Labshake search
Citations for Quantifoil Micro Tools :
101 - 150 of 219 citations for pIEXBac c EGFP 3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2023Quote: ... 3 μL HA trimer (0.75 mg/ml) was applied to holey carbon grid (Quantifoil 400 mesh Cu R1.2/1.3) which was glow discharged 30 s at 15 mA ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... All samples were pipetted at a volume of 3 µL onto UltrAufoil R1.2/1.3 300 mesh holey grids (Quantifoil, Germany) which had been glow discharged in air at 15 mA for 180 s using a Pelco EasyGlow ...
-
bioRxiv - Biophysics 2022Quote: ... the pelleted cells resuspended in 100 μl NaH2PO4-NaOH pH 7.5 and a 3 μl drop adsorbed for 20 s to a carbon-coated copper grid (300 mesh, Quantifoil) that had been glow-discharged for 30 s at a current of 25 mA ...
-
bioRxiv - Microbiology 2022Quote: ... Purified BfpD was diluted to 4.5 mg/ml and 3 µl was applied to a glow-discharged 300 mesh UltraAufoil -1.2/1.3 holey-gold (Quantifoil, Germany). Grids were blotted for 2 s with ash-free Whatman® Grade 540 filter paper in a Vitrobot Mark IV (ThermoFisher Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... 3 µL of sample were applied on a glow-discharged holey carbon grids (Quantifoil Cu R1.2/1.3 300 mesh), blotted for 5 s at 100% humidity and 4°C and plunge-frozen in liquid ethane using a Vitrobot Mark IV ...
-
bioRxiv - Bioengineering 2024Quote: About 3 μl droplets emulsion were applied onto glow-discharged holey carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh), blotted with a Vitrobot Marker IV (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2024Quote: ... 3 μL of the mixture was applied to a glow-discharged carbon-coated grid (Quantifoil R1.2/1.3 Au300 mesh) and incubated for 60 s in the chamber of a Vitrobot (22°C ...
-
bioRxiv - Biophysics 2024Quote: ... The sample was applied in aliquots of 3 µL to Quantifoil R 1.2/1.3 holey carbon (copper) grids (Quantifoil microtools), which were rendered hydrophilic before use by glow-discharge treatment (Pelco easiGlow) ...
-
bioRxiv - Cell Biology 2024Quote: 3 µl cell suspension was applied on glow-discharged (Pelco easiGlow) R1/4 SiO2 gold 200 mesh grids (Quantifoil), blotted for 10 sec from the backside and plunge-frozen on a Leica EM GP2 plunger at 23 °C ...
-
bioRxiv - Biophysics 2020Quote: Three microliters of purified HCoV-NL63 virions (4.5 mg/mL) were first applied to 300-mesh R2/1 + 2 nm C-film grids (Quantifoil) that had been glow-discharged for 15s PELCO easiGlow (TED PELLA ...
-
bioRxiv - Biophysics 2024Quote: ... Aliquots of 1.25 – 1.5 ul RyR1-nanodisc were applied onto each side of glow-discharged C-Flat - 2/1 - 3Au holey-carbon (Protochips, NC) or UltraAufoil -1.2/1.3 holey-gold (Quantifoil, Germany) with 300 TEM mesh ...
-
bioRxiv - Immunology 2023Quote: ... The plate was then mixed (200rpm; 37°C) for 20 minutes in the dark (BioShake iQ, Quantifoil Instruments GmbH). Samples were fixed with 1% formalin and run on the Cytek Aurora 5-laser flow cytometer (Cytek Biosciences) ...
-
bioRxiv - Cell Biology 2022Quote: Cells were trypsinized and seeded onto 200 mesh gold holey carbon grids (R2/2-2 C, Quantifoil Micro Tools) placed in 3D printed grid holders (51 ...
-
bioRxiv - Molecular Biology 2020Quote: ... at 13 °C and 100% humidity: 3 μl of sample were applied onto a freshly glow-discharged copper R2/1 300 mesh grid (Quantifoil), blotted for 8 s on both sides with blotting force −20 and plunge-frozen in liquid ethane.
-
bioRxiv - Biophysics 2020Quote: ... and urine were applied in 3 µl-volumes onto glow-discharged Quantifoil Au R2/2 holey carbon 300 mesh grids (Quantifoil). Grids were blotted for 2.0 s and plunged into liquid ethane cooled by liquid nitrogen ...
-
bioRxiv - Biochemistry 2020Quote: ... A volume of 3 μL of the sample was applied to a glow-discharged grid (Quantifoil R2/1 300 mesh) at 25 °C and 90 % humidity ...
-
bioRxiv - Microbiology 2021Quote: ... 3 μL were applied onto a Cu grid with a holey carbon film (Quantifoil R 2/2; Quantifoil, Jena, Germany), blotted ...
-
bioRxiv - Microbiology 2021Quote: ... 3 μL were applied onto a Cu grid with a holey carbon film (Quantifoil R 2/2; Quantifoil, Jena, Germany), blotted ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: Samples (3 μL) were applied to a glow-discharged UltrAufoil R1.2/1.3 300 mesh holey grid (Quantifoil GmbH, Großlöbichau, Germany) and were flash frozen in liquid ethane using the Vitrobot mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... 3 µl of the samples at 1 µg/µl was applied to UltrAUfoil R1.2/1.3 grids (Quantifoil Micro Tools GmbH). Grids were glow-discharged for 30 sec at 15 mA prior to sample application (PELCO easiGlow™ ...
-
bioRxiv - Microbiology 2022Quote: ... 3 mm gold EM finder grids with a perforated carbon film (R 2/2, 200 mesh) were purchased from Quantifoil (AU G200F1 finder ...
-
bioRxiv - Biochemistry 2022Quote: 2.8 µl of F-actin sample (3 – 16 µM) was pipetted onto a glow-discharged R2/1 Cu 300 mesh holey-carbon grid (Quantifoil). After incubating for 1 – 2 seconds ...
-
bioRxiv - Immunology 2022Quote: ... 3 μL of the mixture was applied to a glow-discharge holey carbon grids (Quantifoil, Cu 200 mesh, R1.2/1.3). The grid was blotted for 4.5 s in 100% humidity before being flash frozen in liquid ethane by using a Vitrobot Mark IV (Thermo Fisher) ...
-
bioRxiv - Molecular Biology 2020Quote: 3.5 µl of the concentrated sample were applied to glow discharged copper grids with holey carbon support and a 2 nm continuous carbon layer (R3/3, Quantifoil). Grids were blotted in a Vitrobot Mark IV (FEI Company ...
-
bioRxiv - Microbiology 2021Quote: ... An aliquot (3 μl) of this mixture was applied to a glow-discharged holey carbon grid (QF R2/2 Cu, QUANTIFOIL), blotted with Whatman® No ...
-
bioRxiv - Cell Biology 2023Quote: ... An aliquot of 3 ul cell suspension was applied to the glow-discharged holey carbon-coated copper (R 2/1, 200 mesh) (Quantifoil) and blotted for 9 seconds by Leica GP2 (Leica Microsystems) ...
-
bioRxiv - Immunology 2023Quote: ... 3 μL of the purified complex were loaded onto a glow-discharged holey grid (Quantifoil Au 300 mesh R1.2/1.3), and subsequently were plunge-frozen in liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2024Quote: ... 3 µl of cell suspension was applied to a freshly grown discharged holey carbon grid (Quantifoil® #200, R2/2). Grids were immediately blotted from the back side using Whatman filter paper #1 (GE Healthcare ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... 3 μl of the samples were applied to glow-discharged holey carbon EM grids (Quantifoil 300 copper mesh, R1.2/1.3) in an EM-GP2 plunge freezer (Leica) ...
-
bioRxiv - Immunology 2023Quote: ... 3 μL of each LNP sample was deposited onto a holey carbon grid that was glow-discharged (Quantifoil R1.2/1.3) and vitrificated using a Vitrobot Mark IV System (FEI/Thermo Scientific ...
-
bioRxiv - Biochemistry 2023Quote: ... 3 µL of each sample was applied to glow-discharged (PELCO easiGlow, 15 mA, 2 min) holey carbon grid (Quantifoil R1.2/1.3 on gold or copper 200 mesh support) ...
-
bioRxiv - Biochemistry 2024Quote: ... 3 μL of hexapeptide sample were applied to glow-discharged Quantifoil R 2/2 grids (Quantifoil Micro tools GmbH, Germany), blotted for 1 second ...
-
bioRxiv - Neuroscience 2024Quote: Plunge-freezing VLPs: 3 µL of crude enriched VLP resuspension was applied to Holey copper R2/2 grids (Quantifoil GmBH) glow-discharged at 5mA 30s using an EasiGlow system (Pelco) ...
-
bioRxiv - Neuroscience 2024Quote: Protein sample aliquots of 3 µL were applied to glow-discharged holey carbon grids (Quantifoil Au R1.2/R1.3 300 mesh), blotted with filter paper ...
-
bioRxiv - Biophysics 2024Quote: ... Cryo-EM grids were obtained by flash-freezing 3 µL of purified IPNV particles on glow-discharged holey carbon grids (Quantifoil R2/2 ...
-
bioRxiv - Cell Biology 2021Quote: ... Aggregation was stimulated with TRAP-6 (25μM) in a 96-well plate (15 minutes, 750rpm, 37°C; BioShake IQ, Quantifoil Instruments) and fixed with 1% formalin (Sigma) ...
-
bioRxiv - Biochemistry 2022Quote: ... 3.0 μL of sample was applied to glow-discharged 200-mesh copper 2 nm C R1.2/1.3 cryo-EM grids (Quantifoil Micro Tools), blotted 1 s at 100 % humidity ...
-
bioRxiv - Biochemistry 2022Quote: ... 2.5 μL of sample was applied to glow-discharged 200-mesh copper 2 nm C R1.2/1.3 cryo-EM grids (Quantifoil Micro Tools), blotted 3 s at 75 % humidity ...
-
bioRxiv - Biochemistry 2021Quote: ... 3.5 μL sample was applied onto a freshly glow-discharged 200-mesh copper 2 nm C R1.2/1.3 grids (Quantifoil Micro Tools). The grids were blotted for 1 s and plunge-vitrified in liquid ethane ...
-
bioRxiv - Biochemistry 2023Quote: 2.4 µL of sample were applied to a freshly glow discharged 200-mesh Cu + 2 nm C R1.2/1.3 grid (Quantifoil Micro Tools), blotted for 6 s at 100% humidity ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... The proteins were incubated at 37°C and 200 rpms for 10 minutes on a microplate shaker (BioShake iQ; Quantifoil Instruments ...
-
A distinctive ligand recognition mechanism by the human vasoactive intestinal polypeptide receptor 2bioRxiv - Molecular Biology 2021Quote: The purified PACAP27–VIP2R–Gs complex (3 μL at 3.7 mg per mL) was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3). Vitrification was performed using a Vitrobot Mark IV (ThermoFisher Scientific ...
-
bioRxiv - Biochemistry 2020Quote: ... the grids were prepared by applying 3 μl sample on glow-discharged holey carbon grids (Quantifoil Mo 2/2 200 mesh). The sample was ice-embedded using a LEICA EM GP2 (Leica Microsystems ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: Samples (3 µL) were applied to a glow-discharged UltrAuFoil R1.2/1.3 Au 300 gold foil grids (Quantifoil GmbH, Großlöbichau, Germany) and were flash frozen in liquid ethane using a Vitrobot mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Biophysics 2020Quote: ... samples were stored on ice and applied to the cryo-grids (2 nm precoated Quantifoil R3/3 holey carbon supported grids) at a concentration of 10 μM and vitrified using a Vitrobot Mark IV (FEI) ...
-
Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... 3 µL samples were applied to freshly glow-discharged Cu 200 Holey Carbon R1.2/1.3 grids (Quantifoil, for samples without FFosC), or Cu 200 Holey Carbon R2/1 grids (Quantifoil ...
-
bioRxiv - Microbiology 2020Quote: ... 3 μL of the sample mixture was applied to a freshly glow-discharged copper R2/2 200 grid (Quantifoil Micro Tools), pre-blotted for 30 seconds ...
-
bioRxiv - Biochemistry 2021Quote: The purified TIP39–PTH2R–Gs complex (3 μL at 8.5 mg per mL) was applied on a glow-discharged holey carbon grid (Quantifoil R1.2/1.3). Vitrification was performed using a Vitrobot Mark IV (ThermoFisher Scientific ...
-
bioRxiv - Biochemistry 2022Quote: ... stock solution was mixed with 3.5 µL of the KWOCA 4 sample and 3 µl were immediately loaded onto the UltrAuFoil R 1.2/1.3 grids (Au, 300-mesh; Quantifoil Micro Tools GmbH). Prior to sample loading the grids were subjected to Ar/O2 plasma cleaning for 10 s on a Solarus 950 plasma cleaner (Gatan) ...
-
bioRxiv - Biophysics 2024Quote: Approximately 3□μL of the sample solution were put on newly glow-discharged TEM grids (Quantifoil R2/1, Cu, mesh 200) and plunge-frozen in liquid ethane using the Vitrobot Mark IV (Thermo Fisher Scientific) ...