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Citations for G-Biosciences :
51 - 100 of 139 citations for Rat Heat Shock Factor Protein 1 HSF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... 100ul of Protein A agarose beads (G-Bioscience, 786-283) were added to the mixture of chromatin and antibody and incubated for 4-6 hours at 40C ...
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bioRxiv - Cell Biology 2020Quote: ... and Protein G resin (G-Biosciences, St. Louis, MO, USA) prewashed with lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were then transferred to polyvinylidene difluoride (PVDF, G-Biosciences) membranes by wet blotting using standard methods ...
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bioRxiv - Microbiology 2020Quote: ... and PDGF-D by enzyme-linked immunosorbent assay (ELISA, TGF-β: Thermo Fisher Scientific, Waltham, MA; CTGF and PDGF-D: G-Biosciences, St. Louis, MO; Abclonal, Woburn, MA) as previously reported and the data from these prior experiments was used in the current investigations.22 Each experiment was performed with two technical replicates.
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bioRxiv - Bioengineering 2022Quote: ... Total protein stains were done using Swift Membrane Stain (G-biosciences). The membrane was imaged using a LI-COR Odyssey CLX Imager ...
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bioRxiv - Cell Biology 2022Quote: ... Protein quantification was done using BCA assay (G-Bioscience, 786-570) kit ...
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bioRxiv - Biophysics 2021Quote: ... the supernatant was affinity purified using Protein A affinity resin (G-Biosciences) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Protein samples were digested in situ using sequencing-grade trypsin (G-Biosciences) at a 1:75 (wt/wt ...
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bioRxiv - Bioengineering 2022Quote: Total protein concentration was determined using BCA methods (G-Biosciences, 786-571) and size distribution as well as particle concentration were assessed using a NanoSight LM10 (Malvern Instruments ...
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bioRxiv - Microbiology 2023Quote: ... purified proteins were mixed with alum adjuvant (G-Bioscience, St. Louis, MO) in a 2:1 ratio of antigen to adjuvant ...
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bioRxiv - Bioengineering 2020Quote: ... XyrA protein was purified using Ni-NTA Resin (G-Biosciences, Cat # 786-939) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... Total protein was assessed via BLOT-FastStain (G-Bioscience, St. Louis, MO, USA) and used as the loading control ...
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bioRxiv - Physiology 2023Quote: ... Total protein was measured via BLOT-FastStain (G-Bioscience, St. Louis, MO, USA) and used as a loading control ...
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bioRxiv - Molecular Biology 2020Quote: ... Nickel NTA immobilized metal affinity chromatography protein purification beads were purchased from G-BioSciences. Ionomycin and tricaine methanesulfonate were purchased from Millipore-Sigma ...
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bioRxiv - Immunology 2021Quote: ... 150 µl blocking buffer (one-third Non-Animal Protein (NAP)-Blocker (G-Biosciences #786-190P) and two-thirds PBS ...
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bioRxiv - Immunology 2023Quote: ... 150 µl blocking buffer (one-third Non-Animal Protein (NAP)-Blocker (G-Biosciences #786-190P) and two-thirds PBS ...
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bioRxiv - Plant Biology 2023Quote: The purification of recombinant protein was carried out on a Ni-NTA column (G-Biosciences, USA). One ml of Ni-NTA Sefinose resin was loaded onto a plastic column ...
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bioRxiv - Bioengineering 2023Quote: ... Total protein concentration in BEV samples was determined using Bicinchoninic acid assay (BCA; 786-571; G-Biosciences). AEC samples were buffer exchanged with 1× PBS prior to BCA to remove excess NaCl and adjust pH to 7.4.
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bioRxiv - Immunology 2024Quote: ... One hundred fifty µL blocking buffer (one-third Non-Animal Protein (NAP)-Blocker (G-Biosciences #786-190P) and two-thirds PBS ...
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bioRxiv - Plant Biology 2020Quote: ... EZ Yeast plasmid preparation kit (G-Bioscience) was used to isolate the plasmids from these colonies ...
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bioRxiv - Microbiology 2024Quote: ... yeast transformation kit from G-Biosciences (USA), trizol reagent and DMEM from Gibco (USA) ...
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bioRxiv - Immunology 2022Quote: ... The soluble recombinant E protein was recovered by 6-HIS affinity chromatography on Ni-NTA agarose (G-Biosciences) and purified by size exclusion chromatography on a Superdex S200 Increase column (Cytiva).
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bioRxiv - Evolutionary Biology 2024Quote: ... using the G-Biosciences OmniPrep kit (G-Biosciences, Catalog #786-136 ...
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bioRxiv - Bioengineering 2022Quote: ... T7 RNA polymerase promoter was used for the overexpression of mutant protein by IPTG (G-Biosciences, St. Louis, USA) induction ...
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bioRxiv - Bioengineering 2023Quote: Cytoscan-LDH cytotoxicity assay kit (G-BIOSCIENCES 786-210) was used to measure LDH release from treated spheroids ...
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bioRxiv - Biochemistry 2023Quote: ... The concentration of the protein was done using Bradford assay (Puregene PG-035-500mL) or BCA assay (G-Biosciences-786570). For western blotting 25 ug protein was loaded in each well ...
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bioRxiv - Microbiology 2023Quote: ... The protein concentration was determined using a Bradford assay (47) with bovine serum albumin (BSA) as the standard (G-Biosciences, Geno technology inc. ...
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bioRxiv - Pathology 2023Quote: Aortic samples were homogenized in Cell Lysis buffer and aortic protein (10 µg) was dissolved in zymogram sample buffer (786-483, G-Biosciences). Subsequently ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μM dexamethasone (G-Biosciences), 0.5 mM methylisobutylxanthine ...
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bioRxiv - Microbiology 2024Quote: ... 1% bromophenol blue (G-Biosciences)) ...
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Low spontaneous mutation rate in a complex multicellular eukaryote with a haploid-diploid life cyclebioRxiv - Evolutionary Biology 2022Quote: ... DNA was extracted using the OmniPrep Plant kit (G-Biosciences) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... AceTau (1:500, #ITK0084, G-Biosciences), MC1 (1:500 ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mM PMSF (G-Biosciences, #786), and 0.1% Triton X-100 (AMRESCO ...
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bioRxiv - Biochemistry 2022Quote: ... A final concentration of 2 mM CaCl2 was added to the protein eluate prior to incubation with Calmodulin agarose beads (G-Biosciences; Cat# 786-282) for 90 min at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... was transformed using the Fast Yeast Transformation kit (G-Biosciences, MO, USA). All transformants were selected on a yeast minimal medium without tryptophan ...
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bioRxiv - Genetics 2023Quote: ... coli prepared using Z-Competent cell kit (G-Biosciences, St. Louis, MO) and plated to achieve approximately 104 colonies per plate ...
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bioRxiv - Synthetic Biology 2022Quote: ... and the supernatant was mixed in a 1:1 ratio with HIS-Binding/Wash Buffer (G-Biosciences, USA) and 50 µl HisTag Dynabeads (Thermo Fischer Scientific ...
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bioRxiv - Genetics 2022Quote: ... + 1 μM dexamethasone (G-bioscience, API-04) + 0.5 mM isobutylmethylxanthine (Sigma ...
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bioRxiv - Genomics 2021Quote: ... in 1 ml lysis buffer (G-Biosciences) with 10 µl proteinase K and digested for 1 hour at 60°C with periodic mixing to keep spores suspended in the lysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... supplemented with 1 × Mammalian ProteaseArrest (G-Biosciences), 1 mM phenylmethane sulfonyl fluoride ...
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bioRxiv - Neuroscience 2023Quote: ... and 1 mM PMSF (G-Biosciences, 82021). The cell lysates were clarified by centrifugation at 13,000 g at 4°C for 10 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... Visualization of HRP was accomplished by colorimetric detection (Femto-Chromo HRP kit, G-Biosciences).
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bioRxiv - Genetics 2019Quote: ... 60 and 61 using the OmniPrep™ kit (G-Biosciences, St. Louis, MO, U.S.A.) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Yeast transformation was performed using the Fast™ yeast transformation kit (G-Biosciences, USA) to integrate the linear pAUR101 vector ...
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bioRxiv - Microbiology 2022Quote: ... Particle pellets were resuspended in 1× STE buffer (100 mM NaCl, 10 mM Tris pH 8.0, and 1 mM EDTA) (G-Biosciences, MO). The 293T/17 cells were collected and lysed with RIPA lysis buffer and clarified via centrifugation (1800 x g for 10 min) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 1 mM Phenylmethylsulfonyl fluoride (PMSF) (786-055, G-Biosciences) for 10 min on ice ...
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bioRxiv - Microbiology 2023Quote: ... 1 ml of nickel-chelating resin slurry (G-Biosciences) was added to the cleared lysate ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA was extracted using an OmniPrep Genomic DNA Extraction Kit (G-Biosciences, St. Louis, MO), following the protocol of Espeland et al ...
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bioRxiv - Molecular Biology 2019Quote: ... The samples were pre-treated as per the kit protocol (G-Biosciences, St.Louis, Missouri, USA) and incubated in specific antibody pre-coated wells ...
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bioRxiv - Cancer Biology 2019Quote: ... A non-radioactive colorimetric continuous enzyme kit (#786-430; G-Biosciences, St. Louis, MO, USA) was used to measure the enzyme activity of METTL18 according to the manufacturer’s instructions ...