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Citations for Quantifoil Micro Tools :
451 - 500 of 509 citations for 4R 5S 2 5 Fluoropyridin 2 yl 4 5 diphenyl 4 5 dihydrooxazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 4 µl of the solution was applied to a glow-discharged (30s at 35 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools). After removing excess liquid by blotting the grid from the back side with Whatman filter paper for 2 s ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were applied to freshly glow-discharged (Edwards S150B, 45 sec, 30-50 mA, 1,2kV, 10-2 mbar) copper holey carbon grids (Quantifoil, Cu-R1.2/1.3) under 100% humidity ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μl of the mixture was deposited on glow discharged R2/2 Copper 300 mesh holey carbon-coated support grids (Quantifoil, Jena, Germany) along with 1 μl of HEPES pH 10 and 1 μl of hydrogen peroxide 0.9 % ...
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bioRxiv - Neuroscience 2021Quote: ... 400 mesh Quantifoil R1.2/1.3 grids with empty holes and 400 mesh Quantifoil R1.2/1.3 grids coated with a 2 nm thin carbon layer (Quantifoil Micro Tools GmbH, Germany). The grids were rendered hydrophilic by glow discharge using a Pelco easiGlow glow discharger (Ted Pella Inc. ...
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bioRxiv - Microbiology 2020Quote: ... Cells were concentrated by centrifugation and 3 μL aliquots of the cell suspension are applied to glow-discharged R2/2 200 mesh copper Quanti-foil grids (Quantifoil Micro Tools), the sample was pre-blotted for 30 seconds ...
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bioRxiv - Cell Biology 2020Quote: ... A 5 µl drop of the chemically fixed cell culture was deposited on a glow-discharged Quantifoil 200 mesh R2/2 electron microscopy grid (Quantifoil, Großlöbichau, Germany) pre-coated with a gold bead solution ...
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bioRxiv - Cell Biology 2020Quote: ... 3.5 µL of gel filtration column eluate (∼0.5 mg/ml) was applied to a holey carbon support (Quantifoil 2/1-4Cu-50) at ∼90% relative humidity ...
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bioRxiv - Biochemistry 2020Quote: ... 3 µL aliquots of 0.1 mg/ml cross-linked ternary complexes were applied to freshly glow-discharged R2/2 Quantifoil 200 mesh copper grids (Quantifoil Micro Tools). Grids were blotted in a climate chamber set to 20 °C and at 95% humidity before plunge frozen in liquid ethane set at −183 °C using a Leica EM GP system (Leica Microsystems) ...
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Computational toolbox for ultrastructural quantitative analysis of filament networks in cryo-ET databioRxiv - Cell Biology 2021Quote: B16-F1 cells were cultured as described above and seeded onto 200 mesh gold holey carbon grids (R2/2-2C; Quantifoil Micro Tools). Prior to cell seeding ...
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bioRxiv - Cell Biology 2021Quote: ... Glow-discharged holey Quantifoil grids with an additional layer of continuous carbon (R2/1 + 2 nm C, Cu 200 mesh grid, Quantifoil Micro Tools) were used ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μl of the resulting mixture was spotted onto glow-discharged copper EM grids (Quantifoil 300 Au mesh, R2/2 carbon film (Quantifoil Micro Tools). The grids were then blotted for 2 or 4 seconds (blot force −10 ...
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bioRxiv - Cell Biology 2022Quote: ... 3 µl of the microtubule sample was deposited at the surface of a glow-discharged holey carbon grid (Quantifoil R2/2, Cu200) in the temperature (35 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were applied to freshly glow-discharged (Edwards S150B, 45 s, 30−50 mA, 1.2 kV, 10−2 mbar) copper holey carbon grids (Quantifoil, Cu-R1.2/1.3) under 100% humidity at 4°C.
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bioRxiv - Biochemistry 2023Quote: 4.5 µL of freshly mixed Nup98 condensate and capsid mixture with protein A-gold (10 nm) were applied onto a glow-discharged Quantifoil R2/2 copper grid (Quantifoil Micro Tools). The grid was blotted in the front for 2.5 s at 15 °C with 90% relative humidity and then plunged into liquid ethane using a Lecia EM GP device (Lecia Microsystem) ...
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bioRxiv - Biophysics 2023Quote: ... Micropatterns covered an area of 8 × 8 squares around the center of the cryo-TEM grids (Quantifoil R1/4 or R1/20, Au 200 mesh, SiO2 film) as previously described31 ...
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bioRxiv - Microbiology 2020Quote: ... 3.5 μl of cell suspension from 80-100 μl of final volume were applied on glow discharged 200-mesh EM copper grids coated with R 2/1 holey SiO2 films (Quantifoil Micro Tool GmbH) and plunge frozen in liquid ethane at ~−184°C using a Leica EM GP grid plunger ...
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bioRxiv - Biophysics 2020Quote: ... 3.5ul of cell suspension were applied on 200-mesh copper grids coated with R 2/1 holey carbon or SiO2 films (Quantifoil Micro Tools GmbH) and plunge frozen in liquid ethane at ~-186°C using a Leica EM GP grid plunger ...
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bioRxiv - Immunology 2022Quote: ... detergent at a final concentration of 0.005 mM was added to each sample and 3 µl of that solution was immediately loaded onto plasma-cleaned QuantiFoil R 2/1 (400-mesh; Quantifoil Micro Tools GmbH) and UltrAuFoil R 1.2/1.3 grids (300-mesh ...
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bioRxiv - Biochemistry 2020Quote: ... 3 μl aliquots of purified mitoribsomes was incubated for 30 s on glow-discharged holey carbon grids (Quantifoil R2/2, 300 mesh, copper) pre-coated with a home-made continuous carbon film with thickness of ~ 27 Å ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μL of urease samples at 1.3 mg/mL with 1 mM BME or with 10 mM SHA were applied to freshly glow-discharged Quantifoil R2/2 holey carbon grids (Quantifoil Micro Tools, Germany). The grids were blotted for 4 seconds ...
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bioRxiv - Immunology 2020Quote: ... and 3 µl was immediately loaded onto plasma cleaned Quantifoil R 2/1 holey carbon copper gri (Cu, 400-mesh, Quantifoil Micro Tools GmbH). Blotted grids were plunge-frozen into nitrogen-cooled liquid ethane.
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bioRxiv - Microbiology 2023Quote: ... Both TBEV strain Neudoerfl and Hypr samples were vitrified in liquid ethane on holey carbon coated copper grids (Quantifoil 2/1, mesh 300, Quantifoil Micro Tools GmbH) using Vitrobot Mark IV (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... 2–3 μL of sample solution was applied to a holey carbon grid (Quantifoil R2/1, Mo 300 mesh; Quantifoil Micro Tools GmbH) covered with a thin amorphous carbon film at 4 °C with 100% humidity ...
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bioRxiv - Cell Biology 2022Quote: ... A 3 µl volume of the Kif5B suspension was first deposited at the surface of a glow-discharged holey carbon grid (Quantifoil R2/2, Cu200) in the temperature (23 °C ...
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bioRxiv - Biophysics 2023Quote: Three microliters of the purified GLV-HP or GLV-CAT sample was placed on a glow-discharged holey carbon grid (Quantifoil R2/2 or R2/1, Cu 300 mesh; Quantifoil Micro Tools GmbH) for 3 s blotting time at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... and an aliquot of 2 μl of this sample was applied onto a plasma cleaned holey carbon grid (Quantifoil, R1.2/1.3, 200 mesh). The grid was blotted with Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: A 5 µl cell suspension containing 10 nm colloidal gold particles were deposited on the copper EM grids with holey carbon supported film (Quantifoil, 200mesh, R1/2). The EM grid were blotted with filter paper (Waterman ...
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bioRxiv - Biochemistry 2023Quote: ... 3µL of sample were frozen in liquid ethane on 2/1 Quantifoil Multi A holey carbon supported grids (Quantifoil, Multi A, 400 mesh) using Vitrobot Mark IV (FEI Company) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Grids were prepared by applying 3.5 μLs of the Z7757 complex or 2 μLs of the Lisuride complex to glow discharged UltrAuFoil holey gold grids (Quantifoil, Au300-R1.2/1.3). The girds containing Z7757 were then vitrified by plunge freezing into a 60/40 mixure of liquid ethane/propane using a Vitrobot mark IV (FEI ...
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bioRxiv - Cell Biology 2024Quote: ... intact MVKCg1 yeasts cells resuspended in PBS were vitrified on Quantifoil Au R2/2 London Finder 300-mesh grids (Quantifoil Micro Tools GmbH) following a similar vitrification procedure as described above for crude membranes ...
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bioRxiv - Biophysics 2024Quote: ... 4 μl sample was applied to each glow-discharged (30 s at 15 mA) QuantiFoil 2/1 Cu 300 grid (Quantifoil Micro Tools GmbH), pre-coated with graphene oxide according to Boland et al (21) ...
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bioRxiv - Biophysics 2024Quote: ... Cryo-EM grids were obtained by flash-freezing 3 µL of purified IPNV particles on glow-discharged holey carbon grids (Quantifoil R2/2, Cu 300 mesh; Quantifoil Micro Tools GmbH). The grids were plunge-frozen in liquid ethane after blotting for 3 s under 4°C ...
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bioRxiv - Cell Biology 2020Quote: 4 µl of the cultured yeast cells (OD600 of 0.4) were added to a glow-discharged (30 seconds at -30 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools GmbH, Jena, Germany), then the grid was rapidly plunge frozen in liquid ethane using a homemade plunge freezer and stored in liquid nitrogen until used ...
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bioRxiv - Biophysics 2022Quote: ... an aliquot of 3 μL of sample was applied onto a glow-discharged holey carbon-coated copper grid (300 mesh, QUANTIFOIL® R 2/1) or holey gold grid (300 mesh ...
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bioRxiv - Biochemistry 2020Quote: ... 2.5 μL aliquots of purified Dnf1p–Lem3p or Dnf2p–Lem3p at a concentration of about 1.5 mg/mL were placed on glow-discharged holey carbon grids (Quantifoil Au R2/2, 300 mesh) and were flash-frozen in liquid ethane using a FEI Vitrobot Mark IV ...
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bioRxiv - Cell Biology 2022Quote: ... 4 µL of the mixture were pipetted onto a copper EM grid with holey carbon film (R2/2, 200 mesh, Quantifoil Micro Tools GmbH, Q43486) that had been freshly glow-discharged for 30 s at -30 mAmp ...
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bioRxiv - Biophysics 2024Quote: A 3 μL of aliquot Msmeg MmpL3 ΔC NDs at a concentration of 0.6 mg/mL was applied to glow-discharged (Quantifoil Cu R2/2, 300 mesh) holey carbon grids for 30 sec and blotted for 2×5 sec at 4°C and 100% humidity ...
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bioRxiv - Biochemistry 2024Quote: ... 3 µl were frozen in liquid ethane with the Vitrobot Mark IV (FEI company) on 2/1 Quantifoil Multi A holey carbon supported grids (Quantifoil, Multi A, 400 mesh). Freezing was carried out with Whatman #1 filter papers at 4°C with a blot force of 0 ...
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bioRxiv - Biochemistry 2021Quote: ... 2.5-μL aliquots of purified Neo1 at a concentration of about 1 mg/mL were placed on glow-discharged holey carbon grids (Quantifoil Au R2/2, 300 mesh) and were flash-frozen in liquid ethane using a FEI Vitrobot Mark IV ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were prepared by depositing 3 μl of the crystalline slurry onto a glow-discharged EM grid (Quantifoil, Cu 200 mesh, R2/2 holey carbon). Excess liquid was blotted away and the sample was vitrified using a Leica GP2 vitrification robot ...
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bioRxiv - Molecular Biology 2022Quote: RNA granule preparations were diluted with RNA Granule buffer to an optical density A260 = 2 U/mL aliquots of the dilution deposited on glow-discharged Quantifoil grids (Quantifoil 1.2/1.3, 300 mesh copper) pre-coated with a continuous layer of 3 nm carbon ...
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bioRxiv - Molecular Biology 2022Quote: 3 μL of ~120 nM mitoribosome was applied onto a glow-discharged (20 mA for 30 sec) holey-carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ~3 nm thickness ...
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bioRxiv - Biophysics 2020Quote: ... 2 μL of a 1 in 6 dilution of the batch crystallisation was applied to the carbon side of glow discharged R2/2 Quantifoil™ grids (Quantifoil Micro Tools, Jena, Germany). Excess liquid was removed by blotting for 4-6 s with a Vitrobot (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... 3.5 μl of that mixture was transferred to a 3 mm freshly glow-discharged Quantifoil R2/2 Cu 300 mesh holey carbon grid (Quantifoil Micro Tools GmbH, Jena, Germany). Excess liquid was blotted away using a Leica EM GP plunge-freezer at room temperature and 80% humidity (blot time 2-3 sec ...
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bioRxiv - Biochemistry 2023Quote: 3 μL of ∼120 nM mitoribosome was applied onto a glow-discharged (20 mA for 30 sec) holey- carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ∼3 nm thickness ...
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bioRxiv - Biochemistry 2024Quote: ... or calpain-3-titin/I81-I83 (2mg/mL) from Superose 6 increase 10/300GL column chromatography was loaded onto glow-discharged holey carbon grids (Quantifoil 1.2/1.3 + 2 nm Carbon). The grids were blotted with filter paper for 2.5 secs at 22 °C and then immediately plunged into liquid ethane cooled by liquid nitrogen using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Supernatant after centrifugation mixed with 10-nm gold beads solution (Aurion, cat # 25486) at 1:1 ratio was added to the mounted grid (Quantifoil R2/2 Cu 200 EM). No other chemicals or stimulation were applied prior to plunge freezing ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μL of ∼120 nM mitoribosome was applied onto a glow-discharged (20 mA for 30 sec) holey-carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ∼3 nm thickness ...
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bioRxiv - Molecular Biology 2022Quote: ... The pellet was then resuspended in warm EM buffer and 3 μL of this sample was applied to a carbon on gold grid (Quantifoil R2/2 Au 200 mesh), recently glow-discharged for 1 min at 30 mA ...
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bioRxiv - Biochemistry 2022Quote: ... The endogenous samples were applied onto grids containing an additional thin layer of continuous carbon support (Quantifoil R1.2/1.3 Cu 200 mesh + 2 nm C) and incubated for 30 sec before blotting and freezing ...