Labshake search
Citations for Quantifoil Micro Tools :
101 - 150 of 193 citations for R + SCH 23390 hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... These samples were applied onto UltrAuFoil R 1.2/1.3 grids (Au, 300-mesh; Quantifoil Micro Tools GmbH). Prior to sample application the grids were glow-discharged using Ar/O2 plasma for 10 s on a Solarus 950 plasma cleaner (Gatan) ...
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bioRxiv - Biochemistry 2021Quote: ... 3 microlitres of undiluted eluate and of eluate which was diluted 1:2 or 1:4 in buffer A200 were blotted onto holey carbon R 1.2/1.3 copper 300 mesh grids (Quantifoil) and vitrified in liquid ethane using a Vitrobot MarkIV device (Thermofisher) ...
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bioRxiv - Molecular Biology 2022Quote: Purified SARS-CoV-2 spike samples in 10mM Hepes pH7.2 and 150mM NaCl (3 μL at 0.62-0.75mg/ml) were kept at 4C until their application onto QUANTIFOIL R 1.2/1.3 Cu:300-mesh grids (QUANTIFOIL). The grids were vitrified using a Leica GP automatic vitrification robot (Leica) ...
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bioRxiv - Biochemistry 2022Quote: ... 2 μl retromer at a concentration between 80-100 μg/ml was applied to freshly glow discharged CF-2/2-2C C-Flat grids (Protochips, Morrisville, NC) or Quantifoil R 1.2/1.3 300 mesh copper grids (Quantifoil Micro Tools GmbH ...
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bioRxiv - Biochemistry 2022Quote: ... 3 μl of sample was applied to a plasma treated gold coated R 1.2/1.3 300 mesh holey carbon grid (Quantifoil), with a blot force of 0 ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μl of protein sample was applied to holey carbon grids (Quantifoil, R 0.6/1 Cu 200 mesh) which were H2/O2 glow-discharged for 30 s ...
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bioRxiv - Cell Biology 2020Quote: ... 2.5 μl of samples was frozen onto a glow discharged Quantifoil Gold R 1.2/1.3 300 mesh grid (Quantifoil). Samples were frozen under 100% humidity ...
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bioRxiv - Biochemistry 2021Quote: ... a 5 μl drop was applied to a graphene-treated and glow-discharged holey carbon grid (R 1.2/1.3 Quantifoil) for the BsClpP pH 4.2 sample and negatively glow-discharged holey carbon grid (R 1.2/1.3 Quantifoil ...
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bioRxiv - Biophysics 2021Quote: ... 3 μl sample was applied to each side of the UltrAuFoil grid (300 mesh, R 1.2/1.3, Quantifoil), which had been glow discharged on both sides (25 mA ...
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bioRxiv - Immunology 2022Quote: ... 3 μL of the DCFHP proteins were applied onto a glow-discharged Quantifoil R 1.2/1.3 Cu 300-mesh grid (Quantifoil). The grids were blotted for 2 sec at 20°C and 100% humidity and rapidly cryocooled in liquid ethane using a Vitrobot Mark IV instrument (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... 3μL of protein was deposited on holey carbon copper grids (QUANTIFOIL R 1.2/1.3-2nm, 300 mesh, EMS) which had been glow-discharged for 20s at 15 mA using PELCO easiGlow (TedPella Inc) ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μl of sample was applied to a plasma treated gold coated R 1.2/1.3 300 mesh holey carbon grid (Quantifoil), with a blot force of 0 ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 ul aliquot of the mixture was adsorbed onto a glow-discharged Quantifoil R 1.2/1.3 300 mesh grid (Quantifoil) and vitrified in liquid ethane with a Leica EM GP2 cryoplunger (Leica ...
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bioRxiv - Biochemistry 2024Quote: ... 3.2 μl samples of the solution were applied onto EM grids (Quantifoil, R 1.2/1.3 Cu 300 mesh) that had been glow discharged with PELCO easiGlow for 60 seconds in Vitrobot Mark IV chamber at 4°C and 90% humidity ...
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bioRxiv - Microbiology 2024Quote: ... was applied to previously glow discharged R 1.2/1.3 Molybdenum 200 grids with a holey carbon support film (Quantifoil). Grids were quickly transferred to an EM GP Leica for blotting for 1.5-2.5 seconds at 90% humidity and flash frozen in liquid ethane cooled by liquid nitrogen ...
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bioRxiv - Biophysics 2024Quote: ... 10 mM DTT was incubated for 3 days at 30 °C and 2.5 μL of the solution was applied onto a Quantifoil R 1.2/1.3 200 mesh Cu grid (Quantifoil) and plunge-frozen in liquid ethane by a Vitrobot Mark IV (FEI) ...
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bioRxiv - Microbiology 2021Quote: ... and 5 µl of the mixture was placed onto freshly glow discharged holey carbon grids (R 2/1, Quantifoil) for 1 min ...
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bioRxiv - Molecular Biology 2021Quote: Purified mtLSU or 55S mitoribosome samples (4 μL) were applied to freshly glow discharged R 3.5/1 holey carbon grids (Quantifoil) that were precoated with a 2-3 nm carbon layer using a Leica EM ACE600 coater ...
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bioRxiv - Microbiology 2021Quote: An aliquot (2.5 µL) of the purified tokyovirus particles was placed onto R 1.2/1.3 Quantifoil grids (Quantifoil Micro Tools) that were glow-discharged using a plasma ion bombarder (PIB-10 ...
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bioRxiv - Bioengineering 2023Quote: ... a 200-mesh copper grid covered with a Quantifoil R 2/2 holey carbon film (Quantifoil Micro Tools GmbH) was surface plasma treated for 40 seconds using a Cressington 208 carbon coater ...
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bioRxiv - Biophysics 2023Quote: ... using FluoSpheres (diameter of 1 μm) on gold Quantifoil grids (200 mesh, R 2/1, holey SiO2 film; Quantifoil Micro Tools GmbH ...
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bioRxiv - Biophysics 2023Quote: ... glow discharged QUANTIFOIL® copper R 2/2 grid with a 2 nm thin extra carbon layer (QUANTIFOIL, GMBH) was used ...
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bioRxiv - Cell Biology 2023Quote: ... Two μl of the diluted complex were applied to freshly glow-discharged R 2/2 holey carbon grids (Quantifoil), which were blotted with a force of 6 for 5 seconds and immediately vitrified by plunge-freezing in liquid ethane using a Vitrobot Mark IV (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... Double application of 3 μL protein (∼0.6 mg/mL) SYM2081 complex was carried out on glow-discharged gold grids (R 0.6/1, 300 mesh, Quantifoil). The grids were blotted for 4.5 s and 3.5 s ...
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bioRxiv - Microbiology 2024Quote: ... after applying 2.5 µl of sample to a cryoEM grid (Quantifoil Cu/Rh R3.5/1, UltrAuFoil R 1.2/1.3) as described previously56.
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bioRxiv - Biophysics 2024Quote: ... The sample was applied in aliquots of 3 µL to Quantifoil R 1.2/1.3 holey carbon (copper) grids (Quantifoil microtools), which were rendered hydrophilic before use by glow-discharge treatment (Pelco easiGlow) ...
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bioRxiv - Biochemistry 2020Quote: ... 3 μL of the concentrated RdRp-RNA complex were mixed with 0.5 μl of octyl ß-D-glucopyranoside (0.003% final concentration) and applied to freshly glow discharged R 2/1 holey carbon grids (Quantifoil). The grids were blotted for 5 seconds using a Vitrobot MarkIV (Thermo Fischer Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... 3 µL of the concentrated RdRp-RNA complex were mixed with 0.5 µl of octyl ß-D-glucopyranoside (0.003% final concentration) and applied to freshly glow discharged R 2/1 holey carbon grids (Quantifoil). The grids were blotted for 7 seconds with blot force 5 using a Vitrobot MarkIV (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2021Quote: ... 3 μL were applied onto a Cu grid with a holey carbon film (Quantifoil R 2/2; Quantifoil, Jena, Germany), blotted ...
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bioRxiv - Microbiology 2021Quote: ... 3 μL were applied onto a Cu grid with a holey carbon film (Quantifoil R 2/2; Quantifoil, Jena, Germany), blotted ...
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bioRxiv - Microbiology 2022Quote: ... 3 mm gold EM finder grids with a perforated carbon film (R 2/2, 200 mesh) were purchased from Quantifoil (AU G200F1 finder ...
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bioRxiv - Microbiology 2021Quote: ... Aliquot (2.5 μL) of the sample was placed onto a holey-carbon copper grid (Quantifoil Micro Tools, R 1.2/1.3) previously glow-discharged in a plasma ion bombarder (PIB-10 ...
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bioRxiv - Biophysics 2020Quote: ... 3 μL of the concentrated RdRp-RNA complex were mixed with 0.5 μl of octyl ß-D-glucopyranoside (0.003% final concentration) and applied to freshly glow discharged R 2/1 holey carbon grids (Quantifoil). Prior to flash freezing in liquid ethane ...
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High-resolution model of Arabidopsis Photosystem II reveals the consequences of digitonin-extractionbioRxiv - Plant Biology 2021Quote: ... 4 μl concentrated sample was applied to glow-discharged (30 s at 50 mA) Quantifoil R 1.2/1.3 Cu 300 grids (Quantifoil Micro Tools) and grids were plunge frozen in liquid ethane using an FEI Vitrobot MkIV (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... 105 fluorescence-stained cells were deposited on the surface of Au-G200F1 finder grids coated with holey carbon (R 2/2; Quantifoil) and functionalized with Poly-L-Lysine (Merck ...
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bioRxiv - Biophysics 2020Quote: ... Mycoplasma pneumoniae strain M129(ATCC 29342)cells were grown on 200 mesh gold grids coated with a holey carbon support (R 2/1, Quantifoil). Cells were cultivated at 37 °C in modified Hayflick medium ...
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bioRxiv - Microbiology 2022Quote: ... mixed with 10 nm protein A-coated colloidal gold (Aurion) and applied onto a glow-discharged EM grid (200 mesh, R 2/1, Quantifoil) prior to plunge-freezing with a Leica EM GP2 automatic plunge-freezer.
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bioRxiv - Biochemistry 2024Quote: ... Vitrified specimen was prepared by applying 3 μl of protein complexes onto a glow-discharged 300 mesh gold holey carbon Quantifoil R 1.2/1.3 grid (Quantifoil Micro Tools) and frozen in liquid ethane cooled by liquid nitrogen inside a Vitrobot Mark IV (FEI) ...
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bioRxiv - Cell Biology 2023Quote: Cells were blotted on EM grids (R 2/1 holey carbon 200 films on Cu 200 mesh, Quantifoil Micro Tools). The grids were first glow-discharged and 4 µl of trypanosome wild-type culture (2-4 · 107 cells/ml ...
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bioRxiv - Cell Biology 2023Quote: ... An aliquot of 3 ul cell suspension was applied to the glow-discharged holey carbon-coated copper (R 2/1, 200 mesh) (Quantifoil) and blotted for 9 seconds by Leica GP2 (Leica Microsystems) ...
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bioRxiv - Biophysics 2023Quote: ... HeLa cells were trypsinized and seeded at a density of 0.5 – 0.7 X 105 cells/mL on carbon-coated gold Quantifoil grid (200 mesh, R 2/1, holey SiO2 film; Quantifoil Micro Tools GmbH ...
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bioRxiv - Biophysics 2023Quote: A 3 µL aliquot of purified CDTbD623A/D734Aprotein sample at a concentration of 30 µM was applied to a glow discharged QUANTIFOIL® copper R 1.2/1.3 grid with a 2 nm thin extra carbon layer (QUANTIFOIL, GMBH). For calcium depleted CDTb (28 µM) ...
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bioRxiv - Biophysics 2023Quote: ... 4 µL fresh sample (0.48 mg/mL) was applied on glow-discharged Au holey carbon grids (Quantifoil R 0.6/1) and the following operation were the same as describe above for preparing the sample of gMCM8/9 complex.
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bioRxiv - Microbiology 2023Quote: ... 2 μL of sample was applied on each side of a freshly glow-discharged QUANTIFOIL R 1.2/1.3 Cu 200-mesh grid (Quantifoil Micro Tools). The grid was blotted at once for 15 s from the back side and plunged into liquid ethane using EM GP2 (Leica Microsystems ...
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bioRxiv - Cell Biology 2023Quote: ... 150 or 200 mesh gold holey carbon grids (R 2/2, #N1-C16nAu15-01 and #N1-C16nAu20-01, respectively) were purchased from Quantifoil Micro Tools and glow discharged for 2 min in an ELMO glow discharge unit (Agar Scientific ...
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bioRxiv - Cell Biology 2024Quote: Cells were seeded and grown overnight on Quantifoil carbon film gold grids (Quantifoil R 1/4 on 200 gold mesh) coated with 10 µg/ml FN ...
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bioRxiv - Biochemistry 2024Quote: ... 3 μL of hexapeptide sample were applied to glow-discharged Quantifoil R 2/2 grids (Quantifoil Micro tools GmbH, Germany), blotted for 1 second ...
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bioRxiv - Plant Biology 2020Quote: ... 4 μL of cell culture was blotted onto R 1/4 SiO2-coated 200-mesh gold EM grids (Quantifoil Micro Tools) and plunge frozen in a liquid ethane/propane mixture ...
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bioRxiv - Microbiology 2021Quote: ... 3μl of protein solution at a concentration of 0.2 mg/ml were deposited on glow-discharged Quantifoil R 1.2/1.3 holey carbon grids (Quantifoil Micro Tools GmbH, Germany). Samples were manually blotted for four seconds at room temperature and vitrified by rapidly plunging into liquid ethane using a home-built plunging apparatus ...
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bioRxiv - Biophysics 2022Quote: ... 10 µl sample was applied onto a glow discharged copper grid coated with holey carbon (R 2/2; Quantifoil, Jena, Germany), and subsequently dipped onto 500 µl deionized H2O for 1 second to clear the sucrose ...