Labshake search
Citations for Quantifoil Micro Tools :
551 - 600 of 610 citations for 2' Chloro 3 2 3 dimethylphenyl 4' fluoropropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Primary cultured neurons were seeded onto holey gold EM grids (Quantifoil R 1/4 gold 200 mesh). The fiducial marker solution was prepared as for neocortical synaptosomes ...
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bioRxiv - Cell Biology 2024Quote: ... Quantifoil Holey SiO2 R 1/4 Au 200 mesh EM grids (Quantifoil Micro Tools #N1-s13nAi20-01) were glow discharged on both sides in a Pelco easyglow for 45 s at 0.37 mBar and 15 mA ...
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bioRxiv - Biophysics 2023Quote: ... 4 µL fresh sample was applied on glow-discharged Cu holey carbon grids (Quantifoil R 1.2/1.3) and incubated for 1 min at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... 4 μL of the TnsBCTD-TnsC-TniQ sample was loaded on UltrAuFoil R1.2/1.3 gold grids (Quantifoil) and vitrified using the Mark IV Vitrobot (ThermoFisher ...
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bioRxiv - Biochemistry 2021Quote: ... 3 microlitres of undiluted eluate and of eluate which was diluted 1:2 or 1:4 in buffer A200 were blotted onto holey carbon R 1.2/1.3 copper 300 mesh grids (Quantifoil) and vitrified in liquid ethane using a Vitrobot MarkIV device (Thermofisher) ...
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bioRxiv - Biophysics 2021Quote: ... 4 µL of aldolase sample were applied on UltrAuFoil R1.2/1.3 300-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly plasma cleaned for 6 seconds at 15 Watts (75% nitrogen/25% oxygen atmosphere ...
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bioRxiv - Biophysics 2021Quote: ... 4 µL of apoferritin sample were applied on UltrAuFoil R1.2/1.3 300-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly plasma cleaned for 7 seconds at 15 Watts (75% nitrogen/25% oxygen atmosphere ...
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bioRxiv - Biophysics 2021Quote: The LlCsm RNP and RNP-target RNA samples at approximately 0.5 mg/ml were separately applied in 4 μL volume onto glow-discharged UltrAuFoil 300 mesh R1.2/1.3 grids (Quantifoil), blotted for 3 s at 88% humidity and flash-frozen in liquid ethane using FEI Vitrobot Mark IV ...
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bioRxiv - Biochemistry 2024Quote: ... 4 uL of the concentrated sample was applied to freshly glow-discharged R2/1 holey carbon grids (Quantifoil), blotted with blot force of 5 for 5 s using a Vitrobot Mark IV (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... for 15 min before 4 μL of sample was applied to a graphene oxide coated 1.2/1.3 UltrAuFoil grids (Quantifoil) at 4°C under 100% humidity86 ...
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bioRxiv - Microbiology 2022Quote: ... Aliquots of 4 μL samples were applied to glow-discharged holey carbon girds (Cu, R1.2/1.3, 300 mesh, Quantifoil). The grids were blotted with force 2 for 3 seconds and plunged into liquid ethane using a Vitrobot (FEI Thermo Fisher) ...
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bioRxiv - Cell Biology 2024Quote: ... 3.5 µl cell suspension applied on glow-discharged (Pelco easiGlow) R1/4 SiO2 gold 200 mesh grids (Quantifoil), blotted for 2 sec from the backside and was plunge-frozen on a Leica EM GP2 plunger at 20 °C ...
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bioRxiv - Biochemistry 2020Quote: ... KBP was diluted to 0.3 mg/ml in KBP dilution buffer and 4 μl were applied to glow-discharged 1.2/1.3 AuFoil gold grids (Quantifoil®). For the third approach ...
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bioRxiv - Molecular Biology 2021Quote: Purified mtLSU or 55S mitoribosome samples (4 μL) were applied to freshly glow discharged R 3.5/1 holey carbon grids (Quantifoil) that were precoated with a 2-3 nm carbon layer using a Leica EM ACE600 coater ...
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bioRxiv - Microbiology 2022Quote: ... A 4□μl volume of SsoCsm complex was applied to holey carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh) covered by a graphene oxide layer (27) ...
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bioRxiv - Biophysics 2024Quote: ... For plunge freezing the cells were pipetted (4 μl) on EM grids (gold/200mesh/R2.2 either UltrAufoil or Quantifoil) in a humidified chamber (98% RH) ...
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bioRxiv - Cell Biology 2024Quote: ... 3.5 µl of 4 mg/mL proteasomal complexes was applied to glow-discharged R0.6/1 UltrAuFoil 300 mesh grids (Quantifoil) and frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... For plunge freezing the cells were pipetted (4 µl) on EM grids (gold/200mesh/R2.2 either UltrAufoil or Quantifoil) in a humidified chamber (98% RH) ...
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bioRxiv - Biophysics 2021Quote: ... Log phase cultures were centrifuged at 2000 g for 2 minutes to concentrate the cells 10 times and 4 μl was directly applied to a glow-discharged holey carbon R2/1 Cu 200 mesh grid (Quantifoil). Plunge-freezing was performed using a VitroBot Mark 4 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... trehalose solution to mitigate crystalline ice formation and 4 μL of this mixture immediately applied to a R2/1 Cu 200 grid (Quantifoil), which had been glow-discharged for 1 min at 0.19 mbar and 20 mA in a PELCO easiGlow device shortly before use ...
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bioRxiv - Cell Biology 2024Quote: ... 4 μl of the cell suspension was applied onto a glow-discharged holey SiO2 grid (R1/4, Au, 200 mesh, Quantifoil) mounted on in the plunger chamber ...
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bioRxiv - Biophysics 2023Quote: ... 4 µL fresh sample (0.48 mg/mL) was applied on glow-discharged Au holey carbon grids (Quantifoil R 0.6/1) and the following operation were the same as describe above for preparing the sample of gMCM8/9 complex.
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bioRxiv - Biochemistry 2023Quote: ... 4 μL of Fcμ–J–CD5L was applied to glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3, 300 mesh), blotted with filter paper in 100% humidity at 4 °C ...
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bioRxiv - Biophysics 2022Quote: 3.5 μl of protein samples at 4-6 mg/ml were applied to glow-discharged QF R1.2/1.3 300 mesh gold grids (Quantifoil, Großlöbichau, Germany). Grids were blotted for 3 s at 4°C and 100% humidity and plunge-frozen into liquid ethane using FEI Mark IV Vitrobot (FEI ...
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bioRxiv - Plant Biology 2022Quote: ... then 4 µl of the sample was applied to glow-discharged holey carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh). The grid was blotted for 4s and then plunged into liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Molecular Biology 2023Quote: 1.8 × 105 A549 or A549-IFITM3 cells were seeded on glow-discharged 200 mesh gold grids with a holey SiO2 film with R1/4 spacing (Quantifoil) placed in a 35 mm dish and cultured in 2 ml complete growth medium (DMEM-F12 ...
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bioRxiv - Cell Biology 2024Quote: Cells were seeded and grown overnight on Quantifoil carbon film gold grids (Quantifoil R 1/4 on 200 gold mesh) coated with 10 µg/ml FN ...
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bioRxiv - Biophysics 2024Quote: ... Microtubules (4 µl drop of 40 µM dilution) were placed onto a glow-discharged holey carbon grid (R2/1; Quantifoil) prepared on a JEC-3000FC (JEOL) ...
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bioRxiv - Cell Biology 2024Quote: ... A total of 4 µL of cell suspension (1500–2000 cells/µL) were applied to holey carbon R2/1 or R1/4 copper TEM grids (Quantifoil), and cells were plunge frozen on a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Aliquots (4 μL) of the protein complex were placed on glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3, 300 mesh). The grids were blotted and flash-frozen in liquid ethane cooled by liquid nitrogen with a Vitrobot apparatus (Mark IV ...
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bioRxiv - Microbiology 2020Quote: Three μl of purified HKU1 HE (4 mg/ml) was dispensed on Quantifoil R1.2/1.3 200-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly glow discharged for 30 seconds at 20 mA using GloQube Glow Discharge system (Quorum Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... cells in suspension (4 μl of ~1,000 cells per μl) were blotted onto R2/1 carbon-coated 200-mesh copper grids (Quantifoil Micro Tools) and plunge frozen in a liquid ethane/propane mixture ...
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bioRxiv - Cell Biology 2023Quote: ... 4 μl of the sample was applied to glow discharged holey carbon-coated 200 mesh copper EM grids (Quantifoil Micro Tools), vitrified as above at 4°C and 100 % humidity.
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bioRxiv - Molecular Biology 2022Quote: ... 4 μL of protein samples were immediately applied to discharged 300-mesh Au R1.2/1.3 grids (Quantifoil, Micro Tools GmbH, Germany). Grids were blotted for 4 s and plunged into liquid ethane using an FEI Mark IV Vitrobot operated at 8°C and 100% humidity ...
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bioRxiv - Immunology 2024Quote: Neutrophils were stimulated with 100 nM LTB4 in mHBSS for 15 mins at 37 °C in 35 mm glass bottom dishes containing glow-discharged Au SiO2 R1/4 200 mesh grids (Quantifoil GmbH) precoated with 25 μg/ml fibrinogen in DPBS ...
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bioRxiv - Immunology 2024Quote: ... a 4 μL aliquot of protein complex was applied to glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3, 300-mesh) or graphene oxide (GO)-coated holey-carbon gold grids (EMR ...
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bioRxiv - Molecular Biology 2021Quote: ... bremelanotide– or THIQ–bound MC4R–Gs complexes at ∼ 4 mg/mL were applied onto glow-discharged holey carbon grids (Quantifoil R1.2/1.3). Excess samples were blotted away and the grids were vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFischer Scientific).
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bioRxiv - Genomics 2020Quote: ... Vitrified specimens were prepared by adding 4 μl disome samples at the concentration of ~ 150 nM to a glow-discharged holey carbon grid (Quantifoil R1.2/1.3), which was covered with a freshly made thin carbon film ...
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bioRxiv - Cell Biology 2020Quote: ... The MEFs were applied onto glow-discharged EM grids with a silicon-oxide support film (R1/4, Au mesh; Quantifoil, Jena, Germany). After incubation ...
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bioRxiv - Cell Biology 2023Quote: ... Four µL of the cells were subsequently deposited onto Quantifoil R2/4 200 mesh copper grids (Quantifoil Micro Tools GmbH, Jena, Germany), which were then manually blotted from the back with Whatman® Grade 1 filter paper for approximately 3 – 5 seconds ...
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bioRxiv - Cell Biology 2024Quote: ... the sample was gently resuspended and 4 µl was transferred to a glow-discharged holey carbon R2/1 copper grid (Quantifoil Micro Tools) suspended in the chamber of a Vitrobot (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... 4 μl suspended cells were loaded onto the front side of glow-discharged holy-carbon copper grids (Quantifoil R1.2/1.3, 200 mesh), and 2 μl medium was subsequently added to the back side of the grids ...
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bioRxiv - Cell Biology 2022Quote: The cryo-EM grid was prepared by applying 3 μl of the protein samples (4 – 6 mg/ml) to glow-discharged Quantifoil R1.2/1.3 300-mesh gold holey carbon grids (Quantifoil, Micro Tools GmbH, Germany). Grids were blotted for 4.0 seconds under 100% humidity at 4°C before being plunged into the liquid ethane using a Mark IV Vitrobot (FEI) ...
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bioRxiv - Biochemistry 2021Quote: 3.5 µl protein solution containing 0.05 µM CUL4-NEDD8/ROC1/DDB1/DCAF1-CtD/Vprmus/SAMHD1 complex and 0.25 µM UBCH5C-ubiquitin conjugate (S4 A, B Fig) were applied to a 300 mesh Quantifoil R2/4 Cu/Rh holey carbon grid (Quantifoil Micro Tools GmbH) coated with an additional thin carbon film as sample support and stained with 2% uranyl acetate for initial characterisation ...
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bioRxiv - Biochemistry 2022Quote: ... Spike trimer - Fab complexes (4 μl) were applied to the pre-glow-discharged holey carbon grids (Quantifoil grid, Cu 300 mesh, R1.2/1.3). The grids were then blotted for 2 seconds with filter paper in 100% relative humidity and 8 °C and plunged into the liquid ethane to freeze samples using FEI Vitrobot system (FEI).
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bioRxiv - Molecular Biology 2023Quote: ... a 4 µl sample of purified complex was applied to glow-discharged R2/1 300 mesh holey carbon copper grids (Quantifoil Micro Tools GmbH), using a conc ...
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bioRxiv - Cell Biology 2023Quote: 4 ul of Nannochloropsis suspensions (4 x 107 cells mL−1) were pipetted onto a 300 mesh gold Quantifoil grid with 2um holes (Quantifoil Micro Tools, GmbH) and manually blotted from the opposite side in a Vitrobot Mark IV (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: 4 μL of Fcα–J–FcRL4 (0.3 mg/mL) were applied to glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3, 300 mesh) or Ni-Ti grids (CryoMatrix ...
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bioRxiv - Molecular Biology 2022Quote: ... An aliquot of 4 μL protein sample of Spike protein was applied onto a glow-discharged 300 mesh grid (Quantifoil Au R1.2/1.3) and GraFuture™-RGO grid which was supported with a thin layer of RGO (reduced graphene oxide) ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 μl of protein sample (about 1.5 mg/ml) was applied onto a glow-discharged holey carbon grid (Quantifoil Au, R1.2/1.3, 300 mesh). At a temperature of 4°C and under a humidity of 100% ...