Labshake search
Citations for Quantifoil Micro Tools :
701 - 750 of 786 citations for 2 Aminomethyl 1 3 thiazole 4 carboxylic Acid Hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... or Cu 200 Holey Carbon R2/1 grids (Quantifoil, for samples containing 1.5 mM FFosC). In both cases ...
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bioRxiv - Molecular Biology 2022Quote: ... The sample was applied onto freshly glow-discharged Au 300 mesh R0.6/1 grids (Quantifoil) in a Vitrobot Mark IV at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... The mixture was applied to glow-discharged holey carbon grid (Quantifoil, R2/1, 300 mesh) mounted in a Vitrobot Mark IV (ThermoFisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... The sample-covered carbon was then adsorbed to an EM grid (Quantifoil R3.5/1, Jena Bioscience) and blotted for 9 s using a Vitrobot Mark IV (ThermoFisher ...
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Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogensbioRxiv - Biochemistry 2021Quote: ... the mixture was applied onto a freshly glow-discharged copper R2/1 300 mesh grid (Quantifoil), blotted for 3 s on both sides with blotting force −5 and plunge-frozen in liquid ethane using the Vitrobot Mark IV system (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... 3.5 μL of the culture were applied onto glow-discharged copper EM grids (R2/1, Quantifoil), automatically blotted and plunged into liquid ethane/propane (Tivol et al ...
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bioRxiv - Microbiology 2021Quote: ... and 5 μl of the washed cells were introduced to Au grids (R2/1, 200 mesh; Quantifoil). The grids were fixed in 4% PFA (in TBS ...
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bioRxiv - Biochemistry 2022Quote: ... Solutions were adjusted to OD600≈10 and applied to carbon-coated holey copper grids (Quantifoil R2/1). Grids were glow-discharged for 30 s in oxygen/argon atmosphere of a plasma cleaner (Gatan Solarus 950 ...
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bioRxiv - Microbiology 2021Quote: ... 200-mesh copper grid with holey carbon film (R2/1 Cu 200, Quantifoil Micro Tools GmbH, Germany). The sample was blotted with filter paper and immediately plunge frozen in liquid ethane ...
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bioRxiv - Microbiology 2020Quote: ... CryoET samples were prepared using copper grids with holey carbon support film (200 mesh, R2/1, Quantifoil). The grids were glow-discharged for 30 seconds before we deposited 5 μL cell solution on them ...
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bioRxiv - Neuroscience 2021Quote: Sample volumes of 3.5 µl were applied to freshly glow-discharged R3.5/1 holey carbon grids (Quantifoil) and vitrified using a Mark IV Vitrobot (Thermo Fischer Scientific ...
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bioRxiv - Biochemistry 2022Quote: TEM gold grids with Holey Carbon support film (Quantifoil Micro Tools GmbH, Au 200 mesh R3.5/1) were glow-discharged in a plasma cleaner (Diener electronic GmbH ...
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bioRxiv - Biophysics 2023Quote: ... 10 µL of samples were placed on top of a glow-discharged Au200 R2/1 grids (Quantifoil) and incubated in a 100%-humidity controlled environment ...
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bioRxiv - Molecular Biology 2020Quote: ... applied to plasma-cleaned 200-mesh Cu R2/1 or 300-mesh Au R1.2/1.3 grids (both Quantifoil) and plunge-frozen in liquid ethane ...
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bioRxiv - Biophysics 2020Quote: ... with 2.3 M sucrose9 and transferred to holy carbon finder grids (R3.5/1 Copper NH2 - Quantifoil, Jena, Germany). Sections were stored at 4 °C until further processing.
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bioRxiv - Biochemistry 2021Quote: ... 1 mM TCEP and adsorbed to glow-discharged holey carbon-coated grids (Quantifoil 300 mesh, Au R1.2/1.3) for 10 s ...
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bioRxiv - Microbiology 2020Quote: ... Five microliters of cell suspension were deposited on holey carbon electron microscopy grids (200 mesh, R2/1, Quantifoil), which had been freshly glow-discharged for ∼30 seconds ...
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bioRxiv - Immunology 2021Quote: ... The resulting EV suspension was spotted onto a glow discharged holey carbon mesh copper grids (Quantifoil, R2/1) and incubated at room temperature for 4 mins 45 seconds before 5μL of 0.22μm filtered 2% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.5 µL of fibril solution was applied to freshly glow-discharged R2/1 holey carbon film grids (Quantifoil). After the grids were blotted for 12 seconds at a blot force of 10 ...
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bioRxiv - Biophysics 2023Quote: Three microliters of the purified CLC-2 or CLC-2/AK-42 mixture was applied to glow-discharged copper Quantifoil R1.2/1.3 or R2/1 holey carbon grids (Quantifoil). Grids were incubated for 15 s ...
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bioRxiv - Biochemistry 2023Quote: ... 3.5 μL of HTNV-L at 1 µM was deposited on glow-discharged (25 mA, 45 s) UltraAuFoil 300 mesh R1.2/1.3 grids (Quantifoil). The grid was blotted for 3s (blot force 1 ...
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bioRxiv - Microbiology 2022Quote: Cryo-ET samples were prepared using copper grids with holey carbon support film (200 mesh, R2/1, Quantifoil). The grids were glow-discharged for ∼30 s before depositing 5 µl of cell solution onto them ...
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bioRxiv - Biophysics 2023Quote: ... Aliquots of 4 μl of protein samples (0.3 mg ml-1 or ∼6 μM) were applied to glow-discharged R1.2/1.3 200 Cu mesh grids (Quantifoil). After 30 s ...
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bioRxiv - Biochemistry 2024Quote: ... The carbon was then fished with a glow-discharged holey carbon grid (Quantifoil R3.5/1, copper, mesh 200). 4 µL of dialysis buffer was added to the grid ...
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bioRxiv - Biophysics 2024Quote: ... 200 mesh gold Quantifoil R2/1 grids with a SiO2 support layer (Quantifoil, Cat No N1-S15nAu20-01) were glow discharged and treated with PLL-g-PEG (SuSoS ...
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bioRxiv - Microbiology 2020Quote: ... mobilis Zm4 or Zm6 cells were applied to glow-discharged holey carbon grids (Quantifoil R3.5/1 Cu 200-mesh) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... A volume of 4 μL cell suspension at a concentration of 3.07-3.5×107 cells/ml was directly applied to glow-discharged holey carbon R2/1 Cu 200 mesh grids (Quantifoil). After 5 s back-side blotting ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μL of sample were pipetted onto a freshly glow discharged (PELCO easiGlow, 25 mA, 45 sec) 300 mesh Cu 0.6/1 grid (Quantifoil) and blotted for 4 s at strength 4 ...
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bioRxiv - Microbiology 2024Quote: ... the sample was applied to a holey carbon-coated copper grid for electron microscopy (R2/1, mesh 200; Quantifoil), blotted and vitrified by plunging into liquid ethane using a Vitrobot Mark IV (FEI) ...
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bioRxiv - Immunology 2024Quote: ... The complex (1 mg/mL) was then applied onto the glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3) using an FEI Vitrobot IV ...
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bioRxiv - Neuroscience 2024Quote: ... Double application of 3 μL protein (∼0.6 mg/mL) SYM2081 complex was carried out on glow-discharged gold grids (R 0.6/1, 300 mesh, Quantifoil). The grids were blotted for 4.5 s and 3.5 s ...
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bioRxiv - Microbiology 2024Quote: ... after applying 2.5 µl of sample to a cryoEM grid (Quantifoil Cu/Rh R3.5/1, UltrAuFoil R 1.2/1.3) as described previously56.
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bioRxiv - Biophysics 2024Quote: ... 3.5 µL sample was applied to a freshly glow-discharged (PELCO easiGlow, 30 mA for 120s) 0.6/1 UltrAuFoil grid (Quantifoil) (Russo and Passmore ...
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bioRxiv - Biochemistry 2022Quote: ... An aliquot of 3.5 μl of sample was placed on the glow-discharged cryo-EM grid (Quantifoil R1.2/1.3 or Quantifoil R2/1), blotted and plunge-frozen in liquid ethane using a Mark VI Vitrobot instrument maintained at 100% humidity with blot force 20 and blot time of 3 seconds ...
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bioRxiv - Cell Biology 2021Quote: MEFs expressing emerald-Vimentin are cultured on glow-discharged holey carbon coated EM grids (Au R2/1, 200 mesh, Quantifoil) for 16 h at 37°C in a humidified CO2 incubator ...
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bioRxiv - Biochemistry 2022Quote: 3 µl of prepared complexes at a concentration of approximately 1 mg/ml was applied to a glow-discharged Quantifoil 300 mesh copper R1.2/1.3 grids (Quantifoil Micro Tools). The grids were then flash frozen in liquid ethane using a Thermo Fisher Scientific Vitrobot IV (0-0.5 s waiting time ...
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bioRxiv - Biochemistry 2021Quote: ... Samples of ΦKZ nvRNAP were adsorbed to a thin film of graphene oxide deposited upon the surface of holey carbon copper grids (R2/1, 300 mesh, Quantifoil). Grids were blotted for 1-2 seconds before plunge freezing in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: An aliquot of 2.5 microliters of purified drCALHM1 sample was applied onto a glow discharged holey carbon film grid (200 mesh, R2/1, Quantifoil). The grid was blotted and flash-frozen in liquid ethane with FEI Vitrobot Mark IV ...
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bioRxiv - Cell Biology 2020Quote: ... Isolated centrioles were then deposited onto 200-mesh copper EM grids coated with holey carbon (R3.5/1, Quantifoil Micro Tools) and plunge-frozen in a liquid ethane/propane mixture ...
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bioRxiv - Cell Biology 2021Quote: K5/K14_1 cells were seeded on glow-discharged Collagen I coated holey carbon gold EM grids (Au R2/1, 200 mesh, Quantifoil) and incubated overnight at 32 °C and in 5% CO2 ...
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bioRxiv - Cell Biology 2020Quote: Samples of concentrated TSCCFL were adsorbed to a thin film of graphene oxide deposited upon the surface of holey carbon copper grids (R2/1, 300 mesh, Quantifoil). Grids were blotted for 2-3 seconds before plunge freezing in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: 3 µl of prepared complexes at a concentration of approximately 1 mg/mL was applied to UltrAuFoil 300 mesh gold R1.2/1.3 grids (Quantifoil Micro Tools), freshly glow discharged with an Edwards S150B glow discharger for 1 min 15 sec ...
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bioRxiv - Biochemistry 2022Quote: ... Three μL of sample at 5 μM was adsorbed onto glow-discharged copper Quantifoil R2/1 holey carbon grids (Quantifoil). Grids were glow discharged 45 seconds at 25 mA using an EMITECH K950 apparatus ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μl of protein at a concentration of approximately 10 mg ml−1 were loaded onto glow-discharged holey carbon grids (Quantifoil Au 300 mesh R1.2/1.3 or Quantifoil Cu/Rh 300 mesh R1.2/1.3) ...
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bioRxiv - Biochemistry 2023Quote: ... 3 µl of the VIF-ΔT solution was applied onto glow-discharged holey carbon EM grids (Cu R2/1, 200 mesh, Quantifoil), which were subsequently blotted manually and plunge frozen in liquid ethane.
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bioRxiv - Biochemistry 2023Quote: ... 2.8 µl of F-actin sample was applied to a glow-discharged R2/1 Cu 300 mesh holey-carbon grid (Quantifoil). Excess solution was blotted away and the grids were plunge frozen in liquid ethane or a liquid ethane/propane mixture using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... 4 µL of chloroplasts solution was placed onto R2/1 or R1.2/1.3 carbon-coated 200-mesh copper EM grids (Quantifoil Micro Tools), blotted for 7-8 seconds from the back side (front side pad covered with a Teflon sheet ...
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bioRxiv - Biophysics 2024Quote: ... 3 µL of purified AE1 at 5 mg/mL was added to a glow discharged (PELCO easiGlow) 0.6/1 µm holey gold grid (Quantifoil UltrAuFoil) and blotted for 4-6 s at 4°C and 100% humidity using the Vitrobot Mark IV system (Thermofisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... gold EM grids with Quantifoil holey carbon film (Quantifoil, R2/1, 300 mesh, gold; Electron Microscopy Sciences, Q3100AR1; or Quantifoil, R3.5/1 ...
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bioRxiv - Bioengineering 2022Quote: ... A total of 3.5 μL of TP–AG treated MT samples was applied to a glow discharged holey carbon grid (Quantifoil R2/1). The grids were blotted ...