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Citations for SouthernBiotech :
101 - 150 of 158 citations for Small Airway Epithelial Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... This cell line was routinely tested for mycoplasma contamination using a Mycoplasma Detection Kit (SouthernBiotech, Cat# 13100-01).
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bioRxiv - Cell Biology 2021Quote: ... and coverslips were mounted on top of the cells using Fluoromount-G mounting solution containing DAPI (Southern Biotech). Single slice images were taken on a Zeiss 880 microscope using the confocal settings.
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bioRxiv - Microbiology 2020Quote: ... Cells were washed a further three times before mounting with Fluoromount G with DAPI (Cambridge Bioscience, Southern Biotech). Imaging was performed with an Axioscope 2 fluorescence microscope (Zeiss ...
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bioRxiv - Immunology 2020Quote: ... Cells were then surface stained with S/RBD probes and the following antibodies: IgD AF488 (polyclonal; Southern Biotech), IgM BUV395 (G20-127) ...
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bioRxiv - Neuroscience 2021Quote: ... cell nuclei were stained using DAPI during the first round of HCR-FISH staining (DAPI Fluoromount-G; SouthernBiotech).
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bioRxiv - Immunology 2023Quote: ... Cells were either left unstimulated or 20 µg/mL of F(ab’)2 anti-human IgM (Southern Biotech) was added directly to the well ...
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bioRxiv - Immunology 2023Quote: ... cells were either left unstimulated or 20 µg/mL of F(ab’)2 anti-human IgM (Southern Biotech) was added directly to the well ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were routinely screened and confirmed negative for mycoplasma contamination at least every three months (SouthernBiotech #13100-01).
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bioRxiv - Microbiology 2023Quote: ... Cells were washed with PBS and incubated for 1 h with a secondary goat anti-mouse FITC (SouthernBiotech) at a 20 μg/mL concentration and phalloidin (7 units ...
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bioRxiv - Immunology 2023Quote: ... Cells were either left unstimulated or 20 µg/mL of F(ab’)2 anti-human IgM (Southern Biotech) was added directly to the well ...
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bioRxiv - Immunology 2023Quote: ... cover slips with stained cells were mounted with DAPI Fluoromount-G (Southern Biotech, AL, USA, Cat. 0100-20) on microscope slides and analyzed by an AxioObserver Z.1 Microscope with 40x or 63x objectives (Zeiss ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed 3 times with PBST and mounted on slides with Fluoromount-G (SouthernBiotech #0100-01) or ProLong™ Glass Antifade Mountant (Invitrogen P36984) ...
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bioRxiv - Immunology 2024Quote: ... Human B cell stimulation used 10ng/mL Goat F(ab’)2 Anti-human IgM (Southern Biotech #2022-01), 2ug/mL of recombinant human sCD40 (invitrogen PHP0025) ...
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bioRxiv - Cell Biology 2019Quote: ... The fixed cells were washed with PBS twice and resuspended in a mounting medium (Dapi Fluoromount-G; Southern Biotech). The samples were put on microscope slides ...
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bioRxiv - Biochemistry 2021Quote: ... Cells lines were authenticated and tested for mycoplasma contamination before use via the Mycoplasma Detection Kit (SouthernBiotech; 13100-01). Cells were maintained in a humidified atmosphere of 5% CO2 and 37 °C and were passaged every 2-3 days into 10 cm polystyrene coated plates (Eppendorf ...
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bioRxiv - Cell Biology 2021Quote: ... Afterward cells were fixed with 4% FA and mounted on glass slides using Fluoromount-G mounting medium (Southern Biotech). Images of the cells were acquired using confocal microscopy and analyzed with ZEN software (Carl Zeiss).
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bioRxiv - Cell Biology 2021Quote: ... Afterward cells were fixed with 4% FA and mounted on glass slides using Fluoromount-G mounting medium (Southern Biotech). Images of the cells were acquired using confocal microscopy and analyzed with ZEN software (Carl Zeiss).
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bioRxiv - Neuroscience 2020Quote: ... the cover slips with cultured cortical cells or slides with brain tissue were either mounted with Fluoromount-G (SouthernBiotech) or subjected to a subsequent immunocytochemistry ...
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bioRxiv - Physiology 2020Quote: ... Cells were also regularly checked for mycoplasma contamination using a PCR based mycoplasma detection kit from SouthernBiotech (Birmingham, AL) and were confirmed to be negative ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then surface stained with mouse anti-chicken Bu-1-FITC (Clone AV20; Southern Biotech, Birmingham, AL, USA) and mouse anti-chicken monocyte/macrophage-PE (Clone KUL01 ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed with 1% BSA-PBS and labeled with APC-conjugated goat anti-rat immunoglobulin antibody (Southern Biotech) at 25°C for 20 min ...
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bioRxiv - Immunology 2021Quote: ... The cells were washed with FACS buffer and incubated with secondary goat anti-human IgG PE antibody (Southern Biotech) for 1 hour at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... The cell nuclei were labeled with a DAPI stain present in the mounting media (DAPI Fluoromount-G, Southern Biotech). Images were acquired using an upright microscope (Echo Revolve ...
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bioRxiv - Immunology 2022Quote: ... cells secreting anti-NP antibody (ASC) were visualized with HRP-conjugated anti-mouse IgM or IgG3 antibodies (Southern Biotech) followed by AEC staining Kit (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed five times in PBS and mounted on glass slides using Fluoromount G (Southern Biotech; 0100-01). Prepared specimens were imaged on a Leica DMi8 (LAS X software ...
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bioRxiv - Cancer Biology 2020Quote: ... Jurkat/NF-κB-GFP-hCD40 reporter cells were incubated with different concentrations of CD40 agonist antibodies with or without goat anti-human Fc antibody (SouthernBiotech) for 24 hours ...
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bioRxiv - Immunology 2020Quote: ... 4 single cell clones of DT40 WT or Fam72a−/− were stained with anti-IgM-PE (1:40, clone M-1, SouthernBiotech) and 12 populations of 5×104 IgM− cells were sorted for each clone ...
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bioRxiv - Immunology 2022Quote: ... fixed blood cells were washed in bicarbonate buffer and stained using antibodies against CD71 (Southern Biotech, #1720-02, 1:200) and Ter119 (eBioscience ...
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bioRxiv - Microbiology 2020Quote: ... Cell lysates and supernatants were subjected to immunoblot analysis for antibodies against cellular LAMP-1 (1:2000, SouthernBiotech, 9835-01), VSV-G (1:2000 ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were then washed in 1xPBS 3x for 5mins and mounted in Fluoromount-G (Southern Biotech cat no 0100-01). Images were then acquired using a NikonA1R confocal microscope using a 60X ...
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bioRxiv - Immunology 2021Quote: ... the pellet was resuspended in a 50 μL mixture of PE-anti-human CD11b (Southern Biotech, 10 μL/million cells), Alexa Fluor 647-anti-human CD35 (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with FACs buffer (1% BSA, 1% Na+azide) and secondary antibody IgG-PE (Southern Biotech, Alabama, USA) was added in a 1:200 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed 3x with DPBS and coverslips mounted on 1 mm glass slides using Fluoromount-G (SouthernBiotech, #0100-01). Cells were imaged using a Zeiss Axio Observer 7 fitted with a 40x objective using DAPI and Alexa Fluor 488 filters ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were mounted on glass slides in a 1:1 ratio of YC complete:DAPI Fluoromount-G (SouthernBiotech, Cat #0100-20). Yeast were imaged with a Zeiss 880 LSM with fast Airyscan.
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bioRxiv - Molecular Biology 2023Quote: ... cells were fixed with 4% paraformaldehyde for 30 min at room temperature and stained with DAPI Fluoromount-G (Southern Biotech) to observe intracellular localization using an Olympus BX63 fluorescence microscope ...
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bioRxiv - Cell Biology 2023Quote: ... Cells cultured on glass coverslips were then lifted and mounted on microscope slides using Fluoromount-G Mounting Medium (Southern Biotech) and stored at 4C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... tissue slices and cell culture slices were covered by a glass slice with the mounting medium (SouthernBiotech, 0100-01, USA). Zeiss LSM800 (Zeiss ...
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bioRxiv - Immunology 2021Quote: ... and double-distilled water to remove cells before being incubated with alkaline phosphatase-conjugated anti-mouse IgM or anti-mouse IgG1 (Southern Biotech) for 2 h at room temperature ...
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bioRxiv - Immunology 2020Quote: ... The following antibody cocktails were used for B cell (FITC anti-mouse CD43, Biolegend, 553270; PE Goat anti-mouse IgM, Southern Biotech, 1020-09 ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were tryprinized and resuspended in binding buffer at 72 hours post-transfection and incubated with Annexin V-FITC (Southern Biotech) and propidium iodide (Sigma Aldrich ...
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bioRxiv - Immunology 2019Quote: ... Cells were washed with PBS–0.1% Tween 20 and incubated with mouse anti-human IgG Fc-HRP (Southern Biotech; 9040-05) for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... The cells were then stained with 50 μL of the secondary mix (goat anti-human IgG-AF488 (SouthernBiotech, cat. #2040-30) and goat anti-mouse Alexa Fluor 488 IgG (H + L ...
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bioRxiv - Immunology 2022Quote: ... Cells were depleted of B cells and partially depleted of AMs and cells that had lost membrane integrity by first incubating with anti-IgD Biotin (Southern Biotech) and anti-Siglec-F Biotin ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lines were authenticated every 6 – 12 months through STR profiling at MD Anderson’s Cytogenetics and Cell Authentication Core and were routinely checked for mycoplasma (SouthernBiotech #13100-01). Cen3tel cells were authenticated as having distinct STR profiles from all other cell lines and being similar to each other across PDs ...
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bioRxiv - Cell Biology 2024Quote: ... Labeled cells were washed three times with TBS before being covered with Fluoromount mounting medium (Southern Biotech, Biozol Diagnostica, Eching, Germany). Photos were taken using confocal laser scanning microscopy (TCS SPEII ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips with stained cells were washed 2 x 5 min in PBS and then mounted with Fluoromount-G (Southern Biotech 0100-01) for image acquisition ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... The cells were rinsed three times in 1xPBS and mounted on slides using Fluoromount-G Slide Mounting Medium (Southern Biotech, 0100-01). Slides were images on a Zeiss Axio Observer Z1 microscope coupled to the Perkin Elmer UltraVIEW Vox spinning disk confocal microscopy system ...
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bioRxiv - Immunology 2019Quote: ... 4 × 106 primary B cells in 0.1 ml of imaging medium were stimulated with 20 µg/ml soluble goat anti-Igκ antibodies (Southern Biotech, #1050-01). Cells were lysed in RIPA buffer (30 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2020Quote: ... All cells were maintained at 37 ◻C with 5% CO2 and were mycoplasma free as determined a PCR assay (Southern Biotech).
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bioRxiv - Microbiology 2023Quote: ... cells were attached to a poly-L-lysine treated slide before 5 μl of Fluoromount G with DAPI (Cambridge Bioscience, Southern Biotech) was added and coverslip applied ...