Labshake search
Citations for Quantifoil Micro Tools :
101 - 150 of 658 citations for L β Homo β 2 hydroxyphenyl glycine; R 3 Amino 3 2 hydroxyphenyl propanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Holey copper or molybdenum EM grids (Quantifoil R 2/1 200 mesh; Quantifoil, Jena, Germany) were glow discharged (Harrick Plasma cleaner PDC-3XG ...
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bioRxiv - Biochemistry 2024Quote: ... 3.5 µL were applied to freshly glow-discharged R 2/1 holey carbon grids (Quantifoil) at 4 °C and 95% humidity ...
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bioRxiv - Biophysics 2024Quote: ... The sample was applied in aliquots of 3 µL to Quantifoil R 1.2/1.3 holey carbon (copper) grids (Quantifoil microtools), which were rendered hydrophilic before use by glow-discharge treatment (Pelco easiGlow) ...
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bioRxiv - Molecular Biology 2021Quote: ... 3.5 μL was applied to a glow-discharged Quantifoil copper grid with 2 nm continuous carbon support over holes (Quantifoil R 2/4 400 mesh), blotted ...
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bioRxiv - Biophysics 2021Quote: ... of purified CtenDNAV-II virons (10 mg ml-1) was deposited onto freshly glow-discharged holey carbon-coated copper grids (Quantifoil R 2/2, 300 mesh, copper) followed by 2 s of blotting in 100% relative humidity for plunge-freezing (Vitrobot Mark IV ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were prepared by depositing 3 μl of the crystalline slurry onto a glow-discharged EM grid (Quantifoil, Cu 200 mesh, R2/2 holey carbon). Excess liquid was blotted away and the sample was vitrified using a Leica GP2 vitrification robot ...
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bioRxiv - Microbiology 2020Quote: ... 3.5 μl of that mixture was transferred to a 3 mm freshly glow-discharged Quantifoil R2/2 Cu 300 mesh holey carbon grid (Quantifoil Micro Tools GmbH, Jena, Germany). Excess liquid was blotted away using a Leica EM GP plunge-freezer at room temperature and 80% humidity (blot time 2-3 sec ...
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bioRxiv - Molecular Biology 2022Quote: ... The pellet was then resuspended in warm EM buffer and 3 μL of this sample was applied to a carbon on gold grid (Quantifoil R2/2 Au 200 mesh), recently glow-discharged for 1 min at 30 mA ...
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bioRxiv - Immunology 2021Quote: ... was loaded onto plasma-cleaned Quantifoil R 2/1 (Cu, 400-mesh; Quantifoil Micro Tools GmbH) grids ...
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bioRxiv - Biochemistry 2024Quote: 4 μl of the inhibition reaction mixture was applied onto plasma cleaned holey carbon grids coated with 2nm thick amorphous carbon (Quantifoil R 2/2, UT, 300 Mesh, Copper). Following 30 sec of incubation inside the chamber of FEI Vitrobot MK IV (FEI ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µl of sample were applied to glow-discharged Quantifoil Au 2/2 holey carbon 200 mesh grids or Ultrafoil Au 2/2 holey gold 200 mesh grids (Quantifoil), and flash frozen in liquid ethane using an FEI Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... stock solution was mixed with 3.5 µL of the KWOCA 4 sample and 3 µl were immediately loaded onto the UltrAuFoil R 1.2/1.3 grids (Au, 300-mesh; Quantifoil Micro Tools GmbH). Prior to sample loading the grids were subjected to Ar/O2 plasma cleaning for 10 s on a Solarus 950 plasma cleaner (Gatan) ...
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bioRxiv - Biochemistry 2020Quote: UltrAUfoil 2/2 300 mesh gold grids (Quantifoil) were used for open hole data collection (Dataset 1) ...
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bioRxiv - Cell Biology 2020Quote: ... and seeded onto a glow discharged holey carbon covered EM-grids (R 2/1, Au 200, Quantifoil). After approximately 16 h ...
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bioRxiv - Biochemistry 2024Quote: ... Those nucleosomes that passed the quality control were then loaded into a freshly glow discharged holey carbon golden grid (Quantifoil™ R 2/2 on 300 gold mesh) (2.5 µg of nucleosome diluted in 40 mM NaCl reconstitution buffer in 3 μl was loaded in the grid) ...
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bioRxiv - Biochemistry 2023Quote: ... A 3 µL drop was applied to a glow-discharged (PELCO easiGlow, 15 mA, 2 min) holey carbon grid (Quantifoil R1.2/1.3 on gold 200 mesh support), blotted for 3-4 seconds with Whatman Grade 595 filter paper (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... 2 μl retromer at a concentration between 80-100 μg/ml was applied to freshly glow discharged CF-2/2-2C C-Flat grids (Protochips, Morrisville, NC) or Quantifoil R 1.2/1.3 300 mesh copper grids (Quantifoil Micro Tools GmbH ...
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bioRxiv - Biophysics 2022Quote: UltraAUfoil R1.2/3 (Quantifoil Micro Tools GmbH) grids were glow-discharged on both sides for 30 s at 15 mA using a Pelco Easiglow ...
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bioRxiv - Microbiology 2023Quote: ... 3 μL of 3 mg/mL of protein was added to glow discharged UltrAuFoil holey gold grid (Quantifoil GmbH) using a Vitrobot Mark IV (Thermofisher ...
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bioRxiv - Biochemistry 2024Quote: ... was used to apply the purified samples (3.5 µl) to freshly glow discharged R3/3 holy copper grids supported with 3 nm continuous carbon (Quantifoil). The samples were incubated for 45 s on the grids at 4 °C and 95% humidity ...
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bioRxiv - Microbiology 2021Quote: ... and 5 µl of the mixture was placed onto freshly glow discharged holey carbon grids (R 2/1, Quantifoil) for 1 min ...
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bioRxiv - Biophysics 2023Quote: ... using FluoSpheres (diameter of 1 μm) on gold Quantifoil grids (200 mesh, R 2/1, holey SiO2 film; Quantifoil Micro Tools GmbH ...
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bioRxiv - Immunology 2024Quote: ... The sample was prepared on Quantifoil Cu 300 2/2 (Quantifoil) grids and was frozen in liquid ethane using the Vitroblot Mark IV (Thermo Fisher ...
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bioRxiv - Biochemistry 2020Quote: ... 3 μL of the concentrated RdRp-RNA complex were mixed with 0.5 μl of octyl ß-D-glucopyranoside (0.003% final concentration) and applied to freshly glow discharged R 2/1 holey carbon grids (Quantifoil). The grids were blotted for 5 seconds using a Vitrobot MarkIV (Thermo Fischer Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... 3 µL of the concentrated RdRp-RNA complex were mixed with 0.5 µl of octyl ß-D-glucopyranoside (0.003% final concentration) and applied to freshly glow discharged R 2/1 holey carbon grids (Quantifoil). The grids were blotted for 7 seconds with blot force 5 using a Vitrobot MarkIV (Thermo Fischer Scientific ...
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bioRxiv - Biophysics 2020Quote: ... 3 μL of the concentrated RdRp-RNA complex were mixed with 0.5 μl of octyl ß-D-glucopyranoside (0.003% final concentration) and applied to freshly glow discharged R 2/1 holey carbon grids (Quantifoil). Prior to flash freezing in liquid ethane ...
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bioRxiv - Biophysics 2020Quote: ... Mycoplasma pneumoniae strain M129(ATCC 29342)cells were grown on 200 mesh gold grids coated with a holey carbon support (R 2/1, Quantifoil). Cells were cultivated at 37 °C in modified Hayflick medium ...
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bioRxiv - Cell Biology 2023Quote: Cells were blotted on EM grids (R 2/1 holey carbon 200 films on Cu 200 mesh, Quantifoil Micro Tools). The grids were first glow-discharged and 4 µl of trypanosome wild-type culture (2-4 · 107 cells/ml ...
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bioRxiv - Biophysics 2023Quote: ... HeLa cells were trypsinized and seeded at a density of 0.5 – 0.7 X 105 cells/mL on carbon-coated gold Quantifoil grid (200 mesh, R 2/1, holey SiO2 film; Quantifoil Micro Tools GmbH ...
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bioRxiv - Biophysics 2023Quote: A 3 µL aliquot of purified CDTbD623A/D734Aprotein sample at a concentration of 30 µM was applied to a glow discharged QUANTIFOIL® copper R 1.2/1.3 grid with a 2 nm thin extra carbon layer (QUANTIFOIL, GMBH). For calcium depleted CDTb (28 µM) ...
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bioRxiv - Microbiology 2022Quote: ... mixed with 10 nm protein A-coated colloidal gold (Aurion) and applied onto a glow-discharged EM grid (200 mesh, R 2/1, Quantifoil) prior to plunge-freezing with a Leica EM GP2 automatic plunge-freezer.
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bioRxiv - Biophysics 2023Quote: ... 4 μl of 4.3 mg/ml Slo1-γ1 complex were applied to glow-discharged UltrAuFoil R2/2 200 grid (Quantifoil). Any excess liquid was removed by blotting for 3.5 s at a blot force of −3 ...
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bioRxiv - Biochemistry 2022Quote: R2/2 grids (Quantifoil) were covered with a thin layer of continuous carbon (estimated to be 50 Å thick ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... supplemented with 0.05%octyl-β-D-glucopyranoside was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3). The grids were blotted for 2 sec using an FEI Vitrobot Mark IV (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... Glow-discharged carbon coated gold mesh grids (QF 2/2 AU 200, Quantifoil) were placed on the edge of the glass microscopy slides ...
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bioRxiv - Neuroscience 2020Quote: ... covered with either 2 nm amorphous carbon (Quantifoil, R2/1+2 nm C) or graphene were used for sample preparation ...
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bioRxiv - Immunology 2023Quote: ... respectively) and 3 μl were kept 5 min at RT until their application onto glow-discharged holey carbon grids (Quantifoil, Au 300 mesh, R 0.6/1). The grids were blotted and then plunged into liquid ethane using a FEI Vitrobot Mark IV at 20°C and 95% relative humidity ...
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bioRxiv - Biochemistry 2024Quote: 3µl of the AtGDH-AKG bound binary complex (3 mg/ml) was applied to freshly glow-discharged holey carbon grids (Quantifoil Au R 0.6/1.0 300 mesh). For the apo form AnGDH dataset ...
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bioRxiv - Biochemistry 2023Quote: ... the same proteins were applied to holey grids with a 2 nm carbon film (Quantifoil Cu 2/2 200 mesh from EMS). Samples were frozen after a total of 145 min on the grids without carbon film and 150 min on the grids with carbon film ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µL of 10 µM 11-16Ub[K48]-GFP-35 was pipetted on a holey carbon R2/2 Cu200 grid (Quantifoil) that was plasma cleaned by a SCD005 sputter coater (Bal-Tec ...
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bioRxiv - Microbiology 2024Quote: ... Sample volumes of 3.5 µl (8 OD260 per ml) were applied to grids (Quantifoil, Cu, 300 mesh, R3/3 with 3 nm carbon) which had been freshly glow-discharged using a GloQube (Quorum Technologies ...
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bioRxiv - Biochemistry 2022Quote: KRASGTPase/BRAFWT/MEK1SASA/14-3-3 complex in SEC buffer was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, 400 mesh) and vitrified using a Leica EM GP ...
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bioRxiv - Biochemistry 2024Quote: Sample volumes of 3.5 μl (8 OD260 per ml) were applied to grids (Quantifoil, Cu, 300 mesh, R3/3 with 3 nm carbon) which had been freshly glow-discharged using a GloQube (Quorum Technologies ...
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bioRxiv - Cell Biology 2021Quote: Gold grids (Quantifoil, R2/2) were glow-discharged and incubated on top of 40 μL droplets of fibronectin (50 μg/mL ...
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bioRxiv - Biochemistry 2024Quote: ... R2/2 UltrAuFoil grids (Quantifoil) were glow-discharged for 100 s ...
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bioRxiv - Biochemistry 2024Quote: ... R2/2 UltrAuFoil grids (Quantifoil) were glow-discharged for 100 s ...
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bioRxiv - Molecular Biology 2021Quote: Copper grids with holey carbon support film (R3/3, Quantifoil) and a 2 nm pre-coated continuous carbon layer on top were glow discharged at 2.2 × 10-1 mbar for 20 s ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... supplemented with 0.02% octyl-β-D-glucopyranoside was applied to glow-discharged UltrAuFoil holey gold grids (Quantifoil, Au300-R1.2/1.3). GTP was added to the grid at a final concentration of 1mM and the grids were blotted using an FEI Vitrobot Mark IV (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... applied to glow-discharged 2/2 μm holey carbon-coated 200-mesh gold grids (Quantifoil) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... 3.5 μl of cell suspension from 80-100 μl of final volume were applied on glow discharged 200-mesh EM copper grids coated with R 2/1 holey SiO2 films (Quantifoil Micro Tool GmbH) and plunge frozen in liquid ethane at ~−184°C using a Leica EM GP grid plunger ...