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Citations for SouthernBiotech :
51 - 100 of 203 citations for hsa mir 185 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... then incubated in 0.1% TBST containing 1:4000 goat anti-rabbit antibody conjugated to HRP for 1 h at RT (SouthernBiotech).
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bioRxiv - Neuroscience 2019Quote: ... washed 3 times in PBS and mounted in DAPI Fluoromount-G® (Southern Biotech). The mouse monoclonal anti-γH2AX antibody (IgG1 ...
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bioRxiv - Pathology 2022Quote: ... washed 3 times in PBS and mounted with DAPI Fluoromount-G (0100-20, SouthernBiotech) and imaged with an inverted SP8 confocal microscope (Leica) ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by detection with a 1:10,000 dilution of goat anti-human IgG-HRP (Southern Biotech 2040-05, Birmingham, AL). Plates were developed for 5 minutes with TMB substrate (VWR 95059-286) ...
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bioRxiv - Immunology 2022Quote: ... IgG antibodies were detected using horseradish peroxidase (HRP)-conjugated goat anti-human IgG detection antibody (Southern Biotech, Birmingham, AL, USA) at a 1:4,000 dilution and colorimetric development was accomplished using 100 μL of 0.1% 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid ...
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bioRxiv - Immunology 2023Quote: ... another microarray was developed with the second detection mix: goat anti-human kappa chain Alexa555 (Southern Biotech, Ref.:2060-32) and goat anti-human lambda chain Alexa647 (Southern Biotech ...
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bioRxiv - Cancer Biology 2023Quote: ... the coverslips were fixed in 4% paraformaldehyde for 20 min at RT and mounted in DAPI Fluoromount-G (SouthernBiotech, USA). Slides were subjected to confocal imaging in a Zeiss LSM 700 microscope (Zeiss ...
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bioRxiv - Developmental Biology 2020Quote: ... washed 3 times in PBST and mounted with Fluoromount-G (Southern Biotech, Birmingham, AL, USA).
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed three times with PBS and mounted using DAPI Fluoromount-G (Southern Biotech). Images were captured on a Zeiss Axioplan 2 Imaging microscope equipped with an AxioCam MRm camera using AxioVision 4.6 software (Zeiss) ...
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bioRxiv - Genetics 2022Quote: ... Coverslips were washed three times with 1x PBS and mounted with Fluoromount-G (Southern Biotech) for imaging acquisition.
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bioRxiv - Neuroscience 2024Quote: ... washed 3 times with PBS and mounted with DAPI Fluoromount-G (Southern Biotech, Birmingham, Alabama). Images of lumbar spinal cord were acquired with an Olympus Confocal Laser Scanning Microscope with FV3000 with 10x and 20x lens ...
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bioRxiv - Immunology 2021Quote: ... and antigen-bound antibodies were detected by using a PE-coupled detection antibody for each subclass and isotype (IgG1, IgG3, IgA1, and IgM; Southern Biotech), and Fcγ-receptors were fluorescently labeled with PE before addition to immune complexes (FcγR2a ...
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bioRxiv - Immunology 2022Quote: ... and antigen-bound antibodies were detected by using a PE-coupled detection antibody for each subclass and isotype (IgG1, IgG2, IgG3, IgG4, IgA1, and IgM; Southern Biotech), and Fcγ-receptors were fluorescently labeled with PE before addition to immune complexes (FcγR2a ...
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bioRxiv - Immunology 2020Quote: An ELISA to detect anti-CCHFV Ig was performed as previously described (Hawman et al., 2019) with an anti-mouse Ig detection antibody (Southern Biotech) to detect all immunoglobulin isotypes or anti-mouse IgG or IgM (Southern Biotech ...
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bioRxiv - Immunology 2021Quote: ... and antigen-bound antibodies were detected by using a PE-coupled detection antibody for each subclass and isotype (IgG1, IgG2, IgG3, IgG4, IgA1, and IgM; Southern Biotech), and Fcγ-receptors were fluorescently labeled with PE before addition to immune complexes (FcγR2A ...
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bioRxiv - Immunology 2023Quote: ... and incubated with pleural lavage fluid overnight at 4°C prior to detection of bound IgM with a goat anti-mouse IgM-HRP (1020-05 Southern Biotech) for 1h at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Equal coating levels of native and denatured TG3 were confirmed by detection of the biotinylated proteins with alkaline phosphatase-conjugated streptavidin (Southern Biotech).
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bioRxiv - Immunology 2023Quote: ... and antigen-bound antibodies were detected by using a PE-coupled detection antibody for each subclass and isotype (IgG1, IgG2, IgG3, IgA1, IgA2 and IgM; Southern Biotech), and Fcγ-receptors were fluorescently labeled with PE before addition to immune complexes (FcγR2A ...
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bioRxiv - Immunology 2023Quote: ... bead bound antigen-specific antibodies were detected by using PE-coupled detection antibody (anti-IgG, IgA1, IgM, IgG1, IgG2, IgG3 and IgG4 from Southern Biotech) (1µg/mL) ...
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bioRxiv - Molecular Biology 2019Quote: ... incubated with secondary antibodies conjugated to Alexa Fluor for one hour at RT and washed with PBS before mounting onto microscope slides using fluoromount-g (Southern Biotech).
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bioRxiv - Systems Biology 2019Quote: ... to stain DNA for 10 min at RT before they were covered with coverslips using Fluoromount-G® (SouthernBiotech; 0100-01). Samples were dried and fluorescence was analysed with a LSM 710 laser scanning microscope (Carl Zeiss ...
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bioRxiv - Bioengineering 2021Quote: ... arrays were incubated for an additional 2 h at RT and 60 rpm with goat anti-mouse IgG conjugated to HRP (Southern Biotech) diluted 1:5000 in blocking solution ...
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bioRxiv - Genetics 2023Quote: ... and verified by PCR to be Mycoplasma free (Southern Biotech).
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bioRxiv - Developmental Biology 2021Quote: ... Sections were washed again 4 times for 20 min before mounting in Fluoromount-G (Southern Biotech).
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bioRxiv - Neuroscience 2020Quote: ... Slides were rinsed three times (5 minutes each) and coverslipped using mounting medium containing DAPI (SouthernBiotech). Images were taken using a Leica TCS SP8 confocal microscope.
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bioRxiv - Bioengineering 2020Quote: ... cells were washed with PBS three times followed by treatment with DAPI Fluoromount-G (Southern Biotech). Cells were imaged with a Leica SP8 3×STED superresolution microscope.
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bioRxiv - Immunology 2021Quote: ... Wells were washed 5 times and secondary goat anti-IgM-HRP (Southern Biotech, cat# 1021-08) at 1:5000 dilution was incubated for 1 hr in the wells ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were washed again four times for 20 min before mounting in Fluoromount-G (Southern Biotech).
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bioRxiv - Immunology 2024Quote: ... plates were washed four times and 25 µl of a 1:500 dilution of the respective detection antibody (goat anti-mouse IgG(H+L)-alkaline phosphatase (AP) conjugate (Southern Biotech/Biozol) or goat anti-mouse IgG1-AP (Southern Biotech/Biozol ...
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bioRxiv - Immunology 2022Quote: ... was added to each well and incubated at RT for 3 minutes and the reaction was terminated with 50 μL of TMB Stop solution (SouthernBiotech, 0412-01). Absorbance at 450 nm (OD450 ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were stained using the ACD supplied DAPI stain for 30 s at RT and the slides were mounted using Fluromount-G (SouthernBiotech, # 0100-01). ACD’s 4-plex positive (#321811 ...
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bioRxiv - Immunology 2023Quote: ... Plates were then incubated for 90min at RT with goat anti-mouse IgG conjugated to horseradish peroxidase (HRP) (Southern Biotech, #1030-05) at 1:5000 dilution in blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... Coverslips were washed thoroughly with 1xPBS at RT 3x 5min each and mounted in mounting medium containing DAPI (DAPI Fluoromount-G™ - Southern Biotech). Antibodies used and concentrations can be found in the Key Resources Table (KRT).
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bioRxiv - Microbiology 2020Quote: ... After washing three times with PBS samples were mounted in Fluoromount G solution containing DAPI (Southern biotech) for DNA staining ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed three times and incubated with HRP-conjugated anti-human IgG Fc (Southern Biotech) for 1 hour at RT ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed three times and incubated with HRP-conjugated anti-human IgG Fc (Southern Biotech) for 1 hour at RT ...
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bioRxiv - Neuroscience 2021Quote: ... cells were washed with PBS (−/−) three times and then cells were mounted using Fluoromount-G® (SouthernBiotech) in the dark for 24 h before imaging.
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bioRxiv - Neuroscience 2019Quote: ... The coverslips were washed 3 times with PBS and mounted with DAPI Fluoromount-G (SouthernBiotech, 0100-20). Between 15-20 pictures of randomly chosen single neurons per experiment were taken with the 63X immersion oil objective and at a magnification of 3X in a Leica TCS SP8 confocal microscope ...
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bioRxiv - Microbiology 2021Quote: ... Plates were then washed 5 times and incubated with goat anti-mouse Ig conjugated to HRP (SouthernBiotech) for 1 hour at room temp ...
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bioRxiv - Neuroscience 2021Quote: ... sections were washed three times in PBS and mounted on microscope slides with DAPI Fluoromount-G (SouthernBiotech). Confocal fluorescence images were acquired on a laser scanning confocal microscope (LSM 780 ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed three times with 1x PBST and mounted with DAPI Fluoromount-G® (SouthernBiotech, AL).
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed three times in 1x PBS and coverslipped in Fluoromount-G (01001-01, Southern Biotech).
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bioRxiv - Immunology 2022Quote: ... Plates were then washed three times and incubated with HRP-conjugated anti-human IgG Fc (Southern Biotech) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were rinsed three times before mounting with anti-fade mounting medium (SouthernBiotech, Cat#: 0100-01).
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bioRxiv - Cell Biology 2022Quote: ... Cells were routinely tested for mycoplasma contamination by PCR (Southern Biotech).
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bioRxiv - Immunology 2021Quote: ... Plates were washed 5 times with PBS/0.05% Tween20 and IgG antibodies were detected using horseradish peroxidase (HRP)-conjugated goat anti-human IgG detection antibody (Southern Biotech, Birmingham, AL, USA) at a 1:4,000 dilution and incubated for 90 min at 37°C ...
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bioRxiv - Immunology 2021Quote: ... For detection of IgG subclasses all serum samples were diluted to 1 total IgG EU and incubated at 37 °C for 1 h prior to detection with Alkaline Phosphatase conjugated anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam) incubated for 1 h at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Staining was performed using anti-PrP antibody (clone ICSM18; D-Gen Ltd) followed by detection with alkaline-phosphatase-linked anti-IgG1 antiserum (Southern Biotech; 1070-04). Spots were visualised with alkaline phosphatase conjugate substrate (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Then samples were washed three times with 0.1% Triton X-100 in PBS for 5 minutes and were incubated 30 minutes at RT with the following secondary antibody diluted 1:200: goat anti-rabbit Alexa Fluor 488 (Southern Biotech 4050-30), goat anti-mouse Alexa Fluor 488 (Southern Biotech 1030-30 ...
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bioRxiv - Microbiology 2021Quote: ... secondary antibody at RT for 2h and stained with 4’,6-diamidino-2-phenylindole (DAPI) before mounting onto slides with Fluoromount-G (Southern Biotech, Birmingham, AL).