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Citations for G-Biosciences :
51 - 100 of 298 citations for Mouse G Protein Coupled Receptor Family C Group 6 Member A GPRC6A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... The cytoplasmic proteins were also extracted from cells using the Nuclear & Cytoplasmic Extraction Kit (G-Biosciences, St. Louis, MO, USA). The proteins were resolved using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (10% ...
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bioRxiv - Bioengineering 2022Quote: ... Total protein stains were done using Swift Membrane Stain (G-biosciences). The membrane was imaged using a LI-COR Odyssey CLX Imager ...
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bioRxiv - Cell Biology 2022Quote: ... Protein quantification was done using BCA assay (G-Bioscience, 786-570) kit ...
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bioRxiv - Genomics 2021Quote: ... and pellets frozen at -80°C. DNA was extracted from pellets (approx. 100 mg) using an OmniPrep kit (G-Biosciences, St. Louis, MO) in 1 ml lysis buffer (G-Biosciences ...
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bioRxiv - Biophysics 2021Quote: ... the supernatant was affinity purified using Protein A affinity resin (G-Biosciences) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Protein samples were digested in situ using sequencing-grade trypsin (G-Biosciences) at a 1:75 (wt/wt ...
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bioRxiv - Bioengineering 2022Quote: Total protein concentration was determined using BCA methods (G-Biosciences, 786-571) and size distribution as well as particle concentration were assessed using a NanoSight LM10 (Malvern Instruments ...
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bioRxiv - Microbiology 2023Quote: ... purified proteins were mixed with alum adjuvant (G-Bioscience, St. Louis, MO) in a 2:1 ratio of antigen to adjuvant ...
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bioRxiv - Microbiology 2021Quote: ... Supernatants were collected and total protein was normalized using the Non-Interfering Protein Assay (G-Biosciences, St. Louis, MO).
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bioRxiv - Bioengineering 2023Quote: Cytoscan-LDH cytotoxicity assay kit (G-BIOSCIENCES 786-210) was used to measure LDH release from treated spheroids ...
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bioRxiv - Bioengineering 2020Quote: ... XyrA protein was purified using Ni-NTA Resin (G-Biosciences, Cat # 786-939) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... Total protein was assessed via BLOT-FastStain (G-Bioscience, St. Louis, MO, USA) and used as the loading control ...
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bioRxiv - Physiology 2023Quote: ... Total protein was measured via BLOT-FastStain (G-Bioscience, St. Louis, MO, USA) and used as a loading control ...
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bioRxiv - Biochemistry 2023Quote: ... C-His tagged IDH1 R132H (Met1-Leu414) was purchased from G-Biosciences (BAN1708, 50 µg). N-His and GST tagged USP7 (Lys208-Glu560 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We assessed overall protein concentration in the upper fraction of the solution using RED 660™ protein assay (G-Biosciences) measured in triplicates and then diluted the sample to 0.45 mg/mL ...
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bioRxiv - Molecular Biology 2020Quote: ... Nickel NTA immobilized metal affinity chromatography protein purification beads were purchased from G-BioSciences. Ionomycin and tricaine methanesulfonate were purchased from Millipore-Sigma ...
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bioRxiv - Microbiology 2022Quote: ... The wells were blocked with 1:1 Non-Animal Protein-BLOCKER™ (G-Biosciences) in TBS for 1 hour ...
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Low spontaneous mutation rate in a complex multicellular eukaryote with a haploid-diploid life cyclebioRxiv - Evolutionary Biology 2022Quote: ... DNA was extracted using the OmniPrep Plant kit (G-Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Crosslinks on nucleosomes were reversed at 65 °C in the presence of Proteinase K (G-Biosciences) overnight ...
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bioRxiv - Immunology 2021Quote: ... 1:1 NAP-Blocker (NAP [Non Animal Protein]-BLOCKER™, G-Biosciences cat # 786-190) in TBS was added for 1 hour at 37°C ...
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bioRxiv - Immunology 2021Quote: ... 150 µl blocking buffer (one-third Non-Animal Protein (NAP)-Blocker (G-Biosciences #786-190P) and two-thirds PBS ...
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bioRxiv - Plant Biology 2021Quote: ... The VirE2 protein was detected by the anti-VirE2 polyclonal antibody (G-Biosciences, 1:10000) and anti-rabbit secondary antibody (Sigma ...
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bioRxiv - Immunology 2023Quote: ... 150 µl blocking buffer (one-third Non-Animal Protein (NAP)-Blocker (G-Biosciences #786-190P) and two-thirds PBS ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein sample was then transferred to midi-size 1 kDa MWCO Tube-O-Dialyzers (G-Biosciences) and first dialyzed against 100 volumes of simplified high-salt buffer with urea overnight ...
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bioRxiv - Plant Biology 2021Quote: ... The VirE2 protein was detected by anti-VirE2 polyclonal primary antibody (G-Biosciences, 1:10 000) and anti-rabbit secondary antibody (Sigma ...
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bioRxiv - Plant Biology 2023Quote: The purification of recombinant protein was carried out on a Ni-NTA column (G-Biosciences, USA). One ml of Ni-NTA Sefinose resin was loaded onto a plastic column ...
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bioRxiv - Plant Biology 2021Quote: ... was transformed using the Fast Yeast Transformation kit (G-Biosciences, MO, USA). All transformants were selected on a yeast minimal medium without tryptophan ...
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bioRxiv - Genetics 2023Quote: ... coli prepared using Z-Competent cell kit (G-Biosciences, St. Louis, MO) and plated to achieve approximately 104 colonies per plate ...
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bioRxiv - Biochemistry 2022Quote: ... The combined supernatants were dialyzed twice against 100 volumes of buffer M containing 1 M KCl (for 6 hours and overnight) using a 1000 DaMWCO Tube-O-Dialyzers (G-Biosciences). The concentration of TP 70 was determined by measuring A230 (1unit A230 = 240pmol TP70) ...
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bioRxiv - Bioengineering 2023Quote: ... Total protein concentration in BEV samples was determined using Bicinchoninic acid assay (BCA; 786-571; G-Biosciences). AEC samples were buffer exchanged with 1× PBS prior to BCA to remove excess NaCl and adjust pH to 7.4.
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bioRxiv - Immunology 2024Quote: ... One hundred fifty µL blocking buffer (one-third Non-Animal Protein (NAP)-Blocker (G-Biosciences #786-190P) and two-thirds PBS ...
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bioRxiv - Microbiology 2022Quote: His-FhaAMtb was induced with 1mM IPTG at 18°C overnight and purified on Ni-NTA resin (G-Biosciences, 786-940 in 5 ml Bio-Scale Mini Cartridges ...
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bioRxiv - Cell Biology 2022Quote: ... The plates were incubated for two days at 30 □C before the X-Gal (#RC-212, G-Biosciences, USA) overlay ...
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bioRxiv - Microbiology 2020Quote: ... and PDGF-D by enzyme-linked immunosorbent assay (ELISA, TGF-β: Thermo Fisher Scientific, Waltham, MA; CTGF and PDGF-D: G-Biosciences, St. Louis, MO; Abclonal, Woburn, MA) as previously reported and the data from these prior experiments was used in the current investigations.22 Each experiment was performed with two technical replicates.
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bioRxiv - Plant Biology 2022Quote: ... after inducing expression with 1 mM IPTG for 16 h at 20°C and purified by cobalt-nitrilotriacetic acid affinity chromatography according to the manufacturer’s instructions (G-Biosciences), including a washing step with 5 mM Mg-ATP ...
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bioRxiv - Plant Biology 2022Quote: ... coli Rosetta cells after induction with 1 mM IPTG for 16 h at 20°C and purified by cobalt-nitrilotriacetic acid affinity chromatography according to the manufacturer’s instructions (G-Biosciences), including a washing step with 5 mM ATP ...
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bioRxiv - Biochemistry 2022Quote: ... Visualization of HRP was accomplished by colorimetric detection (Femto-Chromo HRP kit, G-Biosciences).
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bioRxiv - Genetics 2019Quote: ... 60 and 61 using the OmniPrep™ kit (G-Biosciences, St. Louis, MO, U.S.A.) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Yeast transformation was performed using the Fast™ yeast transformation kit (G-Biosciences, USA) to integrate the linear pAUR101 vector ...
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bioRxiv - Bioengineering 2022Quote: ... T7 RNA polymerase promoter was used for the overexpression of mutant protein by IPTG (G-Biosciences, St. Louis, USA) induction ...
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bioRxiv - Developmental Biology 2023Quote: ... Sp1 (G-Biosciences)].
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA was extracted using an OmniPrep Genomic DNA Extraction Kit (G-Biosciences, St. Louis, MO), following the protocol of Espeland et al ...
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bioRxiv - Molecular Biology 2019Quote: ... The samples were pre-treated as per the kit protocol (G-Biosciences, St.Louis, Missouri, USA) and incubated in specific antibody pre-coated wells ...
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bioRxiv - Cancer Biology 2019Quote: ... A non-radioactive colorimetric continuous enzyme kit (#786-430; G-Biosciences, St. Louis, MO, USA) was used to measure the enzyme activity of METTL18 according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The concentration of the protein was done using Bradford assay (Puregene PG-035-500mL) or BCA assay (G-Biosciences-786570). For western blotting 25 ug protein was loaded in each well ...
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bioRxiv - Microbiology 2023Quote: ... The protein concentration was determined using a Bradford assay (47) with bovine serum albumin (BSA) as the standard (G-Biosciences, Geno technology inc. ...
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bioRxiv - Immunology 2021Quote: ... the cells were induced with IPTG overnight at 18°C followed by sonication and purification using Ni-NTA resin (Cat. No # 786-940, G-Biosciences, USA). The purity was confirmed by SDS-PAGE and the quantity was measured using NanoDrop 2000 (Thermo Fischer Scientific ...
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bioRxiv - Immunology 2020Quote: ... G-capsule for electroelution (#786-001) was purchased from G-Biosciences and Freeze ‘N Squeeze DNA gel extraction columns by Bio-rad ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA was extracted using an Omniprep kit for genomic DNA (G-Biosciences, St. Louis, MO, USA), following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... the TtArsM arsenite methyltransferase activity was measured using the SAM510: SAM Methyltransferase Assay Kit (G-Biosciences) with modifications regarding the temperature ...