Labshake search
Citations for Quantifoil Micro Tools :
551 - 600 of 610 citations for 2 4 Methoxyethoxy 3 methyl 2 pyridinyl methylthio benzimidazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... A total of 4 µL of cell suspension (1500–2000 cells/µL) were applied to holey carbon R2/1 or R1/4 copper TEM grids (Quantifoil), and cells were plunge frozen on a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... 3 μL of the purified MC1R-Gs complex was applied to a glow-discharged holey carbon EM grid (Quantifoil, Au 300 R1.2/1.3) in a Vitrobot chamber (FEI Vitrobot Mark IV) ...
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bioRxiv - Biochemistry 2020Quote: ... aliquots (3 μL) of the purified protein concentrated to ~ 10 mg/mL were placed on glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3). For KCC3-DIOA samples ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of the sample was applied to a glow-discharged R2/1 300 mesh holey carbon copper grid (Quantifoil Micro Tools GmbH) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of diluted sample was applied to a glow-discharged (R2/1 300 mesh holey carbon copper grid (Quantifoil Micro Tools GmbH) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Biochemistry 2022Quote: ... Peak fractions were collected and 3 μl of TSEN/pre-tRNA complex were applied onto freshly glow-discharged copper grids (Quantifoil, Cu R1.2/1.3) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: The purified complex samples (3 μL at 5-8 mg/mL) were applied to glow-discharged holey grids (Quantifoil R1.2/1.3, 300 mesh) and subsequently vitrified using a Vitrobot Mark IV (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μL of sample (10 μM EGFP in PBS) was deposited onto glow-discharged grids (Electron Microscopy Science and Quantifoil – see Table 1), prior to blotting the grids from the sample-side for 3 seconds.
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bioRxiv - Molecular Biology 2023Quote: ... A 2.5-3 μL aliquot of protein sample was dropped onto the glow discharged holey carbon grids (Quantifoil Au R1.2/1.3, 300 mesh), blotted with a Vitrobot Mark IV (ThemoFisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: Three µl of purified PSI at a concentration of 3 mg/ml was applied to the glow-discharged holey carbon grids (Cu QUANTIFOIL® R1.2/1.3), then vitrified using a Leica GP2 plunge freezer (2.5 s blot ...
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bioRxiv - Microbiology 2021Quote: ... Aliquots (4 μL) of the protein complex were placed on glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3, 300 mesh). The grids were blotted and flash-frozen in liquid ethane cooled by liquid nitrogen with a Vitrobot apparatus (Mark IV ...
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bioRxiv - Microbiology 2020Quote: Three μl of purified HKU1 HE (4 mg/ml) was dispensed on Quantifoil R1.2/1.3 200-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly glow discharged for 30 seconds at 20 mA using GloQube Glow Discharge system (Quorum Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... cells in suspension (4 μl of ~1,000 cells per μl) were blotted onto R2/1 carbon-coated 200-mesh copper grids (Quantifoil Micro Tools) and plunge frozen in a liquid ethane/propane mixture ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 μL of protein samples were immediately applied to discharged 300-mesh Au R1.2/1.3 grids (Quantifoil, Micro Tools GmbH, Germany). Grids were blotted for 4 s and plunged into liquid ethane using an FEI Mark IV Vitrobot operated at 8°C and 100% humidity ...
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bioRxiv - Cell Biology 2023Quote: ... 4 μl of the sample was applied to glow discharged holey carbon-coated 200 mesh copper EM grids (Quantifoil Micro Tools), vitrified as above at 4°C and 100 % humidity.
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bioRxiv - Immunology 2024Quote: Neutrophils were stimulated with 100 nM LTB4 in mHBSS for 15 mins at 37 °C in 35 mm glass bottom dishes containing glow-discharged Au SiO2 R1/4 200 mesh grids (Quantifoil GmbH) precoated with 25 μg/ml fibrinogen in DPBS ...
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bioRxiv - Immunology 2024Quote: ... a 4 μL aliquot of protein complex was applied to glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3, 300-mesh) or graphene oxide (GO)-coated holey-carbon gold grids (EMR ...
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bioRxiv - Biophysics 2021Quote: ... A suspension containing a mixture of 10 nm protein A coated colloidal gold (Aurion) and Ebola VLPs was applied with a volume of 3 µl on a glow discharged EM grid (Quantifoil, 200 Mesh, R2/1). The grid was blotted using No.1 Whatman paper for 3 sec from the back-side in a chamber with controlled humidity set to 70% and the temperature set to 24°C.
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bioRxiv - Biochemistry 2020Quote: An aliquot of 3 μL of SAM complex sample was applied to freshly glow-discharged holey carbon grid (Quantifoil R1.2/1.3, copper, 300 mesh). The grids were blotted for 6 s and plunge-frozen in liquid nitrogen-cooled liquid ethane using an FEI Vitrobot Mark IV plunger ...
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bioRxiv - Neuroscience 2022Quote: Extracted filaments were centrifuged at 3,000 g for 3 min and applied to glow-discharged holey carbon gold grids (Quantifoil Au R1.2/1.3, 300 mesh), which were glow-discharged with an Edwards (S150B ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were centrifuged at 3,000 g for 3 min and applied to glow-discharged holey carbon gold grids (Quantifoil Au R1.2/1.3, 300 mesh), which were glow-discharged with an Edwards (S150B ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Grids were prepared by applying 3 μL of purified CB1-Gi complex at 12 mg/ml to glow-discharged holey carbon gold grids (Quantifoil R1.2/1.3, 200 mesh). The grids were blotted using a Vitrobot Mark IV (FEI ...
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bioRxiv - Neuroscience 2022Quote: Extracted filaments were centrifuged at 3,000g for 3 min and applied to glow-discharged holey carbon gold grids (Quantifoil Au R1.2/1.3, 300 mesh), which were glow-discharged with an Edwards (S150B ...
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bioRxiv - Biochemistry 2023Quote: ... The detergent was added shortly before applying 3 μL of UBR5Dimer∼UbD∼UBE2D2 to freshly glow-discharged holey carbon grids (Quantifoil, R1.2/1.3, 200 mesh). The sample was consequently plunge-frozen into liquid ethane at 95 % humidity and 4°C (blot force 3 ...
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bioRxiv - Molecular Biology 2023Quote: Freshly purified ZorAB sample was concentrated to 2-3 mg/mL and 2.7 μL protein was applied onto the glow-discharged (30 s, 5 mA) grids (Quantifoil R0.6/1 300 mesh Au) and plunge-frozen into liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Molecular Biology 2021Quote: ... bremelanotide– or THIQ–bound MC4R–Gs complexes at ∼ 4 mg/mL were applied onto glow-discharged holey carbon grids (Quantifoil R1.2/1.3). Excess samples were blotted away and the grids were vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFischer Scientific).
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bioRxiv - Genomics 2020Quote: ... Vitrified specimens were prepared by adding 4 μl disome samples at the concentration of ~ 150 nM to a glow-discharged holey carbon grid (Quantifoil R1.2/1.3), which was covered with a freshly made thin carbon film ...
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bioRxiv - Cell Biology 2020Quote: ... The MEFs were applied onto glow-discharged EM grids with a silicon-oxide support film (R1/4, Au mesh; Quantifoil, Jena, Germany). After incubation ...
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bioRxiv - Cell Biology 2022Quote: The cryo-EM grid was prepared by applying 3 μl of the protein samples (4 – 6 mg/ml) to glow-discharged Quantifoil R1.2/1.3 300-mesh gold holey carbon grids (Quantifoil, Micro Tools GmbH, Germany). Grids were blotted for 4.0 seconds under 100% humidity at 4°C before being plunged into the liquid ethane using a Mark IV Vitrobot (FEI) ...
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bioRxiv - Cell Biology 2024Quote: ... the sample was gently resuspended and 4 µl was transferred to a glow-discharged holey carbon R2/1 copper grid (Quantifoil Micro Tools) suspended in the chamber of a Vitrobot (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Four µL of the cells were subsequently deposited onto Quantifoil R2/4 200 mesh copper grids (Quantifoil Micro Tools GmbH, Jena, Germany), which were then manually blotted from the back with Whatman® Grade 1 filter paper for approximately 3 – 5 seconds ...
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bioRxiv - Biophysics 2023Quote: ... 4 μl suspended cells were loaded onto the front side of glow-discharged holy-carbon copper grids (Quantifoil R1.2/1.3, 200 mesh), and 2 μl medium was subsequently added to the back side of the grids ...
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bioRxiv - Biochemistry 2022Quote: ... Several serial dilutions were prepared and 3 µl of each sample was loaded on Quantifoil R2/2Cu/Rh 300 holey-carbon-supported grids (Quantifoil Micro Tools GmbH, Jena, Germany). Initial cryo-EM sample preparations showed clear protein aggregation ...
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bioRxiv - Biophysics 2022Quote: Cryo-EM grids were prepared by applying 3 μL of sample (at the native 1 nM concentration) on glow discharged holey gold grids (Quantifoil Cu 1.2/1.3 400 mesh). Excess sample was removed by blotting with filter paper for 4 sec prior to plunge-freezing in liquid ethane using a FEI Vitrobot Mark IV at 100% humidity and 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: Cryo-EM grids were prepared by applying 3 µL sample (at a protein concentration of 1 mg/ml) on glow-discharged holey gold grids (Quantifoil Au 1.2/1.3 300 mesh). Excess sample was removed by blotting with filter paper for 2-3 s before plunge-freezing in liquid ethane (cooled to −181 °C ...
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bioRxiv - Immunology 2020Quote: An aliquot of 3 μl purified protein of rNLRP1-rDPP9 or rNLRP1FIIND S969A-rDPP9 complex was applied to holey carbon grids (Quantifoil Au 1.2/1.3, 300 mesh), which were glow-discharged for 30 s at middle level in Harrick Plasma after 2 min evacuation ...
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bioRxiv - Biophysics 2023Quote: 2.5μL aliquots of Fks1 at a concentration of about 3 mg/mL were placed on glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3, 300 mesh) and were flash-frozen in liquid ethane using a FEI Vitrobot Mark IV ...
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bioRxiv - Molecular Biology 2023Quote: 3 µL of the human CDCA7264-371 in complex with the nucleosome harboring a single hemimethylated CpG in the 3’-linker DNA was applied onto the glow-discharged holey carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh). The grids were plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: Vitrified grids were prepared by applying 3 µl of 1 uM complex sample to a freshly glow- discharged holey carbon grids (Quantifoil R1.2/1.3 Au 200 mesh). The sample was blotted for 3-5 s at 4 ℃ with 100% humidity and plunged-frozen in liquid nitrogen cooled ethane (FEI TM ...
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bioRxiv - Biochemistry 2021Quote: 3.5 µl protein solution containing 0.05 µM CUL4-NEDD8/ROC1/DDB1/DCAF1-CtD/Vprmus/SAMHD1 complex and 0.25 µM UBCH5C-ubiquitin conjugate (S4 A, B Fig) were applied to a 300 mesh Quantifoil R2/4 Cu/Rh holey carbon grid (Quantifoil Micro Tools GmbH) coated with an additional thin carbon film as sample support and stained with 2% uranyl acetate for initial characterisation ...
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bioRxiv - Biochemistry 2022Quote: ... Spike trimer - Fab complexes (4 μl) were applied to the pre-glow-discharged holey carbon grids (Quantifoil grid, Cu 300 mesh, R1.2/1.3). The grids were then blotted for 2 seconds with filter paper in 100% relative humidity and 8 °C and plunged into the liquid ethane to freeze samples using FEI Vitrobot system (FEI).
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bioRxiv - Molecular Biology 2022Quote: ... An aliquot of 4 μL protein sample of Spike protein was applied onto a glow-discharged 300 mesh grid (Quantifoil Au R1.2/1.3) and GraFuture™-RGO grid which was supported with a thin layer of RGO (reduced graphene oxide) ...
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bioRxiv - Molecular Biology 2023Quote: ... a 4 µl sample of purified complex was applied to glow-discharged R2/1 300 mesh holey carbon copper grids (Quantifoil Micro Tools GmbH), using a conc ...
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bioRxiv - Cell Biology 2023Quote: 4 ul of Nannochloropsis suspensions (4 x 107 cells mL−1) were pipetted onto a 300 mesh gold Quantifoil grid with 2um holes (Quantifoil Micro Tools, GmbH) and manually blotted from the opposite side in a Vitrobot Mark IV (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: 4 μL of Fcα–J–FcRL4 (0.3 mg/mL) were applied to glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3, 300 mesh) or Ni-Ti grids (CryoMatrix ...
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bioRxiv - Immunology 2023Quote: ... respectively) and 3 μl were kept 5 min at RT until their application onto glow-discharged holey carbon grids (Quantifoil, Au 300 mesh, R 0.6/1). The grids were blotted and then plunged into liquid ethane using a FEI Vitrobot Mark IV at 20°C and 95% relative humidity ...
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bioRxiv - Biophysics 2024Quote: 3 μL of concentrated protein sample was applied to a glow-discharged (30 s at 15 mA) grid (Quantifoil Au or Cu R1.2/1.3 300 mesh) and after a wait time of 5 s ...
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bioRxiv - Biochemistry 2024Quote: 3µl of the AtGDH-AKG bound binary complex (3 mg/ml) was applied to freshly glow-discharged holey carbon grids (Quantifoil Au R 0.6/1.0 300 mesh). For the apo form AnGDH dataset ...
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bioRxiv - Biophysics 2020Quote: ... 2.5 μl samples of GDN-purified proteins at a concentration of 1.5-3 mg ml−1 were applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3 or R0.6/1 Au 200 mesh). Excess liquid was removed in a controlled environment (4°C and 100% relative humidity ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 μl of protein sample (about 1.5 mg/ml) was applied onto a glow-discharged holey carbon grid (Quantifoil Au, R1.2/1.3, 300 mesh). At a temperature of 4°C and under a humidity of 100% ...