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Citations for SouthernBiotech :
51 - 100 of 103 citations for Streptavidin FITC Conjugate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and for sMAC with 1:5,000 HRP-conjugated streptavidin (Southern Biotech). Finally ...
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bioRxiv - Immunology 2020Quote: ... plates were washed and incubated with streptavidin-alkaline phosphatase (Southern Biotech) for 2 hours at room temperature ...
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bioRxiv - Immunology 2023Quote: ... followed by an incubation of 1:5000 streptavidin-HRP (Southern Biotech). To measure deposition of C3b on the IgM coated plates ...
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bioRxiv - Immunology 2022Quote: ... plus HRP-conjugated streptavidin (1:7000, Southern Biotech, Cat# 7100-05) were used for measuring mouse IgA antibody ...
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bioRxiv - Immunology 2021Quote: ... followed by goat F(ab’)2 anti-mouse IgG1-FITC (Southern Biotech). The percentage of living HUCCT1 cells was estimated by flow cytometry ...
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bioRxiv - Immunology 2022Quote: ... kit following manufacturer’s protocol for intracellular staining of FITC IgA (Southern Biotech), IgG2b ...
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bioRxiv - Microbiology 2022Quote: ... or 2 μg/ml goat anti-hu-IgM-FITC (2020-02, SouthernBiotech) for 30 minutes at 4 °C while shaking ...
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bioRxiv - Immunology 2022Quote: ... followed by incubation with streptavidin-HRP (Sigma-Aldrich, Invitrogen and Southern Biotech), and the reaction was detected using TMB substrate (BD biosciences) ...
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bioRxiv - Immunology 2021Quote: ... Biotinylated antibodies were subsequently probed with streptavidin-HRP (Southern Biotech, 7100-05). Detection antibody was removed ...
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bioRxiv - Immunology 2020Quote: ... cells were stained with FITC-conjugated goat-anti-Rhesus polyclonal antisera (Southern Biotech) to detect binding of the plasma Ab (pAb) ...
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bioRxiv - Immunology 2021Quote: ... Binding was visualized via incubation with anti-kappa-FITC (H139-52.1) (Southern Biotech). Imaging was performed with a Leica DM4000 fluorescent scope.
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bioRxiv - Immunology 2022Quote: ... Plates were washed and incubated with 100 μl HRP-conjugated streptavidin (Southern Biotech) at a dilution of 1:1000 for 1 h at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Goat anti-mouse IgG antibody tagged with streptavidin-HRP (Southern Biotech, 7105-05) was diluted 1:400 in blocking buffer and incubated for 1 hr at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Antibody binding to the peptides was determined using a goat anti-human IgG HRP conjugate (ITK Southern Biotech) for 1 h at RT and tetramethylbenzidine substrate (BioFX) ...
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bioRxiv - Immunology 2019Quote: ... incubated with serum diluted 1:100 and stained with anti-IgG-FITC (Southern Biotech) (10 μg/ml) ...
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bioRxiv - Immunology 2019Quote: ... Staining was detected using goat anti-mouse IgG FITC at 1:500 (Southern Biotech) ANAs were scored on intensity of nuclear and cytoplasmic staining on a scale of 0–3 and for the presence or absence of mitotic chromatin using wide-field fluorescence microscopy (Olympus IX83 ...
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bioRxiv - Immunology 2021Quote: ... The following antibodies were used (monoclonal unless indicated): IgD FITC (goat polyclonal, Southern Biotech), IgM PerCP-Cy5.5 (clone G20-127 ...
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bioRxiv - Immunology 2021Quote: ... The following antibodies were used (monoclonal unless indicated): IgD FITC (goat polyclonal, Southern Biotech), IgM PerCP-Cy5.5 (clone G20-127 ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed as previously and 100 µL/well of streptavidin-AP (Southern Biotech) diluted 1:1000 was added ...
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bioRxiv - Immunology 2021Quote: ... then incubated for 2 h at room temperature with Streptavidin-alkaline phosphatase from Southern Biotech at 2 μg/mL ...
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bioRxiv - Immunology 2021Quote: ... Excess biotinylated anti-IgA antibody was removed by washing and streptavidin-conjugated HRP (Southern Biotech) was added ...
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bioRxiv - Immunology 2022Quote: ... Plates were then washed as previously and 50 µL/well of streptavidin AP (Southern Biotech) diluted 1:1000 in PBS was added and plates incubated at RT for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... and bound biotinylated IgG was detected using horseradish peroxidase-conjugated streptavidin (Southern Biotech, Birmingham, AL) (1 hour ...
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bioRxiv - Immunology 2023Quote: ... Binding of the biotinylated mAb was subsequently detected using alkaline phosphatase-conjugated streptavidin (Southern Biotech). The ability of IgG1 mAbs to compete with serum IgA for TG3 binding was assessed in a similar way ...
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bioRxiv - Immunology 2022Quote: ... the hACE2 receptor bound to the S protein was then detected using a mouse anti-His-Tag horseradish peroxidase conjugate (Southern Biotech) and a colorimetric signal generated by the addition of 3,3′,5,5′-TMB substrate ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed and incubated for 5 min in ice with streptavidin (60 μg/ml, SouthernBiotech) for cross-linking and incubated at 37°C ...
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bioRxiv - Immunology 2019Quote: ... the membrane was incubated with a 1: 2500 dilution of streptavidin-HRP (Southern Biotech; 7100-05) in 5% BSA/1x TBST for 1 hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... the plates were washed and developed with streptavidin-HRP antibody (1:1000; SouthernBiotech, Birmingham, AL, USA) and 3,3′,5,5′-tetramethylbenzidine (TMB ...
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bioRxiv - Immunology 2022Quote: ... Sections were washed and stained with anti-IgA FITC (clone 11-44-2, Southern Biotech, 1:100) in blocking buffer in the dark for 30min at RT ...
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bioRxiv - Immunology 2020Quote: ... except constructs were bound directly to the plate and detected with goat anti-mouse IgG HRP conjugate (Southern Biotech, Birmingham, AL, USA).
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bioRxiv - Immunology 2024Quote: ... plates were washed four times and 25 µl of a 1:500 dilution of the respective detection antibody (goat anti-mouse IgG(H+L)-alkaline phosphatase (AP) conjugate (Southern Biotech/Biozol) or goat anti-mouse IgG1-AP (Southern Biotech/Biozol ...
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bioRxiv - Immunology 2022Quote: ... Cells were further incubated with biotinylated goat anti-rabbit IgG (H+L) and HRP-streptavidin (Southern Biotech). The reactions were developed with o-Phenylenediamine di-hydrochloridec (OPD ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed with PBS and incubated for 1 h with a secondary goat anti-mouse FITC (SouthernBiotech) at a 20 μg/mL concentration and phalloidin (7 units ...
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bioRxiv - Immunology 2020Quote: ... Plates were then incubated with a 1:1000 dilution of mouse serum raised against ZE3-6H followed by goat anti-mouse IgG HRP conjugate (Southern Biotech, Birmingham, AL, USA) for 1 h at 37°C ...
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bioRxiv - Immunology 2021Quote: ... and permeabilized prior to staining with a FITC-conjugated goat anti-rhesus IgG (H+L) polyclonal antibody (Southern Biotech). Data represent the frequency of cells positive for IgG-binding to Env for post-vaccination samples compared to the pre-vaccination sample ...
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bioRxiv - Immunology 2022Quote: ... cryopreserved PBMC were thawed and stained with the following antibodies (monoclonal unless indicated): IgD FITC (goat polyclonal, Southern Biotech), IgM PerCP-Cy5.5 (clone G20-127 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then surface stained with mouse anti-chicken Bu-1-FITC (Clone AV20; Southern Biotech, Birmingham, AL, USA) and mouse anti-chicken monocyte/macrophage-PE (Clone KUL01 ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were stained again for 30 min with 0.5 µg of FITC-conjugated anti-mouse IgA antibody (Southern Biotech) in 100 µl of staining buffer to refresh and harmonize the staining between batches ...
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bioRxiv - Immunology 2023Quote: ... cryopreserved PBMC were thawed and stained with the following antibodies (monoclonal unless indicated): IgD FITC (goat polyclonal, Southern Biotech), IgM PerCP-Cy5.5 (clone G20-127 ...
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bioRxiv - Immunology 2024Quote: ... with serum diluted 1:10 followed by an incubation with goat anti-mouse IgG-FITC (1:100; Southern Biotech). Images were acquired on an inverted fluorescence microscope (Olympus) ...
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bioRxiv - Microbiology 2024Quote: ... Antibodies used for flow cytometry analysis performed in this study were: FITC-conjugated anti-human IgM (Southern Biotech #2020-02), goat anti-human IgG-UNLB (Southern Biotech #2040-01) ...
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bioRxiv - Immunology 2020Quote: ... The following antibody cocktails were used for B cell (FITC anti-mouse CD43, Biolegend, 553270; PE Goat anti-mouse IgM, Southern Biotech, 1020-09 ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were tryprinized and resuspended in binding buffer at 72 hours post-transfection and incubated with Annexin V-FITC (Southern Biotech) and propidium iodide (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... buffer (PBS with 0.1% bovine serum albumin and 0.1% sodium azide) containing the PE-labeled tetrameric pBF2*1501 complex and fluorescein isothiocyanate (FITC)-labeled anti-CD8 monoclonal antibody (SouthernBiotech, USA) for 30 min at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... 1×109 bacteria were washed with 1 ml ice-cold PBS and stained for 30 min at 4°C with 4 µg of FITC-conjugated anti-mouse IgA antibody (Southern Biotech) in 100 µl of staining buffer ...
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bioRxiv - Systems Biology 2022Quote: ... for capture and biotinylated anti-human insulin antibody (Eli Lilly and Company) with horseradish peroxidase conjugated streptavidin (7100-05, Southern Biotech) for detection ...
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bioRxiv - Immunology 2023Quote: ... Equal coating levels of native and denatured TG3 were confirmed by detection of the biotinylated proteins with alkaline phosphatase-conjugated streptavidin (Southern Biotech).
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bioRxiv - Immunology 2023Quote: ... Sections were washed in PBS before incubation with secondary antibodies or labelled streptavidin (Supplementary Table 4) for 1hr at room temperature followed by mounting with DAPI containing fluoromount (Southern Biotech). Small to medium sized airways in lung sections were captured by an investigator blind to sample identity with an EVOS FL imaging system (Thermo) ...
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bioRxiv - Immunology 2022Quote: ... NP-specific IgM and IgG3 antibodies were detected using biotinylated anti-mouse IgM- or IgG3-specific immunoglobulins followed by streptavidin-HRP (Southern Biotech) and developed with SIGMAFAST OPD tablets (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... Bound IL-21 was subsequently detected with mouse anti-human IL-21-biotin (clone 2B2-G20, eBioscience) followed by alkaline phosphatase-conjugated streptavidin (Southern Biotech). The signals were compared to a standard curve generated with recombinant human IL-21 (R&D Systems).