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Citations for SouthernBiotech :
151 - 200 of 203 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 0,1 % BSA and washed three times with 1× PBS before mounting on microscope slides using Fluoromount-G (Cat# 0100-01, SouthernBiotech,). Hoechst 33342 dye (Cat# B2261 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed three times in 1X PBS for 5 min before being mounted on slides with Fluoromount-G (SouthernBiotech).
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bioRxiv - Developmental Biology 2023Quote: ... Slides were washed three times for five min each and mounted with DAPI Fluoromount-G (0100-20, SouthernBiotech, Birmingham, AL).
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bioRxiv - Developmental Biology 2024Quote: ... The reaction was stopped and washed in PBS three times for 15 min and mounted with fluoro-mount GTM (Southernbiotech).
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bioRxiv - Neuroscience 2020Quote: ... Brain sections were then washed three times in PBST and cover-slipped with DAPI Fluoromount-G mounting medium (SouthernBiotech, #0100-20). Images were captured on a Nikon A1R or Leica SP8 confocal microscope or Olympus VS120 Virtual Microscopy Slide Scanning System.
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bioRxiv - Neuroscience 2020Quote: ... the sections were then rinsed again three to four times in PBS for 5-10 min and mounted on Fisherbrand microscope slides and cover slipped using Fluoromount-G (Southern Biotech) mounting medium.
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bioRxiv - Neuroscience 2020Quote: ... The sections were washed in 1 x PBS three times and mounted and then cover-slipped with Fluoromount-G with DAPI (Southern Biotech).
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bioRxiv - Cell Biology 2021Quote: ... Samples were rinsed three times with PBS between and after antibody incubations and mounted onto slides using Flouromount-G (Southern Biotech).
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bioRxiv - Immunology 2022Quote: ... Plates were washed with water three times before applying 25 μl of secondary antibody (goat anti-human IgG Fc-Southern Biotech, 2048-04 ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed three times and incubated with the goat anti-human-IgG Fc secondary antibody conjugated with alkaline phosphatase (AP, Southern Biotech) at a 1:1000 dilution in blocking buffer for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Stained retinas were washed 3 times with PBS for 10 mins at room temperature and mounted using Fluoromount-G slide mounting medium (Southern Biotech). Flat Mount Retina Slides were proceeded to image with confocal microscopy (Zeiss).
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bioRxiv - Immunology 2020Quote: ... plates were washed 4 times with PBS-T and an anti-monkey IgG conjugated to horseradish peroxidase (Southern Biotech 4700-5) 1 hour at 37°C ...
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bioRxiv - Immunology 2021Quote: ... followed by washing 3 times with PBST and then a 1:20000 dilution of goat anti-rabbit IgG-HRP (Southern Biotech). After washing 3 times with PBST ...
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bioRxiv - Immunology 2020Quote: ... followed by washing 3 times with PBST and then a 1:20000 dilution of goat anti-rabbit IgG-HRP (Southern Biotech). After washing 3 times with PBST ...
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bioRxiv - Immunology 2022Quote: ... The plates were then washed three times with water and 200 μl of goat anti-human IgG HRP secondary antibody (Southern Biotech) diluted to a ratio of 1:2000 in block buffer was added and incubated for 1 hr at room temperature followed by an hour of incubation ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed once with 0.1% BSA / PBS and washed three times with PBS before mounting on microscope slides using Fluoromount-G (SouthernBiotech, 0100-01). For surface labeling ...
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bioRxiv - Cell Biology 2024Quote: ... Labeled cells were washed three times with TBS before being covered with Fluoromount mounting medium (Southern Biotech, Biozol Diagnostica, Eching, Germany). Photos were taken using confocal laser scanning microscopy (TCS SPEII ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were washed with TBS-T 0.1% three times and incubated for one hr at room temperature with goat anti-mouse Ig horseradish peroxidase (HRP)-conjugated antibodies (Southern Biotech, anti-IgM secondary 1:1000 (cat# 1020-05) ...
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bioRxiv - Developmental Biology 2022Quote: ... The slides were washed six times for 20 minutes each in PBS with 0.1% Tween-20 and coverslips were mounted with Fluoromount-G (Southern Biotech 0100-01). The list of antibodies and working dilutions is provided in Table S3 ...
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bioRxiv - Physiology 2020Quote: ... After primary antibody incubation we washed the slides 3 times in PBST and two times in PBS prior to placing the coverslip with DAPI Fluoromount-G as the mounting medium (SouthernBiotech, Birmingham, AL). Fluorescent imaging was performed without antibody amplification in mice administered the AAV8 that encoded for Kir2.1 and tdTomato ...
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bioRxiv - Neuroscience 2021Quote: ... the brain sections were washed three times in PBST and mounted on coverslips with DAPI Fluoromount-G mounting medium (SouthernBiotech, #0100-20). Images were captured on a Zeiss Z2 Apotome or Leica SP8 confocal microscope or Olympus VS120 Virtual Microscopy Slide Scanning System.
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bioRxiv - Bioengineering 2022Quote: ... We washed with PBS three times to remove the unbound fractions and treated the mixtures with anti-human IgG secondary antibody (SouthernBiotech #2016-05). The plate was further incubated at room temperature for 30 min ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... The cells were rinsed three times in 1xPBS and mounted on slides using Fluoromount-G Slide Mounting Medium (Southern Biotech, 0100-01). Slides were images on a Zeiss Axio Observer Z1 microscope coupled to the Perkin Elmer UltraVIEW Vox spinning disk confocal microscopy system ...
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bioRxiv - Neuroscience 2022Quote: ... Retinas were again washed 3 times for 30 minutes each with PBS before a cover slip was attached with Fluoromount-G (SouthernBiotech, Birmingham, Alabama). For RGC counting ...
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bioRxiv - Microbiology 2020Quote: ... washed 3 times in ice-cold PBSM and then bound antibody detected with goat anti-human kappa-A647 (SouthernBiotech, cat# 2060-31) After washing ...
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bioRxiv - Microbiology 2021Quote: ... The plates were washed a second time and incubated for 2 h with Streptavidin-alkaline phosphatase from Southern Biotech (2 µg/mL). The final wash was followed by the addition of Nitro-blue Tetrazolium Chloride/5-bromo-4-chloro 3 ‘indolyphosphate p-toludine salt (NBT/BCIP chromagen ...
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bioRxiv - Neuroscience 2022Quote: ... The stained synaptosomes were washed three more times in TBP before mounting on glass slides with Fluoromount-G (Southern Biotech 0100-01).
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bioRxiv - Immunology 2023Quote: ... plates were washed 3 times and incubated with 50 μl of either 0.25 μg/ml biotinylated anti-mouse IgG1 (Southern Biotech, 1070-08), or biotinylated anti-mouse IgG (H+L ...
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bioRxiv - Cancer Biology 2022Quote: ... the coverslips were washed 3 times with PBS and mounted on microscopy slides using FluoromountG solution containing DAPI (Southern Biotech; 0100-01). Slides were imaged using a Zeiss 880 LSM with Airyscan microscope.
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bioRxiv - Neuroscience 2023Quote: ... The sections were washed four times for 20 minutes in PBS before being mounted on slide glasses with DAPI Fluoromount-G (SouthernBiotech, 0100-20).
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bioRxiv - Neuroscience 2024Quote: ... Retinas were again washed 3 times for 30 minutes each with PBS before a cover slip was attached with Fluoromount-G (SouthernBiotech, Birmingham, Alabama). Images of immunostained wholemounts were acquired with a Keyence epifluorescence microscope (BZ-X800 ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were washed three times with PBST for 15 minutes each and mounted with DAPI Fluoromount G (SouthernBiotech, Cat# 0100-20) for imaging.
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bioRxiv - Neuroscience 2023Quote: ... Cells were then fixed in 4% paraformaldehyde in Hank’s balanced salt solution (HBSS) for 30 min at room temperature and washed 5 times in HBSS before mounting using Fluoromount with DAPI (Southern Biotech, Birmingham, AL).
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bioRxiv - Neuroscience 2023Quote: ... Tissues were washed three more times for 5 minutes each in PBST and then mounted using DAPI Fluoromount-G (0100-20, Southern Biotech, Birmingham, AL). Slides were imaged using a 20x objective on a Zeiss laser scanning confocal microscope (LSM710 ...
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bioRxiv - Microbiology 2024Quote: ... Plates were washed five times with PBS before adding 100 uL of HRP-conjugated goat anti-mouse IgG1 (Southern Biotech, Birmingham, AL, USA) diluted 1:4000 in blocking buffer and were then incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were washed three times in PBS and once in MilliQ water before mounting with FluoromountG solution containing DAPI (Southern Biotech; 0100-01).
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bioRxiv - Bioengineering 2023Quote: ... Cells were then fixed in 3.7% paraformaldehyde in Hank’s balanced salt solution (HBSS) for 30 min at room temperature and washed 5 times in HBSS before mounting using Fluoromount with DAPI (Southern Biotech, Birmingham, AL). Images were acquired using a Zeiss LSM 880 inverted confocal microscope (Carl Zeiss ...
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bioRxiv - Immunology 2020Quote: ... washed 6 times and then incubated with 1: 6000 dilution of horseradish peroxidase (HRP) conjugated anti-mouse IgG secondary antibody (Southern Biotech; Birmingham, AL, USA) for 1 h at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... Then the cells were stained with DAPI solution and washed three times before mounting with Fluoromount-G® Anti-Fade Mounting Medium (Southern Biotech, Birmingham, AL). Images were captured using Zeiss Observer Z1 confocal microscope (Carl Zeiss ...
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bioRxiv - Biophysics 2023Quote: ... we washed the samples 5 times for 5 min with PBS and added one drop of fluoromount-G mounting medium (SouthernBiotech, Cat. No.: 0100-01) to each well prior to applying a cover slip.
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bioRxiv - Cancer Biology 2019Quote: ApoScreen Annexin V-FITC kit from Southern Biotech was used and protocol was followed according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... All cells were maintained at 37 ◻C with 5% CO2 and were mycoplasma free as determined a PCR assay (Southern Biotech).
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bioRxiv - Cell Biology 2024Quote: ... Plates were washed three times with wash buffer and incubated with 100 µL per well of HRP-conjugated α-mouse IgG1 (Southern Biotech, 1070-05, 1:5000 in 1 % BSA in PBS) or HRP-conjugated α-mouse IgM (Southern Biotech ...
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bioRxiv - Immunology 2022Quote: ... Antibody isotyping kit was purchased from Southern Biotech (Cat#5300-05). FITC-conjugated streptavidin was purchased from BD Biosciences (Cat#554060) ...
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bioRxiv - Immunology 2022Quote: ... kit following manufacturer’s protocol for intracellular staining of FITC IgA (Southern Biotech), IgG2b ...
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bioRxiv - Immunology 2020Quote: ... Total serum immunoglobulin levels were detected using SBA Clonotyping System HRP kit (Southern Biotech).
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bioRxiv - Immunology 2022Quote: ... Class-switched serum immunoglobulin levels were detected using SBA Clonotyping System HRP kit (Southern Biotech).
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bioRxiv - Cancer Biology 2023Quote: ... followed by quantification of IgG1 abundance using the SBA Clonotyping System-HRP kit (SouthernBiotech; 5300-05) following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2020Quote: The apoptosis level of PC12 cells was determined by ApoScreen Annexin V kit (SouthernBiotech, Birmingham, AL, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Antibody subtype identification was performed using the SBA ClonotypingTM System/HRP Kit (Southern Biotech, Birmingham, AL, USA). To minimize the background impurities observed during immunoelectron microscopy ...