using a FlexStation (Molecular Devices).
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bioRxiv - Biophysics 2024Quote: ... 10 mM DTT was incubated for 3 days at 30 °C and 2.5 μL of the solution was applied onto a Quantifoil R 1.2/1.3 200 mesh Cu grid (Quantifoil) and plunge-frozen in liquid ethane by a Vitrobot Mark IV (FEI) ...
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bioRxiv - Biophysics 2021Quote: ... Log phase cultures were centrifuged at 2000 g for 2 minutes to concentrate the cells 10 times and 4 μl was directly applied to a glow-discharged holey carbon R2/1 Cu 200 mesh grid (Quantifoil). Plunge-freezing was performed using a VitroBot Mark 4 (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: 3.5 μl of protein samples at 4-6 mg/ml were applied to glow-discharged QF R1.2/1.3 300 mesh gold grids (Quantifoil, Großlöbichau, Germany). Grids were blotted for 3 s at 4°C and 100% humidity and plunge-frozen into liquid ethane using FEI Mark IV Vitrobot (FEI ...
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bioRxiv - Plant Biology 2022Quote: ... then 4 µl of the sample was applied to glow-discharged holey carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh). The grid was blotted for 4s and then plunged into liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Cell Biology 2024Quote: ... 4 μl of the cell suspension was applied onto a glow-discharged holey SiO2 grid (R1/4, Au, 200 mesh, Quantifoil) mounted on in the plunger chamber ...
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bioRxiv - Microbiology 2023Quote: ... trehalose solution to mitigate crystalline ice formation and 4 μL of this mixture immediately applied to a R2/1 Cu 200 grid (Quantifoil), which had been glow-discharged for 1 min at 0.19 mbar and 20 mA in a PELCO easiGlow device shortly before use ...
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bioRxiv - Biochemistry 2023Quote: ... pristine graphene-coated R2/4 grids (Graphenea) were initially used for optimisation purposes but 2 nm formvar/amorphous carbon R2/2 grids (Quantifoil) were used for data collection ...
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bioRxiv - Molecular Biology 2023Quote: 1.8 × 105 A549 or A549-IFITM3 cells were seeded on glow-discharged 200 mesh gold grids with a holey SiO2 film with R1/4 spacing (Quantifoil) placed in a 35 mm dish and cultured in 2 ml complete growth medium (DMEM-F12 ...
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bioRxiv - Biophysics 2023Quote: ... 4 µL fresh sample (0.48 mg/mL) was applied on glow-discharged Au holey carbon grids (Quantifoil R 0.6/1) and the following operation were the same as describe above for preparing the sample of gMCM8/9 complex.
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bioRxiv - Biochemistry 2023Quote: ... 4 μL of Fcμ–J–CD5L was applied to glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3, 300 mesh), blotted with filter paper in 100% humidity at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: Cells were seeded and grown overnight on Quantifoil carbon film gold grids (Quantifoil R 1/4 on 200 gold mesh) coated with 10 µg/ml FN ...
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bioRxiv - Biophysics 2024Quote: ... Microtubules (4 µl drop of 40 µM dilution) were placed onto a glow-discharged holey carbon grid (R2/1; Quantifoil) prepared on a JEC-3000FC (JEOL) ...
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bioRxiv - Cell Biology 2024Quote: ... A total of 4 µL of cell suspension (1500–2000 cells/µL) were applied to holey carbon R2/1 or R1/4 copper TEM grids (Quantifoil), and cells were plunge frozen on a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... 3 μL of the purified MC1R-Gs complex was applied to a glow-discharged holey carbon EM grid (Quantifoil, Au 300 R1.2/1.3) in a Vitrobot chamber (FEI Vitrobot Mark IV) ...
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bioRxiv - Biochemistry 2020Quote: ... aliquots (3 μL) of the purified protein concentrated to ~ 10 mg/mL were placed on glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3). For KCC3-DIOA samples ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of the sample was applied to a glow-discharged R2/1 300 mesh holey carbon copper grid (Quantifoil Micro Tools GmbH) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of diluted sample was applied to a glow-discharged (R2/1 300 mesh holey carbon copper grid (Quantifoil Micro Tools GmbH) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Immunology 2022Quote: ... detergent at a final concentration of 0.005 mM was added to each sample and 3 µl of that solution was immediately loaded onto plasma-cleaned QuantiFoil R 2/1 (400-mesh; Quantifoil Micro Tools GmbH) and UltrAuFoil R 1.2/1.3 grids (300-mesh ...
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bioRxiv - Biochemistry 2020Quote: ... 3 μl aliquots of purified mitoribsomes was incubated for 30 s on glow-discharged holey carbon grids (Quantifoil R2/2, 300 mesh, copper) pre-coated with a home-made continuous carbon film with thickness of ~ 27 Å ...
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bioRxiv - Immunology 2020Quote: ... and 3 µl was immediately loaded onto plasma cleaned Quantifoil R 2/1 holey carbon copper gri (Cu, 400-mesh, Quantifoil Micro Tools GmbH). Blotted grids were plunge-frozen into nitrogen-cooled liquid ethane.
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bioRxiv - Biochemistry 2022Quote: ... Peak fractions were collected and 3 μl of TSEN/pre-tRNA complex were applied onto freshly glow-discharged copper grids (Quantifoil, Cu R1.2/1.3) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... 2–3 μL of sample solution was applied to a holey carbon grid (Quantifoil R2/1, Mo 300 mesh; Quantifoil Micro Tools GmbH) covered with a thin amorphous carbon film at 4 °C with 100% humidity ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μL of sample (10 μM EGFP in PBS) was deposited onto glow-discharged grids (Electron Microscopy Science and Quantifoil – see Table 1), prior to blotting the grids from the sample-side for 3 seconds.
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bioRxiv - Molecular Biology 2023Quote: ... A 2.5-3 μL aliquot of protein sample was dropped onto the glow discharged holey carbon grids (Quantifoil Au R1.2/1.3, 300 mesh), blotted with a Vitrobot Mark IV (ThemoFisher Scientific ...