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Citations for G-Biosciences :
101 - 139 of 139 citations for Rat Heat Shock Factor Protein 1 HSF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Particle pellets were resuspended in 1× STE buffer (100 mM NaCl, 10 mM Tris pH 8.0, and 1 mM EDTA) (G-Biosciences, St. Louis, MO). The 293T/17 cells were collected and lysed with RIPA lysis buffer and clarified via centrifugation (1800 × g for 10 min) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA was extracted using an Omniprep kit for genomic DNA (G-Biosciences, St. Louis, MO, USA), following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... the TtArsM arsenite methyltransferase activity was measured using the SAM510: SAM Methyltransferase Assay Kit (G-Biosciences) with modifications regarding the temperature ...
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bioRxiv - Neuroscience 2020Quote: ... LDH levels were measured using a Cytoscan LDH cytotoxicity assay kit (G-Biosciences; Geno Technology Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... SPE Buffer I from the FOCUS™ SubCell kit (# 786-260, G-Biosciences, St Louis, MO) was added to the pellet ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Genomic DNA was extracted using OmniPrep Genomic DNA Extraction Kits (G-Biosciences, St. Louis, MO, USA) and DNeasy Blood and Tissue Kits (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... genomic DNA was extracted using the Omniprep DNA isolation kit (G-Biosciences, St. Louis, MO, USA) from 20 mg of urediniospores of Pca isolate NTU1 ...
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bioRxiv - Genomics 2023Quote: ... we followed the kit standard protocol for Omniprep extraction of genomic DNA (G-Biosciences, St. Louis, MO) and sequenced 250 basepair paired-end reads on an Illumina MiSeq.
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bioRxiv - Neuroscience 2021Quote: ... amplicons were loaded in a 1% low EEO agarose gel (G-BioSciences) and purified using the QIAquick Gel Extraction Kit (QIAGEN ...
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bioRxiv - Systems Biology 2020Quote: ... we treated the 1-mL samples with zymolyase (786-036, G-Biosciences). Zymolyase lysed vegetative cells that formed from germinated spores ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM β-glycerophosphate) using Tube-o-dialyzer 15 kDa (G-Biosciences) at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... in 1×Tris-buffered saline Tween-20 (TBST, G-Biosciences, Cat#R042), and analyzed with the indicated primary antibodies (biotinylated anti-H3K27Me3 (1:1000 ...
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bioRxiv - Microbiology 2022Quote: Detection of SA-β-Gal activity was performed using a colorimetric senescence β-Galactosidase histochemical staining kit (G-Biosciences) or a fluorescent Cell Meter™ Cellular Senescence Activity Assay Kit with Xite™ Red beta-D-galactopyranoside (AAT Bioquest ...
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bioRxiv - Genomics 2022Quote: Genomic DNA was extracted from 30 mg of urediniospores per isolate using the Omniprep DNA isolation kit (G-Biosciences). DNA concentration was determined using Qubit 3.0 Fluorometer (LifeTechnologies ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was prepared from liquid cultures using the XIT Genomic DNA from Gram-Positive Bacteria kit (G-Biosciences), as instructed by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... Samples were dialyzed against water (1 kDa MWCO, G-Biosciences Tube-O-DIALYZER) and stored at -80 deg C prior to inositol quantification by the UCSD GlycoAnalytics Core.
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bioRxiv - Cell Biology 2019Quote: ... 1 mM PMSF and 1x bacterial protease arrest cocktail (G-Biosciences; 786-330) and lysed by sonication (10 sets of 15 pulses on ice with 30 s interval between each set) ...
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bioRxiv - Microbiology 2022Quote: ... 1% NP-40) supplemented with 50 μg/ml phenylmethylsulfonyl fluoride (PMSF; G-Biosciences) and cOmplete protease cocktail inhibitor (Roche) ...
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bioRxiv - Cancer Biology 2021Quote: The F(ab′)2 fragments were generated using trastuzumab and the F(ab′)2 fragmentation kit following the manufacturer’s instructions (G-Biosciences).
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bioRxiv - Cell Biology 2019Quote: ... 1 mg/ml lysozyme and 1x bacterial protease arrest cocktail (G-Biosciences; 786-330) and lysed by sonication (10 sets of 15 pulses on ice with 30 s interval between each set) ...
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bioRxiv - Biochemistry 2021Quote: ... Digestion was done using proteomics grade trypsin 1:20 (G-Biosciences, St. Louis, MO) and a CEM Discover Microwave Reactor (Mathews ...
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bioRxiv - Neuroscience 2023Quote: ... pH=7.5) supplemented with 1:100 proteases inhibitors (G-Biosciences, Cat. no: 786-433) and 1:100 phosphatases inhibitors (Sigma-Adrich ...
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bioRxiv - Genomics 2022Quote: ... DNA was also extracted from 20 mg of isolate Pca203 spores using the Omniprep™ DNA isolation kit from G-Biosciences for library preparation with the Illumina TruSeq Nano DNA protocol and Illumina NovaSeq sequencing in the S2 flow cell at UMGC to produce 150 bp paired-end reads ...
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bioRxiv - Cell Biology 2020Quote: ... Cellular lysates were prepared by using lysis buffer (supplied in RnD Systems Proteome Profiler Human Phosphokinase Array Kit supplemented with 1X “Protease Arrest” and 2X “Phosphatase Arrest” (G-Biosciences)) ...
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bioRxiv - Microbiology 2021Quote: ... sanguinis genomic DNA was prepared from overnight (O/N) liquid cultures using the kit XIT Genomic DNA from Gram-Positive Bacteria (G-Biosciences). Strain 2908 ...
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bioRxiv - Cell Biology 2019Quote: Cells were incubated in serum-free DMEM for 24 h and IL-6/8 levels determined in supernatants using the femtoELISA™ HRP Kit (G-Biosciences).
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bioRxiv - Cell Biology 2023Quote: ... The blots were blocked with 5% BSA in TBST and blotted according to manufacturer’s recommendations for the indicated primary and secondary antibodies (Table S2) followed by development with the femtoLUCENT™ PLUS-HRP Chemiluminescent kit (G-Biosciences) for the anti-GDF3 blot and the SuperSignal™ West Pico Chemiluminescent kit (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... 68 mM NaCl and 1 mM CaCl2) using a Tube-O-Dialyzer Micro (G-Biosciences) with a 50 kDa MWCO ...
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bioRxiv - Genomics 2021Quote: ... and pellets frozen at -80°C. DNA was extracted from pellets (approx. 100 mg) using an OmniPrep kit (G-Biosciences, St. Louis, MO) in 1 ml lysis buffer (G-Biosciences ...
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bioRxiv - Immunology 2020Quote: ... 1% Tx100) supplemented with the protease inhibitor cocktail Protease Arrest (G-Biosciences, St. Louis, MO, USA). Antigen–antibody complexes were captured with Protein G-coupled Dynabeads (Invitrogen ...
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bioRxiv - Evolutionary Biology 2020Quote: Genomic DNA from molecular collection specimens was extracted from the thorax or leg(s) using OmniPrep Genomic DNA Extraction Kits (G-Biosciences, St. Louis, MO, USA) or DNeasy Blood and Tissue Kits (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... Blocks were sectioned and stained with Hematoxylin solution Gill III (MilliporeSigma) and 1% Eosin Y Solution (G-Biosciences). For immunofluorescent staining ...
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bioRxiv - Genetics 2019Quote: ... flanking and internal sequences of the 57 kbp insert in AvrSr35 were amplified from genomic DNA extracted using the OmniPrep™ kit (G-Biosciences, St. Louis, MO, U.S.A.) from rust urediniospores of Ug99 ...
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An engineered receptor-binding domain improves the immunogenicity of multivalent SARS-CoV-2 vaccinesbioRxiv - Microbiology 2020Quote: ... supernatant pH was adjusted to 8.5 by addition of 1/20 volume 1M Tris pH=9.0 (G-Biosciences, 786-476), and the supernatant was filtered by 0.45 μm filter prior to purification on CaptureSelect™ C-tagXL Columns ...
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bioRxiv - Bioengineering 2019Quote: ... pH 8.0; 5 mM MgCl2, 25 mM CaCl2; 50 mM Na2-EDTA, pH 8.0; 1 x ProteaseArrest™ agent, G-Bioscience). Equivalents of each 50 OD units were 5x diluted in thylakoid buffer to a final volume of 1 mL ...
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bioRxiv - Biochemistry 2022Quote: ... The combined supernatants were dialyzed twice against 100 volumes of buffer M containing 1 M KCl (for 6 hours and overnight) using a 1000 DaMWCO Tube-O-Dialyzers (G-Biosciences). The concentration of TP 70 was determined by measuring A230 (1unit A230 = 240pmol TP70) ...
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bioRxiv - Molecular Biology 2023Quote: ... at a ratio of 15:1 (see Supplementary Information, 2. for more details) and 10 µL silica magnetic beads (G-Biosciences, USA). The mixture was briefly vortexed and incubated for 20 minutes at room temperature while shaking at 1000 rpm to facilitate DNA binding ...
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bioRxiv - Genetics 2023Quote: ... Fixed cells were lysed in 3X volume of lysis buffer (1% SDS, 10mM EDTA, 50mM Tris-HCl pH 8.0, 1X protease inhibitor cocktail (G-Biosciences, 786-433). Cells were sonicated for 35 minutes using a Diagenode Bioruptor to shear chromatin to 200-500bp ...
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bioRxiv - Neuroscience 2023Quote: ... by homogenizing the tissue with a 2 cm3 glass-Teflon homogenizer at 800 rpm in lysis buffer (0.32 M sucrose, 5 mM Hepes, and 10 ml ddH2O) supplemented with 1:100 diluted proteases inhibitors (G-Biosciences, Cat. no: 786-433) and 1:100 diluted phosphatases inhibitors (Sigma-Adrich ...