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Citations for SouthernBiotech :
51 - 100 of 187 citations for SARS CoV 2 Spike Glycoprotein S1 RBD Fc Tag CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... or 1:5,000 mouse anti-His-Tag (primary) then 1:5000 goat anti-mouse IgG(H+L)-HRP (secondary) (SouthernBiotech). Blots were developed with ECL Western Blotting Substrate (Pierce ...
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bioRxiv - Immunology 2019Quote: ... The plates were then washed and incubated with peroxidase-conjugated goat anti-mouse IgM Fc-specific (Southern Biotech). In differentiation assays ...
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bioRxiv - Biochemistry 2021Quote: ... and the secondary antibody solution was a 1:1200 dilution of goat-anti-human IgG Fc-HRP (SouthernBiotech). ELISA curves were fit to a 4-parameter logistic curve (Fig ...
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bioRxiv - Immunology 2024Quote: ... 50 μl of Goat anti-Human IgG Fc Multi-species SP ads-PE (Southern Biotech, catalog:2014-09) and Goat anti-Mouse IgG Fc Human ads-PE (Southern Biotech ...
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bioRxiv - Microbiology 2020Quote: ... membranes were washed with TBST and imaged (for Strep-Tag II HRP) or incubated with goat anti-rabbit-HRP (1:5,000; Southern Biotech).
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bioRxiv - Immunology 2023Quote: ... PE conjugated goat anti-human IgG Fc secondary Ab was added at 6μg/ml (Southern Biotech, catalog: 2014-09) and incubated for 1 hour at 37°C with shaking at 700 rpm ...
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bioRxiv - Immunology 2023Quote: ... The IgG assay contained a goat anti-mouse IgG Fc antibody (SouthernBiotech, 1033-31, labeled with Alexa Fluor 488) and the IgM assay a donkey anti-mouse IgM F(ab’)2 (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2022Quote: ... the hACE2 receptor bound to the S protein was then detected using a mouse anti-His-Tag horseradish peroxidase conjugate (Southern Biotech) and a colorimetric signal generated by the addition of 3,3′,5,5′-TMB substrate ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 × 105 dendritic cells or B cells were incubated with different concentrations of C03 with or without goat anti-human Fc antibody (SouthernBiotech) for 48 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... Jurkat/NF-κB-GFP-hCD40 reporter cells were incubated with different concentrations of CD40 agonist antibodies with or without goat anti-human Fc antibody (SouthernBiotech) for 24 hours ...
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bioRxiv - Immunology 2020Quote: ... MagPlex beads were washed with PBST (PBS, 0.05% Tween-20) and incubated with anti-human IgG Fc-PE (Southern Biotech). Finally ...
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bioRxiv - Immunology 2021Quote: ... at 1×106 cells/well and stimulated for 48 hours with 5 μg/mL of RBD-His or hCD81-His or media alone (RPMI + 5% FCS) in the presence of anti-mouse CD28 antibody [0,5 μg/ml] (Southern Biotech). As positive control cells were stimulated with anti-mouse CD3 [0,05 μg/ml] (Southern Biotech) ...
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bioRxiv - Immunology 2020Quote: ... washed thrice with PBST and detected with HRP-conjugated mouse anti-human IgG (Fc only) (Southern Biotech, Birmingham, AL, USA) antibody at a 1:2000 dilution ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed with water three times before applying 25 μl of secondary antibody (goat anti-human IgG Fc-Southern Biotech, 2048-04 ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed three times and incubated with the goat anti-human-IgG Fc secondary antibody conjugated with alkaline phosphatase (AP, Southern Biotech) at a 1:1000 dilution in blocking buffer for 1 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... followed by the addition of horseradish peroxidase-conjugated goat anti-mouse-IgG Fc secondary antibody (1:2000) (Southern Biotech, 1033-05). After incubation for 2 hours at room temperature ...
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bioRxiv - Immunology 2019Quote: ... Plates were washed with PBS/0.1% Tween 20 and wells were incubated with alkaline phosphatase-conjugated anti-human Fc (Southern Biotech; P7998) followed by 2 hour incubation ...
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bioRxiv - Immunology 2019Quote: ... Cells were washed with PBS–0.1% Tween 20 and incubated with mouse anti-human IgG Fc-HRP (Southern Biotech; 9040-05) for 1 hour ...
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bioRxiv - Immunology 2019Quote: ... Plates were washed with PBS–0.1% Tween 20 and incubated with mouse anti-human IgG Fc-HRP (Southern Biotech; 9040-05) for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... anti-IgG2 Fc-Alexa Fluor 488 and mouse anti-IgG3 hinge-Alexa Fluor 647 (1 µg/ml, all from Southern biotech) for an additional 20 minutes at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Surface-bound sLAP was detected with anti-human PE antibodies that bound directly to the Fc-IgG part of the chimeric sLAP proteins (SouthernBiotech, USA). PE signals were analyzed with FlowJo software (BD Biosciences ...
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bioRxiv - Biochemistry 2021Quote: ... 1×107 cells were starved for 30 min at 37°C in 1 ml FCS-free RPMI and then treated with 10 µg/ml anti-λ antibody (Southern Biotech) under shaking at 750 rpm for the indicated time points ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed twice in FACS buffer and stained with 50 μl/well of 1:200 dilution of R-phycoerythrin (PE)-conjugated mouse anti-human IgG Fc antibody (SouthernBiotech #9040-09) and 1:1000 dilution of Zombie-NIR viability dye (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... the cells were stained with 50μl/well of 1:200 diluted R-phycoerythrin (PE)-conjugated mouse anti-human IgG Fc antibody (SouthernBiotech cat.# 9040-09) and 1:1000 dilution of Zombie-NIR viability dye (BioLegend cat.# 423105 ...
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bioRxiv - Immunology 2020Quote: ... the plates were first washed 5 times with PBS-T and then incubated for 1 hour at RT with HRP-conjugated anti-human IgG (Fc-specific, clone JDC-10, Southern Biotech, Birmingham, AL) diluted 3000 × in PBS-T ...
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bioRxiv - Immunology 2020Quote: ... the plates were washed at least 5 times with PBS-T and then incubated for 1 hour at RT with HRP-conjugated anti-human IgG (Fc-specific, clone JDC-10, Southern Biotech, Birmingham, AL) diluted 3000 × in PBS-T ...
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bioRxiv - Biochemistry 2020Quote: ... the culture media were tested for their ACE2-Fc contents using a custom ELISA (anti-ACE2 antibody [Abcam, Cambridge, MA] for capturing and anti-human IgG-Fc-HRP [SouthernBiotech, Birmingham, AL] for detection). Clones with the highest expression of recombinant ACE2-Fc variants were selected ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed twice with Fc stain buffer and stained with Alexa Fluor 647 goat anti-human IgG (Southern Biotech [Birmingham, AL], cat. 2016-31) in FACS buffer for 30 minutes at 4°C followed by 2 wash steps with FC stain buffer and resuspended in Fluorofix (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... IgA (Clone 11-44-2, Southern Biotech), and IgM (Clone RMM-1 ...
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bioRxiv - Microbiology 2021Quote: ... The plates were washed a second time and incubated for 2 h with Streptavidin-alkaline phosphatase from Southern Biotech (2 µg/mL). The final wash was followed by the addition of Nitro-blue Tetrazolium Chloride/5-bromo-4-chloro 3 ‘indolyphosphate p-toludine salt (NBT/BCIP chromagen ...
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bioRxiv - Immunology 2024Quote: ... goat F(ab′)2 anti-human kappa FITC and goat F(ab′)2 anti-human lambda FITC (SouthernBiotech; to detect antibody expression), and PI (Invitrogen ...
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bioRxiv - Immunology 2021Quote: Goat F(ab’)2 anti-mouse Igκ (Southern Biotech) was biotinylated with 20-fold molar excess of NHS-LC-LC-biotin (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... (2) block sections in 5% normal goat serum (SouthernBiotech) and 0.3% Triton-X solution in PBS for 1h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... goat F(ab’)2 anti-mouse IgM (αIgM, SouthernBiotech) or rat anti-mouse CD40 (αCD40 ...
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bioRxiv - Cancer Biology 2020Quote: Primary CLL samples were treated with 20 µg/ml anti-IgM F(ab’)2 or control F(ab’)2 (both Southern Biotech, Cambridge Biosciences, UK) for 5 minutes at 37°C/5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... F(ab’)2 fragments or human IgG1 (control) (Southern Biotech) for 1hr at 37°C in a 96-well plate ...
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bioRxiv - Biochemistry 2020Quote: ... 6-diamidino-2-phenylindole (DAPI; Southern Biotech, Birmingham, AL, USA). Images were made using the Zeiss LSM880 (Oberkochen ...
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bioRxiv - Immunology 2022Quote: ... anti-IgG F(ab’)2 (5 μg/mL; Southern Biotech) or anti-IgM F(ab’)2 (5 μg/mL ...
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bioRxiv - Microbiology 2022Quote: ... α-IgA (mAb 11-44-2, FITC, SouthernBiotech catalog no. 1165-02). Cells were stained with primary antibodies (1:50 dilution ...
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bioRxiv - Immunology 2022Quote: ... or anti-IgM F(ab’)2 (5 μg/mL; Southern Biotech). Cells were immediately fixed with pre-warmed paraformaldehyde (4% final concentration) ...
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bioRxiv - Bioengineering 2019Quote: ... for 2 hr and mounted with Fluoromount-G (SouthernBiotech; 0100-20). Imaging was performed on a Nikon A1Rsi confocal microscope.
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bioRxiv - Immunology 2021Quote: ... followed by goat F(ab’)2 anti-mouse IgG1-FITC (Southern Biotech). The percentage of living HUCCT1 cells was estimated by flow cytometry ...
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bioRxiv - Immunology 2020Quote: ... Cells were stimulated with anti-mouse IgM-F(ab)2 (Southern Biotech), ionomycin (4μM ...
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bioRxiv - Microbiology 2022Quote: ... or 2 μg/ml goat anti-hu-IgM-FITC (2020-02, SouthernBiotech) for 30 minutes at 4 °C while shaking ...
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bioRxiv - Immunology 2023Quote: ... 20 µg/mL of F(ab’)2 anti-human IgM (Southern Biotech) was added to the cell medium and incubated for the indicated length of time ...
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bioRxiv - Cell Biology 2023Quote: ... goat F(ab’)2 anti-mouse IgG-PE (Southern Biotech, Birmingham, AL); and anti-phospho-AKT (Ser473) ...
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bioRxiv - Immunology 2024Quote: ... goat F(ab′)2 anti-human IgG PE (SouthernBiotech, dilution 1:100), and PI (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... Slides were mounted with DAPI Fluoromount-G (DAPI, 4,6-diamidino-2-phenylindole) (SouthernBiotech). Stained slides were imaged using Leica Application Suite X software on a Leica Sp8 (Leica) ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were mounted using 4,6-diamidino-2-phenylindole (DAPI) Fluoromount-G (SouthernBiotech) and sealed with a coverslip ...
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bioRxiv - Neuroscience 2022Quote: ... then 2 times with 1X PBS before mounting with Fluoromount-G (Southern Biotech) on slides with a 0.5mm spacer between the slide and coverslip ...