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Citations for G-Biosciences :
51 - 100 of 105 citations for Heat Shock Factor Protein 1 HSF1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... nucleoporins were normalized to total protein levels using the BLOT-FastStain Kit (G-Biosciences).
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bioRxiv - Biochemistry 2019Quote: ... and total protein estimation was done using BCA assay (BCA assay kit, G-Biosciences). Samples were normalized (to maintain constant protein amounts ...
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bioRxiv - Immunology 2021Quote: ... 150 µl blocking buffer (one-third Non-Animal Protein (NAP)-Blocker (G-Biosciences #786-190P) and two-thirds PBS ...
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bioRxiv - Immunology 2023Quote: ... 150 µl blocking buffer (one-third Non-Animal Protein (NAP)-Blocker (G-Biosciences #786-190P) and two-thirds PBS ...
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bioRxiv - Plant Biology 2023Quote: The purification of recombinant protein was carried out on a Ni-NTA column (G-Biosciences, USA). One ml of Ni-NTA Sefinose resin was loaded onto a plastic column ...
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bioRxiv - Bioengineering 2023Quote: ... Total protein concentration in BEV samples was determined using Bicinchoninic acid assay (BCA; 786-571; G-Biosciences). AEC samples were buffer exchanged with 1× PBS prior to BCA to remove excess NaCl and adjust pH to 7.4.
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bioRxiv - Immunology 2024Quote: ... One hundred fifty µL blocking buffer (one-third Non-Animal Protein (NAP)-Blocker (G-Biosciences #786-190P) and two-thirds PBS ...
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bioRxiv - Immunology 2022Quote: ... The soluble recombinant E protein was recovered by 6-HIS affinity chromatography on Ni-NTA agarose (G-Biosciences) and purified by size exclusion chromatography on a Superdex S200 Increase column (Cytiva).
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bioRxiv - Evolutionary Biology 2020Quote: ... The protein concentration of this pooled tapeworm homogenate solution was measured using a Red 660 kit (G-Biosciences), and then diluted to 1mg/ml using 0.9× pbs ...
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bioRxiv - Molecular Biology 2022Quote: ... Primary antibodies were incubated in 5% BLOT-QuickBlocker (G-Biosciences 786-011) as follows ...
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bioRxiv - Molecular Biology 2020Quote: ... Secondary antibodies were incubated in 5% BLOT-QuickBlocker (G-Biosciences 786-011) at 1:10,000 dilutions ...
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bioRxiv - Molecular Biology 2020Quote: ... Primary antibodies were incubated in 5% BLOT-QuickBlocker (G-Biosciences 786-011) as follows ...
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bioRxiv - Bioengineering 2022Quote: ... T7 RNA polymerase promoter was used for the overexpression of mutant protein by IPTG (G-Biosciences, St. Louis, USA) induction ...
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bioRxiv - Cancer Biology 2019Quote: ... The cytoplasmic proteins were also extracted from cells using the Nuclear & Cytoplasmic Extraction Kit (G-Biosciences, St. Louis, MO, USA). The proteins were resolved using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (10% ...
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bioRxiv - Biochemistry 2023Quote: ... The concentration of the protein was done using Bradford assay (Puregene PG-035-500mL) or BCA assay (G-Biosciences-786570). For western blotting 25 ug protein was loaded in each well ...
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bioRxiv - Microbiology 2023Quote: ... The protein concentration was determined using a Bradford assay (47) with bovine serum albumin (BSA) as the standard (G-Biosciences, Geno technology inc. ...
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bioRxiv - Pathology 2023Quote: Aortic samples were homogenized in Cell Lysis buffer and aortic protein (10 µg) was dissolved in zymogram sample buffer (786-483, G-Biosciences). Subsequently ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μM dexamethasone (G-Biosciences), 0.5 mM methylisobutylxanthine ...
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bioRxiv - Microbiology 2024Quote: ... 1% bromophenol blue (G-Biosciences)) ...
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bioRxiv - Neuroscience 2021Quote: ... AceTau (1:500, #ITK0084, G-Biosciences), MC1 (1:500 ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mM PMSF (G-Biosciences, #786), and 0.1% Triton X-100 (AMRESCO ...
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bioRxiv - Biochemistry 2022Quote: ... A final concentration of 2 mM CaCl2 was added to the protein eluate prior to incubation with Calmodulin agarose beads (G-Biosciences; Cat# 786-282) for 90 min at 4 °C ...
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bioRxiv - Synthetic Biology 2022Quote: ... and the supernatant was mixed in a 1:1 ratio with HIS-Binding/Wash Buffer (G-Biosciences, USA) and 50 µl HisTag Dynabeads (Thermo Fischer Scientific ...
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bioRxiv - Genetics 2022Quote: ... + 1 μM dexamethasone (G-bioscience, API-04) + 0.5 mM isobutylmethylxanthine (Sigma ...
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bioRxiv - Genomics 2021Quote: ... in 1 ml lysis buffer (G-Biosciences) with 10 µl proteinase K and digested for 1 hour at 60°C with periodic mixing to keep spores suspended in the lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... supplemented with 1 × Mammalian ProteaseArrest (G-Biosciences), 1 mM phenylmethane sulfonyl fluoride ...
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bioRxiv - Neuroscience 2023Quote: ... and 1 mM PMSF (G-Biosciences, 82021). The cell lysates were clarified by centrifugation at 13,000 g at 4°C for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: ... Particle pellets were resuspended in 1× STE buffer (100 mM NaCl, 10 mM Tris pH 8.0, and 1 mM EDTA) (G-Biosciences, MO). The 293T/17 cells were collected and lysed with RIPA lysis buffer and clarified via centrifugation (1800 x g for 10 min) ...
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bioRxiv - Bioengineering 2019Quote: ... A polyclonal anti-elastin antibody (custom-made at Clemson University) was thiolated with Traut’s reagent (G-Biosciences, Saint Louis, MO) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 1 mM Phenylmethylsulfonyl fluoride (PMSF) (786-055, G-Biosciences) for 10 min on ice ...
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bioRxiv - Microbiology 2023Quote: ... 1 ml of nickel-chelating resin slurry (G-Biosciences) was added to the cleared lysate ...
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bioRxiv - Molecular Biology 2024Quote: ... Particle pellets were resuspended in 1× STE buffer (100 mM NaCl, 10 mM Tris pH 8.0, and 1 mM EDTA) (G-Biosciences, St. Louis, MO). The 293T/17 cells were collected and lysed with RIPA lysis buffer and clarified via centrifugation (1800 × g for 10 min) ...
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bioRxiv - Cell Biology 2023Quote: ... The blots were blocked with 5% BSA in TBST and blotted according to manufacturer’s recommendations for the indicated primary and secondary antibodies (Table S2) followed by development with the femtoLUCENT™ PLUS-HRP Chemiluminescent kit (G-Biosciences) for the anti-GDF3 blot and the SuperSignal™ West Pico Chemiluminescent kit (Thermo Fisher ...
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bioRxiv - Immunology 2019Quote: ... whole cell extracts were prepared as previously described (57) and analyzed with the following antibodies in 5% BLOT-QuickBlocker (G-Biosciences 786-011) in TBST ...
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bioRxiv - Immunology 2019Quote: ... whole cell extracts were prepared as previously described (57) and analyzed with the following antibodies in 5% BLOT-QuickBlocker (G-Biosciences 786-011) in TBST ...
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bioRxiv - Neuroscience 2021Quote: ... amplicons were loaded in a 1% low EEO agarose gel (G-BioSciences) and purified using the QIAquick Gel Extraction Kit (QIAGEN ...
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bioRxiv - Systems Biology 2020Quote: ... we treated the 1-mL samples with zymolyase (786-036, G-Biosciences). Zymolyase lysed vegetative cells that formed from germinated spores ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM β-glycerophosphate) using Tube-o-dialyzer 15 kDa (G-Biosciences) at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... in 1×Tris-buffered saline Tween-20 (TBST, G-Biosciences, Cat#R042), and analyzed with the indicated primary antibodies (biotinylated anti-H3K27Me3 (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... Samples were dialyzed against water (1 kDa MWCO, G-Biosciences Tube-O-DIALYZER) and stored at -80 deg C prior to inositol quantification by the UCSD GlycoAnalytics Core.
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bioRxiv - Cell Biology 2019Quote: ... 1 mM PMSF and 1x bacterial protease arrest cocktail (G-Biosciences; 786-330) and lysed by sonication (10 sets of 15 pulses on ice with 30 s interval between each set) ...
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bioRxiv - Microbiology 2022Quote: ... 1% NP-40) supplemented with 50 μg/ml phenylmethylsulfonyl fluoride (PMSF; G-Biosciences) and cOmplete protease cocktail inhibitor (Roche) ...
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bioRxiv - Cell Biology 2019Quote: ... 1 mg/ml lysozyme and 1x bacterial protease arrest cocktail (G-Biosciences; 786-330) and lysed by sonication (10 sets of 15 pulses on ice with 30 s interval between each set) ...
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bioRxiv - Biochemistry 2021Quote: ... Digestion was done using proteomics grade trypsin 1:20 (G-Biosciences, St. Louis, MO) and a CEM Discover Microwave Reactor (Mathews ...
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bioRxiv - Neuroscience 2023Quote: ... pH=7.5) supplemented with 1:100 proteases inhibitors (G-Biosciences, Cat. no: 786-433) and 1:100 phosphatases inhibitors (Sigma-Adrich ...
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bioRxiv - Microbiology 2020Quote: ... 68 mM NaCl and 1 mM CaCl2) using a Tube-O-Dialyzer Micro (G-Biosciences) with a 50 kDa MWCO ...
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bioRxiv - Immunology 2020Quote: ... 1% Tx100) supplemented with the protease inhibitor cocktail Protease Arrest (G-Biosciences, St. Louis, MO, USA). Antigen–antibody complexes were captured with Protein G-coupled Dynabeads (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... albicans was performed according to the protocol of the Fast Yeast Transformation Kit (G-Biosciences, Cat. GZ-1). Gene deletion was verified by colony PCR as described [24] ...
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bioRxiv - Immunology 2022Quote: ... Blocks were sectioned and stained with Hematoxylin solution Gill III (MilliporeSigma) and 1% Eosin Y Solution (G-Biosciences). For immunofluorescent staining ...
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An engineered receptor-binding domain improves the immunogenicity of multivalent SARS-CoV-2 vaccinesbioRxiv - Microbiology 2020Quote: ... supernatant pH was adjusted to 8.5 by addition of 1/20 volume 1M Tris pH=9.0 (G-Biosciences, 786-476), and the supernatant was filtered by 0.45 μm filter prior to purification on CaptureSelect™ C-tagXL Columns ...