Labshake search
Citations for SouthernBiotech :
51 - 100 of 217 citations for SARS CoV 2 Spike Glycoprotein S2 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... cells were incubated with goat anti-human IgG Fc-HRP (Southern Biotech, 2014-05) diluted at 1:1000 in Perm Wash for 1.5 h at room temperature ...
-
bioRxiv - Immunology 2022Quote: ... Secondary detection antibody solutions of Mouse Anti-Human IgG Fc-HRP (SouthernBiotech, Birmingham, AL) diluted 1:10,000 in 1% milk PBST and Mouse Anti-Human IgA1-HRP/Mouse Anti-Human IgA2-HRP (SouthernBiotech ...
-
bioRxiv - Immunology 2023Quote: ... and 100 µL of anti-human IgG-Fc HRP (clone JDC-10, Southern Biotech), diluted 1:2000 in blocking buffer ...
-
bioRxiv - Immunology 2024Quote: ... and Goat anti-Mouse IgG Fc Human ads-PE (Southern Biotech, catalog:1030-09) were added to the beads (6 μg/ml in block buffer ...
-
bioRxiv - Microbiology 2020Quote: ... Antibody binding to the spike proteins was determined using a 1:2000 diluted HRP conjugated goat anti-human IgG (ITK Southern Biotech) for 1 h at RT and tetramethylbenzidine substrate (BioFX) ...
-
bioRxiv - Immunology 2019Quote: A20 D1.3 cells were stained on ice for 10 min with anti-IgM-biotin (Southern Biotech) or HEL-biotin and washed with PBS ...
-
bioRxiv - Biochemistry 2023Quote: ... followed by the addition of 1/10,000 goat anti-mouse IgA-biotin (Southern Biotech, #1040-08) or 1/10,000 goat anti-IgG-biotin (Southern Biotech #1030-08 ...
-
bioRxiv - Immunology 2022Quote: ... bound IgG was detected using an HRP-conjugated goat anti-human Fc antibody (Southern Biotech) at a dilution of 1:10,000 in blocking buffer for 1 hour at RT ...
-
bioRxiv - Immunology 2021Quote: ... Plates were then washed and goat anti-mouse IgG Fc-HRP (Southern Biotech, 1013-05) was added to the plate at 1:2000 in PBS ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were washed then coincubated with PE-conjugated mouse anti-human IgG Fc (Southern Biotech) diluted to 1:200 for 30 minutes at 4°C ...
-
bioRxiv - Microbiology 2022Quote: ... Slides were exposed to biotin labelled goat Fab anti-human IgG secondary antibody (Southern Biotech, Birmingham, AL) at a 1:2500 dilution for 1 h at room temperature ...
-
bioRxiv - Immunology 2022Quote: ... and biotin-labeled goat anti-mouse IgA (Cat# 1040-08) were obtained from Southern Biotech (Birmingham, AL). HRP-conjugated anti-mouse IgG Fab (Cat# A9917 ...
-
bioRxiv - Immunology 2021Quote: ... A medium density capture chip was created with anti-Human IgG Fc (SouthernBiotech, Birmingham, AL, USA) with a surface density of 925-1200 RU ...
-
bioRxiv - Microbiology 2019Quote: ... and 100 µl/well of goat anti-human IgG Fc-BIOT (Cat# 2014-08; Southern Biotech) secondary antibody diluted 1:10,000 in binding buffer was added ...
-
bioRxiv - Immunology 2021Quote: ... HRP-conjugated mouse anti-histidine-tag IgG (1:4000) (catalog number 4603-05, Southern Biotech, Birmingham, AL, USA) was added for 1 hour followed by addition of TMB substrate ...
-
bioRxiv - Immunology 2022Quote: ... the plates were incubated with serially diluted serum samples and then with biotin-conjugated detection antibodies (Southern Biotech) followed by streptavidin-conjugated HRP (Jackson Immunoresearch) ...
-
bioRxiv - Immunology 2021Quote: ... Plates were then washed three times and incubated with HRP-conjugated anti-human IgG Fc (Southern Biotech) for 1 hour at RT ...
-
bioRxiv - Immunology 2020Quote: ... bound antibody was detected using HRP-conjugated goat anti-human Fc multiple species cross-absorbed (Southern Biotech) for 1 h at 4°C ...
-
bioRxiv - Immunology 2021Quote: ... Plates were then washed three times and incubated with HRP-conjugated anti-human IgG Fc (Southern Biotech) for 1 hour at RT ...
-
bioRxiv - Immunology 2019Quote: ... Blots were washed and bands were detected with anti-mouse IgG Fc-HRP (Southern Biotech; 1030-05) at 1:10,000 for 1 hour at room temperature ...
-
bioRxiv - Immunology 2022Quote: ... Plates were then washed three times and incubated with HRP-conjugated anti-human IgG Fc (Southern Biotech) for 1 hour at room temperature ...
-
bioRxiv - Microbiology 2023Quote: ... washed then incubated for 1 hour with phycoerythrin labeled anti-human IgG Fc (Southern Biotech, Birmingham AL) detection antibody ...
-
bioRxiv - Microbiology 2022Quote: ... or 1:5,000 mouse anti-His-Tag (primary) then 1:5000 goat anti-mouse IgG(H+L)-HRP (secondary) (SouthernBiotech). Blots were developed with ECL Western Blotting Substrate (Pierce ...
-
bioRxiv - Neuroscience 2021Quote: ... samples were labelled with biotin-coupled antibodies (all antibodies were purchased from Southern Biotech: goat anti mouse (1012-08), goat anti rat (3052-08) ...
-
bioRxiv - Immunology 2019Quote: ... The plates were then washed and incubated with peroxidase-conjugated goat anti-mouse IgM Fc-specific (Southern Biotech). In differentiation assays ...
-
bioRxiv - Biochemistry 2021Quote: ... and the secondary antibody solution was a 1:1200 dilution of goat-anti-human IgG Fc-HRP (SouthernBiotech). ELISA curves were fit to a 4-parameter logistic curve (Fig ...
-
bioRxiv - Immunology 2024Quote: ... 50 μl of Goat anti-Human IgG Fc Multi-species SP ads-PE (Southern Biotech, catalog:2014-09) and Goat anti-Mouse IgG Fc Human ads-PE (Southern Biotech ...
-
bioRxiv - Microbiology 2020Quote: ... membranes were washed with TBST and imaged (for Strep-Tag II HRP) or incubated with goat anti-rabbit-HRP (1:5,000; Southern Biotech).
-
bioRxiv - Immunology 2022Quote: ... Plates were washed 3X with PBS and 3X with PBS-T and then anti-mouse IgG/IgA-biotin (Southern Biotech) diluted 1:1000 in PBS-T was added and incubated overnight at 4 °C ...
-
bioRxiv - Immunology 2022Quote: ... A volume of 25 μL of a 1:1,000 dilution of Mouse Anti-Biotin-AP (Southern Biotech, cat. #6404-04) in blocking buffer was added to each of the wells of the HN plates and incubated for 1 hr at RT ...
-
bioRxiv - Biochemistry 2024Quote: ... PVDF membranes containing blotted proteins were washed in excess PBS-Tween 20 for 1h at room temperature before application of a 1:2000 dilution of primary mouse Anti-Human IgA1 antibodies conjugated to biotin (SouthernBiotech). Blots were washed 10 min 3 x in PBS followed by treatment with a 1:2000 dilution of ExtrAvidin®−Peroxidase conjugate (Sigma ...
-
bioRxiv - Immunology 2023Quote: ... PE conjugated goat anti-human IgG Fc secondary Ab was added at 6μg/ml (Southern Biotech, catalog: 2014-09) and incubated for 1 hour at 37°C with shaking at 700 rpm ...
-
bioRxiv - Immunology 2023Quote: ... The IgG assay contained a goat anti-mouse IgG Fc antibody (SouthernBiotech, 1033-31, labeled with Alexa Fluor 488) and the IgM assay a donkey anti-mouse IgM F(ab’)2 (Jackson ImmunoResearch ...
-
bioRxiv - Immunology 2022Quote: ... the hACE2 receptor bound to the S protein was then detected using a mouse anti-His-Tag horseradish peroxidase conjugate (Southern Biotech) and a colorimetric signal generated by the addition of 3,3′,5,5′-TMB substrate ...
-
bioRxiv - Immunology 2022Quote: ... Cells were depleted of B cells and partially depleted of AMs and cells that had lost membrane integrity by first incubating with anti-IgD Biotin (Southern Biotech) and anti-Siglec-F Biotin ...
-
bioRxiv - Immunology 2023Quote: ... Bound IL-21 was subsequently detected with mouse anti-human IL-21-biotin (clone 2B2-G20, eBioscience) followed by alkaline phosphatase-conjugated streptavidin (Southern Biotech). The signals were compared to a standard curve generated with recombinant human IL-21 (R&D Systems).
-
bioRxiv - Cancer Biology 2020Quote: ... 1 × 105 dendritic cells or B cells were incubated with different concentrations of C03 with or without goat anti-human Fc antibody (SouthernBiotech) for 48 hours ...
-
bioRxiv - Cancer Biology 2020Quote: ... Jurkat/NF-κB-GFP-hCD40 reporter cells were incubated with different concentrations of CD40 agonist antibodies with or without goat anti-human Fc antibody (SouthernBiotech) for 24 hours ...
-
bioRxiv - Immunology 2020Quote: ... MagPlex beads were washed with PBST (PBS, 0.05% Tween-20) and incubated with anti-human IgG Fc-PE (Southern Biotech). Finally ...
-
bioRxiv - Immunology 2021Quote: ... at 1×106 cells/well and stimulated for 48 hours with 5 μg/mL of RBD-His or hCD81-His or media alone (RPMI + 5% FCS) in the presence of anti-mouse CD28 antibody [0,5 μg/ml] (Southern Biotech). As positive control cells were stimulated with anti-mouse CD3 [0,05 μg/ml] (Southern Biotech) ...
-
bioRxiv - Immunology 2020Quote: ... washed thrice with PBST and detected with HRP-conjugated mouse anti-human IgG (Fc only) (Southern Biotech, Birmingham, AL, USA) antibody at a 1:2000 dilution ...
-
bioRxiv - Neuroscience 2022Quote: ... The next day slides were washed and incubated for 1 hr in a secondary biotin-conjugated anti-mouse antibody (1:200, goat anti mouse, SouthernBiotech, Birmingham, AL) mixed in blocking solution ...
-
bioRxiv - Immunology 2023Quote: ... IgG3 was measured following 6 washes and a 1-h incubation with 100 μL/well 1:5,000 mouse anti-human biotin-conjugated IgG3 hinge (SouthernBiotech, Birmingham, Alabama, USA) then 6 washes and 1-h incubation with 100 μL/well 1:40,000 HRP-conjugated streptavidin (Jackson ImmunoResearch Labs) ...
-
bioRxiv - Immunology 2022Quote: ... Plates were washed with water three times before applying 25 μl of secondary antibody (goat anti-human IgG Fc-Southern Biotech, 2048-04 ...
-
bioRxiv - Microbiology 2021Quote: ... Plates were washed three times and incubated with the goat anti-human-IgG Fc secondary antibody conjugated with alkaline phosphatase (AP, Southern Biotech) at a 1:1000 dilution in blocking buffer for 1 h at room temperature ...
-
bioRxiv - Microbiology 2020Quote: ... followed by the addition of horseradish peroxidase-conjugated goat anti-mouse-IgG Fc secondary antibody (1:2000) (Southern Biotech, 1033-05). After incubation for 2 hours at room temperature ...
-
bioRxiv - Immunology 2019Quote: ... Plates were washed with PBS/0.1% Tween 20 and wells were incubated with alkaline phosphatase-conjugated anti-human Fc (Southern Biotech; P7998) followed by 2 hour incubation ...
-
bioRxiv - Immunology 2019Quote: ... Cells were washed with PBS–0.1% Tween 20 and incubated with mouse anti-human IgG Fc-HRP (Southern Biotech; 9040-05) for 1 hour ...
-
bioRxiv - Immunology 2019Quote: ... Plates were washed with PBS–0.1% Tween 20 and incubated with mouse anti-human IgG Fc-HRP (Southern Biotech; 9040-05) for 1 hour ...
-
bioRxiv - Microbiology 2023Quote: ... anti-IgG2 Fc-Alexa Fluor 488 and mouse anti-IgG3 hinge-Alexa Fluor 647 (1 µg/ml, all from Southern biotech) for an additional 20 minutes at 4°C ...