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Citations for SouthernBiotech :
201 - 250 of 269 citations for SARS CoV 2 Spike Glycoprotein S1 His tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Fluoromount-G used to mount cells for imaging was obtained from Southern Biotech (Cat# 0100-01). AZD1152 (AURKB inhibitor Cat# SML0268 ...
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bioRxiv - Bioengineering 2020Quote: ... cells were washed with PBS three times followed by treatment with DAPI Fluoromount-G (Southern Biotech). Cells were imaged with a Leica SP8 3×STED superresolution microscope.
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bioRxiv - Immunology 2019Quote: A20 D1.3 cells were stained on ice for 10 min with anti-IgM-biotin (Southern Biotech) or HEL-biotin and washed with PBS ...
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bioRxiv - Genomics 2023Quote: ... Cells were checked for mycoplasma contamination using the Mycoplasma detection kit (Southern Biotech, catalog #13100-01) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then washed with PBS and mounted on slides with Fluoromount G (SouthernBiotech #0100-01) for imaging and quantification using the CellInsight CX7 LED Pro High-Content Screening Platform (ThermoFisher Scientific HCSDCX7LEDPRO).
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bioRxiv - Cell Biology 2024Quote: ... Cells were regularly validated as mycoplasma free by a PCR-based mycoplasma detection kit (Southern Biotech).
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed again 3x with PBS before allowing to dry and mounting with Fluoromount-G (SouthernBioTech).
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bioRxiv - Immunology 2022Quote: ... Cells were further incubated with biotinylated goat anti-rabbit IgG (H+L) and HRP-streptavidin (Southern Biotech). The reactions were developed with o-Phenylenediamine di-hydrochloridec (OPD ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were collected and stained on ice with Alexa-fluor 488-coupled anti-IgA Ab (Southern Biotech) and 7AAD (Roche ...
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bioRxiv - Immunology 2020Quote: ... The cells were stained for surface expression of IgA using PE-conjugated anti-mouse IgA antibody (SouthernBiotech) and sorted by a FACS Aria IIIu and influx sorter (BD Biosciences) ...
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bioRxiv - Cell Biology 2020Quote: The apoptosis level of PC12 cells was determined by ApoScreen Annexin V kit (SouthernBiotech, Birmingham, AL, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed three times with 1x PBST and mounted with DAPI Fluoromount-G® (SouthernBiotech, AL).
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then washed and mounted on the slide glass using fluromount-G® (SouthernBiotech, 011-01).
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bioRxiv - Synthetic Biology 2023Quote: ... the cells were incubated with an anti-human IgG antibody conjugated to Alexa Fluor-488 (Southern Biotech Catalog# 2015-30 ...
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bioRxiv - Molecular Biology 2024Quote: ... Glass slides with the cells were washed with PBS and mounted with DAPI Fluoromount-G (SouthernBiotech, USA). LSM 510 META inverted confocal microscope at a 40× magnification (Carl Zeiss Micro Imaging ...
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bioRxiv - Microbiology 2024Quote: ... Cells were routinely confirmed to be mycoplasma free by conducting PCRs using a mycoplasma detection kit (SouthernBiotech).
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bioRxiv - Microbiology 2019Quote: ... This cell line was routinely tested for mycoplasma contamination using a Mycoplasma Detection Kit (SouthernBiotech, Cat# 13100-01).
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bioRxiv - Cell Biology 2021Quote: ... and coverslips were mounted on top of the cells using Fluoromount-G mounting solution containing DAPI (Southern Biotech). Single slice images were taken on a Zeiss 880 microscope using the confocal settings.
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bioRxiv - Microbiology 2020Quote: ... Cells were washed a further three times before mounting with Fluoromount G with DAPI (Cambridge Bioscience, Southern Biotech). Imaging was performed with an Axioscope 2 fluorescence microscope (Zeiss ...
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bioRxiv - Immunology 2020Quote: ... Cells were then surface stained with S/RBD probes and the following antibodies: IgD AF488 (polyclonal; Southern Biotech), IgM BUV395 (G20-127) ...
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bioRxiv - Neuroscience 2021Quote: ... cell nuclei were stained using DAPI during the first round of HCR-FISH staining (DAPI Fluoromount-G; SouthernBiotech).
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bioRxiv - Cancer Biology 2023Quote: ... Cells were routinely screened and confirmed negative for mycoplasma contamination at least every three months (SouthernBiotech #13100-01).
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bioRxiv - Microbiology 2023Quote: ... Cells were washed with PBS and incubated for 1 h with a secondary goat anti-mouse FITC (SouthernBiotech) at a 20 μg/mL concentration and phalloidin (7 units ...
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bioRxiv - Immunology 2023Quote: ... cover slips with stained cells were mounted with DAPI Fluoromount-G (Southern Biotech, AL, USA, Cat. 0100-20) on microscope slides and analyzed by an AxioObserver Z.1 Microscope with 40x or 63x objectives (Zeiss ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed 3 times with PBST and mounted on slides with Fluoromount-G (SouthernBiotech #0100-01) or ProLong™ Glass Antifade Mountant (Invitrogen P36984) ...
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bioRxiv - Cell Biology 2019Quote: ... The fixed cells were washed with PBS twice and resuspended in a mounting medium (Dapi Fluoromount-G; Southern Biotech). The samples were put on microscope slides ...
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bioRxiv - Biochemistry 2021Quote: ... Cells lines were authenticated and tested for mycoplasma contamination before use via the Mycoplasma Detection Kit (SouthernBiotech; 13100-01). Cells were maintained in a humidified atmosphere of 5% CO2 and 37 °C and were passaged every 2-3 days into 10 cm polystyrene coated plates (Eppendorf ...
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bioRxiv - Cell Biology 2021Quote: ... Afterward cells were fixed with 4% FA and mounted on glass slides using Fluoromount-G mounting medium (Southern Biotech). Images of the cells were acquired using confocal microscopy and analyzed with ZEN software (Carl Zeiss).
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bioRxiv - Cell Biology 2021Quote: ... Afterward cells were fixed with 4% FA and mounted on glass slides using Fluoromount-G mounting medium (Southern Biotech). Images of the cells were acquired using confocal microscopy and analyzed with ZEN software (Carl Zeiss).
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bioRxiv - Neuroscience 2020Quote: ... the cover slips with cultured cortical cells or slides with brain tissue were either mounted with Fluoromount-G (SouthernBiotech) or subjected to a subsequent immunocytochemistry ...
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bioRxiv - Physiology 2020Quote: ... Cells were also regularly checked for mycoplasma contamination using a PCR based mycoplasma detection kit from SouthernBiotech (Birmingham, AL) and were confirmed to be negative ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then surface stained with mouse anti-chicken Bu-1-FITC (Clone AV20; Southern Biotech, Birmingham, AL, USA) and mouse anti-chicken monocyte/macrophage-PE (Clone KUL01 ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed with 1% BSA-PBS and labeled with APC-conjugated goat anti-rat immunoglobulin antibody (Southern Biotech) at 25°C for 20 min ...
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bioRxiv - Immunology 2021Quote: ... The cells were washed with FACS buffer and incubated with secondary goat anti-human IgG PE antibody (Southern Biotech) for 1 hour at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... The cell nuclei were labeled with a DAPI stain present in the mounting media (DAPI Fluoromount-G, Southern Biotech). Images were acquired using an upright microscope (Echo Revolve ...
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bioRxiv - Immunology 2022Quote: ... cells secreting anti-NP antibody (ASC) were visualized with HRP-conjugated anti-mouse IgM or IgG3 antibodies (Southern Biotech) followed by AEC staining Kit (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed five times in PBS and mounted on glass slides using Fluoromount G (Southern Biotech; 0100-01). Prepared specimens were imaged on a Leica DMi8 (LAS X software ...
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bioRxiv - Cancer Biology 2020Quote: ... Jurkat/NF-κB-GFP-hCD40 reporter cells were incubated with different concentrations of CD40 agonist antibodies with or without goat anti-human Fc antibody (SouthernBiotech) for 24 hours ...
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bioRxiv - Immunology 2020Quote: ... 4 single cell clones of DT40 WT or Fam72a−/− were stained with anti-IgM-PE (1:40, clone M-1, SouthernBiotech) and 12 populations of 5×104 IgM− cells were sorted for each clone ...
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bioRxiv - Immunology 2022Quote: ... fixed blood cells were washed in bicarbonate buffer and stained using antibodies against CD71 (Southern Biotech, #1720-02, 1:200) and Ter119 (eBioscience ...
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bioRxiv - Microbiology 2020Quote: ... Cell lysates and supernatants were subjected to immunoblot analysis for antibodies against cellular LAMP-1 (1:2000, SouthernBiotech, 9835-01), VSV-G (1:2000 ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were then washed in 1xPBS 3x for 5mins and mounted in Fluoromount-G (Southern Biotech cat no 0100-01). Images were then acquired using a NikonA1R confocal microscope using a 60X ...
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bioRxiv - Immunology 2021Quote: ... the pellet was resuspended in a 50 μL mixture of PE-anti-human CD11b (Southern Biotech, 10 μL/million cells), Alexa Fluor 647-anti-human CD35 (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with FACs buffer (1% BSA, 1% Na+azide) and secondary antibody IgG-PE (Southern Biotech, Alabama, USA) was added in a 1:200 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed 3x with DPBS and coverslips mounted on 1 mm glass slides using Fluoromount-G (SouthernBiotech, #0100-01). Cells were imaged using a Zeiss Axio Observer 7 fitted with a 40x objective using DAPI and Alexa Fluor 488 filters ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were mounted on glass slides in a 1:1 ratio of YC complete:DAPI Fluoromount-G (SouthernBiotech, Cat #0100-20). Yeast were imaged with a Zeiss 880 LSM with fast Airyscan.
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bioRxiv - Molecular Biology 2023Quote: ... cells were fixed with 4% paraformaldehyde for 30 min at room temperature and stained with DAPI Fluoromount-G (Southern Biotech) to observe intracellular localization using an Olympus BX63 fluorescence microscope ...
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bioRxiv - Cell Biology 2023Quote: ... Cells cultured on glass coverslips were then lifted and mounted on microscope slides using Fluoromount-G Mounting Medium (Southern Biotech) and stored at 4C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... tissue slices and cell culture slices were covered by a glass slice with the mounting medium (SouthernBiotech, 0100-01, USA). Zeiss LSM800 (Zeiss ...
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bioRxiv - Immunology 2021Quote: ... and double-distilled water to remove cells before being incubated with alkaline phosphatase-conjugated anti-mouse IgM or anti-mouse IgG1 (Southern Biotech) for 2 h at room temperature ...