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Citations for G-Biosciences :
251 - 298 of 298 citations for 6H Pyrano 3 2 g benzothiazole 8CI 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and the protein concentration of the samples was measured by Micro Bicinchoninic Acid Protein Assay kit (G-Biosciences; #786-572). 2-mercaptoethanol was added to a final concentration of 1% (v/v) ...
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bioRxiv - Cell Biology 2022Quote: Mitochondrial ferritin in HCEC-B4G12 and F35T cells were quantified using Immunotag™ Mitochondrial Ferritin ELISA kit (G-Biosciences, USA). Protein was extracted following the same protocol mentioned above in cytosolic ferritin ELISA ...
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An engineered receptor-binding domain improves the immunogenicity of multivalent SARS-CoV-2 vaccinesbioRxiv - Microbiology 2020Quote: ... supernatant pH was adjusted to 8.5 by addition of 1/20 volume 1M Tris pH=9.0 (G-Biosciences, 786-476), and the supernatant was filtered by 0.45 μm filter prior to purification on CaptureSelect™ C-tagXL Columns ...
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bioRxiv - Bioengineering 2019Quote: ... pH 8.0; 5 mM MgCl2, 25 mM CaCl2; 50 mM Na2-EDTA, pH 8.0; 1 x ProteaseArrest™ agent, G-Bioscience). Equivalents of each 50 OD units were 5x diluted in thylakoid buffer to a final volume of 1 mL ...
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bioRxiv - Plant Biology 2022Quote: ... after inducing expression with 1 mM IPTG for 16 h at 20°C and purified by cobalt-nitrilotriacetic acid affinity chromatography according to the manufacturer’s instructions (G-Biosciences), including a washing step with 5 mM Mg-ATP ...
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bioRxiv - Plant Biology 2022Quote: ... coli Rosetta cells after induction with 1 mM IPTG for 16 h at 20°C and purified by cobalt-nitrilotriacetic acid affinity chromatography according to the manufacturer’s instructions (G-Biosciences), including a washing step with 5 mM ATP ...
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bioRxiv - Biochemistry 2023Quote: ... The concentration of the protein was done using Bradford assay (Puregene PG-035-500mL) or BCA assay (G-Biosciences-786570). For western blotting 25 ug protein was loaded in each well ...
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bioRxiv - Neuroscience 2023Quote: Media samples were collected from individual hCO wells and run using the Cytoscan LDH Cytotoxicity Assay (G-Biosciences, 786-210) per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... frozen tumor tissues were crushed to powder and lysed with urea lysis buffer containing 8 M urea (G-Biosciences #BC89), 75 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... The protein concentration was determined using a Bradford assay (47) with bovine serum albumin (BSA) as the standard (G-Biosciences, Geno technology inc. ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were then sonicated and cell lysate containing GST tagged anti-GFP nanobody was incubated with glutathione resin (G-Biosciences, U.S.A.) at 4°C for 1 h ...
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bioRxiv - Plant Biology 2020Quote: ... All Ulva gene and promoter sequences were cloned from genomic DNA, isolated via CTAB (Clarke, 2009) method or Omniprep for plant (G-Biosciences).
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bioRxiv - Molecular Biology 2021Quote: ... Beads were washed three times in 1X TESH buffer with 0.15 M NaCl and resuspended in 40 µl of RIPA buffer (G-Biosciences, MO) on ice for 1 hour ...
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bioRxiv - Microbiology 2022Quote: ... Particle pellets were resuspended in 1× STE buffer (100 mM NaCl, 10 mM Tris pH 8.0, and 1 mM EDTA) (G-Biosciences, MO). The 293T/17 cells were collected and lysed with RIPA lysis buffer and clarified via centrifugation (1800 x g for 10 min) ...
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bioRxiv - Genomics 2022Quote: ... DNA was also extracted from 20 mg of isolate Pca203 spores using the Omniprep™ DNA isolation kit from G-Biosciences for library preparation with the Illumina TruSeq Nano DNA protocol and Illumina NovaSeq sequencing in the S2 flow cell at UMGC to produce 150 bp paired-end reads ...
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bioRxiv - Physiology 2022Quote: ... Levels of TSH and GH were determined by enzyme-linked immunoassay (ELISA) using commercially available ELISA kits (TSH ELISA kit, G-Biosciences, Cat No ...
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bioRxiv - Cell Biology 2020Quote: ... Cellular lysates were prepared by using lysis buffer (supplied in RnD Systems Proteome Profiler Human Phosphokinase Array Kit supplemented with 1X “Protease Arrest” and 2X “Phosphatase Arrest” (G-Biosciences)) ...
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bioRxiv - Microbiology 2021Quote: ... sanguinis genomic DNA was prepared from overnight (O/N) liquid cultures using the kit XIT Genomic DNA from Gram-Positive Bacteria (G-Biosciences). Strain 2908 ...
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bioRxiv - Biochemistry 2022Quote: ... The combined supernatants were dialyzed twice against 100 volumes of buffer M containing 1 M KCl (for 6 hours and overnight) using a 1000 DaMWCO Tube-O-Dialyzers (G-Biosciences). The concentration of TP 70 was determined by measuring A230 (1unit A230 = 240pmol TP70) ...
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bioRxiv - Physiology 2023Quote: ... TEV protease and any free MBP or uncleaved MBP fusion were further removed using a cobalt resin (Catalog 786-402, G-Biosciences).
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bioRxiv - Pathology 2023Quote: Aortic samples were homogenized in Cell Lysis buffer and aortic protein (10 µg) was dissolved in zymogram sample buffer (786-483, G-Biosciences). Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... transcripts were detected by gene-specific 32P-labeled oligonucleotides (Supplementary Table S1) in hybridization buffer (G-Biosciences, Saint Louis, MI, USA) and exposed to a phosphor screen overnight ...
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bioRxiv - Immunology 2021Quote: ... the cells were induced with IPTG overnight at 18°C followed by sonication and purification using Ni-NTA resin (Cat. No # 786-940, G-Biosciences, USA). The purity was confirmed by SDS-PAGE and the quantity was measured using NanoDrop 2000 (Thermo Fischer Scientific ...
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bioRxiv - Cell Biology 2019Quote: Cells were incubated in serum-free DMEM for 24 h and IL-6/8 levels determined in supernatants using the femtoELISA™ HRP Kit (G-Biosciences).
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bioRxiv - Biochemistry 2021Quote: ... The detergent present in the incubation mixture with BPS was removed using a DetergentOUT™ spin column (G-Biosciences, St Louis, MO). The protein was concentrated to 25-30 µM with the use of a Centrisart I MWCO 100 kDa concentrator (Sartorius AG ...
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bioRxiv - Genomics 2019Quote: ... Red blood cells were first removed with the addition of red blood cell (RBC) lysis buffer (G-Bioscience, St. Louis, MO, USA) and incubation for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... and smaller organs such as the heart and intestine were placed in 1.5 ml of lysis buffer (Tissue-PE LBTM, G-Biosciences #786-181). These organs were then frozen in liquid nitrogen and kept at −80°C for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1.6 µL 1X TE Buffer (pH 8.0, Low EDTA (Tris-EDTA; 10 mM Tris base, 0.1 mM EDTA)) (PN 786-150, G-Biosciences, St. Louis, MO). Each dPCR reaction mix was then vortexed three times for five second pulses ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were blocked for >60 min at room temperature with gentle shaking in 5% BLOT-QuickBlocker (w/v) (G-Bioscience 786-011) in phosphate buffered saline with 0.05% Tween-20 v/v (PBS-T).
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bioRxiv - Cell Biology 2023Quote: ... The blots were blocked with 5% BSA in TBST and blotted according to manufacturer’s recommendations for the indicated primary and secondary antibodies (Table S2) followed by development with the femtoLUCENT™ PLUS-HRP Chemiluminescent kit (G-Biosciences) for the anti-GDF3 blot and the SuperSignal™ West Pico Chemiluminescent kit (Thermo Fisher ...
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bioRxiv - Genetics 2023Quote: ... Fixed cells were lysed in 3X volume of lysis buffer (1% SDS, 10mM EDTA, 50mM Tris-HCl pH 8.0, 1X protease inhibitor cocktail (G-Biosciences, 786-433). Cells were sonicated for 35 minutes using a Diagenode Bioruptor to shear chromatin to 200-500bp ...
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bioRxiv - Physiology 2021Quote: ... gels were rinsed in diH2O for 15 minutes and immersed in Coomassie stain (Lab Safe GEL Blue; G-Biosciences; St. Louis, MO, USA) for 2 hours ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the cytotoxicity of extracts and pure compounds was determined according to the manufacturer's instructions using the CytoScan LDH cytotoxicity assay (G-Biosciences, St. Louis, MO). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... The resin was then resuspended in 5 ml of Buffer A and transferred to a 5-ml gravity column (G-Biosciences, MO, USA). The resin was further washed with 20 ml of Buffer A ...
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bioRxiv - Immunology 2019Quote: ... whole cell extracts were prepared as previously described (57) and analyzed with the following antibodies in 5% BLOT-QuickBlocker (G-Biosciences 786-011) in TBST ...
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bioRxiv - Immunology 2019Quote: ... whole cell extracts were prepared as previously described (57) and analyzed with the following antibodies in 5% BLOT-QuickBlocker (G-Biosciences 786-011) in TBST ...
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bioRxiv - Genomics 2021Quote: ... and pellets frozen at -80°C. DNA was extracted from pellets (approx. 100 mg) using an OmniPrep kit (G-Biosciences, St. Louis, MO) in 1 ml lysis buffer (G-Biosciences ...
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bioRxiv - Genomics 2021Quote: ... filter cuttings contained in the 2 ml Lysing Matrix E tubes were submerged in 294 µl 10X Tris-EDTA (100 mM Tris, 10 mM EDTA, pH 8.0, G-Biosciences, Cat. No: 501035446) and 6 μl lysozyme solution (50 mg.ml-1 ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were then spun for 10 min at 6000 rpm at 4 °C and pre-cleared by incubating them under rotation with 25 μl of Sepharose CL-4B beads (G-Biosciences, #786-1560) for further 30 min at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... Particle pellets were resuspended in 1× STE buffer (100 mM NaCl, 10 mM Tris pH 8.0, and 1 mM EDTA) (G-Biosciences, St. Louis, MO). The 293T/17 cells were collected and lysed with RIPA lysis buffer and clarified via centrifugation (1800 × g for 10 min) ...
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bioRxiv - Bioengineering 2020Quote: Actinobacillus succinogenes 130Z (ATCC 55618) was obtained from the American Type Culture Collection (Manassas, VA) and maintained in tryptic soy broth media (G-Biosciences, St. Louis, MO) with 10 g/L glucose (Fisher Chemical ...
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bioRxiv - Neuroscience 2023Quote: ... by homogenizing the tissue with a 2 cm3 glass-Teflon homogenizer at 800 rpm in lysis buffer (0.32 M sucrose, 5 mM Hepes, and 10 ml ddH2O) supplemented with 1:100 diluted proteases inhibitors (G-Biosciences, Cat. no: 786-433) and 1:100 diluted phosphatases inhibitors (Sigma-Adrich ...
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bioRxiv - Bioengineering 2023Quote: ... Protein concentration of EV samples was determined using a bicinchoninic acid (BCA) assay using the manufacturer’s protocol (785-571; G-Biosciences, St. Louis, MO, USA). Equal amounts of protein of EV or lysate samples were then subjected to western blot analysis for ALIX (ab186429 ...
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bioRxiv - Evolutionary Biology 2020Quote: Genomic DNA from molecular collection specimens was extracted from the thorax or leg(s) using OmniPrep Genomic DNA Extraction Kits (G-Biosciences, St. Louis, MO, USA) or DNeasy Blood and Tissue Kits (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: ... All surfaces for tissue collection and processing were sanitized using 70% ethanol and then treated with an RNAse inhibitor (RNAse out, G-Biosciences, St. Louis, MO, USA) to maintain integrity of isolated RNA ...
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bioRxiv - Cancer Biology 2024Quote: ... The reaction was allowed to proceed for 20 min at 56°C and alkylated for 30 min in the dark with 10 mM iodoacetamide at room temperature (G-Biosciences, St. Louis, MO, USA). Proteins were precipitated at –20°C with ethanol ...
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bioRxiv - Genetics 2019Quote: ... flanking and internal sequences of the 57 kbp insert in AvrSr35 were amplified from genomic DNA extracted using the OmniPrep™ kit (G-Biosciences, St. Louis, MO, U.S.A.) from rust urediniospores of Ug99 ...
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bioRxiv - Microbiology 2020Quote: ... and PDGF-D by enzyme-linked immunosorbent assay (ELISA, TGF-β: Thermo Fisher Scientific, Waltham, MA; CTGF and PDGF-D: G-Biosciences, St. Louis, MO; Abclonal, Woburn, MA) as previously reported and the data from these prior experiments was used in the current investigations.22 Each experiment was performed with two technical replicates.