Labshake search
Citations for SouthernBiotech :
551 - 600 of 683 citations for T Cell Surface Glycoprotein CD3 Gamma Chain CD3G Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... The plates were washed twice in FACS buffer and stained with 50 μl/well of 1:200 dilution of R-phycoerythrin (PE)-conjugated mouse anti-human IgG Fc antibody (SouthernBiotech #9040-09) and 1:1000 dilution of Zombie-NIR viability dye (BioLegend ...
-
bioRxiv - Cancer Biology 2022Quote: ... Plates were washed with PBS + 0.05% Tween20 and binding was detected with anti-kappa HRP antibody (1:5000, Southern Biotech #2060-05) and developed with the TMB substrate (KPL (Mandel ...
-
bioRxiv - Immunology 2022Quote: ... the cells were stained with 50μl/well of 1:200 diluted R-phycoerythrin (PE)-conjugated mouse anti-human IgG Fc antibody (SouthernBiotech cat.# 9040-09) and 1:1000 dilution of Zombie-NIR viability dye (BioLegend cat.# 423105 ...
-
bioRxiv - Cell Biology 2023Quote: ... The presence of the sLAP proteins was verified by western blotting according to standard protocols and by staining with anti-human HRP antibodies (Southern Biotech, USA). Concentration of sLAP proteins was quantified by dot blotting and using purified human Fc-IgG (SigmaAldrich ...
-
bioRxiv - Immunology 2023Quote: ... MAB273 was detected by adding a 1:5,000 dilution of monkey cross-adsorbed polyclonal goat anti-human IgG HRP antibody (Southern Biotech, 2049-05) and the signal was developed as described above.
-
bioRxiv - Bioengineering 2023Quote: ... followed by 1 hr incubation with horseradish peroxidase-labeled goat anti-mouse secondary antibody (GAM-HRP, SouthernBiotech Cat#1010-05, RRID: AB_2728714). After washing ...
-
bioRxiv - Immunology 2024Quote: ... goat F(ab′)2 anti-human kappa FITC and goat F(ab′)2 anti-human lambda FITC (SouthernBiotech; to detect antibody expression), and PI (Invitrogen ...
-
bioRxiv - Neuroscience 2024Quote: ... Samples were then incubated with AlexaFluor™-conjugated secondary antibodies for two hours at room temperature and briefly rinsed with PBSTr and mounted with DAPI (Southern Biotech).
-
bioRxiv - Immunology 2024Quote: ... plates were washed four times and 25 µl of a 1:500 dilution of the respective detection antibody (goat anti-mouse IgG(H+L)-alkaline phosphatase (AP) conjugate (Southern Biotech/Biozol) or goat anti-mouse IgG1-AP (Southern Biotech/Biozol ...
-
bioRxiv - Cancer Biology 2020Quote: ... All cell lines were mycoplasma free as determined PCR assay (Southern Biotech).
-
bioRxiv - Biophysics 2020Quote: ... Cells were mounted onto glass microscope slides with Fluoromount-G (Southern Biotech) containing DAPI (2.5 μg/mL) ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were then mounted onto slides with Fluoromount with DAPI (Southern Biotech). Micrograph images were obtained using a DeltaVision microscope (Applied Precision ...
-
bioRxiv - Immunology 2020Quote: ... Cells were stimulated with anti-mouse IgM-F(ab)2 (Southern Biotech), ionomycin (4μM ...
-
bioRxiv - Immunology 2022Quote: ... cells were mounted on glass slides with Fluoromount-G (SouthernBiotech; 0100-01) and dried overnight ...
-
bioRxiv - Cell Biology 2020Quote: ... Then samples were washed three times with 0.1% Triton X-100 in PBS for 5 minutes and were incubated 30 minutes at RT with the following secondary antibody diluted 1:200: goat anti-rabbit Alexa Fluor 488 (Southern Biotech 4050-30), goat anti-mouse Alexa Fluor 488 (Southern Biotech 1030-30 ...
-
bioRxiv - Immunology 2021Quote: ... Unbound antibodies were washed away with PBST and bound antibodies detected with 0.5 μg/mL HRP-conjugated goat anti-human constant kappa (2060-05, Southern Biotech, Birmingham, AL, USA) diluted in PBSTB ...
-
bioRxiv - Immunology 2021Quote: ... Plates were again washed and then incubated with 1:4000 dilution of horse radish peroxidase (HRP) conjugated antimouse IgG secondary antibody (SouthernBiotech, cat. 1030-05) and incubated for 1h at RT ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... cells were washed with ice cold PBS buffer and then stained with a PE-labelled anti-mouse IgG secondary antibody (Southern Biotech, Birmingham, AL) at 1 μg/ml concentration for 30 min on ice ...
-
bioRxiv - Biochemistry 2019Quote: ... Binding of glycan-specific IgM and IgG was visualized by a secondary antibody (goat anti-mouse IgM, or goat anti-mouse IgG, Southern Biotech, Birmingham, AL) followed by colorimetric detection ...
-
bioRxiv - Microbiology 2021Quote: ... samples were further statically incubated for 1 h at 4°C with Alexa Fluor 647 conjugated goat-anti-human-kappa F(ab’)2 antibody (Southern Biotech, 1:300) and 6 μM Syto9 (Live/Dead BacLight Bacterial Viability Kit ...
-
bioRxiv - Bioengineering 2021Quote: ... The slides were subsequently incubated at room temperature with secondary antibodies: goat anti-rabbit Ig (1:100 dilution for 15 minutes, 4010-05, Southern Biotech, Birmingham, AL) for CD4 and goat anti-rabbit (1:50 dilution for 30 minutes ...
-
bioRxiv - Bioengineering 2021Quote: ... Antibodies bound to the thymocytes were detected by Cy5-conjugated goat anti-mouse IgG (1:250 dilution, 1030-15, Southern Biotech, Birmingham, AL) and Alexa Fluor 488-conjugated goat anti-mouse IgM (1:250 dilution ...
-
bioRxiv - Immunology 2021Quote: ... For detection of IgG subclasses all serum samples were diluted to 1 total IgG EU and incubated at 37 °C for 1 h prior to detection with Alkaline Phosphatase conjugated anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam) incubated for 1 h at 37 °C ...
-
bioRxiv - Immunology 2020Quote: ... all serum samples were diluted to 1 total IgG ELISA unit and then detected with anti-mouse IgG subclass-specific secondary antibodies (Southern Biotech or Abcam). The results of the IgG subclass ELISA are presented using OD values instead of the ELISA units used for the total IgG ELISA ...
-
bioRxiv - Immunology 2022Quote: ... and stained with a 1:1,000 solution of goat anti-human IgG antibodies conjugated to phycoerythrin (PE) (Southern Biotech, Cat# 2040-09; RRID:AB_2795648) at 4°C for 30 minutes ...
-
bioRxiv - Neuroscience 2022Quote: ... Staining was performed using anti-PrP antibody (clone ICSM18; D-Gen Ltd) followed by detection with alkaline-phosphatase-linked anti-IgG1 antiserum (Southern Biotech; 1070-04). Spots were visualised with alkaline phosphatase conjugate substrate (Bio-Rad ...
-
bioRxiv - Immunology 2023Quote: Biotinylated Goat F(ab)2 anti-human isotype antibodies (anti-human IgM, SouthernBiotech, Cat # 2022-01; anti-human IgA, SouthernBiotech, Cat # 2052-01, and anti-human IgG, SouthernBiotech, Cat # 2042-01) were conjugated on to selected peaks of Streptavidin coated 4um polystyrene capture beads ...
-
bioRxiv - Immunology 2023Quote: ... and incubated with 0.75x serial dilutions of purified polyclonal human antibodies (IgG, SouthernBiotech, Cat # 0150-01; IgM, SouthernBiotech, Cat # 0158L-01; IgA, SouthernBiotech, Cat # 0155K-01) at known concentrations ...
-
bioRxiv - Immunology 2023Quote: ... The plates were washed and incubated with either AP-conjugated goat anti-mouse IgG or IgM antibody (SouthernBiotech; cat # 1030-04, 1020-04) (Supplementary Table 8) ...
-
bioRxiv - Immunology 2023Quote: ... The SOSIP-specific IgG response in the serially diluted RM plasma was measured using an anti-monkey IgG horseradish peroxidase (HRP)-conjugated secondary antibody at 1:4000 dilution (Southern Biotech, Birmingham, AL) and concentration of the CH505-SOSIP-specific IgG response was estimated using a standard curve of CH505 T/F SOSIP IgG detection by Rh HIV-specific monoclonal antibody ...
-
bioRxiv - Immunology 2023Quote: ... and the development of ADA in plasma samples were detected using a goat anti-human lambda IgG HRP-conjugated secondary antibody at 1:4000 dilution (Southern Biotech, Birmingham, AL). The plasma ADA levels were reported as endpoint titer ...
-
bioRxiv - Neuroscience 2024Quote: ... They were then washed with PBS and incubated for 1 h at room temperature with the appropriate secondary antibody: goat anti-Rabbit IgG-HRP (Southern Biotech, #4030-05) or goat anti-Mouse IgG-HRP (Southern Biotech ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were thoroughly washed in PBS prior to mounting in Fluoromount-G (SouthernBiotech) on glass slides.
-
bioRxiv - Developmental Biology 2021Quote: ... and cells were mounted in Fluoromount G (Southern Biotech cat. no. 0100-01). Images were acquired using a Zeiss LSM780 point scanning confocal microscope with a 20X objective.
-
bioRxiv - Immunology 2022Quote: ... Cells were mounted using water-based mowiol mounting media (Southern Biotech, Birmingham, AL) to preserve fluorescence ...
-
bioRxiv - Cancer Biology 2019Quote: ... Cells were then washed and mounted with DAPI Fluoromount-G (SouthernBiotech, Birmingham, AL). Images were acquired with a confocal laser-scanning inverted microscope (Leica Confocal ...
-
bioRxiv - Immunology 2022Quote: ... Cells were mounted using water-based mowiol mounting media (Southern Biotech, Birmingham, AL) to preserve fluorescence ...
-
bioRxiv - Immunology 2020Quote: ... cells were incubated with PE-conjugated mouse anti-human IgG Fc (Southern Biotech) diluted 1:200 at 4°C for 30 minutes ...
-
bioRxiv - Immunology 2020Quote: ... cells were stained with FITC-conjugated goat-anti-Rhesus polyclonal antisera (Southern Biotech) to detect binding of the plasma Ab (pAb) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were mounted to glass slides using Fluoromeount G DAPI (SouthernBiotech; 0100-20) and imaged on a Zeiss 800 CLSM with Airyscan at the UNMC Advanced Microscopy Core Facility.
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were washed and embedded on glass slides with DAPI Fluoromount-G (SouthernBiotech). Mean nuclear signal is mean gray value calculated with image analysis using FIJI.
-
bioRxiv - Molecular Biology 2023Quote: ... coverslips with cells were then mounted in Fluoromount-G® mounting medium (SouthernBiotech) on microscope slides ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were washed three times in PBS and mounted in Fluoromount (Southern Biotech).
-
bioRxiv - Immunology 2023Quote: ... cells were resuspended in 1:200 of anti-monkey IgG-PE (Southern Biotech) for 25 minutes at RT ...
-
bioRxiv - Microbiology 2023Quote: ... All cell cultures were verified by PCR to be Mycoplasma-free (Southern Biotech).
-
bioRxiv - Bioengineering 2023Quote: ... or without cell type specificity (rabbit anti-human IgG, 6145-01, Southern Biotech) was dissolved in PBS ...
-
bioRxiv - Neuroscience 2023Quote: ... The stained sections or cells were mounted onto slides with Fluoromount-G (SouthernBiotech) or ProlongTM Glass Antifade Mountant (Invitrogen ...
-
bioRxiv - Biochemistry 2020Quote: ... the culture media were tested for their ACE2-Fc contents using a custom ELISA (anti-ACE2 antibody [Abcam, Cambridge, MA] for capturing and anti-human IgG-Fc-HRP [SouthernBiotech, Birmingham, AL] for detection). Clones with the highest expression of recombinant ACE2-Fc variants were selected ...
-
bioRxiv - Immunology 2022Quote: ... Plates were washed with water three times before applying 25 μl of secondary antibody (goat anti-human IgG Fc-Southern Biotech, 2048-04; goat anti-mouse IgG Fc-Southern Biotech, 1033-04) at a dilution of 1:4,000 in blocking buffer ...
-
bioRxiv - Immunology 2020Quote: ... washed 6 times and then incubated with 1: 6000 dilution of horseradish peroxidase (HRP) conjugated anti-mouse IgG secondary antibody (Southern Biotech; Birmingham, AL, USA) for 1 h at RT ...